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Protein

Urocanate hydratase

Gene

hutU

Organism
Brucella abortus biovar 1 (strain 9-941)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of urocanate to 4-imidazolone-5-propionate.

Catalytic activityi

3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate = urocanate + H2O.

Cofactori

NAD+Note: Binds 1 NAD+ per subunit.

Pathwayi: L-histidine degradation into L-glutamate

This protein is involved in step 2 of the subpathway that synthesizes N-formimidoyl-L-glutamate from L-histidine.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Histidine ammonia-lyase (hutH)
  2. Urocanate hydratase (hutU)
  3. Imidazolonepropionase (hutI)
This subpathway is part of the pathway L-histidine degradation into L-glutamate, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N-formimidoyl-L-glutamate from L-histidine, the pathway L-histidine degradation into L-glutamate and in Amino-acid degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei130NAD1
Binding sitei196NAD1
Binding sitei201NAD1
Binding sitei322NAD; via carbonyl oxygen1
Active sitei4101
Binding sitei492NAD; via carbonyl oxygen1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi52 – 53NAD2
Nucleotide bindingi176 – 178NAD3
Nucleotide bindingi242 – 243NAD2
Nucleotide bindingi263 – 267NAD5
Nucleotide bindingi273 – 274NAD2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processHistidine metabolism
LigandNAD

Enzyme and pathway databases

UniPathwayiUPA00379; UER00550.

Names & Taxonomyi

Protein namesi
Recommended name:
Urocanate hydratase (EC:4.2.1.49)
Short name:
Urocanase
Alternative name(s):
Imidazolonepropionate hydrolase
Gene namesi
Name:hutU
Ordered Locus Names:BruAb2_0301
OrganismiBrucella abortus biovar 1 (strain 9-941)
Taxonomic identifieri262698 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
Proteomesi
  • UP000000540 Componenti: Chromosome II

Subcellular locationi

  • Cytoplasm

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000251251 – 557Urocanate hydrataseAdd BLAST557

Structurei

3D structure databases

ProteinModelPortaliQ579F0.
SMRiQ579F0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the urocanase family.

Phylogenomic databases

HOGENOMiHOG000237606.
KOiK01712.
OMAiVSFHHGG.

Family and domain databases

HAMAPiMF_00577. HutU. 1 hit.
InterProiView protein in InterPro
IPR023637. Urocanase.
IPR035401. Urocanase_C.
IPR023636. Urocanase_CS.
IPR035400. Urocanase_N.
IPR035085. Urocanase_Rossmann-like.
IPR036190. Urocanase_sf.
PANTHERiPTHR12216. PTHR12216. 1 hit.
PfamiView protein in Pfam
PF01175. Urocanase. 1 hit.
PF17392. Urocanase_C. 1 hit.
PF17391. Urocanase_N. 1 hit.
PIRSFiPIRSF001423. Urocanate_hydrat. 1 hit.
SUPFAMiSSF111326. SSF111326. 1 hit.
TIGRFAMsiTIGR01228. hutU. 1 hit.
PROSITEiView protein in PROSITE
PS01233. UROCANASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q579F0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNPRHNERE VRSPRGDELN AKSWLTEAPL RMLMNNLDPD VAERPHELVV
60 70 80 90 100
YGGIGRAART WDDFDRIVAT LKTLNDNETL LVQSGKPVGV FRTHKDAPRV
110 120 130 140 150
LIANSNLVPH WANWDHFNEL DKKGLAMYGQ MTAGSWIYIG AQGIVQGTYE
160 170 180 190 200
TFVEAGRQHY GGNLKGRWIL TGGLGGMGGA QPLAAVMAGA CCLAVECDET
210 220 230 240 250
RADFRLRTRY VDEKTHSLDE ALAKIDAWTK AGEAKSIALI GNAAEIFPEL
260 270 280 290 300
VKRGVKPDIV TDQTSAHDPV HGYLPLGWTV AEWRAKQEND PKAVEKAARA
310 320 330 340 350
SMKVQVQAML DFWNAGIPTV DYGNNIRQMA LEEGLENAFA FPGFVPAYIR
360 370 380 390 400
PLFCRGIGPY RWAALSGDPE DIAKTDAKVK ELLPDNKHLH NWLDMAKERI
410 420 430 440 450
AFQGLPARIC WVGLGDRHRL GLAFNEMVRN GELKAPIVIG RDHLDSGSVA
460 470 480 490 500
SPNRETEAMK DGSDAVSDWP LLNALLNTAS GATWVSLHHG GGVGMGFSQH
510 520 530 540 550
AGMVICCDGT EDADRRLERV LWNDPATGVM RHADAGYDIA LDWARKQGLR

LPAILGN
Length:557
Mass (Da):61,229
Last modified:May 10, 2005 - v1
Checksum:i98DC4B1A168ACAB4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017224 Genomic DNA. Translation: AAX75734.1.
RefSeqiWP_002965715.1. NC_006933.1.

Genome annotation databases

EnsemblBacteriaiAAX75734; AAX75734; BruAb2_0301.
KEGGibmb:BruAb2_0301.

Similar proteinsi

Entry informationi

Entry nameiHUTU_BRUAB
AccessioniPrimary (citable) accession number: Q579F0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 10, 2005
Last modified: November 22, 2017
This is version 78 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Brucella abortus strain 9-941
    Brucella abortus (strain 9-941): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families