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Reviewed, UniProtKB/Swiss-Prot Q579B1 (LEU3_BRUAB)

Last modified February 9, 2010. Version 34. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    3-isopropylmalate dehydrogenase
    EC=1.1.1.85
Alternative name(s):
    Beta-IPM dehydrogenase
      Short name=IMDH
    3-IPM-DH
Gene names
Name: leuB
Ordered Locus Names: BruAb2_0342
OrganismBrucella abortus [Complete proteome] [HAMAP]
Taxonomic identifier235 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

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Protein attributes

Sequence length370 AA.
Sequence statusComplete.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate By similarity. HAMAP MF_01033

Catalytic activity

(2R,3S)-3-isopropylmalate + NAD+ = 4-methyl-2-oxopentanoate + CO2 + NADH. HAMAP MF_01033

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity. HAMAP MF_01033

Pathway

Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4. HAMAP MF_01033

Subunit structure

Homodimer By similarity. HAMAP MF_01033

Subcellular location

Cytoplasm By similarity HAMAP MF_01033.

Sequence similarities

Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3703703-isopropylmalate dehydrogenase HAMAP MF_01033
PRO_0000250105

Regions

Nucleotide binding77 – 9014NAD By similarity
Nucleotide binding290 – 30213NAD By similarity

Sites

Metal binding2261Magnesium or manganese By similarity
Metal binding2501Magnesium or manganese By similarity
Metal binding2541Magnesium or manganese By similarity
Binding site971Substrate By similarity
Binding site1071Substrate By similarity
Binding site1351Substrate By similarity
Binding site2261Substrate By similarity
Site1421Important for catalysis By similarity
Site1931Important for catalysis By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q579B1-1 [UniParc].

Last modified May 10, 2005. Version 1.
Checksum: 5A389C4E26E82DFF

FASTA37039,730
        10         20         30         40         50         60 
MASRKLLLLP GDGIGPEAMA EVRKVIAFLN SDLNLGFETE EGLVGGCAYD AHGQAISDAD 

        70         80         90        100        110        120 
MEKALAADSV LFGAVGGPKW DSVPYEVRPE AGLLRLRKDM QLYANLRPAI CYPALAHSSS 

       130        140        150        160        170        180 
LKPEVIEGLD ILILRELTGG VYFGEPKEII DLGNGQKRGI DTQVYDTYEI ERIADVAFEL 

       190        200        210        220        230        240 
ARTRRNKVTS MEKRNVMKSG VLWNQGVTAR HKEKHADVQL EHMLADAGGM QLVRWPKQFD 

       250        260        270        280        290        300 
VILTDNLFGD LLSDVAAMLT GSLGMLPSAS LGAADSKTGK RKALYEPVHG SAPDIAGKGI 

       310        320        330        340        350        360 
ANPIAMIASL AMCLRYSFGL VAEADRLEAA IAGVLDDGIR TADIWSEGNT KVGTTEMGDA 

       370 
ILAKFKALSA

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References

[1]"Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis."
Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z., Li L.-L., Kapur V., Alt D.P., Olsen S.C.
J. Bacteriol. 187:2715-2726(2005) [PubMed: 15805518] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 9-941 / Biovar 1.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE017224 Genomic DNA. Translation: AAX75773.1.
RefSeqYP_223134.1.

3D structure databases

HSSPHSSP built from PDB template 1A05 based on UniProtKB Q56268.
SMRQ579B1. Positions 4-365.
ModBaseSearch...

Genome annotation databases

GeneID3341711.
GenomeReviewsGene locus BruAb2_0342 in contig AE017224_GR.
KEGGbmb:BruAb2_0342.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG518924.
OMAMSYQIAV.
PhylomeDBQ579B1.

Enzyme and pathway databases

BioCycBABO262698:BRUAB2_0342-MONOMER.
BRENDA1.1.1.85. 575.

Family and domain databases

HAMAPMF_01033. LeuB_type1.
[Tree]
InterProIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR004429. Isopropylmalate_DH.
[Graphical view]
Gene3DG3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHERPTHR11835. IDH_IMDH_dimeric. 1 hit.
PTHR11835:SF13. IPMDH. 1 hit.
PfamPF00180. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsTIGR00169. leuB. 1 hit.
PROSITEPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameLEU3_BRUAB
AccessionPrimary (citable) accession number: Q579B1
Entry history
Integrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: May 10, 2005
Last modified: February 9, 2010
This is version 34 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Brucella abortus strain 9-941

Brucella abortus (strain 9-941): entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents