Reviewed, UniProtKB/Swiss-Prot Q57991 (AK_METJA)

Names and origin

Protein names

Recommended name:
    Probable aspartokinase
    EC=2.7.2.4
Alternative name(s):
    Aspartate kinase

Gene names

Ordered Locus Names:

MJ0571

Organism

Methanocaldococcus jannaschii (Methanococcus jannaschii) [Complete proteome] [HAMAP]

Taxonomic identifier

2190 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	473 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + L-aspartate = ADP + 4-phospho-L-aspartate.
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 1/4. Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 1/3. Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 1/5.
Sequence similarities	Belongs to the aspartokinase family. Contains 2 ACT domains.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Threonine biosynthesis
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	threonine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW amino acid binding Inferred from electronic annotation. Source: InterPro aspartate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 473

473

Probable aspartokinase

PRO_0000066688

Regions

Domain

322 – 389

68

ACT 1

Domain

408 – 473

66

ACT 2

Secondary structure

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473

Helix Strand Turn

Details...

Beta strand

3 – 7

5

Helix

10 – 12

3

Helix

15 – 31

17

Beta strand

35 – 39

5

Helix

45 – 58

14

Helix

62 – 83

22

Helix

87 – 114

28

Helix

119 – 126

8

Helix

128 – 143

16

Beta strand

148 – 151

4

Turn

153 – 157

5

Beta strand

158 – 160

3

Beta strand

169 – 172

4

Helix

174 – 183

10

Beta strand

187 – 196

10

Beta strand

202 – 204

3

Turn

207 – 209

3

Helix

210 – 221

12

Beta strand

224 – 234

11

Beta strand

236 – 238

3

Turn

240 – 242

3

Beta strand

250 – 253

4

Helix

254 – 262

9

Helix

270 – 272

3

Helix

273 – 278

6

Beta strand

283 – 287

5

Beta strand

296 – 300

5

Beta strand

306 – 308

3

Beta strand

311 – 325

15

Beta strand

327 – 329

3

Helix

331 – 344

14

Beta strand

349 – 354

6

Beta strand

361 – 365

5

Helix

370 – 381

12

Beta strand

395 – 410

16

Turn

412 – 416

5

Helix

420 – 430

11

Beta strand

437 – 442

6

Beta strand

444 – 452

9

Helix

453 – 455

3

Helix

456 – 467

12

Sequences

	Sequence		Length	Mass (Da)
	Q57991-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 811C6E0F4B66BC5F	FASTA	473	51,392
	10 20 30 40 50 60 MTTVMKFGGT SVGSGERIRH VAKIVTKRKK EDDDVVVVVS AMSEVTNALV EISQQALDVR 70 80 90 100 110 120 DIAKVGDFIK FIREKHYKAI EEAIKSEEIK EEVKKIIDSR IEELEKVLIG VAYLGELTPK 130 140 150 160 170 180 SRDYILSFGE RLSSPILSGA IRDLGEKSIA LEGGEAGIIT DNNFGSARVK RLEVKERLLP 190 200 210 220 230 240 LLKEGIIPVV TGFIGTTEEG YITTLGRGGS DYSAALIGYG LDADIIEIWT DVSGVYTTDP 250 260 270 280 290 300 RLVPTARRIP KLSYIEAMEL AYFGAKVLHP RTIEPAMEKG IPILVKNTFE PESEGTLITN 310 320 330 340 350 360 DMEMSDSIVK AISTIKNVAL INIFGAGMVG VSGTAARIFK ALGEEEVNVI LISQGSSETN 370 380 390 400 410 420 ISLVVSEEDV DKALKALKRE FGDFGKKSFL NNNLIRDVSV DKDVCVISVV GAGMRGAKGI 430 440 450 460 470 AGKIFTAVSE SGANIKMIAQ GSSEVNISFV IDEKDLLNCV RKLHEKFIEK TNS

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

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Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L77117 Genomic DNA. Translation: AAB98565.1.

PIR

C64371.

RefSeq

NP_247550.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2HMF	X-ray	2.70	A/B/C/D	2-470	[»]
3C1M	X-ray	2.30	A/B/C/D	1-473	[»]
3C1N	X-ray	2.72	A/B/C/D	1-473	[»]
3C20	X-ray	2.70	A/B	1-473	[»]

ModBase

Search...

Genome annotation databases

GeneID

1451436.

GenomeReviews

Gene locus MJ0571 in contig L77117_GR.

KEGG

mja:MJ0571.

NMPDR

fig|243232.1.peg.587.

TIGR

MJ0571.

Phylogenomic databases

HOGENOM

HBG724395.

OMA

GSSETNI.

Enzyme and pathway databases

BRENDA

2.7.2.4. 256362.

Family and domain databases

InterPro

IPR002912. ACT_bd.
IPR001048. Asp/Glu/Uridylate_kinase.
IPR005260. Asp_kin_monofn.
IPR001341. Asp_kinase_dom.
IPR018042. Aspartate_kinase_CS.
[Graphical view]

Gene3D

G3DSA:3.40.1160.10. Aa_kinase. 1 hit.

Pfam

PF00696. AA_kinase. 1 hit.
PF01842. ACT. 2 hits.
[Graphical view]

TIGRFAMs

TIGR00656. asp_kin_monofn. 1 hit.
TIGR00657. asp_kinases. 1 hit.

PROSITE

PS00324. ASPARTOKINASE. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

AK_METJA

Accession

Primary (citable) accession number: Q57991

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	February 9, 2010
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)