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Protein

Probable aspartokinase

Gene

MJ0571

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-aspartate = ADP + 4-phospho-L-aspartate.

Pathwayi: L-lysine biosynthesis via DAP pathway

This protein is involved in step 1 of the subpathway that synthesizes (S)-tetrahydrodipicolinate from L-aspartate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Probable aspartokinase (MJ0571)
  2. Aspartate-semialdehyde dehydrogenase (asd)
  3. 4-hydroxy-tetrahydrodipicolinate synthase (dapA)
  4. 4-hydroxy-tetrahydrodipicolinate reductase (dapB)
This subpathway is part of the pathway L-lysine biosynthesis via DAP pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-tetrahydrodipicolinate from L-aspartate, the pathway L-lysine biosynthesis via DAP pathway and in Amino-acid biosynthesis.

Pathwayi: L-methionine biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes L-homoserine from L-aspartate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Probable aspartokinase (MJ0571)
  2. Aspartate-semialdehyde dehydrogenase (asd)
  3. Homoserine dehydrogenase (hom)
This subpathway is part of the pathway L-methionine biosynthesis via de novo pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-homoserine from L-aspartate, the pathway L-methionine biosynthesis via de novo pathway and in Amino-acid biosynthesis.

Pathwayi: L-threonine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-threonine from L-aspartate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Probable aspartokinase (MJ0571)
  2. Aspartate-semialdehyde dehydrogenase (asd)
  3. Homoserine dehydrogenase (hom)
  4. Homoserine kinase (thrB)
  5. Threonine synthase (thrC)
This subpathway is part of the pathway L-threonine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-threonine from L-aspartate, the pathway L-threonine biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Threonine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.2.4. 3260.
UniPathwayiUPA00034; UER00015.
UPA00050; UER00461.
UPA00051; UER00462.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable aspartokinase (EC:2.7.2.4)
Alternative name(s):
Aspartate kinase
Gene namesi
Ordered Locus Names:MJ0571
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
Proteomesi
  • UP000000805 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 473473Probable aspartokinasePRO_0000066688Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi243232.MJ_0571.

Structurei

Secondary structure

1
473
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 75Combined sources
Turni9 – 124Combined sources
Helixi15 – 2915Combined sources
Beta strandi35 – 395Combined sources
Helixi45 – 5814Combined sources
Helixi62 – 8322Combined sources
Helixi87 – 11428Combined sources
Helixi119 – 14325Combined sources
Beta strandi148 – 1514Combined sources
Helixi153 – 1564Combined sources
Beta strandi158 – 1603Combined sources
Beta strandi169 – 1724Combined sources
Helixi174 – 18310Combined sources
Beta strandi187 – 19711Combined sources
Beta strandi202 – 2043Combined sources
Turni207 – 2093Combined sources
Helixi210 – 22011Combined sources
Beta strandi224 – 23411Combined sources
Beta strandi236 – 2383Combined sources
Turni240 – 2423Combined sources
Beta strandi250 – 2534Combined sources
Helixi254 – 2629Combined sources
Beta strandi265 – 2673Combined sources
Helixi270 – 2723Combined sources
Helixi273 – 2797Combined sources
Beta strandi283 – 2875Combined sources
Beta strandi296 – 3005Combined sources
Beta strandi306 – 3083Combined sources
Beta strandi311 – 32515Combined sources
Beta strandi327 – 3293Combined sources
Helixi331 – 34414Combined sources
Beta strandi349 – 3546Combined sources
Beta strandi361 – 3666Combined sources
Helixi367 – 3693Combined sources
Helixi370 – 38112Combined sources
Beta strandi390 – 3923Combined sources
Beta strandi395 – 41016Combined sources
Turni412 – 4165Combined sources
Helixi420 – 43112Combined sources
Beta strandi437 – 4426Combined sources
Beta strandi444 – 4529Combined sources
Helixi453 – 4553Combined sources
Helixi456 – 46712Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2HMFX-ray2.70A/B/C/D2-470[»]
3C1MX-ray2.30A/B/C/D1-473[»]
3C1NX-ray2.72A/B/C/D1-473[»]
3C20X-ray2.70A/B1-473[»]
ProteinModelPortaliQ57991.
SMRiQ57991. Positions 2-470.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ57991.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini323 – 39270ACT 1PROSITE-ProRule annotationAdd
BLAST
Domaini409 – 47365ACT 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the aspartokinase family.Curated
Contains 2 ACT domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiarCOG00861. Archaea.
COG0527. LUCA.
InParanoidiQ57991.
KOiK00928.
OMAiGANIKMI.
PhylomeDBiQ57991.

Family and domain databases

Gene3Di3.40.1160.10. 2 hits.
InterProiIPR002912. ACT_dom.
IPR001048. Asp/Glu/Uridylate_kinase.
IPR005260. Asp_kin_monofn.
IPR001341. Asp_kinase_dom.
IPR018042. Aspartate_kinase_CS.
IPR027795. GATS-like_ACT_dom.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
PF01842. ACT. 1 hit.
PF13840. ACT_7. 1 hit.
[Graphical view]
PIRSFiPIRSF000726. Asp_kin. 1 hit.
SUPFAMiSSF53633. SSF53633. 1 hit.
TIGRFAMsiTIGR00656. asp_kin_monofn. 1 hit.
TIGR00657. asp_kinases. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
PS00324. ASPARTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q57991-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTVMKFGGT SVGSGERIRH VAKIVTKRKK EDDDVVVVVS AMSEVTNALV
60 70 80 90 100
EISQQALDVR DIAKVGDFIK FIREKHYKAI EEAIKSEEIK EEVKKIIDSR
110 120 130 140 150
IEELEKVLIG VAYLGELTPK SRDYILSFGE RLSSPILSGA IRDLGEKSIA
160 170 180 190 200
LEGGEAGIIT DNNFGSARVK RLEVKERLLP LLKEGIIPVV TGFIGTTEEG
210 220 230 240 250
YITTLGRGGS DYSAALIGYG LDADIIEIWT DVSGVYTTDP RLVPTARRIP
260 270 280 290 300
KLSYIEAMEL AYFGAKVLHP RTIEPAMEKG IPILVKNTFE PESEGTLITN
310 320 330 340 350
DMEMSDSIVK AISTIKNVAL INIFGAGMVG VSGTAARIFK ALGEEEVNVI
360 370 380 390 400
LISQGSSETN ISLVVSEEDV DKALKALKRE FGDFGKKSFL NNNLIRDVSV
410 420 430 440 450
DKDVCVISVV GAGMRGAKGI AGKIFTAVSE SGANIKMIAQ GSSEVNISFV
460 470
IDEKDLLNCV RKLHEKFIEK TNS
Length:473
Mass (Da):51,392
Last modified:November 1, 1996 - v1
Checksum:i811C6E0F4B66BC5F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB98565.1.
PIRiC64371.

Genome annotation databases

EnsemblBacteriaiAAB98565; AAB98565; MJ_0571.
KEGGimja:MJ_0571.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB98565.1.
PIRiC64371.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2HMFX-ray2.70A/B/C/D2-470[»]
3C1MX-ray2.30A/B/C/D1-473[»]
3C1NX-ray2.72A/B/C/D1-473[»]
3C20X-ray2.70A/B1-473[»]
ProteinModelPortaliQ57991.
SMRiQ57991. Positions 2-470.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243232.MJ_0571.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB98565; AAB98565; MJ_0571.
KEGGimja:MJ_0571.

Phylogenomic databases

eggNOGiarCOG00861. Archaea.
COG0527. LUCA.
InParanoidiQ57991.
KOiK00928.
OMAiGANIKMI.
PhylomeDBiQ57991.

Enzyme and pathway databases

UniPathwayiUPA00034; UER00015.
UPA00050; UER00461.
UPA00051; UER00462.
BRENDAi2.7.2.4. 3260.

Miscellaneous databases

EvolutionaryTraceiQ57991.

Family and domain databases

Gene3Di3.40.1160.10. 2 hits.
InterProiIPR002912. ACT_dom.
IPR001048. Asp/Glu/Uridylate_kinase.
IPR005260. Asp_kin_monofn.
IPR001341. Asp_kinase_dom.
IPR018042. Aspartate_kinase_CS.
IPR027795. GATS-like_ACT_dom.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
PF01842. ACT. 1 hit.
PF13840. ACT_7. 1 hit.
[Graphical view]
PIRSFiPIRSF000726. Asp_kin. 1 hit.
SUPFAMiSSF53633. SSF53633. 1 hit.
TIGRFAMsiTIGR00656. asp_kin_monofn. 1 hit.
TIGR00657. asp_kinases. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
PS00324. ASPARTOKINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Entry informationi

Entry nameiAK_METJA
AccessioniPrimary (citable) accession number: Q57991
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: December 9, 2015
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.