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Protein

Adenylosuccinate synthetase

Gene

purA

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP.UniRule annotation

Catalytic activityi

GTP + IMP + L-aspartate = GDP + phosphate + N6-(1,2-dicarboxyethyl)-AMP.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: AMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes AMP from IMP.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Adenylosuccinate synthetase (purA)
  2. Adenylosuccinate lyase (purB)
This subpathway is part of the pathway AMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes AMP from IMP, the pathway AMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei19Proton acceptorUniRule annotation1
Metal bindingi19MagnesiumUniRule annotation1
Metal bindingi48Magnesium; via carbonyl oxygenUniRule annotation1
Active sitei49Proton donorUniRule annotation1
Binding sitei133IMPUniRule annotation1
Binding sitei147IMP; shared with dimeric partnerUniRule annotation1
Binding sitei185IMPUniRule annotation1
Binding sitei200IMPUniRule annotation1
Binding sitei262IMPUniRule annotation1
Binding sitei264GTPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi18 – 24GTPUniRule annotation7
Nucleotide bindingi48 – 50GTPUniRule annotation3
Nucleotide bindingi290 – 292GTPUniRule annotation3
Nucleotide bindingi330 – 332GTPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processPurine biosynthesis
LigandGTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.3.4.4 3260
UniPathwayiUPA00075; UER00335

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylosuccinate synthetaseUniRule annotation (EC:6.3.4.4UniRule annotation)
Short name:
AMPSaseUniRule annotation
Short name:
AdSSUniRule annotation
Alternative name(s):
IMP--aspartate ligaseUniRule annotation
Gene namesi
Name:purAUniRule annotation
Ordered Locus Names:MJ0561
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
Proteomesi
  • UP000000805 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000952711 – 345Adenylosuccinate synthetaseAdd BLAST345

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi243232.MJ_0561

Structurei

3D structure databases

ProteinModelPortaliQ57981
SMRiQ57981
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni19 – 22IMP bindingUniRule annotation4
Regioni46 – 49IMP bindingUniRule annotation4
Regioni258 – 264Substrate bindingUniRule annotation7

Sequence similaritiesi

Belongs to the adenylosuccinate synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04387 Archaea
COG0104 LUCA
InParanoidiQ57981
KOiK01939
OMAiSNAGHTV
OrthoDBiPOG093Z058B
PhylomeDBiQ57981

Family and domain databases

HAMAPiMF_00011 Adenylosucc_synth, 1 hit
InterProiView protein in InterPro
IPR018220 Adenylosuccin_syn_GTP-bd
IPR001114 Adenylosuccinate_synthetase
IPR027417 P-loop_NTPase
PANTHERiPTHR11846 PTHR11846, 3 hits
PfamiView protein in Pfam
PF00709 Adenylsucc_synt, 1 hit
SMARTiView protein in SMART
SM00788 Adenylsucc_synt, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS01266 ADENYLOSUCCIN_SYN_1, 1 hit

Sequencei

Sequence statusi: Complete.

Q57981-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKVVLLTCT IIVGGQWGDE GKGKIISYIC DKDKPSIIAR GGVGPNAGHT
60 70 80 90 100
VNIGGKSYGI RMIPTGFPYK EAKLAIGAGV LVDPEVLLKE VEMLKDFNVK
110 120 130 140 150
ERLIVDYRCG IIEEKHKIMD RKDEHLAKEI GTTGSGCGPA NVDRVLRILK
160 170 180 190 200
QAKDIEELKE FLGDVSEEVN NALDRGENVL IEGTQGTLLS LYYGTYPYVT
210 220 230 240 250
SKDTTASSFA ADVGIGPTKV DEVIVVFKTF PTRVGAGPFP TEMSLEEAES
260 270 280 290 300
LGIVEYGTVT GRRRRVGYFD FELARKACRL NGATQIALTG LDKYDKECYG
310 320 330 340
VTEYNKLSEK AKEFINKIEE VTGVPVTIIS TGPEMHQTID LRNEL
Length:345
Mass (Da):37,820
Last modified:November 1, 1996 - v1
Checksum:i9974DBC30D1DEE72
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA Translation: AAB98554.1
PIRiA64370

Genome annotation databases

EnsemblBacteriaiAAB98554; AAB98554; MJ_0561
KEGGimja:MJ_0561

Similar proteinsi

Entry informationi

Entry nameiPURA_METJA
AccessioniPrimary (citable) accession number: Q57981
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: March 28, 2018
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health