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Q57981

- PURA_METJA

UniProt

Q57981 - PURA_METJA

Protein

Adenylosuccinate synthetase

Gene

purA

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP By similarity.By similarity

    Catalytic activityi

    GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP.UniRule annotation

    Cofactori

    Binds 1 magnesium ion per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei19 – 191Proton acceptorUniRule annotation
    Metal bindingi19 – 191MagnesiumUniRule annotation
    Metal bindingi48 – 481Magnesium; via carbonyl oxygenUniRule annotation
    Active sitei49 – 491Proton donorUniRule annotation
    Binding sitei133 – 1331IMPUniRule annotation
    Binding sitei147 – 1471IMP; shared with dimeric partnerUniRule annotation
    Binding sitei185 – 1851IMPUniRule annotation
    Binding sitei200 – 2001IMPUniRule annotation
    Binding sitei262 – 2621IMPUniRule annotation
    Binding sitei264 – 2641GTPUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi18 – 247GTPUniRule annotation
    Nucleotide bindingi48 – 503GTPUniRule annotation
    Nucleotide bindingi290 – 2923GTPUniRule annotation
    Nucleotide bindingi330 – 3323GTPUniRule annotation

    GO - Molecular functioni

    1. adenylosuccinate synthase activity Source: UniProtKB-HAMAP
    2. GTP binding Source: UniProtKB-HAMAP
    3. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. 'de novo' AMP biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Purine biosynthesis

    Keywords - Ligandi

    GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00075; UER00335.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Adenylosuccinate synthetaseUniRule annotation (EC:6.3.4.4UniRule annotation)
    Short name:
    AMPSaseUniRule annotation
    Short name:
    AdSSUniRule annotation
    Alternative name(s):
    IMP--aspartate ligaseUniRule annotation
    Gene namesi
    Name:purAUniRule annotation
    Ordered Locus Names:MJ0561
    OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
    Taxonomic identifieri243232 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
    ProteomesiUP000000805: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 345345Adenylosuccinate synthetasePRO_0000095271Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi243232.MJ0561.

    Structurei

    3D structure databases

    ProteinModelPortaliQ57981.
    SMRiQ57981. Positions 11-343.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni19 – 224IMP bindingUniRule annotation
    Regioni46 – 494IMP bindingUniRule annotation
    Regioni258 – 2647Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the adenylosuccinate synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0104.
    KOiK01939.
    OMAiMKRAVQI.
    PhylomeDBiQ57981.

    Family and domain databases

    HAMAPiMF_00011. Adenylosucc_synth.
    InterProiIPR018220. Adenylosuccinate_synthase_AS.
    IPR001114. Adenylosuccinate_synthetase.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PANTHERiPTHR11846. PTHR11846. 1 hit.
    PfamiPF00709. Adenylsucc_synt. 1 hit.
    [Graphical view]
    SMARTiSM00788. Adenylsucc_synt. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS01266. ADENYLOSUCCIN_SYN_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q57981-1 [UniParc]FASTAAdd to Basket

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    MKKVVLLTCT IIVGGQWGDE GKGKIISYIC DKDKPSIIAR GGVGPNAGHT    50
    VNIGGKSYGI RMIPTGFPYK EAKLAIGAGV LVDPEVLLKE VEMLKDFNVK 100
    ERLIVDYRCG IIEEKHKIMD RKDEHLAKEI GTTGSGCGPA NVDRVLRILK 150
    QAKDIEELKE FLGDVSEEVN NALDRGENVL IEGTQGTLLS LYYGTYPYVT 200
    SKDTTASSFA ADVGIGPTKV DEVIVVFKTF PTRVGAGPFP TEMSLEEAES 250
    LGIVEYGTVT GRRRRVGYFD FELARKACRL NGATQIALTG LDKYDKECYG 300
    VTEYNKLSEK AKEFINKIEE VTGVPVTIIS TGPEMHQTID LRNEL 345
    Length:345
    Mass (Da):37,820
    Last modified:November 1, 1996 - v1
    Checksum:i9974DBC30D1DEE72
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L77117 Genomic DNA. Translation: AAB98554.1.
    PIRiA64370.
    RefSeqiNP_247540.1. NC_000909.1.
    WP_010870065.1. NC_000909.1.

    Genome annotation databases

    EnsemblBacteriaiAAB98554; AAB98554; MJ_0561.
    GeneIDi1451426.
    KEGGimja:MJ_0561.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L77117 Genomic DNA. Translation: AAB98554.1 .
    PIRi A64370.
    RefSeqi NP_247540.1. NC_000909.1.
    WP_010870065.1. NC_000909.1.

    3D structure databases

    ProteinModelPortali Q57981.
    SMRi Q57981. Positions 11-343.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243232.MJ0561.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAB98554 ; AAB98554 ; MJ_0561 .
    GeneIDi 1451426.
    KEGGi mja:MJ_0561.

    Phylogenomic databases

    eggNOGi COG0104.
    KOi K01939.
    OMAi MKRAVQI.
    PhylomeDBi Q57981.

    Enzyme and pathway databases

    UniPathwayi UPA00075 ; UER00335 .

    Family and domain databases

    HAMAPi MF_00011. Adenylosucc_synth.
    InterProi IPR018220. Adenylosuccinate_synthase_AS.
    IPR001114. Adenylosuccinate_synthetase.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    PANTHERi PTHR11846. PTHR11846. 1 hit.
    Pfami PF00709. Adenylsucc_synt. 1 hit.
    [Graphical view ]
    SMARTi SM00788. Adenylsucc_synt. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS01266. ADENYLOSUCCIN_SYN_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

    Entry informationi

    Entry nameiPURA_METJA
    AccessioniPrimary (citable) accession number: Q57981
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 102 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Methanococcus jannaschii
      Methanococcus jannaschii: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3