Q57981 (PURA_METJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenylosuccinate synthetase
Short name=AMPSase
Short name=AdSS
EC=6.3.4.4

Alternative name(s):
IMP--aspartate ligase

Gene names

Name:	purA
Ordered Locus Names:	MJ0561

Organism

Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)

Taxonomic identifier

243232 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP By similarity. HAMAP MF_00011
Catalytic activity	GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP. HAMAP MF_00011
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00011
Pathway	Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2. HAMAP MF_00011
Subunit structure	Homodimer By similarity. HAMAP MF_00011
Subcellular location	Cytoplasm By similarity HAMAP MF_00011.
Sequence similarities	Belongs to the adenylosuccinate synthetase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	purine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW adenylosuccinate synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 345	345	Adenylosuccinate synthetase HAMAP MF_00011		PRO_0000095271
Regions
	Nucleotide binding	18 – 24	7	GTP By similarity
	Nucleotide binding	48 – 50	3	GTP By similarity
	Nucleotide binding	290 – 292	3	GTP By similarity
	Nucleotide binding	330 – 332	3	GTP By similarity
	Region	19 – 22	4	IMP binding By similarity
	Region	46 – 49	4	IMP binding By similarity
	Region	258 – 264	7	Substrate binding By similarity
Sites
	Active site	19	1	Proton acceptor By similarity
	Active site	49	1	Proton donor By similarity
	Metal binding	19	1	Magnesium By similarity
	Metal binding	48	1	Magnesium; via carbonyl oxygen By similarity
	Binding site	133	1	IMP By similarity
	Binding site	147	1	IMP; shared with dimeric partner By similarity
	Binding site	185	1	IMP By similarity
	Binding site	200	1	IMP By similarity
	Binding site	262	1	IMP By similarity
	Binding site	264	1	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q57981 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 9974DBC30D1DEE72	FASTA	345	37,820
	10 20 30 40 50 60 MKKVVLLTCT IIVGGQWGDE GKGKIISYIC DKDKPSIIAR GGVGPNAGHT VNIGGKSYGI 70 80 90 100 110 120 RMIPTGFPYK EAKLAIGAGV LVDPEVLLKE VEMLKDFNVK ERLIVDYRCG IIEEKHKIMD 130 140 150 160 170 180 RKDEHLAKEI GTTGSGCGPA NVDRVLRILK QAKDIEELKE FLGDVSEEVN NALDRGENVL 190 200 210 220 230 240 IEGTQGTLLS LYYGTYPYVT SKDTTASSFA ADVGIGPTKV DEVIVVFKTF PTRVGAGPFP 250 260 270 280 290 300 TEMSLEEAES LGIVEYGTVT GRRRRVGYFD FELARKACRL NGATQIALTG LDKYDKECYG 310 320 330 340 VTEYNKLSEK AKEFINKIEE VTGVPVTIIS TGPEMHQTID LRNEL

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L77117 Genomic DNA. Translation: AAB98554.1.
PIR	A64370.
RefSeq	NP_247540.1. NC_000909.1.
3D structure databases
ProteinModelPortal	Q57981.
SMR	Q57981. Positions 11-343.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1451426.
GenomeReviews	Gene locus MJ0561 in contig L77117_GR.
KEGG	mja:MJ_0561.
NMPDR	fig|243232.1.peg.577.
TIGR	MJ0561.
Phylogenomic databases
HOGENOM	HBG658237.
OMA	YVLGIIK.
ProtClustDB	PRK04293.
Enzyme and pathway databases
BioCyc	MJAN243232:MJ_0561-MONOMER.
Family and domain databases
HAMAP	MF_00011. Adenylosucc_synth. [Tree]
InterPro	IPR018220. Adenylosuccinate_synthase_AS. IPR001114. Adenylosuccinate_synthetase. [Graphical view]
KO	K01939.
PANTHER	PTHR11846. Asucc_synthtase. 1 hit.
Pfam	PF00709. Adenylsucc_synt. 1 hit. [Graphical view]
SMART	SM00788. Adenylsucc_synt. 1 hit. [Graphical view]
PROSITE	PS01266. ADENYLOSUCCIN_SYN_1. 1 hit. PS00513. ADENYLOSUCCIN_SYN_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PURA_METJA

Accession

Primary (citable) accession number: Q57981

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	November 16, 2011
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program