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Q57981

- PURA_METJA

UniProt

Q57981 - PURA_METJA

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Protein

Adenylosuccinate synthetase

Gene
purA, MJ0561
Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP By similarity.UniRule annotation

Catalytic activityi

GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP.UniRule annotation

Cofactori

Binds 1 magnesium ion per subunit By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei19 – 191Proton acceptor By similarity
Metal bindingi19 – 191Magnesium By similarity
Metal bindingi48 – 481Magnesium; via carbonyl oxygen By similarity
Active sitei49 – 491Proton donor By similarity
Binding sitei133 – 1331IMP By similarity
Binding sitei147 – 1471IMP; shared with dimeric partner By similarity
Binding sitei185 – 1851IMP By similarity
Binding sitei200 – 2001IMP By similarity
Binding sitei262 – 2621IMP By similarity
Binding sitei264 – 2641GTP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi18 – 247GTP By similarity
Nucleotide bindingi48 – 503GTP By similarity
Nucleotide bindingi290 – 2923GTP By similarity
Nucleotide bindingi330 – 3323GTP By similarity

GO - Molecular functioni

  1. adenylosuccinate synthase activity Source: UniProtKB-HAMAP
  2. GTP binding Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. 'de novo' AMP biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00075; UER00335.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylosuccinate synthetase (EC:6.3.4.4)
Short name:
AMPSase
Short name:
AdSS
Alternative name(s):
IMP--aspartate ligase
Gene namesi
Name:purA
Ordered Locus Names:MJ0561
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
ProteomesiUP000000805: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 345345Adenylosuccinate synthetaseUniRule annotationPRO_0000095271Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi243232.MJ0561.

Structurei

3D structure databases

ProteinModelPortaliQ57981.
SMRiQ57981. Positions 11-343.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni19 – 224IMP binding By similarity
Regioni46 – 494IMP binding By similarity
Regioni258 – 2647Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0104.
KOiK01939.
OMAiMKRAVQI.
PhylomeDBiQ57981.

Family and domain databases

HAMAPiMF_00011. Adenylosucc_synth.
InterProiIPR018220. Adenylosuccinate_synthase_AS.
IPR001114. Adenylosuccinate_synthetase.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11846. PTHR11846. 1 hit.
PfamiPF00709. Adenylsucc_synt. 1 hit.
[Graphical view]
SMARTiSM00788. Adenylsucc_synt. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS01266. ADENYLOSUCCIN_SYN_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q57981-1 [UniParc]FASTAAdd to Basket

« Hide

MKKVVLLTCT IIVGGQWGDE GKGKIISYIC DKDKPSIIAR GGVGPNAGHT    50
VNIGGKSYGI RMIPTGFPYK EAKLAIGAGV LVDPEVLLKE VEMLKDFNVK 100
ERLIVDYRCG IIEEKHKIMD RKDEHLAKEI GTTGSGCGPA NVDRVLRILK 150
QAKDIEELKE FLGDVSEEVN NALDRGENVL IEGTQGTLLS LYYGTYPYVT 200
SKDTTASSFA ADVGIGPTKV DEVIVVFKTF PTRVGAGPFP TEMSLEEAES 250
LGIVEYGTVT GRRRRVGYFD FELARKACRL NGATQIALTG LDKYDKECYG 300
VTEYNKLSEK AKEFINKIEE VTGVPVTIIS TGPEMHQTID LRNEL 345
Length:345
Mass (Da):37,820
Last modified:November 1, 1996 - v1
Checksum:i9974DBC30D1DEE72
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L77117 Genomic DNA. Translation: AAB98554.1.
PIRiA64370.
RefSeqiNP_247540.1. NC_000909.1.
WP_010870065.1. NC_000909.1.

Genome annotation databases

EnsemblBacteriaiAAB98554; AAB98554; MJ_0561.
GeneIDi1451426.
KEGGimja:MJ_0561.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L77117 Genomic DNA. Translation: AAB98554.1 .
PIRi A64370.
RefSeqi NP_247540.1. NC_000909.1.
WP_010870065.1. NC_000909.1.

3D structure databases

ProteinModelPortali Q57981.
SMRi Q57981. Positions 11-343.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 243232.MJ0561.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAB98554 ; AAB98554 ; MJ_0561 .
GeneIDi 1451426.
KEGGi mja:MJ_0561.

Phylogenomic databases

eggNOGi COG0104.
KOi K01939.
OMAi MKRAVQI.
PhylomeDBi Q57981.

Enzyme and pathway databases

UniPathwayi UPA00075 ; UER00335 .

Family and domain databases

HAMAPi MF_00011. Adenylosucc_synth.
InterProi IPR018220. Adenylosuccinate_synthase_AS.
IPR001114. Adenylosuccinate_synthetase.
IPR027417. P-loop_NTPase.
[Graphical view ]
PANTHERi PTHR11846. PTHR11846. 1 hit.
Pfami PF00709. Adenylsucc_synt. 1 hit.
[Graphical view ]
SMARTi SM00788. Adenylsucc_synt. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
PROSITEi PS01266. ADENYLOSUCCIN_SYN_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Entry informationi

Entry nameiPURA_METJA
AccessioniPrimary (citable) accession number: Q57981
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: September 3, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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