Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q57962

- PPSA_METJA

UniProt

Q57962 - PPSA_METJA

Protein

Probable phosphoenolpyruvate synthase

Gene

ppsA

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate.By similarity

    Catalytic activityi

    ATP + pyruvate + H2O = AMP + phosphoenolpyruvate + phosphate.

    Cofactori

    Magnesium.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei824 – 8241Tele-phosphohistidine intermediateBy similarity
    Binding sitei917 – 9171SubstrateBy similarity
    Binding sitei964 – 9641SubstrateBy similarity
    Metal bindingi1061 – 10611MagnesiumBy similarity
    Binding sitei1061 – 10611SubstrateBy similarity
    Binding sitei1083 – 10831Substrate; via carbonyl oxygenBy similarity
    Binding sitei1084 – 10841Substrate; via amide nitrogenBy similarity
    Binding sitei1085 – 10851SubstrateBy similarity
    Metal bindingi1086 – 10861MagnesiumBy similarity
    Binding sitei1086 – 10861Substrate; via amide nitrogenBy similarity
    Active sitei1133 – 11331Proton donorBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. endonuclease activity Source: InterPro
    3. metal ion binding Source: UniProtKB-KW
    4. pyruvate, water dikinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. gluconeogenesis Source: UniProtKB-UniPathway
    2. intein-mediated protein splicing Source: InterPro

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00138.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable phosphoenolpyruvate synthase (EC:2.7.9.2)
    Short name:
    PEP synthase
    Alternative name(s):
    Pyruvate, water dikinase
    Cleaved into the following chain:
    Alternative name(s):
    Mja pepA intein
    Gene namesi
    Name:ppsA
    Ordered Locus Names:MJ0542
    OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
    Taxonomic identifieri243232 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
    ProteomesiUP000000805: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 410410Probable phosphoenolpyruvate synthase, 1st partSequence AnalysisPRO_0000023556Add
    BLAST
    Chaini411 – 822412Mja pep inteinSequence AnalysisPRO_0000023557Add
    BLAST
    Chaini823 – 1188366Probable phosphoenolpyruvate synthase, 2nd partSequence AnalysisPRO_0000023558Add
    BLAST

    Post-translational modificationi

    This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.Curated

    Keywords - PTMi

    Autocatalytic cleavage, Phosphoprotein, Protein splicing

    Interactioni

    Protein-protein interaction databases

    STRINGi243232.MJ0542.

    Structurei

    3D structure databases

    ProteinModelPortaliQ57962.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini536 – 670135DOD-type homing endonucleasePROSITE-ProRule annotationAdd
    BLAST

    Domaini

    The N-terminal domain contains the ATP/Pi binding site, the central domain the pyrophosphate/phosphate carrier histidine, and the C-terminal domain the pyruvate binding site.By similarity

    Sequence similaritiesi

    Belongs to the PEP-utilizing enzyme family.Curated
    Contains 1 DOD-type homing endonuclease domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0574.
    KOiK01007.
    OMAiERVKECW.

    Family and domain databases

    Gene3Di3.10.28.10. 1 hit.
    3.20.20.60. 1 hit.
    3.30.1490.20. 1 hit.
    3.30.470.20. 1 hit.
    3.50.30.10. 2 hits.
    InterProiIPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR028992. Hedgehog/Intein_dom.
    IPR003587. Hint_dom_N.
    IPR027434. Homing_endonucl.
    IPR004042. Intein_endonuc.
    IPR006141. Intein_splice_site.
    IPR008279. PEP-util_enz_mobile_dom.
    IPR000121. PEP_util_C.
    IPR023151. PEP_util_CS.
    IPR006318. PEP_util_enz.
    IPR002192. PPDK_PEP-bd.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    [Graphical view]
    PfamiPF00391. PEP-utilizers. 2 hits.
    PF02896. PEP-utilizers_C. 1 hit.
    PF01326. PPDK_N. 1 hit.
    [Graphical view]
    PRINTSiPR01736. PHPHTRNFRASE.
    SMARTiSM00306. HintN. 1 hit.
    [Graphical view]
    SUPFAMiSSF51294. SSF51294. 2 hits.
    SSF51621. SSF51621. 1 hit.
    SSF52009. SSF52009. 1 hit.
    SSF55608. SSF55608. 1 hit.
    TIGRFAMsiTIGR01443. intein_Cterm. 1 hit.
    TIGR01445. intein_Nterm. 1 hit.
    PROSITEiPS50818. INTEIN_C_TER. 1 hit.
    PS50819. INTEIN_ENDONUCLEASE. 1 hit.
    PS50817. INTEIN_N_TER. 1 hit.
    PS00742. PEP_ENZYMES_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q57962-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLIIQNTKGD SMKFIAWLDE LSNKDVDIAG GKGASLGEMW NAGLPVPPAF     50
    VVTADAYRHF IKETGLMDKI REILSGLDVN DTDALTNASK KIRKLIEEAE 100
    MPEDLRLAII EAYNKLCEMC GEDEVTVAVR SSATAEDLPE ASFAGQQDTY 150
    LNIKGAENVV KYVQKCFSSL FTPRAIFYRE QQGFDHFKVA LAAVVQKLVN 200
    AEKAGVMFTV NPISENYDEL VIEAAWGLGE GVVSGSVSPD TYIVNKKTLE 250
    IVDKHIARKE TMFVKDEKGE TKVVEVPDDM KEKQVLSDDE IKELAKIGLN 300
    IEKHYGKPMD VEWAYEKGKF YMLQARPITT LKKGKKEKKA KEEDIEAKIL 350
    LKGIGASPGI ATGVVKIIHD VSEIDKVKEG DILVTEMTTP DMVPAMKKAA 400
    AIVTDEGGLT CIEGDAKILT DRGFLKMKEV YKLVKNGEKL KVLGLNAETL 450
    KTEWKEIIDA QKREARRYEI GVYRKNKNTK DTIKITPDHK FPVFVNGELS 500
    KVQLCDIIDN NLSVLSIDYI PMIEEKYESL AEVMYLGGAV LSDGHIVRRN 550
    GKPIRVRFTQ KDTEEKKDFI EKVKGDVKLI GGNFIEISNR NNVIEYQTSR 600
    KIPSEILGFI EVNINTIPLY ATKDEIADLI AGFVDGDGCL SGKRRVEIYQ 650
    NSSHIKKIEG LIVGLYRLGI IPRLRYKRSS TATIYFNNNL ETILQRTRRI 700
    KLDKLKEFKK PVEDKKLIDI SQILPELKEF DYKGYLYKTY KEKLFIGINK 750
    LEEYLSKIDK DGIERIKQKI KLLKESDIYS IRIKKVGEDY GEVYNITVKA 800
    ENEFNHNYVV WTKHYTPIVV FNCHAAIVSR ELGTPCVVGT KKATKVLKDG 850
    MIVTVDGEKG IVYEGEIKKV EEKEKKQEVV VQQAPIITAT EVKVNVSMPE 900
    VAERAAATGA DGVGLLRAEH MILGLGKHPR KILEEEGEEA LIEALMEGIR 950
    KVADAFYPRP VTYRTLDAPT DEFRGLEGGE NEPIEHNPML GWRGIRRDLD 1000
    EVDILKCELK AIKRLREEGY KNIEIMIPLV THPDEVRRVK EIMREVGLEP 1050
    CKDIPFGIMV ETPAAALIIE DFIKEGINFV SLGTNDLTQY TIAIDRNNEL 1100
    VSKYYKEDHP AVLKLVEHVI KTCKKHGIKT SICGQAGSRP HIVEKLVEWG 1150
    IDSVSANIDA VETIRRVVAR TEQKVILNYI RKSYVERE 1188
    Length:1,188
    Mass (Da):134,012
    Last modified:November 1, 1996 - v1
    Checksum:i5547E2E2A8C46AEC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L77117 Genomic DNA. Translation: AAB98534.1.
    PIRiF64367.
    RefSeqiNP_247521.1. NC_000909.1.

    Genome annotation databases

    EnsemblBacteriaiAAB98534; AAB98534; MJ_0542.
    GeneIDi1451407.
    KEGGimja:MJ_0542.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L77117 Genomic DNA. Translation: AAB98534.1 .
    PIRi F64367.
    RefSeqi NP_247521.1. NC_000909.1.

    3D structure databases

    ProteinModelPortali Q57962.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243232.MJ0542.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAB98534 ; AAB98534 ; MJ_0542 .
    GeneIDi 1451407.
    KEGGi mja:MJ_0542.

    Phylogenomic databases

    eggNOGi COG0574.
    KOi K01007.
    OMAi ERVKECW.

    Enzyme and pathway databases

    UniPathwayi UPA00138 .

    Family and domain databases

    Gene3Di 3.10.28.10. 1 hit.
    3.20.20.60. 1 hit.
    3.30.1490.20. 1 hit.
    3.30.470.20. 1 hit.
    3.50.30.10. 2 hits.
    InterProi IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR028992. Hedgehog/Intein_dom.
    IPR003587. Hint_dom_N.
    IPR027434. Homing_endonucl.
    IPR004042. Intein_endonuc.
    IPR006141. Intein_splice_site.
    IPR008279. PEP-util_enz_mobile_dom.
    IPR000121. PEP_util_C.
    IPR023151. PEP_util_CS.
    IPR006318. PEP_util_enz.
    IPR002192. PPDK_PEP-bd.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    [Graphical view ]
    Pfami PF00391. PEP-utilizers. 2 hits.
    PF02896. PEP-utilizers_C. 1 hit.
    PF01326. PPDK_N. 1 hit.
    [Graphical view ]
    PRINTSi PR01736. PHPHTRNFRASE.
    SMARTi SM00306. HintN. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51294. SSF51294. 2 hits.
    SSF51621. SSF51621. 1 hit.
    SSF52009. SSF52009. 1 hit.
    SSF55608. SSF55608. 1 hit.
    TIGRFAMsi TIGR01443. intein_Cterm. 1 hit.
    TIGR01445. intein_Nterm. 1 hit.
    PROSITEi PS50818. INTEIN_C_TER. 1 hit.
    PS50819. INTEIN_ENDONUCLEASE. 1 hit.
    PS50817. INTEIN_N_TER. 1 hit.
    PS00742. PEP_ENZYMES_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

    Entry informationi

    Entry nameiPPSA_METJA
    AccessioniPrimary (citable) accession number: Q57962
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 107 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The reaction takes place in three steps, mediated by a phosphocarrier histidine residue located on the surface of the central domain. The two first partial reactions are catalyzed at an active site located on the N-terminal domain, and the third partial reaction is catalyzed at an active site located on the C-terminal domain. For catalytic turnover, the central domain swivels from the concave surface of the N-terminal domain to that of the C-terminal domain By similarity.By similarity

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Intein-containing proteins
      List of intein-containing protein entries
    2. Methanococcus jannaschii
      Methanococcus jannaschii: entries and gene names
    3. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3