Reviewed, UniProtKB/Swiss-Prot Q57962 (PPSA_METJA)

Names and origin

Protein names

Recommended name:
    Probable phosphoenolpyruvate synthase
      Short name=PEP synthase
    EC=2.7.9.2
Alternative name(s):
    Pyruvate, water dikinase
Cleaved into the following chain:
    1- Recommended name:
            Mja pep intein
        Alternative name(s):
            Mja pepA intein

Gene names

Name:	ppsA
Ordered Locus Names:	MJ0542

Organism

Methanocaldococcus jannaschii (Methanococcus jannaschii) [Complete proteome] [HAMAP]

Taxonomic identifier

2190 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	1188 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate By similarity.
Catalytic activity	ATP + pyruvate + H2O = AMP + phosphoenolpyruvate + phosphate.
Cofactor	Magnesium By similarity.
Pathway	Carbohydrate biosynthesis; gluconeogenesis.
Domain	The N-terminal domain contains the ATP/Pi binding site, the central domain the pyrophosphate/phosphate carrier histidine, and the C-terminal domain the pyruvate binding site By similarity.
Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential.
Miscellaneous	The reaction takes place in three steps, mediated by a phosphocarrier histidine residue located on the surface of the central domain. The two first partial reactions are catalyzed at an active site located on the N-terminal domain, and the third partial reaction is catalyzed at an active site located on the C-terminal domain. For catalytic turnover, the central domain swivels from the concave surface of the N-terminal domain to that of the C-terminal domain By similarity.
Sequence similarities	Belongs to the PEP-utilizing enzyme family. Contains 1 DOD-type homing endonuclease domain.

Ontologies

Keywords
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Autocatalytic cleavage Phosphoprotein Protein splicing
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from electronic annotation. Source: InterPro intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro phosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW endonuclease activity Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW pyruvate, water dikinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 410	410	Probable phosphoenolpyruvate synthase, 1st part Potential		PRO_0000023556
	Chain	411 – 822	412	Mja pep intein Potential		PRO_0000023557
	Chain	823 – 1188	366	Probable phosphoenolpyruvate synthase, 2nd part Potential		PRO_0000023558
Regions
	Domain	536 – 670	135	DOD-type homing endonuclease
Sites
	Active site	824	1	Tele-phosphohistidine intermediate By similarity
	Active site	1133	1	Proton donor By similarity
	Metal binding	1061	1	Magnesium By similarity
	Metal binding	1086	1	Magnesium By similarity
	Binding site	917	1	Substrate By similarity
	Binding site	964	1	Substrate By similarity
	Binding site	1061	1	Substrate By similarity
	Binding site	1083	1	Substrate; via carbonyl oxygen By similarity
	Binding site	1084	1	Substrate; via amide nitrogen By similarity
	Binding site	1085	1	Substrate By similarity
	Binding site	1086	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q57962-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 5547E2E2A8C46AEC	FASTA	1,188	134,012
	10 20 30 40 50 60 MLIIQNTKGD SMKFIAWLDE LSNKDVDIAG GKGASLGEMW NAGLPVPPAF VVTADAYRHF 70 80 90 100 110 120 IKETGLMDKI REILSGLDVN DTDALTNASK KIRKLIEEAE MPEDLRLAII EAYNKLCEMC 130 140 150 160 170 180 GEDEVTVAVR SSATAEDLPE ASFAGQQDTY LNIKGAENVV KYVQKCFSSL FTPRAIFYRE 190 200 210 220 230 240 QQGFDHFKVA LAAVVQKLVN AEKAGVMFTV NPISENYDEL VIEAAWGLGE GVVSGSVSPD 250 260 270 280 290 300 TYIVNKKTLE IVDKHIARKE TMFVKDEKGE TKVVEVPDDM KEKQVLSDDE IKELAKIGLN 310 320 330 340 350 360 IEKHYGKPMD VEWAYEKGKF YMLQARPITT LKKGKKEKKA KEEDIEAKIL LKGIGASPGI 370 380 390 400 410 420 ATGVVKIIHD VSEIDKVKEG DILVTEMTTP DMVPAMKKAA AIVTDEGGLT CIEGDAKILT 430 440 450 460 470 480 DRGFLKMKEV YKLVKNGEKL KVLGLNAETL KTEWKEIIDA QKREARRYEI GVYRKNKNTK 490 500 510 520 530 540 DTIKITPDHK FPVFVNGELS KVQLCDIIDN NLSVLSIDYI PMIEEKYESL AEVMYLGGAV 550 560 570 580 590 600 LSDGHIVRRN GKPIRVRFTQ KDTEEKKDFI EKVKGDVKLI GGNFIEISNR NNVIEYQTSR 610 620 630 640 650 660 KIPSEILGFI EVNINTIPLY ATKDEIADLI AGFVDGDGCL SGKRRVEIYQ NSSHIKKIEG 670 680 690 700 710 720 LIVGLYRLGI IPRLRYKRSS TATIYFNNNL ETILQRTRRI KLDKLKEFKK PVEDKKLIDI 730 740 750 760 770 780 SQILPELKEF DYKGYLYKTY KEKLFIGINK LEEYLSKIDK DGIERIKQKI KLLKESDIYS 790 800 810 820 830 840 IRIKKVGEDY GEVYNITVKA ENEFNHNYVV WTKHYTPIVV FNCHAAIVSR ELGTPCVVGT 850 860 870 880 890 900 KKATKVLKDG MIVTVDGEKG IVYEGEIKKV EEKEKKQEVV VQQAPIITAT EVKVNVSMPE 910 920 930 940 950 960 VAERAAATGA DGVGLLRAEH MILGLGKHPR KILEEEGEEA LIEALMEGIR KVADAFYPRP 970 980 990 1000 1010 1020 VTYRTLDAPT DEFRGLEGGE NEPIEHNPML GWRGIRRDLD EVDILKCELK AIKRLREEGY 1030 1040 1050 1060 1070 1080 KNIEIMIPLV THPDEVRRVK EIMREVGLEP CKDIPFGIMV ETPAAALIIE DFIKEGINFV 1090 1100 1110 1120 1130 1140 SLGTNDLTQY TIAIDRNNEL VSKYYKEDHP AVLKLVEHVI KTCKKHGIKT SICGQAGSRP 1150 1160 1170 1180 HIVEKLVEWG IDSVSANIDA VETIRRVVAR TEQKVILNYI RKSYVERE

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L77117 Genomic DNA. Translation: AAB98534.1.
PIR	F64367.
RefSeq	NP_247521.1.
3D structure databases
HSSP	HSSP built from PDB template 1DIK based on UniProtKB P22983.
ModBase	Search...
Genome annotation databases
GeneID	1451407.
GenomeReviews	Gene locus MJ0542 in contig L77117_GR.
KEGG	mja:MJ0542.
NMPDR	fig|243232.1.peg.558.
TIGR	MJ0542.
Phylogenomic databases
HOGENOM	Q57962.
OMA	RAEHMIL.
Enzyme and pathway databases
BioCyc	MJAN243232:MJ_0542-MON.
BRENDA	2.7.9.2. 256362.
Family and domain databases
InterPro	IPR013815. ATP_grasp_subdomain_1. IPR003587. Hedgehog_hint_N. IPR004042. Intein_endonuc. IPR006141. Intein_splicing_site. IPR008279. PEP_mobile. IPR018274. PEP_mobile_CS. IPR006318. PEP_P_trans. IPR006319. PEP_synth. IPR000121. PEP_utilizers. IPR002192. PPDK_PEP_bd. IPR015813. Pyrv/PenolPyrv_Kinase_cat. [Graphical view]
Gene3D	G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.50.30.10. PEP_mobile. 2 hits. G3DSA:3.20.20.60. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
Pfam	PF00391. PEP-utilizers. 2 hits. PF02896. PEP-utilizers_C. 1 hit. PF01326. PPDK_N. 1 hit. [Graphical view]
PRINTS	PR01736. PHPHTRNFRASE.
ProDom	PD000940. PEP_utilizers. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00306. HintN. 1 hit. [Graphical view]
TIGRFAMs	TIGR01443. intein_Cterm. 1 hit. TIGR01445. intein_Nterm. 1 hit. TIGR01418. PEP_synth. 1 hit.
PROSITE	PS50818. INTEIN_C_TER. 1 hit. PS50819. INTEIN_ENDONUCLEASE. 1 hit. PS50817. INTEIN_N_TER. 1 hit. PS00742. PEP_ENZYMES_2. 1 hit. PS00370. PEP_ENZYMES_PHOS_SITE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PPSA_METJA

Accession

Primary (citable) accession number: Q57962

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	November 3, 2009
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)