Probable phosphoenolpyruvate synthase - Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Probable phosphoenolpyruvate synthase
Short name=PEP synthase
EC=2.7.9.2

Alternative name(s):
Pyruvate, water dikinase

Cleaved into the following chain:

Mja pep intein
Alternative name(s):
Mja pepA intein

Gene names

Name:	ppsA
Ordered Locus Names:	MJ0542

Organism

Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) [Reference proteome] [HAMAP]

Taxonomic identifier

243232 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus ›

Protein attributes

Sequence length	1188 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate By similarity.
Catalytic activity	ATP + pyruvate + H₂O = AMP + phosphoenolpyruvate + phosphate.
Cofactor	Magnesium By similarity.
Pathway	Carbohydrate biosynthesis; gluconeogenesis.
Domain	The N-terminal domain contains the ATP/Pi binding site, the central domain the pyrophosphate/phosphate carrier histidine, and the C-terminal domain the pyruvate binding site By similarity.
Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential.
Miscellaneous	The reaction takes place in three steps, mediated by a phosphocarrier histidine residue located on the surface of the central domain. The two first partial reactions are catalyzed at an active site located on the N-terminal domain, and the third partial reaction is catalyzed at an active site located on the C-terminal domain. For catalytic turnover, the central domain swivels from the concave surface of the N-terminal domain to that of the C-terminal domain By similarity.
Sequence similarities	Belongs to the PEP-utilizing enzyme family. Contains 1 DOD-type homing endonuclease domain.

Ontologies

Keywords
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Autocatalytic cleavage Phosphoprotein Protein splicing
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	gluconeogenesis Inferred from electronic annotation. Source: UniProtKB-UniPathway intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW endonuclease activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW pyruvate, water dikinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 410

410

Probable phosphoenolpyruvate synthase, 1st part Potential

PRO_0000023556

Chain

411 – 822

412

Mja pep intein Potential

PRO_0000023557

Chain

823 – 1188

366

Probable phosphoenolpyruvate synthase, 2nd part Potential

PRO_0000023558

Regions

Domain

536 – 670

135

DOD-type homing endonuclease

Sites

Active site

824

1

Tele-phosphohistidine intermediate By similarity

Active site

1133

1

Proton donor By similarity

Metal binding

1061

1

Magnesium By similarity

Metal binding

1086

1

Magnesium By similarity

Binding site

917

1

Substrate By similarity

Binding site

964

1

Substrate By similarity

Binding site

1061

1

Substrate By similarity

Binding site

1083

1

Substrate; via carbonyl oxygen By similarity

Binding site

1084

1

Substrate; via amide nitrogen By similarity

Binding site

1085

1

Substrate By similarity

Binding site

1086

1

Substrate; via amide nitrogen By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q57962 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 5547E2E2A8C46AEC
Show »

FASTA

1,188

134,012

        10         20         30         40         50         60 
MLIIQNTKGD SMKFIAWLDE LSNKDVDIAG GKGASLGEMW NAGLPVPPAF VVTADAYRHF 

        70         80         90        100        110        120 
IKETGLMDKI REILSGLDVN DTDALTNASK KIRKLIEEAE MPEDLRLAII EAYNKLCEMC 

       130        140        150        160        170        180 
GEDEVTVAVR SSATAEDLPE ASFAGQQDTY LNIKGAENVV KYVQKCFSSL FTPRAIFYRE 

       190        200        210        220        230        240 
QQGFDHFKVA LAAVVQKLVN AEKAGVMFTV NPISENYDEL VIEAAWGLGE GVVSGSVSPD 

       250        260        270        280        290        300 
TYIVNKKTLE IVDKHIARKE TMFVKDEKGE TKVVEVPDDM KEKQVLSDDE IKELAKIGLN 

       310        320        330        340        350        360 
IEKHYGKPMD VEWAYEKGKF YMLQARPITT LKKGKKEKKA KEEDIEAKIL LKGIGASPGI 

       370        380        390        400        410        420 
ATGVVKIIHD VSEIDKVKEG DILVTEMTTP DMVPAMKKAA AIVTDEGGLT CIEGDAKILT 

       430        440        450        460        470        480 
DRGFLKMKEV YKLVKNGEKL KVLGLNAETL KTEWKEIIDA QKREARRYEI GVYRKNKNTK 

       490        500        510        520        530        540 
DTIKITPDHK FPVFVNGELS KVQLCDIIDN NLSVLSIDYI PMIEEKYESL AEVMYLGGAV 

       550        560        570        580        590        600 
LSDGHIVRRN GKPIRVRFTQ KDTEEKKDFI EKVKGDVKLI GGNFIEISNR NNVIEYQTSR 

       610        620        630        640        650        660 
KIPSEILGFI EVNINTIPLY ATKDEIADLI AGFVDGDGCL SGKRRVEIYQ NSSHIKKIEG 

       670        680        690        700        710        720 
LIVGLYRLGI IPRLRYKRSS TATIYFNNNL ETILQRTRRI KLDKLKEFKK PVEDKKLIDI 

       730        740        750        760        770        780 
SQILPELKEF DYKGYLYKTY KEKLFIGINK LEEYLSKIDK DGIERIKQKI KLLKESDIYS 

       790        800        810        820        830        840 
IRIKKVGEDY GEVYNITVKA ENEFNHNYVV WTKHYTPIVV FNCHAAIVSR ELGTPCVVGT 

       850        860        870        880        890        900 
KKATKVLKDG MIVTVDGEKG IVYEGEIKKV EEKEKKQEVV VQQAPIITAT EVKVNVSMPE 

       910        920        930        940        950        960 
VAERAAATGA DGVGLLRAEH MILGLGKHPR KILEEEGEEA LIEALMEGIR KVADAFYPRP 

       970        980        990       1000       1010       1020 
VTYRTLDAPT DEFRGLEGGE NEPIEHNPML GWRGIRRDLD EVDILKCELK AIKRLREEGY 

      1030       1040       1050       1060       1070       1080 
KNIEIMIPLV THPDEVRRVK EIMREVGLEP CKDIPFGIMV ETPAAALIIE DFIKEGINFV 

      1090       1100       1110       1120       1130       1140 
SLGTNDLTQY TIAIDRNNEL VSKYYKEDHP AVLKLVEHVI KTCKKHGIKT SICGQAGSRP 

      1150       1160       1170       1180 
HIVEKLVEWG IDSVSANIDA VETIRRVVAR TEQKVILNYI RKSYVERE

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References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii."
Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G.

Venter J.C.
Science 273:1058-1073(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L77117 Genomic DNA. Translation: AAB98534.1.
PIR	F64367.
RefSeq	NP_247521.1. NC_000909.1.
3D structure databases
ProteinModelPortal	Q57962.
ModBase	Search...
Protein-protein interaction databases
STRING	243232.MJ0542.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAB98534; AAB98534; MJ_0542.
GeneID	1451407.
KEGG	mja:MJ_0542.
Phylogenomic databases
eggNOG	COG0574.
KO	K01007.
OMA	TYLNICG.
ProtClustDB	CLSK2749892.
Enzyme and pathway databases
UniPathway	UPA00138.
Family and domain databases
Gene3D	3.20.20.60. 1 hit. 3.30.1490.20. 1 hit. 3.30.470.20. 1 hit.
InterPro	IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR003587. Hint_dom_N. IPR004042. Intein_endonuc. IPR006141. Intein_splice_site. IPR008279. PEP-util_enz_mobile_dom. IPR000121. PEP_util_C. IPR023151. PEP_util_CS. IPR006318. PEP_util_enz. IPR002192. PPDK_PEP-bd. IPR015813. Pyrv/PenolPyrv_Kinase. [Graphical view]
Pfam	PF00391. PEP-utilizers. 2 hits. PF02896. PEP-utilizers_C. 1 hit. PF01326. PPDK_N. 1 hit. [Graphical view]
PRINTS	PR01736. PHPHTRNFRASE.
SMART	SM00306. HintN. 1 hit. [Graphical view]
SUPFAM	SSF52009. PEP_mobile. 2 hits. SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
TIGRFAMs	TIGR01443. intein_Cterm. 1 hit. TIGR01445. intein_Nterm. 1 hit.
PROSITE	PS50818. INTEIN_C_TER. 1 hit. PS50819. INTEIN_ENDONUCLEASE. 1 hit. PS50817. INTEIN_N_TER. 1 hit. PS00742. PEP_ENZYMES_2. 1 hit. PS00370. PEP_ENZYMES_PHOS_SITE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PPSA_METJA

Accession

Primary (citable) accession number: Q57962

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Intein-containing proteins

List of intein-containing protein entries

Methanococcus jannaschii

Methanococcus jannaschii: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Cleaved into the following chain:

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents