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Protein

Probable phosphoenolpyruvate synthase

Gene

ppsA

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate.By similarity

Catalytic activityi

ATP + pyruvate + H2O = AMP + phosphoenolpyruvate + phosphate.

Cofactori

Mg2+By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei824 – 8241Tele-phosphohistidine intermediateBy similarity
Binding sitei917 – 9171SubstrateBy similarity
Binding sitei964 – 9641SubstrateBy similarity
Metal bindingi1061 – 10611MagnesiumBy similarity
Binding sitei1061 – 10611SubstrateBy similarity
Binding sitei1083 – 10831Substrate; via carbonyl oxygenBy similarity
Binding sitei1084 – 10841Substrate; via amide nitrogenBy similarity
Binding sitei1085 – 10851SubstrateBy similarity
Metal bindingi1086 – 10861MagnesiumBy similarity
Binding sitei1086 – 10861Substrate; via amide nitrogenBy similarity
Active sitei1133 – 11331Proton donorBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. endonuclease activity Source: InterPro
  3. metal ion binding Source: UniProtKB-KW
  4. pyruvate, water dikinase activity Source: UniProtKB-EC

GO - Biological processi

  1. gluconeogenesis Source: UniProtKB-UniPathway
  2. intein-mediated protein splicing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable phosphoenolpyruvate synthase (EC:2.7.9.2)
Short name:
PEP synthase
Alternative name(s):
Pyruvate, water dikinase
Cleaved into the following chain:
Alternative name(s):
Mja pepA intein
Gene namesi
Name:ppsA
Ordered Locus Names:MJ0542
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
ProteomesiUP000000805: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 410410Probable phosphoenolpyruvate synthase, 1st partSequence AnalysisPRO_0000023556Add
BLAST
Chaini411 – 822412Mja pep inteinSequence AnalysisPRO_0000023557Add
BLAST
Chaini823 – 1188366Probable phosphoenolpyruvate synthase, 2nd partSequence AnalysisPRO_0000023558Add
BLAST

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.Curated

Keywords - PTMi

Autocatalytic cleavage, Phosphoprotein, Protein splicing

Interactioni

Protein-protein interaction databases

STRINGi243232.MJ0542.

Structurei

3D structure databases

ProteinModelPortaliQ57962.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini536 – 670135DOD-type homing endonucleasePROSITE-ProRule annotationAdd
BLAST

Domaini

The N-terminal domain contains the ATP/Pi binding site, the central domain the pyrophosphate/phosphate carrier histidine, and the C-terminal domain the pyruvate binding site.By similarity

Sequence similaritiesi

Belongs to the PEP-utilizing enzyme family.Curated
Contains 1 DOD-type homing endonuclease domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0574.
InParanoidiQ57962.
KOiK01007.
OMAiRPITTIG.

Family and domain databases

Gene3Di3.10.28.10. 1 hit.
3.20.20.60. 1 hit.
3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.50.30.10. 2 hits.
InterProiIPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR028992. Hedgehog/Intein_dom.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR008279. PEP-util_enz_mobile_dom.
IPR000121. PEP_util_C.
IPR023151. PEP_util_CS.
IPR006318. PEP_util_enz.
IPR002192. PPDK_PEP-bd.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PfamiPF00391. PEP-utilizers. 2 hits.
PF02896. PEP-utilizers_C. 1 hit.
PF01326. PPDK_N. 1 hit.
[Graphical view]
PRINTSiPR01736. PHPHTRNFRASE.
SMARTiSM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF51294. SSF51294. 2 hits.
SSF51621. SSF51621. 1 hit.
SSF52009. SSF52009. 1 hit.
SSF55608. SSF55608. 1 hit.
TIGRFAMsiTIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
PROSITEiPS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
PS00742. PEP_ENZYMES_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q57962-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLIIQNTKGD SMKFIAWLDE LSNKDVDIAG GKGASLGEMW NAGLPVPPAF
60 70 80 90 100
VVTADAYRHF IKETGLMDKI REILSGLDVN DTDALTNASK KIRKLIEEAE
110 120 130 140 150
MPEDLRLAII EAYNKLCEMC GEDEVTVAVR SSATAEDLPE ASFAGQQDTY
160 170 180 190 200
LNIKGAENVV KYVQKCFSSL FTPRAIFYRE QQGFDHFKVA LAAVVQKLVN
210 220 230 240 250
AEKAGVMFTV NPISENYDEL VIEAAWGLGE GVVSGSVSPD TYIVNKKTLE
260 270 280 290 300
IVDKHIARKE TMFVKDEKGE TKVVEVPDDM KEKQVLSDDE IKELAKIGLN
310 320 330 340 350
IEKHYGKPMD VEWAYEKGKF YMLQARPITT LKKGKKEKKA KEEDIEAKIL
360 370 380 390 400
LKGIGASPGI ATGVVKIIHD VSEIDKVKEG DILVTEMTTP DMVPAMKKAA
410 420 430 440 450
AIVTDEGGLT CIEGDAKILT DRGFLKMKEV YKLVKNGEKL KVLGLNAETL
460 470 480 490 500
KTEWKEIIDA QKREARRYEI GVYRKNKNTK DTIKITPDHK FPVFVNGELS
510 520 530 540 550
KVQLCDIIDN NLSVLSIDYI PMIEEKYESL AEVMYLGGAV LSDGHIVRRN
560 570 580 590 600
GKPIRVRFTQ KDTEEKKDFI EKVKGDVKLI GGNFIEISNR NNVIEYQTSR
610 620 630 640 650
KIPSEILGFI EVNINTIPLY ATKDEIADLI AGFVDGDGCL SGKRRVEIYQ
660 670 680 690 700
NSSHIKKIEG LIVGLYRLGI IPRLRYKRSS TATIYFNNNL ETILQRTRRI
710 720 730 740 750
KLDKLKEFKK PVEDKKLIDI SQILPELKEF DYKGYLYKTY KEKLFIGINK
760 770 780 790 800
LEEYLSKIDK DGIERIKQKI KLLKESDIYS IRIKKVGEDY GEVYNITVKA
810 820 830 840 850
ENEFNHNYVV WTKHYTPIVV FNCHAAIVSR ELGTPCVVGT KKATKVLKDG
860 870 880 890 900
MIVTVDGEKG IVYEGEIKKV EEKEKKQEVV VQQAPIITAT EVKVNVSMPE
910 920 930 940 950
VAERAAATGA DGVGLLRAEH MILGLGKHPR KILEEEGEEA LIEALMEGIR
960 970 980 990 1000
KVADAFYPRP VTYRTLDAPT DEFRGLEGGE NEPIEHNPML GWRGIRRDLD
1010 1020 1030 1040 1050
EVDILKCELK AIKRLREEGY KNIEIMIPLV THPDEVRRVK EIMREVGLEP
1060 1070 1080 1090 1100
CKDIPFGIMV ETPAAALIIE DFIKEGINFV SLGTNDLTQY TIAIDRNNEL
1110 1120 1130 1140 1150
VSKYYKEDHP AVLKLVEHVI KTCKKHGIKT SICGQAGSRP HIVEKLVEWG
1160 1170 1180
IDSVSANIDA VETIRRVVAR TEQKVILNYI RKSYVERE
Length:1,188
Mass (Da):134,012
Last modified:November 1, 1996 - v1
Checksum:i5547E2E2A8C46AEC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB98534.1.
PIRiF64367.
RefSeqiNP_247521.1. NC_000909.1.
WP_010870046.1. NC_000909.1.

Genome annotation databases

EnsemblBacteriaiAAB98534; AAB98534; MJ_0542.
GeneIDi1451407.
KEGGimja:MJ_0542.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB98534.1.
PIRiF64367.
RefSeqiNP_247521.1. NC_000909.1.
WP_010870046.1. NC_000909.1.

3D structure databases

ProteinModelPortaliQ57962.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243232.MJ0542.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB98534; AAB98534; MJ_0542.
GeneIDi1451407.
KEGGimja:MJ_0542.

Phylogenomic databases

eggNOGiCOG0574.
InParanoidiQ57962.
KOiK01007.
OMAiRPITTIG.

Enzyme and pathway databases

UniPathwayiUPA00138.

Family and domain databases

Gene3Di3.10.28.10. 1 hit.
3.20.20.60. 1 hit.
3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.50.30.10. 2 hits.
InterProiIPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR028992. Hedgehog/Intein_dom.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR008279. PEP-util_enz_mobile_dom.
IPR000121. PEP_util_C.
IPR023151. PEP_util_CS.
IPR006318. PEP_util_enz.
IPR002192. PPDK_PEP-bd.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PfamiPF00391. PEP-utilizers. 2 hits.
PF02896. PEP-utilizers_C. 1 hit.
PF01326. PPDK_N. 1 hit.
[Graphical view]
PRINTSiPR01736. PHPHTRNFRASE.
SMARTiSM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF51294. SSF51294. 2 hits.
SSF51621. SSF51621. 1 hit.
SSF52009. SSF52009. 1 hit.
SSF55608. SSF55608. 1 hit.
TIGRFAMsiTIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
PROSITEiPS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
PS00742. PEP_ENZYMES_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Entry informationi

Entry nameiPPSA_METJA
AccessioniPrimary (citable) accession number: Q57962
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: February 4, 2015
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The reaction takes place in three steps, mediated by a phosphocarrier histidine residue located on the surface of the central domain. The two first partial reactions are catalyzed at an active site located on the N-terminal domain, and the third partial reaction is catalyzed at an active site located on the C-terminal domain. For catalytic turnover, the central domain swivels from the concave surface of the N-terminal domain to that of the C-terminal domain (By similarity).By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.