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Protein

tRNA (guanine(6)-N2)-methyltransferase

Gene

trm14

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the methylation of the guanosine nucleotide at position 6 (m2G6) in tRNA(Cys).1 Publication

Catalytic activityi

S-adenosyl-L-methionine + guanine6 in tRNA = S-adenosyl-L-homocysteine + N(2)-methylguanine6 in tRNA.1 Publication

GO - Molecular functioni

  • tRNA (guanine) methyltransferase activity Source: UniProtKB
  • tRNA binding Source: GO_Central

GO - Biological processi

  • tRNA methylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BRENDAi2.1.1.256. 3260.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine(6)-N2)-methyltransferase (EC:2.1.1.256)
Alternative name(s):
tRNA m2G6-methyltransferase
Gene namesi
Name:trm14
Ordered Locus Names:MJ0438
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
Proteomesi
  • UP000000805 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001404801 – 381tRNA (guanine(6)-N2)-methyltransferaseAdd BLAST381

Interactioni

Protein-protein interaction databases

STRINGi243232.MJ_0438.

Structurei

3D structure databases

ProteinModelPortaliQ57880.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini43 – 157THUMPPROSITE-ProRule annotationAdd BLAST115

Sequence similaritiesi

Belongs to the methyltransferase superfamily.Curated
Contains 1 THUMP domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiarCOG00048. Archaea.
COG0116. LUCA.
InParanoidiQ57880.
KOiK16318.
OMAiDHPAHLK.
PhylomeDBiQ57880.

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
InterProiIPR002052. DNA_methylase_N6_adenine_CS.
IPR000241. RNA_methylase_dom.
IPR029063. SAM-dependent_MTases.
IPR004114. THUMP_dom.
[Graphical view]
PfamiPF02926. THUMP. 1 hit.
PF01170. UPF0020. 1 hit.
[Graphical view]
SMARTiSM00981. THUMP. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 2 hits.
PROSITEiPS00092. N6_MTASE. 1 hit.
PS51165. THUMP. 1 hit.
PS01261. UPF0020. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q57880-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYYVTLSPG LEKISKNEIE SFGGKIKEIR ENKGRIFFSG DLKLIPKINY
60 70 80 90 100
LSRTIERMNI LLHREEIPNI ALDDIYKRVY NIDWTEWIKE NQSFAIRPLR
110 120 130 140 150
AGEHNFTSID IGRVAGEAVI KSYQRDKNIR LKVNLDEPDV IVRVEVIFDE
160 170 180 190 200
LIVGIDTTGD IALDKRGYRV FNHPAHLNAT IASSLVYLSD WKDDEMLLDP
210 220 230 240 250
MCGSGTIPIE GALMKRNIPP GKFRENKYGF KFIDIFGYEL LDKIKKEIVE
260 270 280 290 300
NKNIYKIIGL DKNQKYLDGA KDNAKNAEVL DTIEFICGDA TKLHEKFNES
310 320 330 340 350
DVIIANPPYG IRIGSKRSVK KLYDEFLSSA KEIMHGSSRL IVITAEDKMF
360 370 380
KDAIAKNNFE VKEEFNVMFG GLMTRVFYLT L
Length:381
Mass (Da):43,738
Last modified:November 1, 1996 - v1
Checksum:i9BB972D94479444D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB98426.1.
PIRiF64354.
RefSeqiWP_010869937.1. NC_000909.1.

Genome annotation databases

EnsemblBacteriaiAAB98426; AAB98426; MJ_0438.
GeneIDi1451298.
KEGGimja:MJ_0438.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB98426.1.
PIRiF64354.
RefSeqiWP_010869937.1. NC_000909.1.

3D structure databases

ProteinModelPortaliQ57880.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243232.MJ_0438.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB98426; AAB98426; MJ_0438.
GeneIDi1451298.
KEGGimja:MJ_0438.

Phylogenomic databases

eggNOGiarCOG00048. Archaea.
COG0116. LUCA.
InParanoidiQ57880.
KOiK16318.
OMAiDHPAHLK.
PhylomeDBiQ57880.

Enzyme and pathway databases

BRENDAi2.1.1.256. 3260.

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
InterProiIPR002052. DNA_methylase_N6_adenine_CS.
IPR000241. RNA_methylase_dom.
IPR029063. SAM-dependent_MTases.
IPR004114. THUMP_dom.
[Graphical view]
PfamiPF02926. THUMP. 1 hit.
PF01170. UPF0020. 1 hit.
[Graphical view]
SMARTiSM00981. THUMP. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 2 hits.
PROSITEiPS00092. N6_MTASE. 1 hit.
PS51165. THUMP. 1 hit.
PS01261. UPF0020. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRM14_METJA
AccessioniPrimary (citable) accession number: Q57880
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: October 5, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.