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Protein

tRNA (guanine(6)-N2)-methyltransferase

Gene

trm14

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the methylation of the guanosine nucleotide at position 6 (m2G6) in tRNA(Cys).1 Publication

Catalytic activityi

S-adenosyl-L-methionine + guanine6 in tRNA = S-adenosyl-L-homocysteine + N2-methylguanine6 in tRNA.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei261S-adenosyl-L-methionineBy similarity1
Binding sitei306S-adenosyl-L-methionineBy similarity1

GO - Molecular functioni

  • RNA binding Source: UniProtKB-KW
  • tRNA (guanine) methyltransferase activity Source: UniProtKB

GO - Biological processi

  • tRNA methylation Source: UniProtKB

Keywordsi

Molecular functionMethyltransferase, RNA-binding, Transferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BRENDAi2.1.1.256. 3260.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine(6)-N2)-methyltransferaseCurated (EC:2.1.1.2561 Publication)
Alternative name(s):
tRNA m2G6-methyltransferaseCurated
Gene namesi
Name:trm141 Publication
Ordered Locus Names:MJ0438
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
Proteomesi
  • UP000000805 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001404801 – 381tRNA (guanine(6)-N2)-methyltransferaseAdd BLAST381

Interactioni

Protein-protein interaction databases

STRINGi243232.MJ_0438.

Structurei

3D structure databases

ProteinModelPortaliQ57880.
SMRiQ57880.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini43 – 157THUMPPROSITE-ProRule annotationAdd BLAST115

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni173 – 177S-adenosyl-L-methionine bindingBy similarity5
Regioni204 – 206S-adenosyl-L-methionine bindingBy similarity3
Regioni289 – 290S-adenosyl-L-methionine bindingBy similarity2

Sequence similaritiesi

Belongs to the methyltransferase superfamily.Curated

Phylogenomic databases

eggNOGiarCOG00048. Archaea.
COG0116. LUCA.
InParanoidiQ57880.
KOiK16318.
OMAiIECRLFR.
OrthoDBiPOG093Z05IZ.
PhylomeDBiQ57880.

Family and domain databases

InterProiView protein in InterPro
IPR002052. DNA_methylase_N6_adenine_CS.
IPR000241. RNA_methylase_dom.
IPR029063. SAM-dependent_MTases.
IPR004114. THUMP_dom.
PfamiView protein in Pfam
PF02926. THUMP. 1 hit.
PF01170. UPF0020. 1 hit.
SMARTiView protein in SMART
SM00981. THUMP. 1 hit.
SUPFAMiSSF53335. SSF53335. 2 hits.
PROSITEiView protein in PROSITE
PS00092. N6_MTASE. 1 hit.
PS51165. THUMP. 1 hit.
PS01261. UPF0020. 1 hit.

Sequencei

Sequence statusi: Complete.

Q57880-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYYVTLSPG LEKISKNEIE SFGGKIKEIR ENKGRIFFSG DLKLIPKINY
60 70 80 90 100
LSRTIERMNI LLHREEIPNI ALDDIYKRVY NIDWTEWIKE NQSFAIRPLR
110 120 130 140 150
AGEHNFTSID IGRVAGEAVI KSYQRDKNIR LKVNLDEPDV IVRVEVIFDE
160 170 180 190 200
LIVGIDTTGD IALDKRGYRV FNHPAHLNAT IASSLVYLSD WKDDEMLLDP
210 220 230 240 250
MCGSGTIPIE GALMKRNIPP GKFRENKYGF KFIDIFGYEL LDKIKKEIVE
260 270 280 290 300
NKNIYKIIGL DKNQKYLDGA KDNAKNAEVL DTIEFICGDA TKLHEKFNES
310 320 330 340 350
DVIIANPPYG IRIGSKRSVK KLYDEFLSSA KEIMHGSSRL IVITAEDKMF
360 370 380
KDAIAKNNFE VKEEFNVMFG GLMTRVFYLT L
Length:381
Mass (Da):43,738
Last modified:November 1, 1996 - v1
Checksum:i9BB972D94479444D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB98426.1.
PIRiF64354.
RefSeqiWP_010869937.1. NC_000909.1.

Genome annotation databases

EnsemblBacteriaiAAB98426; AAB98426; MJ_0438.
GeneIDi1451298.
KEGGimja:MJ_0438.

Similar proteinsi

Entry informationi

Entry nameiTRM14_METJA
AccessioniPrimary (citable) accession number: Q57880
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: September 27, 2017
This is version 104 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families