Q57840 (RPOE1_METJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase subunit E'
EC=2.7.7.6

Gene names

Name:	rpoE1
Ordered Locus Names:	MJ0397

Organism

Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)

Taxonomic identifier

243232 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	187 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Sequence similarities	Contains 1 S1 motif domain.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Molecular function	Nucleotidyltransferase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 187

187

DNA-directed RNA polymerase subunit E'

PRO_0000074036

Regions

Domain

82 – 166

85

S1 motif

Secondary structure

1

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187

Helix Strand Turn

Details...

Beta strand

2 – 13

12

Helix

15 – 17

3

Helix

22 – 34

13

Turn

40 – 42

3

Beta strand

43 – 54

12

Beta strand

65 – 77

13

Beta strand

84 – 93

10

Beta strand

96 – 100

5

Beta strand

102 – 109

8

Helix

110 – 112

3

Beta strand

118 – 120

3

Beta strand

127 – 129

3

Turn

130 – 132

3

Beta strand

141 – 150

10

Beta strand

160 – 164

5

Helix

173 – 183

11

Sequences

	Sequence		Length	Mass (Da)
	Q57840 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: C0E4EA3C006D69DB	FASTA	187	21,216
	10 20 30 40 50 60 MYKILEIADV VKVPPEEFGK DLKETVKKIL MEKYEGRLDK DVGFVLSIVD VKDIGEGKVV 70 80 90 100 110 120 HGDGSAYHPV VFETLVYIPE MYELIEGEVV DVVEFGSFVR LGPLDGLIHV SQIMDDYVSY 130 140 150 160 170 180 DPKREAIIGK ETGKVLEIGD YVRARIVAIS LKAERKRGSK IALTMRQPYL GKLEWIEEEK AKKQNQE

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

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Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L77117 Genomic DNA. Translation: AAB98387.1.

PIR

E64349.

RefSeq

NP_247371.1. NC_000909.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1GO3	X-ray	1.75	E/M	1-187	[»]

ProteinModelPortal

Q57840.

SMR

Q57840. Positions 1-181.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q57840. 1 interaction.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

1451254.

GenomeReviews

Gene locus MJ0397 in contig L77117_GR.

KEGG

mja:MJ_0397.

NMPDR

fig|243232.1.peg.408.

TIGR

MJ0397.

Phylogenomic databases

HOGENOM

HBG512792.

OMA

PRDSKIG.

ProtClustDB

PRK08563.

Enzyme and pathway databases

BioCyc

MJAN243232:MJ_0397-MONOMER.

Family and domain databases

InterPro

IPR004519. DNA-dir_RNA_polE.
IPR012340. NA-bd_OB-fold.
IPR016027. NA-bd_OB-fold-like.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR022967. RNA-binding_domain_S1.
IPR005576. RNA_pol_Rpb7_N.
[Graphical view]

Gene3D

G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
G3DSA:3.30.1490.120. RNA_pol_Rpb7_N. 1 hit.

KO

K03049.

Pfam

PF03876. RNA_pol_Rpb7_N. 1 hit.
PF00575. S1. 1 hit.
[Graphical view]

SMART

SM00316. S1. 1 hit.
[Graphical view]

SUPFAM

SSF50249. Nucleic_acid_OB. 1 hit.
SSF88798. RNA_pol_Rpb7_N. 1 hit.

TIGRFAMs

TIGR00448. RpoE. 1 hit.

PROSITE

PS50126. S1. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

RPOE1_METJA

Accession

Primary (citable) accession number: Q57840

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	November 16, 2011
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program