Q57839 (RPOE2_METJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase subunit E''
EC=2.7.7.6

Gene names

Name:	rpoE2
Ordered Locus Names:	MJ0396

Organism

Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)

Taxonomic identifier

243232 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	59 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Nucleotidyltransferase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 59

59

DNA-directed RNA polymerase subunit E''

PRO_0000074037

Regions

Zinc finger

4 – 19

16

C4-type Potential

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

59

Helix Strand Turn

Details...

Beta strand

2 – 4

3

Turn

5 – 7

3

Beta strand

9 – 15

7

Turn

17 – 19

3

Beta strand

22 – 25

4

Beta strand

30 – 34

5

Turn

36 – 38

3

Helix

40 – 44

5

Beta strand

53 – 57

5

Sequences

	Sequence		Length	Mass (Da)
	Q57839 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: CFC635FF7C1B0919	FASTA	59	6,578
	10 20 30 40 50 MRACLKCKYL TNDEICPICH SPTSENWIGL LIVINPEKSE IAKKAGIDIK GKYALSVKE

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

+

Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L77117 Genomic DNA. Translation: AAB98386.1.

PIR

D64349.

RefSeq

NP_247370.1. NC_000909.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3LPE	X-ray	1.90	B/D/F/H	1-59	[»]

ProteinModelPortal

Q57839.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-46332N.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

1451253.

GenomeReviews

Gene locus MJ0396 in contig L77117_GR.

KEGG

mja:MJ_0396.

NMPDR

fig|243232.1.peg.407.

TIGR

MJ0396.

Phylogenomic databases

HOGENOM

HBG297509.

OMA

TSENWIG.

ProtClustDB

CLSK2749878.

Enzyme and pathway databases

BioCyc

MJAN243232:MJ_0396-MONOMER.

Family and domain databases

InterPro

IPR007178. DNA-dir_RNA_pol_RpoE2_arc.
IPR022800. Spt4/RpoE2_Znf.
[Graphical view]

KO

K03050.

Pfam

PF06093. Spt4. 1 hit.
[Graphical view]

ProDom

PD019197. DNA-dir_RNA_pol_RpoE2_arc. 1 hit.
[Graphical view] [Entries sharing at least one domain]

ProtoNet

Search...

Entry information

Entry name

RPOE2_METJA

Accession

Primary (citable) accession number: Q57839

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	November 16, 2011
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program