Q57836 (CBIT_METJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable cobalt-precorrin-6Y C(15)-methyltransferase [decarboxylating]
EC=2.1.1.-

Gene names

Name:	cbiT
Ordered Locus Names:	MJ0391

Organism

Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) [Reference proteome] [HAMAP]

Taxonomic identifier

243232 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus › Methanocaldococcus jannaschii

Protein attributes

Sequence length	183 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probably catalyzes the methylation of either C-15 or C-5 in cobalt-precorrin-6Y to form cobalt-precorrin-7W. Methylation of C-15 would probably be followed by a spontaneous decarboxylation of C-12 By similarity. HAMAP-Rule MF_00786
Pathway	Cofactor biosynthesis; adenosylcobalamin biosynthesis; cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route): step 8/10. HAMAP-Rule MF_00786
Sequence similarities	Belongs to the methyltransferase superfamily. Archaeal-type CbiT family.

Ontologies

Keywords
Biological process	Cobalamin biosynthesis
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cobalamin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway corrin biosynthetic process Inferred from electronic annotation. Source: HAMAP protein methylation Inferred from electronic annotation. Source: GOC
Molecular_function	cobalamin-transporting ATPase activity Inferred from electronic annotation. Source: HAMAP protein methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 183

183

Probable cobalt-precorrin-6Y C(15)-methyltransferase [decarboxylating] HAMAP-Rule MF_00786

PRO_0000134938

Regions

Region

43 – 47

5

S-adenosyl-L-methionine binding By similarity

Sites

Binding site

19

1

S-adenosyl-L-methionine By similarity

Binding site

64

1

S-adenosyl-L-methionine By similarity

Binding site

92

1

S-adenosyl-L-methionine; via amide nitrogen By similarity

Secondary structure

1

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183

Helix Strand Turn

Details...

Helix

21 – 31

11

Beta strand

38 – 43

6

Helix

48 – 54

7

Beta strand

57 – 64

8

Helix

67 – 79

13

Beta strand

84 – 90

7

Helix

92 – 95

4

Helix

96 – 98

3

Beta strand

102 – 106

5

Helix

112 – 121

10

Beta strand

126 – 132

7

Helix

134 – 146

13

Beta strand

150 – 164

15

Beta strand

167 – 172

6

Beta strand

176 – 182

7

Sequences

	Sequence		Length	Mass (Da)
	Q57836 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: CB47419BB8781340	FASTA	183	20,502
	10 20 30 40 50 60 MKYMIPDEEF IRREGVPITK EEIRAVSIGK LNLNKDDVVV DVGCGSGGMT VEIAKRCKFV 70 80 90 100 110 120 YAIDYLDGAI EVTKQNLAKF NIKNCQIIKG RAEDVLDKLE FNKAFIGGTK NIEKIIEILD 130 140 150 160 170 180 KKKINHIVAN TIVLENAAKI INEFESRGYN VDAVNVFISY AKKIPSGHMF LAKNPITIIK AVR

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L77117 Genomic DNA. Translation: AAB98376.1.

PIR

G64348.

RefSeq

NP_247365.1. NC_000909.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2YXD	X-ray	2.30	A/B	1-183	[»]

ProteinModelPortal

Q57836.

SMR

Q57836. Positions 4-183.

ModBase

Search...

Protein-protein interaction databases

STRING

243232.MJ0391.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAB98376; AAB98376; MJ_0391.

GeneID

1451248.

KEGG

mja:MJ_0391.

Phylogenomic databases

eggNOG

COG2242.

KO

K02191.

OMA

IRAVSIG.

ProtClustDB

CLSK876165.

Enzyme and pathway databases

UniPathway

UPA00148; UER00229.

Family and domain databases

HAMAP

MF_00786. CbiT.

InterPro

IPR014008. Cbl_synth_MTase_CbiT.
IPR023475. Cbl_synth_MTase_CbiT_arc-type.
IPR025714. Methyltranfer_dom.
[Graphical view]

Pfam

PF13847. Methyltransf_31. 1 hit.
[Graphical view]

TIGRFAMs

TIGR02469. CbiT. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

Q57836.

Entry information

Entry name

CBIT_METJA

Accession

Primary (citable) accession number: Q57836

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program