Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Transcription elongation factor Spt5

Gene

spt5

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Stimulates transcription elongation.UniRule annotation1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciMJAN243232:G1GKE-398-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription elongation factor Spt5UniRule annotation
Gene namesi
Name:spt5UniRule annotation
Ordered Locus Names:MJ0372
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
Proteomesi
  • UP000000805 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi4A → R: Abrogates binding to RNAP. Decreases elongation activity. 1 Publication1
Mutagenesisi42Y → A: Abrogates binding to RNAP. Decreases elongation activity. 1 Publication1
Mutagenesisi44L → A: Can still bind RNAP, but with a decreased affinity. Does not affect elongation activity. 1 Publication1
Mutagenesisi44L → R: Abrogates binding to RNAP. Decreases elongation activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001139781 – 147Transcription elongation factor Spt5Add BLAST147

Proteomic databases

PRIDEiQ57818

Interactioni

Subunit structurei

Heterodimer composed of Spt4 and Spt5. Interacts with RNA polymerase (RNAP). Forms a homodimer in solution.UniRule annotation2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
spt4Q578393EBI-15739382,EBI-15739363

Protein-protein interaction databases

DIPiDIP-46333N
IntActiQ57818, 1 interactor
STRINGi243232.MJ_0372

Structurei

Secondary structure

1147
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 7Combined sources6
Helixi12 – 25Combined sources14
Beta strandi30 – 35Combined sources6
Beta strandi42 – 49Combined sources8
Helixi50 – 57Combined sources8
Beta strandi63 – 66Combined sources4
Helixi73 – 80Combined sources8
Beta strandi94 – 97Combined sources4
Turni101 – 104Combined sources4
Beta strandi106 – 113Combined sources8
Turni114 – 117Combined sources4
Beta strandi118 – 124Combined sources7
Beta strandi132 – 135Combined sources4
Helixi136 – 138Combined sources3
Beta strandi139 – 143Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3EWGX-ray2.04A1-83[»]
3LPEX-ray1.90A/C/E/G1-82[»]
4ZN1X-ray2.80A1-147[»]
4ZN3X-ray2.30A1-147[»]
ProteinModelPortaliQ57818
SMRiQ57818
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ57818

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini91 – 122KOWUniRule annotationAdd BLAST32

Domaini

Composed of only a NusG N-terminal (NGN) domain and a KOW domain, similar to bacterial NusG. The NGN domain is the effector domain of the complex that mediates the interaction with RNAP and is essential for elongation activity.2 Publications

Sequence similaritiesi

Belongs to the archaeal Spt5 family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01920 Archaea
COG0250 LUCA
InParanoidiQ57818
KOiK02601
OMAiFSEIEHF
OrthoDBiPOG093Z0DP6
PhylomeDBiQ57818

Family and domain databases

Gene3Di2.30.30.30, 1 hit
3.30.70.940, 1 hit
HAMAPiMF_00950 Spt5_arch, 1 hit
InterProiView protein in InterPro
IPR005824 KOW
IPR006645 NGN_dom
IPR036735 NGN_dom_sf
IPR014722 Rib_L2_dom2
IPR005825 Ribosomal_L24/26_CS
IPR011590 Spt5_arc
IPR005100 TF_Spt5_NGN-domain
IPR008991 Translation_prot_SH3-like_sf
PfamiView protein in Pfam
PF00467 KOW, 1 hit
PF03439 Spt5-NGN, 1 hit
SMARTiView protein in SMART
SM00739 KOW, 1 hit
SM00738 NGN, 1 hit
SUPFAMiSSF50104 SSF50104, 1 hit
TIGRFAMsiTIGR00405 KOW_elon_Spt5, 1 hit

Sequencei

Sequence statusi: Complete.

Q57818-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIFAVRTMVG QEKNIAGLMA SRAEKEQLDV YSILASESLK GYVLVEAETK
60 70 80 90 100
GDVEELIKGM PRVRGIVPGT IAIEEIEPLL TPKKIIENIE KGDVVEIIAG
110 120 130 140
PFKGERAKVI RVDKHKEEVT LELENAAVPI PITLPVEGVK IVSKHKD
Length:147
Mass (Da):16,136
Last modified:November 1, 1996 - v1
Checksum:iA8D917571BF5F215
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA Translation: AAB98361.1
PIRiD64346
RefSeqiWP_010869871.1, NC_000909.1

Genome annotation databases

EnsemblBacteriaiAAB98361; AAB98361; MJ_0372
GeneIDi1451229
KEGGimja:MJ_0372

Similar proteinsi

Entry informationi

Entry nameiSPT5_METJA
AccessioniPrimary (citable) accession number: Q57818
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 110 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health