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Protein

Transcription elongation factor Spt5

Gene

spt5

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Stimulates transcription elongation.UniRule annotation1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription elongation factor Spt5UniRule annotation
Gene namesi
Name:spt5UniRule annotation
Ordered Locus Names:MJ0372
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
Proteomesi
  • UP000000805 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi4 – 41A → R: Abrogates binding to RNAP. Decreases elongation activity. 1 Publication
Mutagenesisi42 – 421Y → A: Abrogates binding to RNAP. Decreases elongation activity. 1 Publication
Mutagenesisi44 – 441L → A: Can still bind RNAP, but with a decreased affinity. Does not affect elongation activity. 1 Publication
Mutagenesisi44 – 441L → R: Abrogates binding to RNAP. Decreases elongation activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 147147Transcription elongation factor Spt5PRO_0000113978Add
BLAST

Interactioni

Subunit structurei

Heterodimer composed of Spt4 and Spt5. Interacts with RNA polymerase (RNAP). Forms a homodimer in solution.UniRule annotation2 Publications

Protein-protein interaction databases

DIPiDIP-46333N.
STRINGi243232.MJ_0372.

Structurei

Secondary structure

1
147
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 76Combined sources
Helixi12 – 2514Combined sources
Beta strandi30 – 356Combined sources
Beta strandi42 – 498Combined sources
Helixi50 – 578Combined sources
Beta strandi63 – 664Combined sources
Helixi73 – 808Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3EWGX-ray2.04A1-83[»]
3LPEX-ray1.90A/C/E/G1-82[»]
4ZN1X-ray2.80A1-147[»]
4ZN3X-ray2.30A1-147[»]
ProteinModelPortaliQ57818.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ57818.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini91 – 12232KOWUniRule annotationAdd
BLAST

Domaini

Composed of only a NusG N-terminal (NGN) domain and a KOW domain, similar to bacterial NusG. The NGN domain is the effector domain of the complex that mediates the interaction with RNAP and is essential for elongation activity.2 Publications

Sequence similaritiesi

Belongs to the archaeal Spt5 family.UniRule annotation
Contains 1 KOW domain.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01920. Archaea.
COG0250. LUCA.
InParanoidiQ57818.
KOiK02601.
OMAiPIPITIR.
PhylomeDBiQ57818.

Family and domain databases

Gene3Di2.30.30.30. 1 hit.
3.30.70.940. 1 hit.
HAMAPiMF_00950. Spt5_arch.
InterProiIPR005824. KOW.
IPR006645. NGN_dom.
IPR014722. Rib_L2_dom2.
IPR005825. Ribosomal_L24/26_CS.
IPR011590. Spt5_arc.
IPR005100. TF_Spt5_NGN-domain.
IPR008991. Translation_prot_SH3-like.
[Graphical view]
PfamiPF00467. KOW. 1 hit.
PF03439. Spt5-NGN. 1 hit.
[Graphical view]
SMARTiSM00739. KOW. 1 hit.
SM00738. NGN. 1 hit.
[Graphical view]
SUPFAMiSSF50104. SSF50104. 1 hit.
TIGRFAMsiTIGR00405. KOW_elon_Spt5. 1 hit.

Sequencei

Sequence statusi: Complete.

Q57818-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIFAVRTMVG QEKNIAGLMA SRAEKEQLDV YSILASESLK GYVLVEAETK
60 70 80 90 100
GDVEELIKGM PRVRGIVPGT IAIEEIEPLL TPKKIIENIE KGDVVEIIAG
110 120 130 140
PFKGERAKVI RVDKHKEEVT LELENAAVPI PITLPVEGVK IVSKHKD
Length:147
Mass (Da):16,136
Last modified:November 1, 1996 - v1
Checksum:iA8D917571BF5F215
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB98361.1.
PIRiD64346.

Genome annotation databases

EnsemblBacteriaiAAB98361; AAB98361; MJ_0372.
KEGGimja:MJ_0372.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB98361.1.
PIRiD64346.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3EWGX-ray2.04A1-83[»]
3LPEX-ray1.90A/C/E/G1-82[»]
4ZN1X-ray2.80A1-147[»]
4ZN3X-ray2.30A1-147[»]
ProteinModelPortaliQ57818.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-46333N.
STRINGi243232.MJ_0372.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB98361; AAB98361; MJ_0372.
KEGGimja:MJ_0372.

Phylogenomic databases

eggNOGiarCOG01920. Archaea.
COG0250. LUCA.
InParanoidiQ57818.
KOiK02601.
OMAiPIPITIR.
PhylomeDBiQ57818.

Miscellaneous databases

EvolutionaryTraceiQ57818.

Family and domain databases

Gene3Di2.30.30.30. 1 hit.
3.30.70.940. 1 hit.
HAMAPiMF_00950. Spt5_arch.
InterProiIPR005824. KOW.
IPR006645. NGN_dom.
IPR014722. Rib_L2_dom2.
IPR005825. Ribosomal_L24/26_CS.
IPR011590. Spt5_arc.
IPR005100. TF_Spt5_NGN-domain.
IPR008991. Translation_prot_SH3-like.
[Graphical view]
PfamiPF00467. KOW. 1 hit.
PF03439. Spt5-NGN. 1 hit.
[Graphical view]
SMARTiSM00739. KOW. 1 hit.
SM00738. NGN. 1 hit.
[Graphical view]
SUPFAMiSSF50104. SSF50104. 1 hit.
TIGRFAMsiTIGR00405. KOW_elon_Spt5. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.
  2. "Crystal structure of NusG N-terminal (NGN) domain from Methanocaldococcus jannaschii and its interaction with rpoE''."
    Zhou H., Liu Q., Gao Y., Teng M., Niu L.
    Proteins 76:787-793(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.04 ANGSTROMS) OF 1-83, DOMAIN, SUBUNIT.
  3. "Spt4/5 stimulates transcription elongation through the RNA polymerase clamp coiled-coil motif."
    Hirtreiter A., Damsma G.E., Cheung A.C., Klose D., Grohmann D., Vojnic E., Martin A.C., Cramer P., Werner F.
    Nucleic Acids Res. 38:4040-4051(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) OF 1-82, FUNCTION, SUBUNIT, DOMAIN, MUTAGENESIS OF ALA-4; TYR-42 AND LEU-44.

Entry informationi

Entry nameiSPT5_METJA
AccessioniPrimary (citable) accession number: Q57818
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.