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Protein

Type 2 DNA topoisomerase 6 subunit A

Gene

top6A

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Relaxes both positive and negative superturns and exhibits a strong decatenase activity.UniRule annotation1 Publication

Catalytic activityi

ATP-dependent breakage, passage and rejoining of double-stranded DNA.UniRule annotation1 Publication

Cofactori

Mg2+UniRule annotation1 PublicationNote: Mg2+ is directly coordinated by Glu-197 and Asp-249 as well as indirectly coordinated through 2 water molecules by Asp-251 (PubMed:10545127).1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei103 – 1031NucleophileBy similarity
Metal bindingi197 – 1971Magnesium1 Publication
Metal bindingi249 – 2491Magnesium1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

ATP-binding, DNA-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Type 2 DNA topoisomerase 6 subunit AUniRule annotation (EC:5.99.1.3UniRule annotation1 Publication)
Alternative name(s):
Type II DNA topoisomerase VI subunit AUniRule annotation
Gene namesi
Name:top6AUniRule annotation
Ordered Locus Names:MJ0369
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
Proteomesi
  • UP000000805 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi197 – 1971E → A: Reduces affinity for DNA. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 369369Type 2 DNA topoisomerase 6 subunit APRO_0000145449Add
BLAST

Interactioni

Subunit structurei

Homodimer (PubMed:10545127). Heterotetramer of two Top6A and two Top6B chains.UniRule annotation1 Publication

Protein-protein interaction databases

STRINGi243232.MJ_0369.

Structurei

Secondary structure

1
369
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi73 – 9119Combined sources
Helixi98 – 1047Combined sources
Helixi105 – 1073Combined sources
Helixi109 – 1113Combined sources
Helixi116 – 13015Combined sources
Helixi134 – 1374Combined sources
Beta strandi147 – 1515Combined sources
Beta strandi153 – 1586Combined sources
Beta strandi161 – 1666Combined sources
Beta strandi173 – 1764Combined sources
Beta strandi185 – 1873Combined sources
Beta strandi191 – 1977Combined sources
Helixi199 – 2079Combined sources
Helixi210 – 2134Combined sources
Beta strandi216 – 2205Combined sources
Helixi227 – 24014Combined sources
Beta strandi244 – 2474Combined sources
Helixi252 – 2565Combined sources
Helixi258 – 2636Combined sources
Beta strandi282 – 2865Combined sources
Helixi288 – 2936Combined sources
Helixi303 – 31513Combined sources
Helixi317 – 3204Combined sources
Helixi323 – 33513Combined sources
Beta strandi337 – 3393Combined sources
Helixi341 – 3477Combined sources
Helixi351 – 3544Combined sources
Helixi356 – 3627Combined sources
Helixi364 – 3663Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1D3YX-ray2.00A/B69-369[»]
ProteinModelPortaliQ57815.
SMRiQ57815. Positions 71-369.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ57815.

Family & Domainsi

Sequence similaritiesi

Belongs to the TOP6A family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04143. Archaea.
COG1697. LUCA.
InParanoidiQ57815.
KOiK03166.
OMAiWEEQIEL.
PhylomeDBiQ57815.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
HAMAPiMF_00132. Top6A. 1 hit.
InterProiIPR002815. Spo11/TopoVI_A.
IPR013049. Spo11/TopoVI_A_N.
IPR004085. TopoVI_A.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR10848. PTHR10848. 1 hit.
PfamiPF04406. TP6A_N. 1 hit.
[Graphical view]
PRINTSiPR01550. TOP6AFAMILY.
PR01552. TPISMRASE6A.
SUPFAMiSSF56726. SSF56726. 1 hit.

Sequencei

Sequence statusi: Complete.

Q57815-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKLPAISKK PREIAKQKII ELAKKMYEDL MKGKRPKITM PIRSLSNAMF
60 70 80 90 100
DKEKGSFTLV GKEKARTLTV NQAKIFAQTT KMLEFAKQLL ETDDFSTLRE
110 120 130 140 150
AYYVSKNWGE ARFDDQQASN NVIEDLEAAL GVLREHLGFI PEEDGSSVVG
160 170 180 190 200
PLKIIEETPE GELVVDCTKL GTGAYNIPND VTKLNLETDA DFILAIETSG
210 220 230 240 250
MFARLNAERF WDKHNCILVS LKGVPARATR RFIKRLHEEH DLPVLVFTDG
260 270 280 290 300
DPYGYLNIYR TLKVGSGKAI HLADKLSIPA ARLIGVTPQD IIDYDLPTHP
310 320 330 340 350
LKEQDIKRIK DGLKNDDFVR SFPEWQKALK QMLDMGVRAE QQSLAKYGLK
360
YVVNTYLPEK IKDESTWLP
Length:369
Mass (Da):41,829
Last modified:November 1, 1996 - v1
Checksum:i7F75282B3E547834
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB98358.1.
PIRiA64346.

Genome annotation databases

EnsemblBacteriaiAAB98358; AAB98358; MJ_0369.
KEGGimja:MJ_0369.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB98358.1.
PIRiA64346.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1D3YX-ray2.00A/B69-369[»]
ProteinModelPortaliQ57815.
SMRiQ57815. Positions 71-369.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243232.MJ_0369.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB98358; AAB98358; MJ_0369.
KEGGimja:MJ_0369.

Phylogenomic databases

eggNOGiarCOG04143. Archaea.
COG1697. LUCA.
InParanoidiQ57815.
KOiK03166.
OMAiWEEQIEL.
PhylomeDBiQ57815.

Miscellaneous databases

EvolutionaryTraceiQ57815.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
HAMAPiMF_00132. Top6A. 1 hit.
InterProiIPR002815. Spo11/TopoVI_A.
IPR013049. Spo11/TopoVI_A_N.
IPR004085. TopoVI_A.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR10848. PTHR10848. 1 hit.
PfamiPF04406. TP6A_N. 1 hit.
[Graphical view]
PRINTSiPR01550. TOP6AFAMILY.
PR01552. TPISMRASE6A.
SUPFAMiSSF56726. SSF56726. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTOP6A_METJA
AccessioniPrimary (citable) accession number: Q57815
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: January 20, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.