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Protein

Type 2 DNA topoisomerase 6 subunit A

Gene

top6A

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Relaxes both positive and negative superturns and exhibits a strong decatenase activity.UniRule annotation1 Publication

Catalytic activityi

ATP-dependent breakage, passage and rejoining of double-stranded DNA.UniRule annotation1 Publication

Cofactori

Mg2+UniRule annotation1 PublicationNote: Mg2+ is directly coordinated by Glu-197 and Asp-249 as well as indirectly coordinated through 2 water molecules by Asp-251 (PubMed:10545127).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei103NucleophileBy similarity1
Metal bindingi197Magnesium1 Publication1
Metal bindingi249Magnesium1 Publication1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

ATP-binding, DNA-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Type 2 DNA topoisomerase 6 subunit AUniRule annotation (EC:5.99.1.3UniRule annotation1 Publication)
Alternative name(s):
Type II DNA topoisomerase VI subunit AUniRule annotation
Gene namesi
Name:top6AUniRule annotation
Ordered Locus Names:MJ0369
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
Proteomesi
  • UP000000805 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi197E → A: Reduces affinity for DNA. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001454491 – 369Type 2 DNA topoisomerase 6 subunit AAdd BLAST369

Interactioni

Subunit structurei

Homodimer (PubMed:10545127). Heterotetramer of two Top6A and two Top6B chains.UniRule annotation1 Publication

Protein-protein interaction databases

STRINGi243232.MJ_0369.

Structurei

Secondary structure

1369
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi73 – 91Combined sources19
Helixi98 – 104Combined sources7
Helixi105 – 107Combined sources3
Helixi109 – 111Combined sources3
Helixi116 – 130Combined sources15
Helixi134 – 137Combined sources4
Beta strandi147 – 151Combined sources5
Beta strandi153 – 158Combined sources6
Beta strandi161 – 166Combined sources6
Beta strandi173 – 176Combined sources4
Beta strandi185 – 187Combined sources3
Beta strandi191 – 197Combined sources7
Helixi199 – 207Combined sources9
Helixi210 – 213Combined sources4
Beta strandi216 – 220Combined sources5
Helixi227 – 240Combined sources14
Beta strandi244 – 247Combined sources4
Helixi252 – 256Combined sources5
Helixi258 – 263Combined sources6
Beta strandi282 – 286Combined sources5
Helixi288 – 293Combined sources6
Helixi303 – 315Combined sources13
Helixi317 – 320Combined sources4
Helixi323 – 335Combined sources13
Beta strandi337 – 339Combined sources3
Helixi341 – 347Combined sources7
Helixi351 – 354Combined sources4
Helixi356 – 362Combined sources7
Helixi364 – 366Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1D3YX-ray2.00A/B69-369[»]
ProteinModelPortaliQ57815.
SMRiQ57815.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ57815.

Family & Domainsi

Sequence similaritiesi

Belongs to the TOP6A family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04143. Archaea.
COG1697. LUCA.
InParanoidiQ57815.
KOiK03166.
OMAiWEEQIEL.
PhylomeDBiQ57815.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
HAMAPiMF_00132. Top6A. 1 hit.
InterProiIPR002815. Spo11/TopoVI_A.
IPR013049. Spo11/TopoVI_A_N.
IPR004085. TopoVI_A.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR10848. PTHR10848. 1 hit.
PfamiPF04406. TP6A_N. 1 hit.
[Graphical view]
PRINTSiPR01550. TOP6AFAMILY.
PR01552. TPISMRASE6A.
SUPFAMiSSF56726. SSF56726. 1 hit.

Sequencei

Sequence statusi: Complete.

Q57815-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKLPAISKK PREIAKQKII ELAKKMYEDL MKGKRPKITM PIRSLSNAMF
60 70 80 90 100
DKEKGSFTLV GKEKARTLTV NQAKIFAQTT KMLEFAKQLL ETDDFSTLRE
110 120 130 140 150
AYYVSKNWGE ARFDDQQASN NVIEDLEAAL GVLREHLGFI PEEDGSSVVG
160 170 180 190 200
PLKIIEETPE GELVVDCTKL GTGAYNIPND VTKLNLETDA DFILAIETSG
210 220 230 240 250
MFARLNAERF WDKHNCILVS LKGVPARATR RFIKRLHEEH DLPVLVFTDG
260 270 280 290 300
DPYGYLNIYR TLKVGSGKAI HLADKLSIPA ARLIGVTPQD IIDYDLPTHP
310 320 330 340 350
LKEQDIKRIK DGLKNDDFVR SFPEWQKALK QMLDMGVRAE QQSLAKYGLK
360
YVVNTYLPEK IKDESTWLP
Length:369
Mass (Da):41,829
Last modified:November 1, 1996 - v1
Checksum:i7F75282B3E547834
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB98358.1.
PIRiA64346.
RefSeqiWP_010869868.1. NC_000909.1.

Genome annotation databases

EnsemblBacteriaiAAB98358; AAB98358; MJ_0369.
GeneIDi1451226.
KEGGimja:MJ_0369.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB98358.1.
PIRiA64346.
RefSeqiWP_010869868.1. NC_000909.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1D3YX-ray2.00A/B69-369[»]
ProteinModelPortaliQ57815.
SMRiQ57815.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243232.MJ_0369.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB98358; AAB98358; MJ_0369.
GeneIDi1451226.
KEGGimja:MJ_0369.

Phylogenomic databases

eggNOGiarCOG04143. Archaea.
COG1697. LUCA.
InParanoidiQ57815.
KOiK03166.
OMAiWEEQIEL.
PhylomeDBiQ57815.

Miscellaneous databases

EvolutionaryTraceiQ57815.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
HAMAPiMF_00132. Top6A. 1 hit.
InterProiIPR002815. Spo11/TopoVI_A.
IPR013049. Spo11/TopoVI_A_N.
IPR004085. TopoVI_A.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR10848. PTHR10848. 1 hit.
PfamiPF04406. TP6A_N. 1 hit.
[Graphical view]
PRINTSiPR01550. TOP6AFAMILY.
PR01552. TPISMRASE6A.
SUPFAMiSSF56726. SSF56726. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTOP6A_METJA
AccessioniPrimary (citable) accession number: Q57815
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.