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Protein

Blue-light-activated histidine kinase

Gene

BruAb2_0636

Organism
Brucella abortus biovar 1 (strain 9-941)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Photosensitive kinase that is involved in increased bacterial virulence upon exposure to light. Once ejected from an infected animal host, sunlight acts as an environmental signal that increases the virulence of the bacterium, preparing it for infection of the next host. This photoreceptor protein is directly related to the bacterium's survival and replication within host macrophages (By similarity).By similarity

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Photoreceptor protein, Receptor, Transferase

Keywords - Biological processi

Sensory transduction, Virulence

Keywords - Ligandi

ATP-binding, Chromophore, Flavoprotein, FMN, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Blue-light-activated histidine kinase (EC:2.7.13.3)
Gene namesi
Ordered Locus Names:BruAb2_0636
OrganismiBrucella abortus biovar 1 (strain 9-941)
Taxonomic identifieri262698 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
Proteomesi
  • UP000000540 Componenti: Chromosome II

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003612831 – 489Blue-light-activated histidine kinaseAdd BLAST489

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei69S-4a-FMN cysteineBy similarity1
Modified residuei288Phosphohistidine; by autocatalysisBy similarity1

Post-translational modificationi

FMN binds covalently to cysteine after exposure to blue light and this bond is spontaneously broken in the dark.By similarity

Keywords - PTMi

Phosphoprotein

Structurei

3D structure databases

ProteinModelPortaliQ577Y7.
SMRiQ577Y7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini19 – 93PASPROSITE-ProRule annotationAdd BLAST75
Domaini93 – 147PAC 1PROSITE-ProRule annotationAdd BLAST55
Domaini232 – 281PAC 2PROSITE-ProRule annotationAdd BLAST50

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni254 – 336HWE histidine kinase domainAdd BLAST83

Sequence similaritiesi

Contains 2 PAC (PAS-associated C-terminal) domains.PROSITE-ProRule annotation
Contains 1 PAS (PER-ARNT-SIM) domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000032444.

Family and domain databases

InterProiIPR001610. PAC.
IPR000014. PAS.
IPR000700. PAS-assoc_C.
IPR013655. PAS_fold_3.
IPR011102. Sig_transdc_His_kinase_HWE.
[Graphical view]
PfamiPF07536. HWE_HK. 1 hit.
PF08447. PAS_3. 1 hit.
PF13426. PAS_9. 1 hit.
[Graphical view]
SMARTiSM00911. HWE_HK. 1 hit.
SM00086. PAC. 2 hits.
SM00091. PAS. 2 hits.
[Graphical view]
SUPFAMiSSF55785. SSF55785. 2 hits.
TIGRFAMsiTIGR00229. sensory_box. 2 hits.
PROSITEiPS50113. PAC. 2 hits.
PS50112. PAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q577Y7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIDLRPFIP FGRGALSQAT DPFRAAVEFT LMPMLITNPH LPDNPIVFAN
60 70 80 90 100
PAFLKLTGYE ADEVMGRNCR FLQGHGTDPA HVRAIKSAIA AEKPIDIDII
110 120 130 140 150
NYKKSGEAFW NRLHISPVHN ANGRLQHFVS SQLDVTLELS RLVELEKERK
160 170 180 190 200
TLSIETARSK DQLDYIVEVA NIGFWTREFY SGKMTCSAEC RRIYGFTPDE
210 220 230 240 250
PVHFDTILDL VVLEDRMTVV QKAHQAVTGE PYSIEYRIVT RLGETRWLET
260 270 280 290 300
RAKALTGENP LVLGIVQDVT ERKKAEANKA LVSREIAHRF KNSMAMVQSI
310 320 330 340 350
ANQTLRNTYD PEQANRLFSE RLRALSQAHD MLLKENWAGA TIQQICATAL
360 370 380 390 400
APFNSTFANR IHMSGPHLLV SDRVTVALSL AFYELATNAV KYGALSNEKG
410 420 430 440 450
VINITWAIME DKGEKKFHMR WAESRGPEVM QPARRGFGQR LLHSVLAEEL
460 470 480
KAKCDVEFAA SGLLIDVLAP ITPEVFPGMG HNVPEQRIA
Length:489
Mass (Da):54,874
Last modified:January 20, 2009 - v2
Checksum:i6F2A04467FB26C5B
GO

Sequence cautioni

The sequence AAX76047 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017224 Genomic DNA. Translation: AAX76047.1. Different initiation.
RefSeqiWP_002971240.1. NC_006933.1.

Genome annotation databases

EnsemblBacteriaiAAX76047; AAX76047; BruAb2_0636.
GeneIDi3827969.
KEGGibmb:BruAb2_0636.
PATRICi17827551. VBIBruAbo15061_2950.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017224 Genomic DNA. Translation: AAX76047.1. Different initiation.
RefSeqiWP_002971240.1. NC_006933.1.

3D structure databases

ProteinModelPortaliQ577Y7.
SMRiQ577Y7.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAX76047; AAX76047; BruAb2_0636.
GeneIDi3827969.
KEGGibmb:BruAb2_0636.
PATRICi17827551. VBIBruAbo15061_2950.

Phylogenomic databases

HOGENOMiHOG000032444.

Family and domain databases

InterProiIPR001610. PAC.
IPR000014. PAS.
IPR000700. PAS-assoc_C.
IPR013655. PAS_fold_3.
IPR011102. Sig_transdc_His_kinase_HWE.
[Graphical view]
PfamiPF07536. HWE_HK. 1 hit.
PF08447. PAS_3. 1 hit.
PF13426. PAS_9. 1 hit.
[Graphical view]
SMARTiSM00911. HWE_HK. 1 hit.
SM00086. PAC. 2 hits.
SM00091. PAS. 2 hits.
[Graphical view]
SUPFAMiSSF55785. SSF55785. 2 hits.
TIGRFAMsiTIGR00229. sensory_box. 2 hits.
PROSITEiPS50113. PAC. 2 hits.
PS50112. PAS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLOVHK_BRUAB
AccessioniPrimary (citable) accession number: Q577Y7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: January 20, 2009
Last modified: November 2, 2016
This is version 68 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Brucella abortus strain 9-941
    Brucella abortus (strain 9-941): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.