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Q577Y2

- GLMU_BRUAB

UniProt

Q577Y2 - GLMU_BRUAB

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Protein

Bifunctional protein GlmU

Gene

glmU

Organism
Brucella abortus biovar 1 (strain 9-941)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc). The C-terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N-acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5-triphosphate), a reaction catalyzed by the N-terminal domain.UniRule annotation

Catalytic activityi

Acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + N-acetyl-alpha-D-glucosamine 1-phosphate.UniRule annotation
UTP + N-acetyl-alpha-D-glucosamine 1-phosphate = diphosphate + UDP-N-acetyl-alpha-D-glucosamine.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg(2+) ion per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei25 – 251UDP-GlcNAcUniRule annotation
Binding sitei78 – 781UDP-GlcNAcUniRule annotation
Metal bindingi108 – 1081MagnesiumUniRule annotation
Binding sitei144 – 1441UDP-GlcNAc; via amide nitrogenUniRule annotation
Binding sitei158 – 1581UDP-GlcNAcUniRule annotation
Binding sitei173 – 1731UDP-GlcNAcUniRule annotation
Metal bindingi230 – 2301MagnesiumUniRule annotation
Binding sitei230 – 2301UDP-GlcNAcUniRule annotation
Binding sitei319 – 3191Acetyl-CoA; amide nitrogenUniRule annotation
Binding sitei337 – 3371Acetyl-CoAUniRule annotation
Active sitei349 – 3491Proton acceptorUniRule annotation
Binding sitei352 – 3521Acetyl-CoAUniRule annotation
Binding sitei363 – 3631Acetyl-CoAUniRule annotation
Binding sitei391 – 3911Acetyl-CoAUniRule annotation
Binding sitei409 – 4091Acetyl-CoA; via amide nitrogenUniRule annotation
Binding sitei426 – 4261Acetyl-CoAUniRule annotation

GO - Molecular functioni

  1. glucosamine-1-phosphate N-acetyltransferase activity Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. UDP-N-acetylglucosamine diphosphorylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. cell morphogenesis Source: UniProtKB-HAMAP
  2. cell wall organization Source: UniProtKB-KW
  3. lipid A biosynthetic process Source: UniProtKB-UniPathway
  4. lipopolysaccharide biosynthetic process Source: InterPro
  5. peptidoglycan biosynthetic process Source: UniProtKB-HAMAP
  6. regulation of cell shape Source: UniProtKB-KW
  7. UDP-N-acetylglucosamine biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciBABO262698:GJC2-2856-MONOMER.
UniPathwayiUPA00113; UER00532.
UPA00113; UER00533.
UPA00973.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein GlmUUniRule annotation
Including the following 2 domains:
UDP-N-acetylglucosamine pyrophosphorylaseUniRule annotation (EC:2.7.7.23UniRule annotation)
Alternative name(s):
N-acetylglucosamine-1-phosphate uridyltransferaseUniRule annotation
Glucosamine-1-phosphate N-acetyltransferaseUniRule annotation (EC:2.3.1.157UniRule annotation)
Gene namesi
Name:glmUUniRule annotation
Ordered Locus Names:BruAb2_0641
OrganismiBrucella abortus biovar 1 (strain 9-941)
Taxonomic identifieri262698 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000000540: Chromosome II

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 454454Bifunctional protein GlmUPRO_0000233744Add
BLAST

Interactioni

Subunit structurei

Homotrimer.UniRule annotation

Protein-protein interaction databases

STRINGi262698.BruAb2_0641.

Structurei

3D structure databases

ProteinModelPortaliQ577Y2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 232232PyrophosphorylaseUniRule annotationAdd
BLAST
Regioni11 – 144UDP-GlcNAc bindingUniRule annotation
Regioni83 – 842UDP-GlcNAc bindingUniRule annotation
Regioni233 – 25321LinkerUniRule annotationAdd
BLAST
Regioni254 – 454201N-acetyltransferaseUniRule annotationAdd
BLAST
Regioni372 – 3732Acetyl-CoA bindingUniRule annotation

Sequence similaritiesi

In the N-terminal section; belongs to the N-acetylglucosamine-1-phosphate uridyltransferase family.UniRule annotation
In the C-terminal section; belongs to the transferase hexapeptide repeat family.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG1207.
HOGENOMiHOG000283476.
KOiK04042.
OMAiIGERAYI.
OrthoDBiEOG6Z6FQZ.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
HAMAPiMF_01631. GlmU.
InterProiIPR005882. Bifunctional_GlmU.
IPR001451. Hexapep_transf.
IPR018357. Hexapep_transf_CS.
IPR025877. MobA-like_NTP_Trfase_dom.
IPR029044. Nucleotide-diphossugar_trans.
IPR011004. Trimer_LpxA-like.
[Graphical view]
PfamiPF00132. Hexapep. 4 hits.
PF12804. NTP_transf_3. 1 hit.
[Graphical view]
SUPFAMiSSF51161. SSF51161. 1 hit.
SSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR01173. glmU. 1 hit.
PROSITEiPS00101. HEXAPEP_TRANSFERASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q577Y2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTDRTCLSIV LAAGEGTRMK SNLPKVLHRV AGLPLVCHVV NAVRGTGKSD
60 70 80 90 100
VALVVGRGAE DVRSAVEKIA GPVSAFEQKE RLGTAHAVLA AHEAIARGYD
110 120 130 140 150
DLLIVFGDTP LIEAQSLLAA RERLAQGADL VVIGFRPASP HGYGRLIEEG
160 170 180 190 200
GQLVAIIEEK EATDEQKKIG FCNGGLMALR GQHALALLDA VGNDNAKGEY
210 220 230 240 250
YLTDIVAIAH GKGLNVTAIE VPVDNVIGIN NRAELAEAET IWQNRKRREL
260 270 280 290 300
MLSGVTLIAP ETVFFSYDTV IEPDVVIEPN VFFGPSVHVA SGALIHSFSH
310 320 330 340 350
LEGAQVGEKA EIGPFARLRP GADLAEKSKV GNFCEVKNAK VGKGAKINHL
360 370 380 390 400
AYIGDAVIGA SSNIGAGTIT CNYDGYNKFK TIIGDNAFIG SNSSLVAPVE
410 420 430 440 450
IGDNAYIASG SVITADVPAD ALALGRARQE TKEGRAKILR EKYAAIKAAK

SVSK
Length:454
Mass (Da):47,886
Last modified:May 10, 2005 - v1
Checksum:i98D0D7E9AA0EAF2C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017224 Genomic DNA. Translation: AAX76052.1.
RefSeqiYP_223413.1. NC_006933.1.

Genome annotation databases

EnsemblBacteriaiAAX76052; AAX76052; BruAb2_0641.
GeneIDi3342275.
KEGGibmb:BruAb2_0641.
PATRICi17827563. VBIBruAbo15061_2955.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017224 Genomic DNA. Translation: AAX76052.1 .
RefSeqi YP_223413.1. NC_006933.1.

3D structure databases

ProteinModelPortali Q577Y2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 262698.BruAb2_0641.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAX76052 ; AAX76052 ; BruAb2_0641 .
GeneIDi 3342275.
KEGGi bmb:BruAb2_0641.
PATRICi 17827563. VBIBruAbo15061_2955.

Phylogenomic databases

eggNOGi COG1207.
HOGENOMi HOG000283476.
KOi K04042.
OMAi IGERAYI.
OrthoDBi EOG6Z6FQZ.

Enzyme and pathway databases

UniPathwayi UPA00113 ; UER00532 .
UPA00113 ; UER00533 .
UPA00973 .
BioCyci BABO262698:GJC2-2856-MONOMER.

Family and domain databases

Gene3Di 3.90.550.10. 1 hit.
HAMAPi MF_01631. GlmU.
InterProi IPR005882. Bifunctional_GlmU.
IPR001451. Hexapep_transf.
IPR018357. Hexapep_transf_CS.
IPR025877. MobA-like_NTP_Trfase_dom.
IPR029044. Nucleotide-diphossugar_trans.
IPR011004. Trimer_LpxA-like.
[Graphical view ]
Pfami PF00132. Hexapep. 4 hits.
PF12804. NTP_transf_3. 1 hit.
[Graphical view ]
SUPFAMi SSF51161. SSF51161. 1 hit.
SSF53448. SSF53448. 1 hit.
TIGRFAMsi TIGR01173. glmU. 1 hit.
PROSITEi PS00101. HEXAPEP_TRANSFERASES. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis."
    Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z., Li L.-L., Kapur V., Alt D.P., Olsen S.C.
    J. Bacteriol. 187:2715-2726(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 9-941.

Entry informationi

Entry nameiGLMU_BRUAB
AccessioniPrimary (citable) accession number: Q577Y2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: May 10, 2005
Last modified: November 26, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. Brucella abortus strain 9-941
    Brucella abortus (strain 9-941): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3