Bifunctional protein glmU - Brucella abortus

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Reviewed, UniProtKB/Swiss-Prot Q577Y2 (GLMU_BRUAB)

Last modified February 9, 2010. Version 37. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Bifunctional protein glmU
Including the following 2 domains:
    1- Recommended name:
            UDP-N-acetylglucosamine pyrophosphorylase
              EC=2.7.7.23
        Alternative name(s):
            N-acetylglucosamine-1-phosphate uridyltransferase
    2- Recommended name:
            Glucosamine-1-phosphate N-acetyltransferase
              EC=2.3.1.157

Gene names

Name:	glmU
Ordered Locus Names:	BruAb2_0641

Organism

Brucella abortus [Complete proteome] [HAMAP]

Taxonomic identifier

235 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

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Protein attributes

Sequence length	454 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

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General annotation (Comments)

Function	Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-GlcNAc. Responsible for the acetylation of Glc-N-1-P to give GlcNAc-1-P and for the uridyl transfer from UTP to GlcNAc-1-P which produces UDP-GlcNAc By similarity. HAMAP MF_01631
Catalytic activity	Acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + N-acetyl-alpha-D-glucosamine 1-phosphate. HAMAP MF_01631 UTP + N-acetyl-alpha-D-glucosamine 1-phosphate = diphosphate + UDP-N-acetyl-D-glucosamine. HAMAP MF_01631
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_01631
Pathway	Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route II): step 2/2. HAMAP MF_01631 Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; UDP-N-acetyl-alpha-D-glucosamine from N-acetyl-alpha-D-glucosamine 1-phosphate: step 1/1. Bacterial outer membrane biogenesis; LPS lipid A biosynthesis. HAMAP MF_01631
Subcellular location	Cytoplasm By similarity HAMAP MF_01631.
Sequence similarities	In the N-terminal section; belongs to the N-acetylglucosamine-1-phosphate uridyltransferase family. In the C-terminal section; belongs to the transferase hexapeptide repeat family.

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Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Domain	Repeat
Ligand	Magnesium Metal-binding
Molecular function	Acyltransferase Nucleotidyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	cell morphogenesis Inferred from electronic annotation. Source: HAMAP cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: InterPro peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	UDP-N-acetylglucosamine diphosphorylase activity Inferred from electronic annotation. Source: HAMAP glucosamine-1-phosphate N-acetyltransferase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 454

454

Bifunctional protein glmU HAMAP MF_01631

PRO_0000233744

Regions

Region

1 – 232

232

Pyrophosphorylase By similarity

Region

11 – 14

Substrate binding By similarity

Region

83 – 84

Substrate binding By similarity

Region

233 – 253

Linker By similarity

Region

254 – 454

201

N-acetyltransferase By similarity

Sites

Active site

349

Proton acceptor By similarity

Metal binding

108

Magnesium By similarity

Metal binding

230

Magnesium By similarity

Binding site

Substrate By similarity

Binding site

144

Substrate By similarity

Binding site

158

Substrate By similarity

Binding site

173

Substrate By similarity

Binding site

373

Acetyl-CoA By similarity

Binding site

391

Acetyl-CoA By similarity

Binding site

426

Acetyl-CoA By similarity

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Sequences

Sequence

Length

Mass (Da)

Tools

Q577Y2-1 [UniParc].

Last modified May 10, 2005. Version 1.
Checksum: 98D0D7E9AA0EAF2C
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FASTA

454

47,886

        10         20         30         40         50         60 
MTDRTCLSIV LAAGEGTRMK SNLPKVLHRV AGLPLVCHVV NAVRGTGKSD VALVVGRGAE 

        70         80         90        100        110        120 
DVRSAVEKIA GPVSAFEQKE RLGTAHAVLA AHEAIARGYD DLLIVFGDTP LIEAQSLLAA 

       130        140        150        160        170        180 
RERLAQGADL VVIGFRPASP HGYGRLIEEG GQLVAIIEEK EATDEQKKIG FCNGGLMALR 

       190        200        210        220        230        240 
GQHALALLDA VGNDNAKGEY YLTDIVAIAH GKGLNVTAIE VPVDNVIGIN NRAELAEAET 

       250        260        270        280        290        300 
IWQNRKRREL MLSGVTLIAP ETVFFSYDTV IEPDVVIEPN VFFGPSVHVA SGALIHSFSH 

       310        320        330        340        350        360 
LEGAQVGEKA EIGPFARLRP GADLAEKSKV GNFCEVKNAK VGKGAKINHL AYIGDAVIGA 

       370        380        390        400        410        420 
SSNIGAGTIT CNYDGYNKFK TIIGDNAFIG SNSSLVAPVE IGDNAYIASG SVITADVPAD 

       430        440        450 
ALALGRARQE TKEGRAKILR EKYAAIKAAK SVSK

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References

[1]

"Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis."
Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z., Li L.-L., Kapur V., Alt D.P., Olsen S.C.
J. Bacteriol. 187:2715-2726(2005) [PubMed: 15805518] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 9-941 / Biovar 1.

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Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017224 Genomic DNA. Translation: AAX76052.1.
RefSeq	YP_223413.1.
3D structure databases
SMR	Q577Y2. Positions 7-438.
ModBase	Search...
Genome annotation databases
GeneID	3342275.
GenomeReviews	Gene locus BruAb2_0641 in contig AE017224_GR.
KEGG	bmb:BruAb2_0641.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG688195.
OMA	MERTCLA.
PhylomeDB	Q577Y2.
Enzyme and pathway databases
BioCyc	BABO262698:BRUAB2_0641-MONOMER.
BRENDA	2.3.1.157. 575. 2.7.7.23. 575.
Family and domain databases
HAMAP	MF_01631. GlmU. [Tree]
InterPro	IPR005882. Bifunctional_GlmU. IPR018357. Hexapep_transf_CS. IPR005835. NTP_transferase. IPR011004. Trimer_LpxA-like. [Graphical view]
Pfam	PF00483. NTP_transferase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01173. glmU. 1 hit.
PROSITE	PS00101. HEXAPEP_TRANSFERASES. 1 hit. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

GLMU_BRUAB

Accession

Primary (citable) accession number: Q577Y2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2006
Last sequence update:	May 10, 2005
Last modified:	February 9, 2010
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Brucella abortus strain 9-941

Brucella abortus (strain 9-941): entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents