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Q577P8

- BIOB_BRUAB

UniProt

Q577P8 - BIOB_BRUAB

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Protein

Biotin synthase

Gene
bioB, BruAb2_0730
Organism
Brucella abortus biovar 1 (strain 9-941)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi63 – 631Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi67 – 671Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi70 – 701Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi107 – 1071Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi138 – 1381Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi198 – 1981Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi270 – 2701Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciBABO262698:GJC2-2947-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:BruAb2_0730
OrganismiBrucella abortus biovar 1 (strain 9-941)
Taxonomic identifieri262698 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000000540: Chromosome II

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 328328Biotin synthaseUniRule annotationPRO_0000381249Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi262698.BruAb2_0730.

Structurei

3D structure databases

ProteinModelPortaliQ577P8.
SMRiQ577P8. Positions 15-322.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239957.
KOiK01012.
OMAiCATEDEA.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q577P8-1 [UniParc]FASTAAdd to Basket

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MPDRGGENGA SCSVGRWSAE EARAIYNLPF NDLLFRAHGL HRENFDPNRI    50
QLSKLLNIKT GGCPEDCGYC SQSASAENGL KASKLMEIET VLEEARKAKA 100
AGATRYCMGA AWRSPKDRDM PALTHMIESV KAMGLETCMT LGMLDSDKAE 150
KLADAGLDYY NHNIDTSERF YPAVITTRSF EDRLDTLANV RNAGIKVCSG 200
GILGLGEEAE DRIDMLVTLA NLPEPPESVP INMLIPMPGT RLAKAAPVDP 250
LEFVRVVALA RILMPKSHVR LTAGRTAMSD EMQALCFFAG ANSLFMGDTL 300
LTAANPGDDR DSSLLRRLGI QAETEQPA 328
Length:328
Mass (Da):35,653
Last modified:July 28, 2009 - v2
Checksum:iB3936726D01DAB70
GO

Sequence cautioni

The sequence AAX76136.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017224 Genomic DNA. Translation: AAX76136.1. Different initiation.
RefSeqiYP_223497.1. NC_006933.1.

Genome annotation databases

EnsemblBacteriaiAAX76136; AAX76136; BruAb2_0730.
GeneIDi3342165.
KEGGibmb:BruAb2_0730.
PATRICi17827742. VBIBruAbo15061_3043.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017224 Genomic DNA. Translation: AAX76136.1 . Different initiation.
RefSeqi YP_223497.1. NC_006933.1.

3D structure databases

ProteinModelPortali Q577P8.
SMRi Q577P8. Positions 15-322.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 262698.BruAb2_0730.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAX76136 ; AAX76136 ; BruAb2_0730 .
GeneIDi 3342165.
KEGGi bmb:BruAb2_0730.
PATRICi 17827742. VBIBruAbo15061_3043.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239957.
KOi K01012.
OMAi CATEDEA.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci BABO262698:GJC2-2947-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis."
    Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z., Li L.-L., Kapur V., Alt D.P., Olsen S.C.
    J. Bacteriol. 187:2715-2726(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 9-941.

Entry informationi

Entry nameiBIOB_BRUAB
AccessioniPrimary (citable) accession number: Q577P8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 28, 2009
Last modified: May 14, 2014
This is version 62 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Brucella abortus strain 9-941
    Brucella abortus (strain 9-941): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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