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Q577P6 (BIOD_BRUAB) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
ATP-dependent dethiobiotin synthetase BioD
EC=6.3.3.3
Alternative name(s):
DTB synthetase
Short name=DTBS
Dethiobiotin synthase
Gene names
Name:bioD
Ordered Locus Names:BruAb2_0732
OrganismBrucella abortus biovar 1 (strain 9-941) [Complete proteome] [HAMAP]
Taxonomic identifier262698 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length212 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA) to form an ureido ring By similarity. HAMAP MF_00336

Catalytic activity

ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336

Cofactor

Magnesium By similarity. HAMAP MF_00336

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336

Subcellular location

Cytoplasm By similarity HAMAP MF_00336.

Sequence similarities

Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
   Biological processBiotin biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processbiotin biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

dethiobiotin synthase activity

Inferred from electronic annotation. Source: EC

magnesium ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 212212ATP-dependent dethiobiotin synthetase BioD HAMAP MF_00336
PRO_0000302484

Regions

Nucleotide binding100 – 1034ATP By similarity
Nucleotide binding184 – 1863ATP By similarity

Sites

Metal binding171Magnesium 2 By similarity
Metal binding1001Magnesium 2 By similarity
Binding site371Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q577P6 [UniParc].

Last modified May 10, 2005. Version 1.
Checksum: 0FDA1023F9FF9921

FASTA21223,047
        10         20         30         40         50         60 
MNSRLIVTGT DTGIGKTVFS AALCHALGAV YWKPVQSGLE EETDSEIVAR LAQASPQRIL 

        70         80         90        100        110        120 
PEAWRLNTPA SPHLSARLDG VEIRPEEMHI PATSLPLVIE GAGGLLVPLN DKTLFADLFA 

       130        140        150        160        170        180 
IWRIPAILCA RAALGTINHT LLSLEAMRSR DIPVLGVAFI SEANEDTETT IAHLGRVKRL 

       190        200        210 
GRLPLLDDLS PEKLHHSFAR NFHIDDFAGV AR

« Hide

References

[1]"Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis."
Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z., Li L.-L., Kapur V., Alt D.P., Olsen S.C.
J. Bacteriol. 187:2715-2726(2005) [PubMed: 15805518] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 9-941.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE017224 Genomic DNA. Translation: AAX76138.1.
RefSeqYP_223499.1. NC_006933.1.

3D structure databases

HSSPHSSP built from PDB template 1DTS based on UniProtKB P13000.
ProteinModelPortalQ577P6.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3342163.
GenomeReviewsGene locus BruAb2_0732 in contig AE017224_GR.
KEGGbmb:BruAb2_0732.
PATRIC17827746. VBIBruAbo15061_3045.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG650065.
OMAWKPIQSG.
PhylomeDBQ577P6.
ProtClustDBPRK00090.

Enzyme and pathway databases

BioCycBABO262698:BRUAB2_0732-MONOMER.

Family and domain databases

HAMAPMF_00336. BioD.
[Tree]
InterProIPR004472. DTB_synth_BioD.
[Graphical view]
KOK01935.
PIRSFPIRSF006755. DTB_synth. 1 hit.
TIGRFAMsTIGR00347. BioD. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOD_BRUAB
AccessionPrimary (citable) accession number: Q577P6
Entry history
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: May 10, 2005
Last modified: January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Brucella abortus strain 9-941

Brucella abortus (strain 9-941): entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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