Q57770 (EF1A_METJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Elongation factor 1-alpha
Short name=EF-1-alpha

Alternative name(s):
Elongation factor Tu
Short name=EF-Tu

Gene names

Name:	tuf
Ordered Locus Names:	MJ0324

Organism

Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)

Taxonomic identifier

243232 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	428 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. HAMAP MF_00118_A
Subcellular location	Cytoplasm HAMAP MF_00118_A.
Sequence similarities	Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Nucleotide-binding
Molecular function	Elongation factor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: InterPro translation elongation factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 428	428	Elongation factor 1-alpha HAMAP MF_00118_A		PRO_0000090980
Regions
	Nucleotide binding	14 – 21	8	GTP By similarity
	Nucleotide binding	91 – 95	5	GTP By similarity
	Nucleotide binding	149 – 152	4	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q57770 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 0E375F776B8BA82E	FASTA	428	47,280
	10 20 30 40 50 60 MAKQKPVLNV AFIGHVDAGK STTVGRLLYD SGAIDPQLLE KLKREAQERG KAGFEFAYVM 70 80 90 100 110 120 DNLKEERERG VTIDVAHKKF ETQKYEVTIV DCPGHRDFIK NMITGASQAD AAVLVVDVND 130 140 150 160 170 180 AKTGIQPQTR EHMFLARTLG IKQIAVAINK MDTVNYSQEE YEKMKKMLSE QLLKVLGYNP 190 200 210 220 230 240 DQIDFIPTAS LKGDNVVKRS ENMPWYKGPT LVEALDKFQP PEKPTNLPLR IPIQDVYSIT 250 260 270 280 290 300 GVGTVPVGRV ETGILRPGDK VVFEPAGVSG EVKSIEMHHE QIPQAEPGDN IGFNVRGVSK 310 320 330 340 350 360 KDIKRGDVCG HPDNPPTVAE EFTAQIVVLQ HPTAITVGYT PVFHAHTAQV ACTFIELLKK 370 380 390 400 410 420 LDPRTGQVIE ENPQFLKTGD AAIVKIKPTK PMVIENVREI PQLGRFAIRD MGMTIAAGMA IDVKAKNK

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L77117 Genomic DNA. Translation: AAB98308.1.
PIR	D64340.
RefSeq	NP_247296.1. NC_000909.1.
3D structure databases
ProteinModelPortal	Q57770.
SMR	Q57770. Positions 5-424.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1451178.
GenomeReviews	Gene locus MJ0324 in contig L77117_GR.
KEGG	mja:MJ_0324.
NMPDR	fig|243232.1.peg.333.
TIGR	MJ0324.
Phylogenomic databases
HOGENOM	HBG307581.
OMA	FLKAGDA.
ProtClustDB	PRK12317.
Enzyme and pathway databases
BioCyc	MJAN243232:MJ_0324-MONOMER.
Family and domain databases
HAMAP	MF_00118_A. EF_Tu_A. [Tree]
InterPro	IPR000795. ProtSyn_GTP-bd. IPR005225. Small_GTP-bd_dom. IPR009001. Transl_elong_EF1A/Init_IF2_C. IPR004539. Transl_elong_EF1A_euk/arc. IPR004161. Transl_elong_EFTu/EF1A_2. IPR004160. Transl_elong_EFTu/EF1A_C. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
KO	K03231.
Pfam	PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. PF03143. GTP_EFTU_D3. 1 hit. [Graphical view]
PRINTS	PR00315. ELONGATNFCT.
SUPFAM	SSF50465. Elong_init_C. 1 hit. SSF50447. Translat_factor. 1 hit.
TIGRFAMs	TIGR00483. EF-1_alpha. 1 hit. TIGR00231. Small_GTP. 1 hit.
PROSITE	PS00301. EFACTOR_GTP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EF1A_METJA

Accession

Primary (citable) accession number: Q57770

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	November 16, 2011
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program