Q57729 (AMZA_METJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Archaemetzincin
EC=3.4.-.-

Gene names

Name:	amzA
Ordered Locus Names:	MJ0281

Organism

Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) [Reference proteome] [HAMAP]

Taxonomic identifier

243232 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus › Methanocaldococcus jannaschii

Protein attributes

Sequence length	179 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Probable zinc metalloprotease whose natural substrate is unknown By similarity. HAMAP-Rule MF_01842
Cofactor	Binds 2 zinc ions per subunit. One is catalytic, whereas the other seems to have a structural role By similarity.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the peptidase M54 family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: HAMAP
Molecular_function	metallopeptidase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 179	179	Archaemetzincin HAMAP-Rule MF_01842		PRO_0000159626
Sites
	Active site	129	1	Proton acceptor By similarity
	Metal binding	128	1	Zinc 1; catalytic; via tele nitrogen By similarity
	Metal binding	132	1	Zinc 1; catalytic; via tele nitrogen By similarity
	Metal binding	138	1	Zinc 1; catalytic; via tele nitrogen By similarity
	Metal binding	139	1	Zinc 2 By similarity
	Metal binding	144	1	Zinc 2 By similarity
	Metal binding	163	1	Zinc 2 By similarity
	Metal binding	166	1	Zinc 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q57729 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 895A7E935A757CE8	FASTA	179	20,514
	10 20 30 40 50 60 MVMIMRICIQ PVGDVNDEIL KFLKKKFGEV FGMCEILPKI DIPIYAYNFS RGQFNSTLIL 70 80 90 100 110 120 KSLPTVEDIV LGVTEVDIYA DNLNFVFGEA ELFGKRALIS LARLRPEFYG LPPNKDVLKI 130 140 150 160 170 RALKEAIHEI GHVLGLIHCE NKRCVMSFSN SIIDVDLKDW RYCKKCLKKL QDRGIYISI

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L77117 Genomic DNA. Translation: AAB98269.1.
PIR	B64335.
RefSeq	NP_247254.1. NC_000909.1.
3D structure databases
ProteinModelPortal	Q57729.
ModBase	Search...
Protein-protein interaction databases
STRING	243232.MJ0281.
Protein family/group databases
MEROPS	M54.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAB98269; AAB98269; MJ_0281.
GeneID	1451136.
KEGG	mja:MJ_0281.
Phylogenomic databases
eggNOG	COG1913.
KO	K06974.
OMA	CVISIAR.
ProtClustDB	CLSK2749866.
Family and domain databases
Gene3D	3.40.390.10. 1 hit.
HAMAP	MF_01842. Archaemetzincin.
InterPro	IPR024079. MetalloPept_cat_dom. IPR012962. Pept_M54_archaemetzincn. IPR012091. Pept_M54_archaemetzncn_arc/bac. [Graphical view]
Pfam	PF07998. Peptidase_M54. 1 hit. [Graphical view]
PIRSF	PIRSF005785. Zn-prot_arch. 1 hit.
PROSITE	PS00142. ZINC_PROTEASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMZA_METJA

Accession

Primary (citable) accession number: Q57729

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program