Q57725 (ILVB_METJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable acetolactate synthase large subunit
Short name=AHAS
EC=2.2.1.6

Alternative name(s):
Acetohydroxy-acid synthase large subunit
Short name=ALS

Gene names

Name:	ilvB
Ordered Locus Names:	MJ0277

Organism

Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)

Taxonomic identifier

243232 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	591 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2 pyruvate = 2-acetolactate + CO2.
Cofactor	Binds 1 magnesium ion per subunit By similarity. Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4. Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4.
Subunit structure	Dimer of large and small chains By similarity.
Sequence similarities	Belongs to the TPP enzyme family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	FAD Flavoprotein Magnesium Metal-binding Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	acetolactate synthase activity Inferred from electronic annotation. Source: EC flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: InterPro thiamine pyrophosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 591	591	Probable acetolactate synthase large subunit		PRO_0000090808
Regions
	Nucleotide binding	258 – 279	22	FAD By similarity
	Nucleotide binding	301 – 320	20	FAD By similarity
	Region	396 – 476	81	Thiamine pyrophosphate binding
Sites
	Metal binding	447	1	Magnesium By similarity
	Metal binding	474	1	Magnesium By similarity
	Binding site	47	1	Thiamine pyrophosphate By similarity
	Binding site	149	1	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q57725 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: DB398C926D9B3A9D	FASTA	591	64,493
	10 20 30 40 50 60 MKGAEAIIKA LEAEGVKIIF GYPGGAMLPF YDALYDSDLV HILTRHEQAA AHAADGFARA 70 80 90 100 110 120 SGEAGVCVST SGPGATNLVT GIATAYADSS PVIALTGQVP TKLIGNDAFQ EIDALGLFMP 130 140 150 160 170 180 ITKHNFQIKK PEEIPETFRA AFEIATTGRP GPVHIDLPKD VQDGEIDIEK YPIPAKVDLP 190 200 210 220 230 240 GYKPKTVGHP LQIKKAAKLI AESERPVILA GGGVIISGAS EELLRLAEFV KIPVCTTLMG 250 260 270 280 290 300 KGCFPEDHPL ALGMVGMHGT KAANYAVTEC DVLIAIGCRF SDRVTGDIRY FAPEAKIIHI 310 320 330 340 350 360 DIDPAEIGKN VRADIPIVGD AKNVLRDLLA ALIALEIKDK ETWLERIYEL KKLSIPMMDF 370 380 390 400 410 420 DDKPIKPQRF VKDLMEVLNE IDSKLKNTII TTDVGQNQMW MAHFFKTKMP RSFLASGGLG 430 440 450 460 470 480 TMGFGFPAAI GAKVAKPYAN VISITGDGGF LMNSQELATI SEYDIPVVIC IFDNRTLGMV 490 500 510 520 530 540 YQWQNLYYGQ RQSEVHLGES PDFVKLAESY GVKADRIISP DEIKEKLKEA ILSNEPYLLD 550 560 570 580 590 IVIDPAEALP MVPPGGRLTN IVQPIRVEPK IKKPQFDEIK KIRDMAAVKE F

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L77117 Genomic DNA. Translation: AAB98265.1.
PIR	F64334.
RefSeq	NP_247250.1. NC_000909.1.
3D structure databases
ProteinModelPortal	Q57725.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1451132.
GenomeReviews	Gene locus MJ0277 in contig L77117_GR.
KEGG	mja:MJ_0277.
NMPDR	fig|243232.1.peg.287.
TIGR	MJ0277.
Phylogenomic databases
HOGENOM	HBG323037.
OMA	WQNLYYG.
ProtClustDB	PRK06276.
Enzyme and pathway databases
BioCyc	MJAN243232:MJ_0277-MONOMER.
Family and domain databases
InterPro	IPR012846. Acetolactate_synth_lsu. IPR012000. Thiamin_PyroP_enz_cen_dom. IPR012001. Thiamin_PyroP_enz_TPP-bd_dom. IPR000399. TPP-bd_CS. IPR011766. TPP_enzyme-bd_C. [Graphical view]
KO	K01652.
PANTHER	PTHR18968:SF13. PTHR18968:SF13. 1 hit.
Pfam	PF02775. TPP_enzyme_C. 1 hit. PF00205. TPP_enzyme_M. 1 hit. PF02776. TPP_enzyme_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00118. Acolac_lg. 1 hit.
PROSITE	PS00187. TPP_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVB_METJA

Accession

Primary (citable) accession number: Q57725

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	November 16, 2011
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program