Reviewed, UniProtKB/Swiss-Prot Q57710 (IF2P_METJA)

Names and origin

Protein names

Recommended name:
    Probable translation initiation factor IF-2
Cleaved into the following chain:
    1- Recommended name:
            Mja infB intein
        Alternative name(s):
            Mja IF2 intein

Gene names

Name:	infB
Ordered Locus Names:	MJ0262

Organism

Methanocaldococcus jannaschii (Methanococcus jannaschii) [Complete proteome] [HAMAP]

Taxonomic identifier

2190 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	1155 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2 By similarity.
Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential.
Miscellaneous	The intein interrupts the GTP-binding site. HAMAP MF_00100
Sequence similarities	Belongs to the IF-2 family. Contains 1 DOD-type homing endonuclease domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	GTP-binding Nucleotide-binding
Molecular function	Initiation factor
PTM	Autocatalytic cleavage Protein splicing
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro translational initiation Inferred from electronic annotation. Source: HAMAP
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: HAMAP endonuclease activity Inferred from electronic annotation. Source: InterPro translation initiation factor activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 30	30	Probable translation initiation factor IF-2, 1st part Potential		PRO_0000014484
	Chain	31 – 576	546	Mja infB intein Potential		PRO_0000014485
	Chain	577 – 1155	579	Probable translation initiation factor IF-2, 2nd part Potential		PRO_0000014486
Regions
	Domain	237 – 367	131	DOD-type homing endonuclease
	Nucleotide binding	634 – 638	5	GTP By similarity
	Nucleotide binding	688 – 691	4	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q57710-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 6653FA355E7EE0D3	FASTA	1,155	132,299
	10 20 30 40 50 60 MAKKNTKKDN KNQNLRCPIV CVLGHVDHGK CLMPHEKVLT EYGEIKIEDL FKIGKEIVEK 70 80 90 100 110 120 DELKEIRKLN IKVHTLNENG EIKIINAPYV WKLKHKGKMI KVKLKNWHSI TTTPEHPFLT 130 140 150 160 170 180 NNGWIKAENI KKGMYVAIPR KIYGNEDFEK FIEFINSKIL TNELIVKVNE KDLKNVELPS 190 200 210 220 230 240 TKIYKKQKNV FRSEDIIEHN LNIEKISFSP RIHRCGKPQH YIKLPKSLNE WKAIFYFAGV 250 260 270 280 290 300 MFGDGCVDRI ANNDEEVFNK LKSLNNLGIE VERIKRKSSY EIIFKNGKNA LINLLKILFD 310 320 330 340 350 360 YPSEKKSHNI KIPQILYIAP KELVAEFIKG YFDADGYVNL RQNRIEVISA SKEFIEGLSI 370 380 390 400 410 420 LLLRFEITSK IYEIKKSYKE TKKKYYQLNI VGKRNLKNFK NIGFSIKYKE ENLNKIIEKS 430 440 450 460 470 480 RKSEKYPINK DMKRLRILFG MTRNEVNVSY YAKYENGKEI PSYEIVKKFL NSLKPKNLDK 490 500 510 520 530 540 KIKVLEGKER DVNYLKAFES DGLIENGRLT KLGREALNIW KNHEFGKENI DYMKSLIENI 550 560 570 580 590 600 AFVEVEDVEI IDYDGYVYDL TTETHNFIAN GIVVHNTTLL DKIRKTRVAK REAGGITQHI 610 620 630 640 650 660 GASEIPIDVI KRLCGDLLKM LKADLKIPGL LVIDTPGHEA FTSLRKRGGA LADIAILVVD 670 680 690 700 710 720 INEGFKPQTV EAVNILRQCK TPFVVAANKI DLIPGWNSKE GPFILNFNEK NQHPNALTEF 730 740 750 760 770 780 EIRLYENIIK PLNELGFDAD LYSRVQDVTK TVCIIPVSAV TGEGIPDLLM MVAGLAQKFL 790 800 810 820 830 840 EDRLKLNVEG YAKGTILEVK EEKGLGTTID AIIYDGIAKR GDYLVVGLPD DVLVTRVKAL 850 860 870 880 890 900 LKPKPLDEMR DPRDKFKPVN EVTAAAGVKI AAPELDKVIA GCPIRIVPKD KIEEAKEEVM 910 920 930 940 950 960 KEVEEAKIEV DDEGILIKAD TLGSLEALAN ELRKAGVKIK KAEVGDVTKK DVIEVASYKQ 970 980 990 1000 1010 1020 SNPLHGAIVA FNVKILPEAQ KEIEKYDIKV FLDNIIYKLV EDFTEWIKKE EERIKYGEFE 1030 1040 1050 1060 1070 1080 KLIKPAIIRI LPDCIFRQKD PAICGVEVLC GTLRVGAPLM REDGMQLGYV REIKDRGENV 1090 1100 1110 1120 1130 1140 KEAKAGKAVS IAIDGRVVLK RHVDEGDYMY VAVPESHVRE LYHKYMDRLR NDEKEALLRY 1150 MELMQKLTNN IFWGR

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L77117 Genomic DNA. Translation: AAB98248.1.
PIR	G64332.
RefSeq	NP_247234.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1451116.
GenomeReviews	Gene locus MJ0262 in contig L77117_GR.
KEGG	mja:MJ0262.
NMPDR	fig|243232.1.peg.271.
TIGR	MJ0262.
Phylogenomic databases
HOGENOM	Q57710.
OMA	TPEHPFL
Family and domain databases
HAMAP	MF_00100. [Tree]
InterPro	IPR015760. aIF-2. IPR003586. Hedgehog_hint_C. IPR003587. Hedgehog_hint_N. IPR006142. INTEIN. IPR004042. Intein_endonuc. IPR006141. Intein_splicing_site. IPR000795. ProtSyn_GTP_bd. IPR005225. Small_GTP_bd. IPR004544. TIF_aIF-2. IPR004161. Transl_elong_EFTu/EF1A_2. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
PANTHER	PTHR23115:SF41. aIF-2. 1 hit.
Pfam	PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. [Graphical view]
PRINTS	PR00379. INTEIN.
SMART	SM00305. HintC. 1 hit. SM00306. HintN. 1 hit. [Graphical view]
TIGRFAMs	TIGR00491. aIF-2. 1 hit. TIGR01443. intein_Cterm. 1 hit. TIGR01445. intein_Nterm. 1 hit. TIGR00231. small_GTP. 1 hit.
PROSITE	PS01176. IF2. False negative. PS50818. INTEIN_C_TER. 1 hit. PS50819. INTEIN_ENDONUCLEASE. 1 hit. PS50817. INTEIN_N_TER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IF2P_METJA

Accession

Primary (citable) accession number: Q57710

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	November 24, 2009
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)