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Protein

Probable translation initiation factor IF-2

Gene

infB

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2 (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi634 – 6385GTPBy similarity
Nucleotide bindingi688 – 6914GTPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Protein family/group databases

MEROPSiN10.004.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable translation initiation factor IF-2
Cleaved into the following chain:
Alternative name(s):
Mja IF2 intein
Gene namesi
Name:infB
Ordered Locus Names:MJ0262
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
ProteomesiUP000000805 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3030Probable translation initiation factor IF-2, 1st partSequence AnalysisPRO_0000014484Add
BLAST
Chaini31 – 576546Mja infB inteinSequence AnalysisPRO_0000014485Add
BLAST
Chaini577 – 1155579Probable translation initiation factor IF-2, 2nd partSequence AnalysisPRO_0000014486Add
BLAST

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.Curated

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Interactioni

Protein-protein interaction databases

STRINGi243232.MJ_0262.

Structurei

3D structure databases

ProteinModelPortaliQ57710.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini237 – 367131DOD-type homing endonucleaseAdd
BLAST
Domaini561 – 781221tr-type GAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0532.
InParanoidiQ57710.
KOiK03243.
OMAiDHGKCLL.
PhylomeDBiQ57710.

Family and domain databases

Gene3Di2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
3.40.50.10050. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00100_A. IF_2_A.
InterProiIPR029459. EFTU-type.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR030934. Intein_C.
IPR004042. Intein_endonuc.
IPR006141. Intein_N.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR004544. TIF_aIF-2_arc.
IPR023115. TIF_IF2_dom3.
IPR009000. Transl_B-barrel.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PfamiPF03144. GTP_EFTU_D2. 1 hit.
PF14578. GTP_EFTU_D4. 1 hit.
PF11987. IF-2. 1 hit.
PF14528. LAGLIDADG_3. 1 hit.
[Graphical view]
PRINTSiPR00379. INTEIN.
SMARTiSM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF51294. SSF51294. 3 hits.
SSF52156. SSF52156. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF55608. SSF55608. 1 hit.
TIGRFAMsiTIGR00491. aIF-2. 1 hit.
TIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS51722. G_TR_2. 1 hit.
PS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q57710-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKKNTKKDN KNQNLRCPIV CVLGHVDHGK CLMPHEKVLT EYGEIKIEDL
60 70 80 90 100
FKIGKEIVEK DELKEIRKLN IKVHTLNENG EIKIINAPYV WKLKHKGKMI
110 120 130 140 150
KVKLKNWHSI TTTPEHPFLT NNGWIKAENI KKGMYVAIPR KIYGNEDFEK
160 170 180 190 200
FIEFINSKIL TNELIVKVNE KDLKNVELPS TKIYKKQKNV FRSEDIIEHN
210 220 230 240 250
LNIEKISFSP RIHRCGKPQH YIKLPKSLNE WKAIFYFAGV MFGDGCVDRI
260 270 280 290 300
ANNDEEVFNK LKSLNNLGIE VERIKRKSSY EIIFKNGKNA LINLLKILFD
310 320 330 340 350
YPSEKKSHNI KIPQILYIAP KELVAEFIKG YFDADGYVNL RQNRIEVISA
360 370 380 390 400
SKEFIEGLSI LLLRFEITSK IYEIKKSYKE TKKKYYQLNI VGKRNLKNFK
410 420 430 440 450
NIGFSIKYKE ENLNKIIEKS RKSEKYPINK DMKRLRILFG MTRNEVNVSY
460 470 480 490 500
YAKYENGKEI PSYEIVKKFL NSLKPKNLDK KIKVLEGKER DVNYLKAFES
510 520 530 540 550
DGLIENGRLT KLGREALNIW KNHEFGKENI DYMKSLIENI AFVEVEDVEI
560 570 580 590 600
IDYDGYVYDL TTETHNFIAN GIVVHNTTLL DKIRKTRVAK REAGGITQHI
610 620 630 640 650
GASEIPIDVI KRLCGDLLKM LKADLKIPGL LVIDTPGHEA FTSLRKRGGA
660 670 680 690 700
LADIAILVVD INEGFKPQTV EAVNILRQCK TPFVVAANKI DLIPGWNSKE
710 720 730 740 750
GPFILNFNEK NQHPNALTEF EIRLYENIIK PLNELGFDAD LYSRVQDVTK
760 770 780 790 800
TVCIIPVSAV TGEGIPDLLM MVAGLAQKFL EDRLKLNVEG YAKGTILEVK
810 820 830 840 850
EEKGLGTTID AIIYDGIAKR GDYLVVGLPD DVLVTRVKAL LKPKPLDEMR
860 870 880 890 900
DPRDKFKPVN EVTAAAGVKI AAPELDKVIA GCPIRIVPKD KIEEAKEEVM
910 920 930 940 950
KEVEEAKIEV DDEGILIKAD TLGSLEALAN ELRKAGVKIK KAEVGDVTKK
960 970 980 990 1000
DVIEVASYKQ SNPLHGAIVA FNVKILPEAQ KEIEKYDIKV FLDNIIYKLV
1010 1020 1030 1040 1050
EDFTEWIKKE EERIKYGEFE KLIKPAIIRI LPDCIFRQKD PAICGVEVLC
1060 1070 1080 1090 1100
GTLRVGAPLM REDGMQLGYV REIKDRGENV KEAKAGKAVS IAIDGRVVLK
1110 1120 1130 1140 1150
RHVDEGDYMY VAVPESHVRE LYHKYMDRLR NDEKEALLRY MELMQKLTNN

IFWGR
Length:1,155
Mass (Da):132,299
Last modified:November 1, 1996 - v1
Checksum:i6653FA355E7EE0D3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB98248.1.
PIRiG64332.
RefSeqiNP_247234.1. NC_000909.1.
WP_010869759.1. NC_000909.1.

Genome annotation databases

EnsemblBacteriaiAAB98248; AAB98248; MJ_0262.
GeneIDi1451116.
KEGGimja:MJ_0262.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB98248.1.
PIRiG64332.
RefSeqiNP_247234.1. NC_000909.1.
WP_010869759.1. NC_000909.1.

3D structure databases

ProteinModelPortaliQ57710.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243232.MJ_0262.

Protein family/group databases

MEROPSiN10.004.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB98248; AAB98248; MJ_0262.
GeneIDi1451116.
KEGGimja:MJ_0262.

Phylogenomic databases

eggNOGiCOG0532.
InParanoidiQ57710.
KOiK03243.
OMAiDHGKCLL.
PhylomeDBiQ57710.

Family and domain databases

Gene3Di2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
3.40.50.10050. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00100_A. IF_2_A.
InterProiIPR029459. EFTU-type.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR030934. Intein_C.
IPR004042. Intein_endonuc.
IPR006141. Intein_N.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR004544. TIF_aIF-2_arc.
IPR023115. TIF_IF2_dom3.
IPR009000. Transl_B-barrel.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PfamiPF03144. GTP_EFTU_D2. 1 hit.
PF14578. GTP_EFTU_D4. 1 hit.
PF11987. IF-2. 1 hit.
PF14528. LAGLIDADG_3. 1 hit.
[Graphical view]
PRINTSiPR00379. INTEIN.
SMARTiSM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF51294. SSF51294. 3 hits.
SSF52156. SSF52156. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF55608. SSF55608. 1 hit.
TIGRFAMsiTIGR00491. aIF-2. 1 hit.
TIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS51722. G_TR_2. 1 hit.
PS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Entry informationi

Entry nameiIF2P_METJA
AccessioniPrimary (citable) accession number: Q57710
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: June 24, 2015
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The intein interrupts the GTP-binding site.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.