Q576P9 (UPP_BRUAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uracil phosphoribosyltransferase
EC=2.4.2.9

Alternative name(s):
UMP pyrophosphorylase
UPRTase

Gene names

Name:	upp
Ordered Locus Names:	BruAb2_1007

Organism

Brucella abortus biovar 1 (strain 9-941) [Complete proteome] [HAMAP]

Taxonomic identifier

262698 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella abortus › Brucella abortus bv. 1

Protein attributes

Sequence length	208 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of uracil and 5-phospho-alpha-D-ribose 1-diphosphate (PRPP) to UMP and diphosphate By similarity. HAMAP-Rule MF_01218
Catalytic activity	UMP + diphosphate = uracil + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP-Rule MF_01218
Cofactor	Binds 1 Mg2+ ion per subunit. The magnesium is bound as Mg-PRPP By similarity.
Enzyme regulation	Allosterically activated by GTP By similarity. HAMAP-Rule MF_01218
Pathway	Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uracil: step 1/1. HAMAP-Rule MF_01218
Sequence similarities	Belongs to the UPRTase family.

Ontologies

Keywords
Ligand	GTP-binding Magnesium Nucleotide-binding
Molecular function	Glycosyltransferase Transferase
Technical term	Allosteric enzyme Complete proteome
Gene Ontology (GO)
Biological_process	UMP salvage Inferred from electronic annotation. Source: UniProtKB-UniPathway uracil salvage Inferred from electronic annotation. Source: InterPro
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: HAMAP uracil phosphoribosyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 208	208	Uracil phosphoribosyltransferase HAMAP-Rule MF_01218		PRO_1000053681
Regions
	Region	130 – 138	9	5-phospho-alpha-D-ribose 1-diphosphate binding By similarity
	Region	198 – 200	3	Uracil binding By similarity
Sites
	Binding site	78	1	5-phospho-alpha-D-ribose 1-diphosphate By similarity
	Binding site	103	1	5-phospho-alpha-D-ribose 1-diphosphate By similarity
	Binding site	193	1	Uracil; via amide nitrogen By similarity
	Binding site	199	1	5-phospho-alpha-D-ribose 1-diphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q576P9 [UniParc]. Last modified May 10, 2005. Version 1. Checksum: 00C34084C66B701B	FASTA	208	22,994
	10 20 30 40 50 60 MGVTVVSHPL VKHKLTIMRK KETSTASFRR LLKEISLLLC YEVTRNLELT TMSIETPLMP 70 80 90 100 110 120 MEAPVLEGKK LVFASILRAG NGLLEGMLDL VPAARVAHIG LYRDHDTLQP IEYYFKAPED 130 140 150 160 170 180 IVNRLVIVVD PMLATANSAI AAIDKLKERG ATNIRFLCLL AAPEGIERFT KAHPDVEVFT 190 200 ASIDECLDEK GYIVPGLGDA GDRMYGTK

References

[1]

"Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis." Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z., Li L.-L., Kapur V., Alt D.P., Olsen S.C. J. Bacteriol. 187:2715-2726(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 9-941.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017224 Genomic DNA. Translation: AAX76385.1.
RefSeq	YP_223746.1. NC_006933.1.
3D structure databases
HSSP	HSSP built from PDB template 1I5E based on UniProtKB P70881.
ProteinModelPortal	Q576P9.
SMR	Q576P9. Positions 2-208.
ModBase	Search...
Protein-protein interaction databases
STRING	262698.BruAb2_1007.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAX76385; AAX76385; BruAb2_1007.
GeneID	3341682.
KEGG	bmb:BruAb2_1007.
PATRIC	17828331. VBIBruAbo15061_3333.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0035.
HOGENOM	HOG000262754.
KO	K00761.
OMA	YEVTRDM.
ProtClustDB	PRK00129.
Enzyme and pathway databases
BioCyc	BABO262698:GJC2-3227-MONOMER.
UniPathway	UPA00574; UER00636.
Family and domain databases
HAMAP	MF_01218_B. Upp_B.
InterPro	IPR000836. PRibTrfase_dom. IPR005765. Ura_phspho_trans. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01091. upp. 1 hit.
ProtoNet	Search...

Entry information

Entry name

UPP_BRUAB

Accession

Primary (citable) accession number: Q576P9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 10, 2005
Last modified:	May 1, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program