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Protein

Chorismate mutase

Gene

aroQ

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of chorismate into prephenate via a Claisen rearrangement.1 Publication

Catalytic activityi

Chorismate = prephenate.1 Publication

Kineticsi

kcat is 5.7 sec(-1) (at 30 degrees Celsius).
  1. KM=41 µM for chorismate (at 30 degrees Celsius)1 Publication

    pH dependencei

    Activity is independent of pH in the range of pH 5-9.1 Publication

    Temperature dependencei

    Thermostable. The midpoint for its thermal unfolding transition (Tm) is 88 degrees Celsius.1 Publication

    Pathwayi: prephenate biosynthesis

    This protein is involved in step 1 of the subpathway that synthesizes prephenate from chorismate.
    Proteins known to be involved in this subpathway in this organism are:
    1. Chorismate mutase (aroQ)
    This subpathway is part of the pathway prephenate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes prephenate from chorismate, the pathway prephenate biosynthesis and in Metabolic intermediate biosynthesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei26SubstrateBy similarity1
    Binding sitei37SubstrateBy similarity1
    Binding sitei46SubstrateBy similarity1
    Binding sitei50SubstrateBy similarity1
    Binding sitei86SubstrateBy similarity1

    GO - Molecular functioni

    • chorismate mutase activity Source: UniProtKB-EC
    • prephenate dehydratase activity Source: GO_Central
    • protein homodimerization activity Source: CAFA

    GO - Biological processi

    Keywordsi

    Molecular functionIsomerase

    Enzyme and pathway databases

    SABIO-RKiQ57696
    UniPathwayiUPA00120; UER00203

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Chorismate mutase (EC:5.4.99.5)
    Short name:
    CM
    Alternative name(s):
    Monofunctional chorismate mutase AroQ(f)
    Gene namesi
    Name:aroQ
    Synonyms:aroQ(f)
    Ordered Locus Names:MJ0246
    OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
    Taxonomic identifieri243232 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
    Proteomesi
    • UP000000805 Componenti: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001192021 – 99Chorismate mutaseAdd BLAST99

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    GO - Molecular functioni

    • protein homodimerization activity Source: CAFA

    Protein-protein interaction databases

    STRINGi243232.MJ_0246

    Structurei

    Secondary structure

    199
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Helixi4 – 20Combined sources17
    Helixi23 – 39Combined sources17
    Helixi47 – 64Combined sources18
    Helixi69 – 93Combined sources25

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2GTVNMR-X1-93[»]
    DisProtiDP00465
    ProteinModelPortaliQ57696
    SMRiQ57696
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ57696

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini1 – 90Chorismate mutasePROSITE-ProRule annotationAdd BLAST90

    Phylogenomic databases

    eggNOGiarCOG02098 Archaea
    COG1605 LUCA
    InParanoidiQ57696
    KOiK04093
    OMAiIARRTYV
    OrthoDBiPOG093Z0J25
    PhylomeDBiQ57696

    Family and domain databases

    Gene3Di1.20.59.10, 1 hit
    InterProiView protein in InterPro
    IPR036263 Chorismate_II_sf
    IPR010950 Chorismate_mutase_arc
    IPR036979 CM_dom_sf
    IPR002701 CM_II_prokaryot
    PfamiView protein in Pfam
    PF01817 CM_2, 1 hit
    SMARTiView protein in SMART
    SM00830 CM_2, 1 hit
    SUPFAMiSSF48600 SSF48600, 1 hit
    TIGRFAMsiTIGR01791 CM_archaeal, 1 hit
    PROSITEiView protein in PROSITE
    PS51168 CHORISMATE_MUT_2, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q57696-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MIEKLAEIRK KIDEIDNKIL KLIAERNSLA KDVAEIKNQL GIPINDPERE
    60 70 80 90
    KYIYDRIRKL CKEHNVDENI GIKIFQILIE HNKALQKQYL EETQNKNKK
    Length:99
    Mass (Da):11,782
    Last modified:November 1, 1996 - v1
    Checksum:i9759018D0A07E76C
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    L77117 Genomic DNA Translation: AAB98234.1
    PIRiG64330

    Genome annotation databases

    EnsemblBacteriaiAAB98234; AAB98234; MJ_0246
    KEGGimja:MJ_0246

    Entry informationi

    Entry nameiCHMU_METJA
    AccessioniPrimary (citable) accession number: Q57696
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: March 28, 2018
    This is version 103 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Methanococcus jannaschii
      Methanococcus jannaschii: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
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