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Q57688

- THIDN_METJA

UniProt

Q57688 - THIDN_METJA

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Protein
Bifunctional thiamine biosynthesis protein ThiDN
Gene
thiDN, MJ0236
Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P By similarity.
Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) By similarity.

Catalytic activityi

ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine = ADP + 4-amino-5-phosphonooxymethyl-2-methylpyrimidine.
ATP + 4-amino-2-methyl-5-phosphomethylpyrimidine = ADP + 4-amino-2-methyl-5-diphosphomethylpyrimidine.
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole = diphosphate + thiamine phosphate.

Pathwayi

Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-amino-2-methyl-5-diphosphomethylpyrimidine from 5-amino-1-(5-phospho-D-ribosyl)imidazole.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei41 – 411Hydroxymethylpyrimidine/phosphomethylpyrimidine By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. hydroxymethylpyrimidine kinase activity Source: UniProtKB-EC
  3. phosphomethylpyrimidine kinase activity Source: UniProtKB-EC
  4. thiamine-phosphate diphosphorylase activity Source: UniProtKB-EC

GO - Biological processi

  1. thiamine biosynthetic process Source: UniProtKB-KW
  2. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00060; UER00141.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional thiamine biosynthesis protein ThiDN
Including the following 2 domains:
Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase (EC:2.7.1.49, EC:2.7.4.7)
Alternative name(s):
Hydroxymethylpyrimidine kinase
Short name:
HMP kinase
Hydroxymethylpyrimidine phosphate kinase
Short name:
HMP-P kinase
Short name:
HMP-phosphate kinase
Short name:
HMPP kinase
Thiamine-phosphate synthase ThiN (EC:2.5.1.3)
Short name:
TP synthase
Short name:
TPS
Alternative name(s):
Thiamine-phosphate pyrophosphorylase
Short name:
TMP pyrophosphorylase
Short name:
TMP-PPase
Gene namesi
Name:thiDN
Ordered Locus Names:MJ0236
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
ProteomesiUP000000805: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 417417Bifunctional thiamine biosynthesis protein ThiDN
PRO_0000106755Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi243232.MJ0236.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi240 – 25516
Beta strandi269 – 2724
Helixi279 – 2813
Beta strandi282 – 2876
Beta strandi289 – 2913
Beta strandi295 – 2995
Beta strandi303 – 3053
Helixi309 – 31911
Beta strandi327 – 3315
Helixi335 – 3417
Turni342 – 3443
Beta strandi347 – 3493
Helixi352 – 3543
Helixi361 – 37313
Beta strandi378 – 3825
Beta strandi391 – 3988
Helixi399 – 41416

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2PHPX-ray2.03A/B/D/E236-416[»]
ProteinModelPortaliQ57688.

Miscellaneous databases

EvolutionaryTraceiQ57688.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 235235Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase
Add
BLAST
Regioni236 – 417182Thiamine-phosphate synthase
Add
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the ThiD family.
In the C-terminal section; belongs to the ThiN family.

Phylogenomic databases

eggNOGiCOG1992.
KOiK00941.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
3.40.225.10. 1 hit.
InterProiIPR001303. Aldolase_II/adducin_N.
IPR013749. PM/HMP-P_kinase-1.
IPR029056. Ribokinase-like.
IPR019293. ThiN.
[Graphical view]
PfamiPF10120. Aldolase_2. 1 hit.
PF08543. Phos_pyr_kin. 1 hit.
[Graphical view]
SUPFAMiSSF53613. SSF53613. 1 hit.
SSF53639. SSF53639. 1 hit.

Sequencei

Sequence statusi: Complete.

Q57688-1 [UniParc]FASTAAdd to Basket

« Hide

MVILAIGGYD PTSGAGISAD IKTAHTLGVY CPTITTSVIP QNNKMVYEKF    50
DLPEENIKNQ FKAVFEEFDI EYVKTGVLTK PAIDTLLKYI DKYDLKVICD 100
PVLASTTKFS FVDEKLMEKY IELFNKSFLI TPNKEEYKKI MEFIKNNNLM 150
IRNDLYILAT GIDDILMKNF KPIKTFKGFR VDKEVHGTGC VYSTAITAFL 200
SKGYDLEEAI KEAKRFVLSS VIYAKKSKFG YNSNPTYINK EKVIKNLSYA 250
IYLLKKMNFT LIPEVGSNIA ESLPFPKDFK DVAALTGRII KNKLGGFYIV 300
GDIEFGASEH IAKIILSASK FNPEIRACMN IKYDGGLIKL LKDKFAVSSF 350
DRKEEPPNVS TMEWGTKIAC EKFGGVPDII YDRGGEGKEP MIRVLGRDAI 400
EVVKKVEVIQ KIYNTLM 417
Length:417
Mass (Da):47,056
Last modified:January 25, 2012 - v2
Checksum:i810246AD46805E27
GO

Sequence cautioni

The sequence AAB98222.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L77117 Genomic DNA. Translation: AAB98222.1. Different initiation.
PIRiE64329.
RefSeqiNP_247207.1. NC_000909.1.

Genome annotation databases

EnsemblBacteriaiAAB98222; AAB98222; MJ_0236.
GeneIDi1451089.
KEGGimja:MJ_0236.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L77117 Genomic DNA. Translation: AAB98222.1 . Different initiation.
PIRi E64329.
RefSeqi NP_247207.1. NC_000909.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2PHP X-ray 2.03 A/B/D/E 236-416 [» ]
ProteinModelPortali Q57688.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 243232.MJ0236.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAB98222 ; AAB98222 ; MJ_0236 .
GeneIDi 1451089.
KEGGi mja:MJ_0236.

Phylogenomic databases

eggNOGi COG1992.
KOi K00941.

Enzyme and pathway databases

UniPathwayi UPA00060 ; UER00141 .

Miscellaneous databases

EvolutionaryTracei Q57688.

Family and domain databases

Gene3Di 3.40.1190.20. 1 hit.
3.40.225.10. 1 hit.
InterProi IPR001303. Aldolase_II/adducin_N.
IPR013749. PM/HMP-P_kinase-1.
IPR029056. Ribokinase-like.
IPR019293. ThiN.
[Graphical view ]
Pfami PF10120. Aldolase_2. 1 hit.
PF08543. Phos_pyr_kin. 1 hit.
[Graphical view ]
SUPFAMi SSF53613. SSF53613. 1 hit.
SSF53639. SSF53639. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.
  2. "Crystal structure of the C-terminal domain of protein MJ0236 (Y236_METJA)."
    New York structural genomix research consortium (NYSGXRC)
    Submitted (FEB-2009) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.03 ANGSTROMS) OF 236-416.

Entry informationi

Entry nameiTHIDN_METJA
AccessioniPrimary (citable) accession number: Q57688
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 25, 2012
Last modified: September 3, 2014
This is version 76 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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