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Protein

Non-canonical purine NTP pyrophosphatase

Gene

MJ0226

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP to their respective monophosphate derivatives. Probably excludes non-canonical purines from DNA precursor pool, thus preventing their incorporation into DNA and avoiding chromosomal lesions.1 Publication

Catalytic activityi

A nucleoside triphosphate + H2O = a nucleotide + diphosphate.1 Publication

Cofactori

Mg2+1 Publication, Mn2+1 PublicationNote: Binds 1 divalent cation per subunit; can use either Mg2+ or Mn2+.1 Publication

Kineticsi

  1. KM=0.10 mM for XTP1 Publication
  2. KM=0.15 mM for ITP1 Publication
  3. KM=1.11 mM for GTP1 Publication
  4. KM=1.13 mM for dGTP1 Publication

    Temperature dependencei

    Optimum temperature is 80 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi37Manganese or magnesiumBy similarity1
    Metal bindingi65Manganese or magnesiumBy similarity1
    Binding sitei144SubstrateBy similarity1
    Binding sitei164SubstrateBy similarity1
    Binding sitei170SubstrateBy similarity1

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Nucleotide metabolism

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi3.6.1.66. 3260.
    3.6.1.B14. 3260.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Non-canonical purine NTP pyrophosphatase (EC:3.6.1.9UniRule annotation1 Publication)
    Alternative name(s):
    Non-standard purine NTP pyrophosphatase
    Nucleoside-triphosphate diphosphatase
    Nucleoside-triphosphate pyrophosphatase
    Short name:
    NTPase
    Nucleotide diphosphataseUniRule annotation
    Gene namesi
    Ordered Locus Names:MJ0226
    OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
    Taxonomic identifieri243232 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
    Proteomesi
    • UP000000805 Componenti: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001782741 – 185Non-canonical purine NTP pyrophosphataseAdd BLAST185

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    STRINGi243232.MJ_0226.

    Structurei

    Secondary structure

    1185
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi3 – 6Combined sources4
    Helixi10 – 19Combined sources10
    Turni20 – 22Combined sources3
    Beta strandi28 – 31Combined sources4
    Beta strandi38 – 40Combined sources3
    Helixi42 – 57Combined sources16
    Beta strandi61 – 70Combined sources10
    Helixi71 – 73Combined sources3
    Helixi81 – 87Combined sources7
    Helixi89 – 97Combined sources9
    Beta strandi104 – 115Combined sources12
    Beta strandi118 – 130Combined sources13
    Helixi142 – 145Combined sources4
    Beta strandi146 – 149Combined sources4
    Helixi156 – 158Combined sources3
    Helixi161 – 164Combined sources4
    Turni165 – 167Combined sources3
    Helixi169 – 182Combined sources14

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1B78X-ray2.20A/B1-185[»]
    2MJPX-ray2.20A/B1-185[»]
    ProteinModelPortaliQ57679.
    SMRiQ57679.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ57679.

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni7 – 12Substrate bindingBy similarity6
    Regioni65 – 66Substrate bindingBy similarity2

    Sequence similaritiesi

    Belongs to the HAM1 NTPase family.Curated

    Phylogenomic databases

    eggNOGiarCOG04184. Archaea.
    COG0127. LUCA.
    InParanoidiQ57679.
    KOiK02428.

    Family and domain databases

    CDDicd00515. HAM1. 1 hit.
    Gene3Di3.90.950.10. 1 hit.
    HAMAPiMF_01405. Non_canon_purine_NTPase. 1 hit.
    InterProiIPR002637. Ham1p-like.
    IPR029001. ITPase-like_fam.
    IPR020922. NTPase.
    [Graphical view]
    PANTHERiPTHR11067. PTHR11067. 1 hit.
    PfamiPF01725. Ham1p_like. 1 hit.
    [Graphical view]
    SUPFAMiSSF52972. SSF52972. 1 hit.
    TIGRFAMsiTIGR00042. TIGR00042. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q57679-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKIYFATGNP NKIKEANIIL KDLKDVEIEQ IKISYPEIQG TLEEVAEFGA
    60 70 80 90 100
    KWVYNILKKP VIVEDSGFFV EALNGFPGTY SKFVQETIGN EGILKLLEGK
    110 120 130 140 150
    DNRNAYFKTV IGYCDENGVR LFKGIVKGRV SEEIRSKGYG FAYDSIFIPE
    160 170 180
    EEERTFAEMT TEEKSQISHR KKAFEEFKKF LLDRI
    Length:185
    Mass (Da):21,273
    Last modified:June 28, 2011 - v2
    Checksum:i97EC2DA5C0D21D67
    GO

    Sequence cautioni

    The sequence AAB98211 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    L77117 Genomic DNA. Translation: AAB98211.1. Different initiation.
    PIRiC64328.
    RefSeqiWP_064496428.1. NC_000909.1.

    Genome annotation databases

    EnsemblBacteriaiAAB98211; AAB98211; MJ_0226.
    GeneIDi1451077.
    KEGGimja:MJ_0226.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    L77117 Genomic DNA. Translation: AAB98211.1. Different initiation.
    PIRiC64328.
    RefSeqiWP_064496428.1. NC_000909.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1B78X-ray2.20A/B1-185[»]
    2MJPX-ray2.20A/B1-185[»]
    ProteinModelPortaliQ57679.
    SMRiQ57679.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi243232.MJ_0226.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAB98211; AAB98211; MJ_0226.
    GeneIDi1451077.
    KEGGimja:MJ_0226.

    Phylogenomic databases

    eggNOGiarCOG04184. Archaea.
    COG0127. LUCA.
    InParanoidiQ57679.
    KOiK02428.

    Enzyme and pathway databases

    BRENDAi3.6.1.66. 3260.
    3.6.1.B14. 3260.

    Miscellaneous databases

    EvolutionaryTraceiQ57679.

    Family and domain databases

    CDDicd00515. HAM1. 1 hit.
    Gene3Di3.90.950.10. 1 hit.
    HAMAPiMF_01405. Non_canon_purine_NTPase. 1 hit.
    InterProiIPR002637. Ham1p-like.
    IPR029001. ITPase-like_fam.
    IPR020922. NTPase.
    [Graphical view]
    PANTHERiPTHR11067. PTHR11067. 1 hit.
    PfamiPF01725. Ham1p_like. 1 hit.
    [Graphical view]
    SUPFAMiSSF52972. SSF52972. 1 hit.
    TIGRFAMsiTIGR00042. TIGR00042. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiNTPA_METJA
    AccessioniPrimary (citable) accession number: Q57679
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: June 28, 2011
    Last modified: November 30, 2016
    This is version 114 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    XTP is the best substrate, followed by ITP, GTP or dGTP, both of which are hydrolyzed 100-fold less efficiently than XTP, and finally ATP, CTP and TTP which are hydrolyzed the least well. XDP or GDP are not hydrolyzed to XMP or GMP, suggesting that Mj0226 is a pyrophosphate-releasing NTPase.

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Methanococcus jannaschii
      Methanococcus jannaschii: entries and gene names
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.