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Q57657

- PUR1_METJA

UniProt

Q57657 - PUR1_METJA

Protein

Probable amidophosphoribosyltransferase

Gene

purF

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.

    Cofactori

    Binds 1 magnesium ion per subunit.By similarity
    Binds 1 4Fe-4S cluster per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei2 – 21For GATase activityBy similarity
    Metal bindingi255 – 2551Iron-sulfur (4Fe-4S)By similarity
    Metal bindingi302 – 3021MagnesiumBy similarity
    Metal bindingi364 – 3641MagnesiumBy similarity
    Metal bindingi365 – 3651MagnesiumBy similarity
    Metal bindingi401 – 4011Iron-sulfur (4Fe-4S)By similarity
    Metal bindingi450 – 4501Iron-sulfur (4Fe-4S)By similarity
    Metal bindingi453 – 4531Iron-sulfur (4Fe-4S)By similarity

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. amidophosphoribosyltransferase activity Source: UniProtKB-EC
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
    2. glutamine metabolic process Source: UniProtKB-KW
    3. nucleoside metabolic process Source: InterPro
    4. purine nucleobase biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Purine biosynthesis

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Magnesium, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00074; UER00124.

    Protein family/group databases

    MEROPSiC44.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable amidophosphoribosyltransferase (EC:2.4.2.14)
    Short name:
    ATase
    Alternative name(s):
    Glutamine phosphoribosylpyrophosphate amidotransferase
    Short name:
    GPATase
    Gene namesi
    Name:purF
    Ordered Locus Names:MJ0204
    OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
    Taxonomic identifieri243232 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
    ProteomesiUP000000805: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 471470Probable amidophosphoribosyltransferasePRO_0000139644Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi243232.MJ0204.

    Structurei

    3D structure databases

    ProteinModelPortaliQ57657.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 224223Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.Curated
    Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Glutamine amidotransferase

    Phylogenomic databases

    eggNOGiCOG0034.
    KOiK00764.
    OMAiSGIPYEM.
    PhylomeDBiQ57657.

    Family and domain databases

    Gene3Di3.40.50.2020. 1 hit.
    3.60.20.10. 1 hit.
    InterProiIPR005854. Amd_phspho_trans.
    IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view]
    PfamiPF00310. GATase_2. 1 hit.
    PF13537. GATase_7. 1 hit.
    PF00156. Pribosyltran. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
    SUPFAMiSSF53271. SSF53271. 1 hit.
    SSF56235. SSF56235. 1 hit.
    TIGRFAMsiTIGR01134. purF. 1 hit.
    PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
    PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q57657-1 [UniParc]FASTAAdd to Basket

    « Hide

    MCGIFGIYSY ERLNVAKKIY YGLFALQHRG QEGAGIATSD GKNIHYYKNI    50
    GLVTDVFKNE TLQNLFGYIG IGHVRYSTTG GKAVENCQPF VVKSSFGNIA 100
    IAHNGDLVNS DELRRELEMK GHIFTSSTDS EVIAQLLVRE LLKTSDKIEA 150
    IKNTLKKLVG AYSLLIMFND SLIAVRDPWG FKPLCIGRDE SNIYISSEDC 200
    ALTTLDAEFV KDIEPGEIIE IKDGEIISHK LDYGVSEYNP VNVDVPCIYR 250
    GAATCMFEYV YFARPDSTID GISVYKVRKR IGKILAKEHP VDADVVSPIP 300
    DSGVTFALGF SEESGIPYYE GLIKNRYVGR TFILPSQNER ELAVRLKLSP 350
    VKSVLEGKRV VLVDDSIVRG TTSRRIVNMV RKAGAKEVHL RIGCPKIISP 400
    CYYGIDMATK KELIASNKTE EEIGKAIGVD SIGYLSLEGL VKAIGRKDLC 450
    LACVTGKYPT EVNFEKILGR E 471
    Length:471
    Mass (Da):52,187
    Last modified:January 23, 2007 - v3
    Checksum:i27E25B66A8D5120A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L77117 Genomic DNA. Translation: AAB98188.1.
    PIRiE64325.
    RefSeqiNP_247172.1. NC_000909.1.
    WP_010869699.1. NC_000909.1.

    Genome annotation databases

    EnsemblBacteriaiAAB98188; AAB98188; MJ_0204.
    GeneIDi1451053.
    KEGGimja:MJ_0204.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L77117 Genomic DNA. Translation: AAB98188.1 .
    PIRi E64325.
    RefSeqi NP_247172.1. NC_000909.1.
    WP_010869699.1. NC_000909.1.

    3D structure databases

    ProteinModelPortali Q57657.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243232.MJ0204.

    Protein family/group databases

    MEROPSi C44.001.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAB98188 ; AAB98188 ; MJ_0204 .
    GeneIDi 1451053.
    KEGGi mja:MJ_0204.

    Phylogenomic databases

    eggNOGi COG0034.
    KOi K00764.
    OMAi SGIPYEM.
    PhylomeDBi Q57657.

    Enzyme and pathway databases

    UniPathwayi UPA00074 ; UER00124 .

    Family and domain databases

    Gene3Di 3.40.50.2020. 1 hit.
    3.60.20.10. 1 hit.
    InterProi IPR005854. Amd_phspho_trans.
    IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view ]
    Pfami PF00310. GATase_2. 1 hit.
    PF13537. GATase_7. 1 hit.
    PF00156. Pribosyltran. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000485. Amd_phspho_trans. 1 hit.
    SUPFAMi SSF53271. SSF53271. 1 hit.
    SSF56235. SSF56235. 1 hit.
    TIGRFAMsi TIGR01134. purF. 1 hit.
    PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
    PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

    Entry informationi

    Entry nameiPUR1_METJA
    AccessioniPrimary (citable) accession number: Q57657
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 102 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Methanococcus jannaschii
      Methanococcus jannaschii: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3