Reviewed, UniProtKB/Swiss-Prot Q57657 (PUR1_METJA)

Names and origin

Protein names

Recommended name:
    Probable amidophosphoribosyltransferase
      Short name=ATase
    EC=2.4.2.14
Alternative name(s):
    Glutamine phosphoribosylpyrophosphate amidotransferase
      Short name=GPATase

Gene names

Name:	purF
Ordered Locus Names:	MJ0204

Organism

Methanocaldococcus jannaschii (Methanococcus jannaschii) [Complete proteome] [HAMAP]

Taxonomic identifier

2190 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	471 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.
Cofactor	Binds 1 magnesium ion per subunit By similarity. Binds 1 4Fe-4S cluster per subunit By similarity.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.
Sequence similarities	In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family. Contains 1 glutamine amidotransferase type-2 domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Domain	Glutamine amidotransferase
Ligand	4Fe-4S Iron Iron-sulfur Magnesium Metal-binding
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW nucleoside metabolic process Inferred from electronic annotation. Source: InterPro purine base biosynthetic process Inferred from electronic annotation. Source: InterPro purine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW amidophosphoribosyltransferase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 471	470	Probable amidophosphoribosyltransferase		PRO_0000139644
Regions
	Domain	2 – 224	223	Glutamine amidotransferase type-2
Sites
	Active site	2	1	For GATase activity By similarity
	Metal binding	255	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	302	1	Magnesium By similarity
	Metal binding	364	1	Magnesium By similarity
	Metal binding	365	1	Magnesium By similarity
	Metal binding	401	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	450	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	453	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q57657-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 27E25B66A8D5120A	FASTA	471	52,187
	10 20 30 40 50 60 MCGIFGIYSY ERLNVAKKIY YGLFALQHRG QEGAGIATSD GKNIHYYKNI GLVTDVFKNE 70 80 90 100 110 120 TLQNLFGYIG IGHVRYSTTG GKAVENCQPF VVKSSFGNIA IAHNGDLVNS DELRRELEMK 130 140 150 160 170 180 GHIFTSSTDS EVIAQLLVRE LLKTSDKIEA IKNTLKKLVG AYSLLIMFND SLIAVRDPWG 190 200 210 220 230 240 FKPLCIGRDE SNIYISSEDC ALTTLDAEFV KDIEPGEIIE IKDGEIISHK LDYGVSEYNP 250 260 270 280 290 300 VNVDVPCIYR GAATCMFEYV YFARPDSTID GISVYKVRKR IGKILAKEHP VDADVVSPIP 310 320 330 340 350 360 DSGVTFALGF SEESGIPYYE GLIKNRYVGR TFILPSQNER ELAVRLKLSP VKSVLEGKRV 370 380 390 400 410 420 VLVDDSIVRG TTSRRIVNMV RKAGAKEVHL RIGCPKIISP CYYGIDMATK KELIASNKTE 430 440 450 460 470 EEIGKAIGVD SIGYLSLEGL VKAIGRKDLC LACVTGKYPT EVNFEKILGR E

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L77117 Genomic DNA. Translation: AAB98188.1.
PIR	E64325.
RefSeq	NP_247172.1.
3D structure databases
SMR	Q57657. Positions 2-468.
ModBase	Search...
Genome annotation databases
GeneID	1451053.
GenomeReviews	Gene locus MJ0204 in contig L77117_GR.
KEGG	mja:MJ0204.
NMPDR	fig|243232.1.peg.209.
TIGR	MJ0204.
Phylogenomic databases
HOGENOM	HBG392416.
OMA	SSETEGW.
Enzyme and pathway databases
BRENDA	2.4.2.14. 256362.
Family and domain databases
InterPro	IPR005854. Amd_phspho_trans. IPR000583. GATase_2. IPR017932. GATase_II. IPR000836. PRibTrfase. [Graphical view]
PANTHER	PTHR11907. Amd_phspho_trans. 1 hit.
Pfam	PF00310. GATase_2. 2 hits. PF00156. Pribosyltran. 1 hit. [Graphical view]
PIRSF	PIRSF000485. Amd_phspho_trans. 1 hit.
TIGRFAMs	TIGR01134. purF. 1 hit.
PROSITE	PS51278. GATASE_TYPE_2. 1 hit. PS00103. PUR_PYR_PR_TRANSFER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR1_METJA

Accession

Primary (citable) accession number: Q57657

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	January 23, 2007
Last modified:	February 9, 2010
This is version 74 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)