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Q57657 (PUR1_METJA) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable amidophosphoribosyltransferase

Short name=ATase
EC=2.4.2.14
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase
Short name=GPATase
Gene names
Name:purF
Ordered Locus Names:MJ0204
OrganismMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) [Reference proteome] [HAMAP]
Taxonomic identifier243232 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus

Protein attributes

Sequence length471 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.

Cofactor

Binds 1 magnesium ion per subunit By similarity.

Binds 1 4Fe-4S cluster per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.

Sequence similarities

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.

Contains 1 glutamine amidotransferase type-2 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 471470Probable amidophosphoribosyltransferase
PRO_0000139644

Regions

Domain2 – 224223Glutamine amidotransferase type-2

Sites

Active site21For GATase activity By similarity
Metal binding2551Iron-sulfur (4Fe-4S) By similarity
Metal binding3021Magnesium By similarity
Metal binding3641Magnesium By similarity
Metal binding3651Magnesium By similarity
Metal binding4011Iron-sulfur (4Fe-4S) By similarity
Metal binding4501Iron-sulfur (4Fe-4S) By similarity
Metal binding4531Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q57657 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 27E25B66A8D5120A

FASTA47152,187
        10         20         30         40         50         60 
MCGIFGIYSY ERLNVAKKIY YGLFALQHRG QEGAGIATSD GKNIHYYKNI GLVTDVFKNE 

        70         80         90        100        110        120 
TLQNLFGYIG IGHVRYSTTG GKAVENCQPF VVKSSFGNIA IAHNGDLVNS DELRRELEMK 

       130        140        150        160        170        180 
GHIFTSSTDS EVIAQLLVRE LLKTSDKIEA IKNTLKKLVG AYSLLIMFND SLIAVRDPWG 

       190        200        210        220        230        240 
FKPLCIGRDE SNIYISSEDC ALTTLDAEFV KDIEPGEIIE IKDGEIISHK LDYGVSEYNP 

       250        260        270        280        290        300 
VNVDVPCIYR GAATCMFEYV YFARPDSTID GISVYKVRKR IGKILAKEHP VDADVVSPIP 

       310        320        330        340        350        360 
DSGVTFALGF SEESGIPYYE GLIKNRYVGR TFILPSQNER ELAVRLKLSP VKSVLEGKRV 

       370        380        390        400        410        420 
VLVDDSIVRG TTSRRIVNMV RKAGAKEVHL RIGCPKIISP CYYGIDMATK KELIASNKTE 

       430        440        450        460        470 
EEIGKAIGVD SIGYLSLEGL VKAIGRKDLC LACVTGKYPT EVNFEKILGR E 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L77117 Genomic DNA. Translation: AAB98188.1.
PIRE64325.
RefSeqNP_247172.1. NC_000909.1.

3D structure databases

ProteinModelPortalQ57657.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243232.MJ0204.

Protein family/group databases

MEROPSC44.001.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB98188; AAB98188; MJ_0204.
GeneID1451053.
KEGGmja:MJ_0204.

Phylogenomic databases

eggNOGCOG0034.
KOK00764.
OMASGIPYEM.
PhylomeDBQ57657.

Enzyme and pathway databases

UniPathwayUPA00074; UER00124.

Family and domain databases

Gene3D3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
InterProIPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamPF00310. GATase_2. 1 hit.
PF13537. GATase_7. 1 hit.
PF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsTIGR01134. purF. 1 hit.
PROSITEPS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR1_METJA
AccessionPrimary (citable) accession number: Q57657
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 100 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Methanococcus jannaschii

Methanococcus jannaschii: entries and gene names