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Protein

Amidophosphoribosyltransferase

Gene

purF

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine.UniRule annotation

Catalytic activityi

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotation

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Amidophosphoribosyltransferase (purF)
  2. Phosphoribosylamine--glycine ligase (purD)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2NucleophileUniRule annotation1
Metal bindingi255Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi302MagnesiumUniRule annotation1
Metal bindingi364MagnesiumUniRule annotation1
Metal bindingi365MagnesiumUniRule annotation1
Metal bindingi401Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi450Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi453Iron-sulfur (4Fe-4S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processPurine biosynthesis
Ligand4Fe-4S, Iron, Iron-sulfur, Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciMJAN243232:G1GKE-216-MONOMER
UniPathwayiUPA00074; UER00124

Protein family/group databases

MEROPSiC44.001

Names & Taxonomyi

Protein namesi
Recommended name:
AmidophosphoribosyltransferaseUniRule annotation (EC:2.4.2.14UniRule annotation)
Short name:
ATaseUniRule annotation
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferaseUniRule annotation
Short name:
GPATaseUniRule annotation
Gene namesi
Name:purFUniRule annotation
Ordered Locus Names:MJ0204
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
Proteomesi
  • UP000000805 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001396442 – 471AmidophosphoribosyltransferaseAdd BLAST470

Interactioni

Protein-protein interaction databases

STRINGi243232.MJ_0204

Structurei

3D structure databases

ProteinModelPortaliQ57657
SMRiQ57657
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 224Glutamine amidotransferase type-2UniRule annotationAdd BLAST223

Sequence similaritiesi

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.UniRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiarCOG00093 Archaea
COG0034 LUCA
InParanoidiQ57657
KOiK00764
OMAiFRPLCLG
OrthoDBiPOG093Z01WR
PhylomeDBiQ57657

Family and domain databases

CDDicd00715 GPATase_N, 1 hit
cd06223 PRTases_typeI, 1 hit
Gene3Di3.60.20.10, 3 hits
HAMAPiMF_01931 PurF, 1 hit
InterProiView protein in InterPro
IPR017932 GATase_2_dom
IPR029055 Ntn_hydrolases_N
IPR000836 PRibTrfase_dom
IPR029057 PRTase-like
IPR005854 PurF
IPR035584 PurF_N
PfamiView protein in Pfam
PF13537 GATase_7, 1 hit
PF00156 Pribosyltran, 1 hit
PIRSFiPIRSF000485 Amd_phspho_trans, 1 hit
SUPFAMiSSF53271 SSF53271, 1 hit
SSF56235 SSF56235, 1 hit
TIGRFAMsiTIGR01134 purF, 1 hit
PROSITEiView protein in PROSITE
PS51278 GATASE_TYPE_2, 1 hit
PS00103 PUR_PYR_PR_TRANSFER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q57657-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCGIFGIYSY ERLNVAKKIY YGLFALQHRG QEGAGIATSD GKNIHYYKNI
60 70 80 90 100
GLVTDVFKNE TLQNLFGYIG IGHVRYSTTG GKAVENCQPF VVKSSFGNIA
110 120 130 140 150
IAHNGDLVNS DELRRELEMK GHIFTSSTDS EVIAQLLVRE LLKTSDKIEA
160 170 180 190 200
IKNTLKKLVG AYSLLIMFND SLIAVRDPWG FKPLCIGRDE SNIYISSEDC
210 220 230 240 250
ALTTLDAEFV KDIEPGEIIE IKDGEIISHK LDYGVSEYNP VNVDVPCIYR
260 270 280 290 300
GAATCMFEYV YFARPDSTID GISVYKVRKR IGKILAKEHP VDADVVSPIP
310 320 330 340 350
DSGVTFALGF SEESGIPYYE GLIKNRYVGR TFILPSQNER ELAVRLKLSP
360 370 380 390 400
VKSVLEGKRV VLVDDSIVRG TTSRRIVNMV RKAGAKEVHL RIGCPKIISP
410 420 430 440 450
CYYGIDMATK KELIASNKTE EEIGKAIGVD SIGYLSLEGL VKAIGRKDLC
460 470
LACVTGKYPT EVNFEKILGR E
Length:471
Mass (Da):52,187
Last modified:January 23, 2007 - v3
Checksum:i27E25B66A8D5120A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA Translation: AAB98188.1
PIRiE64325
RefSeqiWP_010869699.1, NC_000909.1

Genome annotation databases

EnsemblBacteriaiAAB98188; AAB98188; MJ_0204
GeneIDi1451053
KEGGimja:MJ_0204

Similar proteinsi

Entry informationi

Entry nameiPUR1_METJA
AccessioniPrimary (citable) accession number: Q57657
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 122 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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