Q57649 (RPON_METJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase subunit N
EC=2.7.7.6

Gene names

Name:	rpoN
Ordered Locus Names:	MJ0196

Organism

Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)

Taxonomic identifier

243232 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	73 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. HAMAP MF_00250
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP MF_00250
Sequence similarities	Belongs to the archaeal rpoN/eukaryotic RPB10 RNA polymerase subunit family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Ligand	Metal-binding Zinc
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 73	73	DNA-directed RNA polymerase subunit N HAMAP MF_00250		PRO_0000121349
Sites
	Metal binding	7	1	Zinc By similarity
	Metal binding	10	1	Zinc By similarity
	Metal binding	44	1	Zinc By similarity
	Metal binding	45	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q57649 [UniParc]. Last modified December 15, 1998. Version 2. Checksum: E716EA406D65B831	FASTA	73	8,695
	10 20 30 40 50 60 MMFPIRCFSC GNVIAEVFEE YKERILKGEN PKDVLDDLGI KKYCCRRMFI SYRIGEDGRE 70 IIDEIIAHDE RYL

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L77117 Genomic DNA. Translation: AAB98176.1.
RefSeq	NP_247164.1. NC_000909.1.
3D structure databases
ProteinModelPortal	Q57649.
SMR	Q57649. Positions 2-52.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1451044.
GenomeReviews	Gene locus MJ0196 in contig L77117_GR.
KEGG	mja:MJ_0196.
NMPDR	fig|243232.1.peg.201.
TIGR	MJ0196.
Phylogenomic databases
HOGENOM	HBG391971.
OMA	KYCCRRM.
ProtClustDB	PRK04016.
Enzyme and pathway databases
BioCyc	MJAN243232:MJ_0196-MONOMER.
Family and domain databases
HAMAP	MF_00250. RNApol_arch_N. [Tree]
InterPro	IPR012287. Homeodomain-rel. IPR000268. RNA_pol_su_N/RPB10. IPR023580. RNA_pol_su_RPB10. IPR020789. RNA_pol_suN_Zn-BS. [Graphical view]
Gene3D	G3DSA:1.10.10.60. Homeodomain-rel. 1 hit.
KO	K03058.
PANTHER	PTHR23413:SF2. RNA_pol_N/8_sub. 1 hit.
Pfam	PF01194. RNA_pol_N. 1 hit. [Graphical view]
PIRSF	PIRSF005653. RNA_pol_N/8_sub. 1 hit.
ProDom	PD006539. RNA_pol_N/8_sub. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF46924. RNA_pol_N/8_sub. 1 hit.
PROSITE	PS01112. RNA_POL_N_8KD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPON_METJA

Accession

Primary (citable) accession number: Q57649

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	December 15, 1998
Last modified:	November 16, 2011
This is version 70 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program