Reviewed, UniProtKB/Swiss-Prot Q57635 (DNLI_METJA)

Names and origin

Protein names

Recommended name:
    DNA ligase
    EC=6.5.1.1
Alternative name(s):
    Polydeoxyribonucleotide synthase [ATP]

Gene names

Name:	lig
Ordered Locus Names:	MJ0171

Organism

Methanocaldococcus jannaschii (Methanococcus jannaschii) [Complete proteome] [HAMAP]

Taxonomic identifier

2190 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	573 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair By similarity. HAMAP MF_00407
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). HAMAP MF_00407
Cofactor	Divalent metal cations By similarity. HAMAP MF_00407
Sequence similarities	Belongs to the ATP-dependent DNA ligase family.

Ontologies

Keywords
Biological process	Cell cycle Cell division DNA damage DNA recombination DNA repair DNA replication
Ligand	ATP-binding Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: HAMAP DNA repair Inferred from electronic annotation. Source: HAMAP DNA replication Inferred from electronic annotation. Source: HAMAP cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: InterPro DNA ligase (ATP) activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 573	573	DNA ligase HAMAP MF_00407		PRO_0000059604
Sites
	Active site	250	1	N6-AMP-lysine intermediate By similarity
	Binding site	248	1	ATP By similarity
	Binding site	255	1	ATP By similarity
	Binding site	270	1	ATP By similarity
	Binding site	299	1	ATP By similarity
	Binding site	340	1	ATP By similarity
	Binding site	432	1	ATP By similarity
	Binding site	438	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q57635-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 6F95434EE968038C	FASTA	573	66,790
	10 20 30 40 50 60 MLWRDVCEIF NKIEKTTKRL EKRDYFIKLI DMVKEKGKPE DLKKICYMAI GRVYPEYDER 70 80 90 100 110 120 ELGIGEKLLI NAVTSIGIKK DELLEKIKET GDIGLAIEQL KSKIKQASLF FQPLTVDEVY 130 140 150 160 170 180 ETLKRVGEIE GEGSQKKKLR LISSLFLRAS PIECRYLARL ILEDMRIGMN VPTILDALSV 190 200 210 220 230 240 YFNVPKEKLE KIYAITNDIG LLAEKLLMGD LESEELKLKL FRPIKPMLAQ LTPSIEEALL 250 260 270 280 290 300 EMGRAQFETK YDGARVQIHK DGNKVKIYSR RLEDVTNALP EIVEAVKNIN VDKLIVEGEC 310 320 330 340 350 360 VAIDKQTGKP RPFQDILRRF RRKYDIGKMM KEINLRVYLF DILYKDGVSF IDEEFEKRRK 370 380 390 400 410 420 VLEEIVGYEN DWRTERKRIE KELKSDKIID ISYKLVTNDA KEAREFYNWS LSIGHEGVMI 430 440 450 460 470 480 KNLKAPYTPG SRVRTMYKFK PTLESLDVVI TKAKRGMGKR KDWYGSFEIC VRDEEGNLYP 490 500 510 520 530 540 IGHVGTGLTE ADLEFLKEEI DKIIIRDLGE EVEVEPKIVI EVAYEEIQKS DKYPCGYALR 550 560 570 FPRVVRFRFD KGVNEINTIE DVERIYEIQR GRK

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L77117 Genomic DNA. Translation: AAB98156.1.
PIR	D64321.
RefSeq	NP_247139.1.
3D structure databases
SMR	Q57635. Positions 1-572.
ModBase	Search...
Genome annotation databases
GeneID	1451018.
GenomeReviews	Gene locus MJ0171 in contig L77117_GR.
KEGG	mja:MJ0171.
NMPDR	fig|243232.1.peg.176.
TIGR	MJ0171.
Phylogenomic databases
HOGENOM	HBG570821.
OMA	GRPRPFQ.
Enzyme and pathway databases
BRENDA	6.5.1.1. 256362.
Family and domain databases
HAMAP	MF_00407. DNA_ligase. [Tree]
InterPro	IPR000977. DNA_ligase. IPR012309. DNA_ligase_A_C. IPR012310. DNA_ligase_A_M. IPR016059. DNA_ligase_CS. IPR012340. NA-bd_OB-fold. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR00574. dnl1. 1 hit.
PROSITE	PS00697. DNA_LIGASE_A1. 1 hit. PS00333. DNA_LIGASE_A2. 1 hit. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI_METJA

Accession

Primary (citable) accession number: Q57635

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	February 9, 2010
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)