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Protein

DNA ligase

Gene

lig

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.UniRule annotation

Catalytic activityi

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei248 – 2481ATPUniRule annotation
Active sitei250 – 2501N6-AMP-lysine intermediateUniRule annotation
Binding sitei255 – 2551ATPUniRule annotation
Binding sitei270 – 2701ATPUniRule annotation
Binding sitei299 – 2991ATPUniRule annotation
Binding sitei340 – 3401ATPUniRule annotation
Binding sitei432 – 4321ATPUniRule annotation
Binding sitei438 – 4381ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. DNA binding Source: InterPro
  3. DNA ligase (ATP) activity Source: UniProtKB-HAMAP
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. cell division Source: UniProtKB-KW
  3. DNA biosynthetic process Source: InterPro
  4. DNA ligation involved in DNA repair Source: InterPro
  5. DNA recombination Source: UniProtKB-HAMAP
  6. DNA replication Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Cell cycle, Cell division, DNA damage, DNA recombination, DNA repair, DNA replication

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.5.1.1. 3260.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligaseUniRule annotation (EC:6.5.1.1UniRule annotation)
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP]UniRule annotation
Gene namesi
Name:ligUniRule annotation
Ordered Locus Names:MJ0171
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
ProteomesiUP000000805 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 573573DNA ligasePRO_0000059604Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi243232.MJ0171.

Structurei

3D structure databases

ProteinModelPortaliQ57635.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATP-dependent DNA ligase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1793.
InParanoidiQ57635.
KOiK10747.
OMAiGRPRPFQ.
PhylomeDBiQ57635.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPiMF_00407. DNA_ligase.
InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q57635-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLWRDVCEIF NKIEKTTKRL EKRDYFIKLI DMVKEKGKPE DLKKICYMAI
60 70 80 90 100
GRVYPEYDER ELGIGEKLLI NAVTSIGIKK DELLEKIKET GDIGLAIEQL
110 120 130 140 150
KSKIKQASLF FQPLTVDEVY ETLKRVGEIE GEGSQKKKLR LISSLFLRAS
160 170 180 190 200
PIECRYLARL ILEDMRIGMN VPTILDALSV YFNVPKEKLE KIYAITNDIG
210 220 230 240 250
LLAEKLLMGD LESEELKLKL FRPIKPMLAQ LTPSIEEALL EMGRAQFETK
260 270 280 290 300
YDGARVQIHK DGNKVKIYSR RLEDVTNALP EIVEAVKNIN VDKLIVEGEC
310 320 330 340 350
VAIDKQTGKP RPFQDILRRF RRKYDIGKMM KEINLRVYLF DILYKDGVSF
360 370 380 390 400
IDEEFEKRRK VLEEIVGYEN DWRTERKRIE KELKSDKIID ISYKLVTNDA
410 420 430 440 450
KEAREFYNWS LSIGHEGVMI KNLKAPYTPG SRVRTMYKFK PTLESLDVVI
460 470 480 490 500
TKAKRGMGKR KDWYGSFEIC VRDEEGNLYP IGHVGTGLTE ADLEFLKEEI
510 520 530 540 550
DKIIIRDLGE EVEVEPKIVI EVAYEEIQKS DKYPCGYALR FPRVVRFRFD
560 570
KGVNEINTIE DVERIYEIQR GRK
Length:573
Mass (Da):66,790
Last modified:November 1, 1996 - v1
Checksum:i6F95434EE968038C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB98156.1.
PIRiD64321.
RefSeqiNP_247139.1. NC_000909.1.

Genome annotation databases

EnsemblBacteriaiAAB98156; AAB98156; MJ_0171.
GeneIDi1451018.
KEGGimja:MJ_0171.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB98156.1.
PIRiD64321.
RefSeqiNP_247139.1. NC_000909.1.

3D structure databases

ProteinModelPortaliQ57635.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243232.MJ0171.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB98156; AAB98156; MJ_0171.
GeneIDi1451018.
KEGGimja:MJ_0171.

Phylogenomic databases

eggNOGiCOG1793.
InParanoidiQ57635.
KOiK10747.
OMAiGRPRPFQ.
PhylomeDBiQ57635.

Enzyme and pathway databases

BRENDAi6.5.1.1. 3260.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPiMF_00407. DNA_ligase.
InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Entry informationi

Entry nameiDNLI_METJA
AccessioniPrimary (citable) accession number: Q57635
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: April 1, 2015
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.