Reviewed, UniProtKB/Swiss-Prot Q57617 (ACDA_METJA)

Names and origin

Protein names

Recommended name:
    Acetyl-CoA decarbonylase/synthase complex subunit alpha
      Short name=ACDS complex subunit alpha
    EC=1.2.99.2
Alternative name(s):
    ACDS complex carbon monoxide dehydrogenase
      Short name=ACDS CODH

Gene names

Name:	cdhA
Ordered Locus Names:	MJ0153

Organism

Methanocaldococcus jannaschii (Methanococcus jannaschii) [Complete proteome] [HAMAP]

Taxonomic identifier

2190 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	774 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Part of a complex that catalyzes the reversible cleavage of acetyl-CoA, allowing autotrophic growth from CO2 By similarity.
Catalytic activity	CO + H2O + A = CO2 + AH2. HAMAP MF_01137
Cofactor	Binds 7 4Fe-4S clusters per heterotetramer Potential. Binds 2 nickel-iron-sulfur clusters per heterotetramer Potential.
Subunit structure	Heterotetramer of two alpha and two epsilon chains. The ACDS complex is made up of alpha, epsilon, beta, gamma and delta chains with a probable stoichiometry of (alpha2epsilon2)(4)-beta(8)-(gamma1delta1)8 Potential.
Domain	Cluster B is an all-cysteinyl-liganded 4Fe4S cluster; cluster C is a mixed Ni-Fe-S cluster which appears to be the active site of CO oxidation. Cluster D is also an all-cysteinyl-liganded 4Fe4S cluster that bridges the two subunits of the CODH dimer. May contain two additional 4Fe-4S clusters, dubbed E and F, that might reroute electron transfer along different paths. HAMAP MF_01137
Sequence similarities	Belongs to the Ni-containing carbon monoxide dehydrogenase family. Contains 2 4Fe-4S ferredoxin-type domains.

Ontologies

Keywords
Domain	Repeat
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding Nickel
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	acetyl-CoA metabolic process Inferred from electronic annotation. Source: InterPro generation of precursor metabolites and energy Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW carbon-monoxide dehydrogenase (acceptor) activity Inferred from electronic annotation. Source: HAMAP electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW nickel ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 774	774	Acetyl-CoA decarbonylase/synthase complex subunit alpha HAMAP MF_01137		PRO_0000155077
Regions
	Domain	398 – 427	30	4Fe-4S ferredoxin-type 1
	Domain	436 – 466	31	4Fe-4S ferredoxin-type 2
Sites
	Metal binding	73	1	Iron-sulfur 1 (4Fe-4S); shared with dimeric partner By similarity
	Metal binding	76	1	Iron-sulfur 1 (4Fe-4S); shared with dimeric partner By similarity
	Metal binding	77	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	79	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	84	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	94	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	251	1	Nickel-iron-sulfur By similarity
	Metal binding	279	1	Nickel-iron-sulfur By similarity
	Metal binding	318	1	Nickel-iron-sulfur By similarity
	Metal binding	408	1	Iron-sulfur 3 (4Fe-4S) Potential
	Metal binding	411	1	Iron-sulfur 3 (4Fe-4S) Potential
	Metal binding	414	1	Iron-sulfur 3 (4Fe-4S) Potential
	Metal binding	418	1	Iron-sulfur 3 (4Fe-4S) Potential
	Metal binding	446	1	Iron-sulfur 4 (4Fe-4S) Potential
	Metal binding	449	1	Iron-sulfur 4 (4Fe-4S) Potential
	Metal binding	452	1	Iron-sulfur 4 (4Fe-4S) Potential
	Metal binding	456	1	Iron-sulfur 4 (4Fe-4S) Potential
	Metal binding	514	1	Nickel-iron-sulfur By similarity
	Metal binding	543	1	Nickel-iron-sulfur By similarity
	Metal binding	578	1	Nickel-iron-sulfur By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q57617-1 [UniParc]. Last modified December 15, 1998. Version 2. Checksum: F7CFD66F15CB1C7E	FASTA	774	85,900
	10 20 30 40 50 60 MVMGNNVEMD IKKLLTPLVK MKNANISMSI KFGEEEEEEW EPMGPTPMPK IPTLRHWDFK 70 80 90 100 110 120 LLERYPPFYM PICDLCCLCT FGKCDLSRGK KGACGLNIKA QQARIVLIAC CIGAACHAGH 130 140 150 160 170 180 SRHLVHHLIE TLGRDYPIDL GNEIEVEAPI ARTVTGIKPK TLGDLEKILD YCEEQITHLL 190 200 210 220 230 240 SAAHTGQEGD YLDFESKALH AGMIDDLARE AGDLAQIVAY NMPKGDEDAP LIELGFGCID 250 260 270 280 290 300 KSKPVILCIG HNVVPGSYIL EYLEENSMED EVEVCGICCT AIDITRVSDK PKVVGPLSRQ 310 320 330 340 350 360 LMFVRSGVAD VVIVDEQCIR TDILEEVLKT GAVLIATNEK MCLGLEDVSH MDEDEIIGYL 370 380 390 400 410 420 LRNRAALLLD EKKVGKVAVE VAKIVAKERK DRKTLPDLNE VVELAKQCTE CGWCNRNCPN 430 440 450 460 470 480 AFKVKEAMAL AKQGNFKGFI DLYKRCYGCG RCEAICPRNL PIVSMTTKVG EAYYKDLKFK 490 500 510 520 530 540 MRAGRGPIKD VEIRSVGAPI VFGDIPGVVA LVGCSNHPNG EEEVAMIAKE FLERKYIVVA 550 560 570 580 590 600 TGCAAMAIGM WKDKDGKTLY EKYPGEFRAG GLVNCGSCLS NCHITGAAIK IANIFAKVPL 610 620 630 640 650 660 RGNYAEVADY ILNKVGAVGV AWGAMSQKAA AIATGVNRWG IPVILGPHGA KYRRLYLSNG 670 680 690 700 710 720 EKFKVKDKKT GEILEIEPAP EHLIVTAENV KECICMIPKL CMRPNDTPKG RANKIYHYVD 730 740 750 760 770 VYEKYFGRMP PDLEKFVRTE KDIPFMMKDK IMAYLEEKGW KPLEKYPQDP TILY

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L77117 Genomic DNA. Translation: AAB98135.1.
PIR	B64319.
RefSeq	NP_247121.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1450997.
GenomeReviews	Gene locus MJ0153 in contig L77117_GR.
KEGG	mja:MJ0153.
NMPDR	fig|243232.1.peg.158.
TIGR	MJ0153.
Phylogenomic databases
HOGENOM	Q57617.
OMA	Q57617. ICCTAID.
Enzyme and pathway databases
BioCyc	MJAN243232:MJ_0153-MON.
BRENDA	1.2.99.2. 256362.
Family and domain databases
HAMAP	MF_01137. [Tree]
InterPro	IPR017896. 4Fe4S_Fe-S-bd. IPR001450. 4Fe4S_Fe_S_bd_subgr. IPR017900. 4Fe4S_Fe_S_CS. IPR004460. CO_DH/Ac-CoA_synth_asu. IPR016101. CO_DH_a-bundle. IPR004137. Prismane. IPR016099. Prismane-like_a/b-sand. [Graphical view]
Gene3D	G3DSA:1.20.1270.30. CO_DH_a-bundle. 1 hit. G3DSA:3.40.50.2030. Prismane-like_a/b-sand. 2 hits.
Pfam	PF00037. Fer4. 1 hit. PF03063. Prismane. 1 hit. [Graphical view]
TIGRFAMs	TIGR00314. cdhA. 1 hit.
PROSITE	PS00198. 4FE4S_FER_1. 2 hits. PS51379. 4FE4S_FER_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACDA_METJA

Accession

Primary (citable) accession number: Q57617

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	December 15, 1998
Last modified:	June 16, 2009
This is version 61 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)