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Q57612 (TRUB_METJA) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Probable tRNA pseudouridine synthase B
EC=5.4.99.25
Alternative name(s):
tRNA pseudouridine(55) synthase
Short name=Psi55 synthase
tRNA pseudouridylate synthase
tRNA-uridine isomerase
Gene names
Name:truB
Ordered Locus Names:MJ0148
OrganismMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifier243232 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length336 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Could be responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs By similarity. HAMAP MF_01081

Catalytic activity

tRNA uridine(55) = tRNA pseudouridine(55). HAMAP MF_01081

Sequence similarities

Belongs to the pseudouridine synthase TruB family. Type 2 subfamily.

Contains 1 PUA domain.

Ontologies

Keywords
   Biological processtRNA processing
   Molecular functionIsomerase
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological processpseudouridine synthesis

Inferred from electronic annotation. Source: InterPro

tRNA processing

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionRNA binding

Inferred from electronic annotation. Source: InterPro

pseudouridine synthase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 336336Probable tRNA pseudouridine synthase B HAMAP MF_01081
PRO_0000121959

Regions

Domain248 – 32376PUA

Sites

Active site811Nucleophile By similarity

Secondary structure

1 ....................................................... 336
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q57612 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: F29E3EC979A5A89A

FASTA33638,483
        10         20         30         40         50         60 
MILLEKTQEK KINDKEELIV KEEVETNWDY GCNPYERKIE DLIKYGVVVV DKPRGPTSHE 

        70         80         90        100        110        120 
VSTWVKKILN LDKAGHGGTL DPKVTGVLPV ALERATKTIP MWHIPPKEYV CLMHLHRDAS 

       130        140        150        160        170        180 
EEDILRVFKE FTGRIYQRPP LKAAVKRRLR IRKIHELELL DKDGKDVLFR VKCQSGTYIR 

       190        200        210        220        230        240 
KLCEDIGEAL GTSAHMQELR RTKSGCFEEK DAVYLQDLLD AYVFWKEDGD EEELRRVIKP 

       250        260        270        280        290        300 
MEYGLRHLKK VVVKDSAVDA ICHGADVYVR GIAKLSKGIG KGETVLVETL KGEAVAVGKA 

       310        320        330 
LMNTKEILNA DKGVAVDVER VYMDRGTYPR MWKRKK

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		L77117 Genomic DNA. Translation: AAB98132.1.
PIRE64318.
RefSeqNP_247116.1. NC_000909.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2APOX-ray1.95A1-336[»]
ProteinModelPortalQ57612.
SMRQ57612. Positions 17-331.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1450992.
GenomeReviewsGene locus MJ0148 in contig L77117_GR.
KEGGmja:MJ_0148.
NMPDRfig|243232.1.peg.153.
TIGRMJ0148.

Phylogenomic databases

HOGENOMHBG397258.
OMAIYQRPPL.
ProtClustDBPRK04270.

Enzyme and pathway databases

BioCycMJAN243232:MJ_0148-MONOMER.

Family and domain databases

HAMAPMF_01081. TruB_arch.
[Tree]
InterProIPR012960. Dyskerin-like.
IPR002501. PsdUridine_synth.
IPR020103. PsdUridine_synth_cat_dom.
IPR004802. Pseudouridine_synthase-related.
IPR002478. PUA.
IPR015947. PUA-like_domain.
IPR004521. Uncharacterised_CHP00451.
[Graphical view]
KOK03177.
PANTHERPTHR23127. PTHR23127. 1 hit.
PfamPF08068. DKCLD. 1 hit.
PF01472. PUA. 1 hit.
PF01509. TruB_N. 1 hit.
[Graphical view]
SMARTSM00359. PUA. 1 hit.
[Graphical view]
SUPFAMSSF55120. PsdUridine_synth_cat_dom. 1 hit.
SSF88697. PUA-like. 1 hit.
TIGRFAMsTIGR00425. CBF5. 1 hit.
TIGR00451. Unchar_dom_2. 1 hit.
PROSITEPS50890. PUA. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRUB_METJA
AccessionPrimary (citable) accession number: Q57612
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: November 16, 2011
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Methanococcus jannaschii

Methanococcus jannaschii: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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