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Protein

Probable tRNA pseudouridine synthase B

Gene

truB

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Could be responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs.UniRule annotation

Catalytic activityi

tRNA uridine(55) = tRNA pseudouridine(55).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei81NucleophileUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

tRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
Probable tRNA pseudouridine synthase BUniRule annotation (EC:5.4.99.25UniRule annotation)
Alternative name(s):
tRNA pseudouridine(55) synthaseUniRule annotation
Short name:
Psi55 synthaseUniRule annotation
tRNA pseudouridylate synthaseUniRule annotation
tRNA-uridine isomeraseUniRule annotation
Gene namesi
Name:truBUniRule annotation
Ordered Locus Names:MJ0148
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
Proteomesi
  • UP000000805 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001219591 – 336Probable tRNA pseudouridine synthase BAdd BLAST336

Interactioni

Protein-protein interaction databases

DIPiDIP-60499N.
STRINGi243232.MJ_0148.

Structurei

Secondary structure

1336
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi18 – 22Combined sources5
Helixi34 – 36Combined sources3
Helixi39 – 44Combined sources6
Beta strandi46 – 52Combined sources7
Beta strandi54 – 56Combined sources3
Helixi58 – 68Combined sources11
Beta strandi74 – 78Combined sources5
Beta strandi85 – 92Combined sources8
Helixi93 – 101Combined sources9
Beta strandi107 – 117Combined sources11
Helixi121 – 131Combined sources11
Beta strandi132 – 136Combined sources5
Beta strandi151 – 163Combined sources13
Beta strandi166 – 173Combined sources8
Helixi179 – 189Combined sources11
Beta strandi194 – 204Combined sources11
Helixi209 – 211Combined sources3
Helixi215 – 226Combined sources12
Helixi232 – 237Combined sources6
Beta strandi238 – 240Combined sources3
Helixi241 – 245Combined sources5
Beta strandi248 – 253Combined sources6
Helixi255 – 257Combined sources3
Helixi258 – 262Combined sources5
Helixi269 – 271Combined sources3
Beta strandi272 – 276Combined sources5
Beta strandi284 – 288Combined sources5
Beta strandi294 – 302Combined sources9
Helixi304 – 309Combined sources6
Beta strandi311 – 321Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2APOX-ray1.95A1-336[»]
ProteinModelPortaliQ57612.
SMRiQ57612.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ57612.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini248 – 323PUAUniRule annotationAdd BLAST76

Sequence similaritiesi

Belongs to the pseudouridine synthase TruB family. Type 2 subfamily.UniRule annotation
Contains 1 PUA domain.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00987. Archaea.
COG0130. LUCA.
InParanoidiQ57612.
KOiK11131.
OMAiGPFKEDE.
PhylomeDBiQ57612.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
HAMAPiMF_01081. TruB_arch. 1 hit.
InterProiIPR012960. Dyskerin-like.
IPR020103. PsdUridine_synth_cat_dom.
IPR002501. PsdUridine_synth_N.
IPR002478. PUA.
IPR015947. PUA-like_domain.
IPR004802. tRNA_PsdUridine_synth_B_fam.
IPR026326. TruB_arch.
IPR032819. TruB_C.
IPR004521. Uncharacterised_CHP00451.
[Graphical view]
PANTHERiPTHR23127. PTHR23127. 1 hit.
PfamiPF08068. DKCLD. 1 hit.
PF01472. PUA. 1 hit.
PF16198. TruB_C_2. 1 hit.
PF01509. TruB_N. 1 hit.
[Graphical view]
SMARTiSM01136. DKCLD. 1 hit.
SM00359. PUA. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00425. CBF5. 1 hit.
TIGR00451. unchar_dom_2. 1 hit.
PROSITEiPS50890. PUA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q57612-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MILLEKTQEK KINDKEELIV KEEVETNWDY GCNPYERKIE DLIKYGVVVV
60 70 80 90 100
DKPRGPTSHE VSTWVKKILN LDKAGHGGTL DPKVTGVLPV ALERATKTIP
110 120 130 140 150
MWHIPPKEYV CLMHLHRDAS EEDILRVFKE FTGRIYQRPP LKAAVKRRLR
160 170 180 190 200
IRKIHELELL DKDGKDVLFR VKCQSGTYIR KLCEDIGEAL GTSAHMQELR
210 220 230 240 250
RTKSGCFEEK DAVYLQDLLD AYVFWKEDGD EEELRRVIKP MEYGLRHLKK
260 270 280 290 300
VVVKDSAVDA ICHGADVYVR GIAKLSKGIG KGETVLVETL KGEAVAVGKA
310 320 330
LMNTKEILNA DKGVAVDVER VYMDRGTYPR MWKRKK
Length:336
Mass (Da):38,483
Last modified:November 1, 1996 - v1
Checksum:iF29E3EC979A5A89A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB98132.1.
PIRiE64318.
RefSeqiWP_010869643.1. NC_000909.1.

Genome annotation databases

EnsemblBacteriaiAAB98132; AAB98132; MJ_0148.
GeneIDi1450992.
KEGGimja:MJ_0148.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB98132.1.
PIRiE64318.
RefSeqiWP_010869643.1. NC_000909.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2APOX-ray1.95A1-336[»]
ProteinModelPortaliQ57612.
SMRiQ57612.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-60499N.
STRINGi243232.MJ_0148.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB98132; AAB98132; MJ_0148.
GeneIDi1450992.
KEGGimja:MJ_0148.

Phylogenomic databases

eggNOGiarCOG00987. Archaea.
COG0130. LUCA.
InParanoidiQ57612.
KOiK11131.
OMAiGPFKEDE.
PhylomeDBiQ57612.

Miscellaneous databases

EvolutionaryTraceiQ57612.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
HAMAPiMF_01081. TruB_arch. 1 hit.
InterProiIPR012960. Dyskerin-like.
IPR020103. PsdUridine_synth_cat_dom.
IPR002501. PsdUridine_synth_N.
IPR002478. PUA.
IPR015947. PUA-like_domain.
IPR004802. tRNA_PsdUridine_synth_B_fam.
IPR026326. TruB_arch.
IPR032819. TruB_C.
IPR004521. Uncharacterised_CHP00451.
[Graphical view]
PANTHERiPTHR23127. PTHR23127. 1 hit.
PfamiPF08068. DKCLD. 1 hit.
PF01472. PUA. 1 hit.
PF16198. TruB_C_2. 1 hit.
PF01509. TruB_N. 1 hit.
[Graphical view]
SMARTiSM01136. DKCLD. 1 hit.
SM00359. PUA. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00425. CBF5. 1 hit.
TIGR00451. unchar_dom_2. 1 hit.
PROSITEiPS50890. PUA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRUB_METJA
AccessioniPrimary (citable) accession number: Q57612
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.