Q57576 (ACDG_METJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetyl-CoA decarbonylase/synthase complex subunit gamma
Short name=ACDS complex subunit gamma
EC=2.1.1.-

Alternative name(s):
ACDS complex methyltransferase
Corrinoid/iron-sulfur component large subunit

Gene names

Name:	cdhE
Ordered Locus Names:	MJ0112

Organism

Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)

Taxonomic identifier

243232 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	488 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Part of a complex that catalyzes the reversible cleavage of acetyl-CoA, allowing autotrophic growth from CO2 By similarity. HAMAP MF_01136
Catalytic activity	Cob(I)amide-protein + CH(3)-H4SPt + H+ = CH(3)-cob(III)amide-protein + H4SPt. HAMAP MF_01136
Cofactor	Corrinoid Probable. Binds 1 4Fe-4S cluster Probable.
Subunit structure	Heterodimer of delta and gamma chains. The ACDS complex is made up of alpha, epsilon, beta, gamma and delta chains with a probable stoichiometry of (alpha2epsilon2)(4)-beta(8)-(gamma1delta1)8 Potential.
Sequence similarities	Contains 1 4Fe-4S ferredoxin-type domain.

Ontologies

Keywords
Ligand	4Fe-4S Cobalt Iron Iron-sulfur Metal-binding
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	acetyl-CoA catabolic process Inferred from electronic annotation. Source: InterPro pteridine-containing compound metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW iron ion binding Inferred from electronic annotation. Source: InterPro methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 488	488	Acetyl-CoA decarbonylase/synthase complex subunit gamma HAMAP MF_01136		PRO_0000155120
Sites
	Metal binding	19	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	22	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	27	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	44	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q57576 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: AE09B3CECD238B5A	FASTA	488	55,027
	10 20 30 40 50 60 MPKKISAMDI YKLLPKTNCK KCGYPSCMAF ATKLLEKEAT IDQCPILNTP KFEKNKKKII 70 80 90 100 110 120 ELISPPVKEV WFGNEEKKAV MGGDEVMYRY QLSFFNPTPI GVDISDELSE EEIKNRAKEI 130 140 150 160 170 180 ENFVFERTGE KLKLDFIVIR NASGDVEKFK KAIEIVEKET KMPICIASLN PEVIKEALKV 190 200 210 220 230 240 VKSKPMVYAA TKETLNDFIK VIKEVKKDVV LVLSSNNVKD LKNMAAKCLA NGIEDLVLEP 250 260 270 280 290 300 HTYPENIAET LDLNVMIRRS AIEKEDKYLG FPILNLPINA YYYALKNECP ISGFFEDKEV 310 320 330 340 350 360 VAKMFEATIA NTLMNRYADA LIMHGMDIWE LMPVLTLRQC IYTDPRKPQA VEPGLYPIGN 370 380 390 400 410 420 PDENSPVILT TNFSLTFYTV TGDFEKDNVT CWLLVMDTGG KAVDVSVAGG QYNGENAKKL 430 440 450 460 470 480 IEETGIADKV SHRIIILPAL AASTRGDIED KTGWTCVVGT RDSSQVGDFL RNNWDKILKE WKEKNQTA

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L77117 Genomic DNA. Translation: AAB98093.1.
PIR	H64313.
RefSeq	NP_247076.1. NC_000909.1.
3D structure databases
ProteinModelPortal	Q57576.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1450953.
GenomeReviews	Gene locus MJ0112 in contig L77117_GR.
KEGG	mja:MJ_0112.
NMPDR	fig|243232.1.peg.113.
TIGR	MJ0112.
Phylogenomic databases
HOGENOM	HBG304431.
OMA	YTDPRKP.
ProtClustDB	PRK04165.
Enzyme and pathway databases
BioCyc	MJAN243232:MJ_0112-MONOMER.
Family and domain databases
HAMAP	MF_01136. CdhE. [Tree]
InterPro	IPR016041. Ac-CoA_synth_d_su_TIM-brl. IPR016218. AcylCoA_decarb/synth_gsu. IPR023427. AcylCoA_decarb/synth_gsu_arc. IPR011005. Dihydropteroate_synth-like. IPR007202. FeS. IPR000489. Pterin-binding. [Graphical view]
Gene3D	G3DSA:3.20.20.20. Dhdropt_synth. 1 hit.
KO	K00197.
Pfam	PF03599. CdhD. 1 hit. PF04060. FeS. 1 hit. [Graphical view]
PIRSF	PIRSF000376. AcCoA_decarb_gamma. 1 hit.
SUPFAM	SSF51717. DHP_synth_like. 1 hit.
PROSITE	PS00198. 4FE4S_FER_1. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACDG_METJA

Accession

Primary (citable) accession number: Q57576

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 21, 2003
Last sequence update:	November 1, 1996
Last modified:	November 16, 2011
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program