Q57573 (SUHB_METJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Inositol-1-monophosphatase
Short name=I-1-Pase
Short name=IMPase
Short name=Inositol-1-phosphatase
EC=3.1.3.25

Gene names

Name:	suhB
Ordered Locus Names:	MJ0109

Organism

Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)

Taxonomic identifier

243232 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	252 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Has a broad specificity, it can act on both D and L isomers of I-1-P, on 2'-AMP, pNPP, beta-glycerol phosphate, glucose 1-P and other compounds.
Catalytic activity	Myo-inositol phosphate + H2O = myo-inositol + phosphate.
Cofactor	Magnesium.
Subunit structure	Homodimer.
Sequence similarities	Belongs to the inositol monophosphatase family.

Ontologies

Keywords
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	inositol monophosphate 1-phosphatase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 252

252

Inositol-1-monophosphatase

PRO_0000142580

Sites

Metal binding

65

1

Magnesium 1

Metal binding

81

1

Magnesium 1

Metal binding

81

1

Magnesium 2

Metal binding

83

1

Magnesium 1; via carbonyl oxygen

Metal binding

84

1

Magnesium 2

Metal binding

201

1

Magnesium 2

Binding site

84

1

Substrate

Binding site

194

1

Substrate

Secondary structure

1

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252

Helix Strand Turn

Details...

Helix

3 – 18

16

Helix

19 – 21

3

Turn

25 – 27

3

Beta strand

30 – 34

5

Turn

35 – 37

3

Beta strand

38 – 41

4

Helix

42 – 55

14

Helix

56 – 58

3

Beta strand

60 – 64

5

Helix

65 – 67

3

Beta strand

68 – 70

3

Beta strand

75 – 84

10

Helix

86 – 90

5

Beta strand

97 – 115

19

Helix

116 – 118

3

Beta strand

120 – 125

6

Turn

126 – 128

3

Beta strand

148 – 152

5

Helix

159 – 165

7

Beta strand

167 – 169

3

Helix

175 – 183

9

Beta strand

188 – 192

5

Helix

199 – 210

12

Turn

211 – 213

3

Beta strand

215 – 217

3

Beta strand

219 – 223

5

Beta strand

237 – 241

5

Helix

242 – 249

8

Sequences

	Sequence		Length	Mass (Da)
	Q57573 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 3ED5B4401075EE6E	FASTA	252	28,578
	10 20 30 40 50 60 MKWDEIGKNI AKEIEKEILP YFGRKDKSYV VGTSPSGDET EIFDKISEDI ALKYLKSLNV 70 80 90 100 110 120 NIVSEELGVI DNSSEWTVVI DPIDGSFNFI NGIPFFAFCF GVFKNNEPYY GLTYEFLTKS 130 140 150 160 170 180 FYEAYKGKGA YLNGRKIKVK DFNPNNIVIS YYPSKKIDLE KLRNKVKRVR IFGAFGLEMC 190 200 210 220 230 240 YVAKGTLDAV FDVRPKVRAV DIASSYIICK EAGALITDEN GDELKFDLNA TDRLNIIVAN 250 SKEMLDIILD LL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.
[2]	"Cloning and expression of the inositol monophosphatase gene from Methanococcus jannaschii and characterization of the enzyme." Chen L., Roberts M.F. Appl. Environ. Microbiol. 64:2609-2615(1998) [PubMed: 9647837] [Abstract] Cited for: CHARACTERIZATION.
[3]	"MJ0109 is an enzyme that is both an inositol monophosphatase and the 'missing' archaeal fructose-1,6-bisphosphatase." Stec B., Yang H., Johnson K.A., Chen L., Roberts M.F. Nat. Struct. Biol. 7:1046-1050(2000) [PubMed: 11062561] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) IN COMPLEX WITH SUBSTRATE AND METAL IONS.
[4]	"Crystal structure and catalytic mechanism of the MJ0109 gene product: a bifunctional enzyme with inositol monophosphatase and fructose 1,6-bisphosphatase activities." Johnson K.A., Chen L., Yang H., Roberts M.F., Stec B. Biochemistry 40:618-630(2001) [PubMed: 11170378] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L77117 Genomic DNA. Translation: AAB98091.1.

PIR

E64313.

RefSeq

NP_247073.1. NC_000909.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1DK4	X-ray	2.60	A/B	1-252	[»]
1G0H	X-ray	2.30	A/B	1-252	[»]
1G0I	X-ray	2.40	A/B	1-252	[»]

ProteinModelPortal

Q57573.

SMR

Q57573. Positions 1-252.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

1450950.

GenomeReviews

Gene locus MJ0109 in contig L77117_GR.

KEGG

mja:MJ_0109.

NMPDR

fig|243232.1.peg.110.

TIGR

MJ0109.

Phylogenomic databases

HOGENOM

HBG730251.

OMA

VDIASSY.

ProtClustDB

PRK12676.

Enzyme and pathway databases

BioCyc

MetaCyc:MONOMER-5062.
MJAN243232:MJ_0109-MONOMER.

Family and domain databases

InterPro

IPR020583. Inositol_monoP_metal-BS.
IPR000760. Inositol_monophosphatase.
IPR020550. Inositol_monophosphatase_CS.
[Graphical view]

KO

K01092.

PANTHER

PTHR20854. Inositol_P. 1 hit.

Pfam

PF00459. Inositol_P. 1 hit.
[Graphical view]

PRINTS

PR00377. IMPHPHTASES.

PROSITE

PS00629. IMP_1. 1 hit.
PS00630. IMP_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

SUHB_METJA

Accession

Primary (citable) accession number: Q57573

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	November 16, 2011
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program