Q57572 (KPYK_METJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pyruvate kinase
Short name=PK
EC=2.7.1.40

Gene names

Ordered Locus Names:

MJ0108

Organism

Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) [Reference proteome] [HAMAP]

Taxonomic identifier

243232 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus › Methanocaldococcus jannaschii

Protein attributes

Sequence length	447 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + pyruvate = ADP + phosphoenolpyruvate.
Cofactor	Magnesium By similarity. Potassium By similarity.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the pyruvate kinase family.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding Potassium Pyruvate
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: InterPro potassium ion binding Inferred from electronic annotation. Source: InterPro pyruvate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 447	447	Pyruvate kinase		PRO_0000112128
Sites
	Metal binding	35	1	Potassium By similarity
	Metal binding	37	1	Potassium By similarity
	Metal binding	61	1	Potassium By similarity
	Metal binding	203	1	Magnesium By similarity
	Metal binding	227	1	Magnesium By similarity
	Binding site	33	1	Substrate By similarity
	Binding site	226	1	Substrate; via amide nitrogen By similarity
	Binding site	227	1	Substrate; via amide nitrogen By similarity
	Binding site	259	1	Substrate By similarity
	Site	201	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q57572 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: EA6C4DC031B8C1A4	FASTA	447	50,490
	10 20 30 40 50 60 MVGMMRKTKI LVTLGPSLEN KLDKAINLID GVRFNMSHAT TDYCEKFLNI LEKNNIAKVM 70 80 90 100 110 120 DLKGIKIRIK EVKLKNKILK MGEKVVIGED IKLNYNIDTI EEGHFILIND GKIKLRVVEK 130 140 150 160 170 180 TDKIIAVVEV GGEIKEGMGV NLPDTRIELP IIDETDLKNI KFAVEKDFEY IALSFVRNKE 190 200 210 220 230 240 DVKELKDIIS EYKGDCEVIS KIETKEGLKN IKEIARESDG VMVARGDLGV EVPIENIPIE 250 260 270 280 290 300 QKNILRIANR YGILSITATQ ILDSMINNPF PTRAEVTDIA NAIYDGTDCL MLSNETTIGK 310 320 330 340 350 360 YPIEAIKVLN KVAKVADEHY EEFGDRVCLE VESIDEGLVY AVYELYKKLN TKLVITPTYS 370 380 390 400 410 420 GRTAKLISKL RINSKIIAPT PNIRTLKRLR LVWGVESCLM EEFDDMEKII NTCREMAKKE 430 440 IGKGIYLITL GHPIGQKKTN TIKVESI

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L77117 Genomic DNA. Translation: AAB98090.1.
PIR	D64313.
RefSeq	NP_247072.1. NC_000909.1.
3D structure databases
ProteinModelPortal	Q57572.
ModBase	Search...
Protein-protein interaction databases
STRING	243232.MJ0108.
Proteomic databases
PRIDE	Q57572.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAB98090; AAB98090; MJ_0108.
GeneID	1450949.
KEGG	mja:MJ_0108.
Phylogenomic databases
eggNOG	COG0469.
KO	K00873.
OMA	IDRICTI.
ProtClustDB	CLSK876074.
Enzyme and pathway databases
UniPathway	UPA00109; UER00188.
Family and domain databases
Gene3D	2.40.33.10. 1 hit. 3.20.20.60. 2 hits. 3.40.1380.20. 1 hit.
InterPro	IPR001697. Pyr_Knase. IPR015813. Pyrv/PenolPyrv_Kinase. IPR011037. Pyrv_Knase-like_insert_dom. IPR015794. Pyrv_Knase_a/b. IPR018209. Pyrv_Knase_AS. IPR015793. Pyrv_Knase_brl. IPR015795. Pyrv_Knase_C. IPR015806. Pyrv_Knase_insert_dom. [Graphical view]
PANTHER	PTHR11817. PTHR11817. 1 hit.
Pfam	PF00224. PK. 1 hit. PF02887. PK_C. 1 hit. [Graphical view]
PRINTS	PR01050. PYRUVTKNASE.
SUPFAM	SSF50800. PK_B_barrel_like. 1 hit. SSF52935. Pyruvate_kinase. 1 hit. SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
TIGRFAMs	TIGR01064. pyruv_kin. 1 hit.
PROSITE	PS00110. PYRUVATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPYK_METJA

Accession

Primary (citable) accession number: Q57572

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program