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Protein

Pyruvate kinase

Gene

MJ0108

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:

Pathway:iglycolysis

This protein is involved in step 5 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (gap)
  2. Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase 2 (apgM2), 2,3-bisphosphoglycerate-independent phosphoglycerate mutase 1 (apgM1)
  4. Enolase (eno)
  5. Pyruvate kinase (MJ0108)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei33 – 331SubstrateBy similarity
Metal bindingi35 – 351PotassiumBy similarity
Metal bindingi37 – 371PotassiumBy similarity
Metal bindingi61 – 611PotassiumBy similarity
Sitei201 – 2011Transition state stabilizerBy similarity
Metal bindingi203 – 2031MagnesiumBy similarity
Binding sitei226 – 2261Substrate; via amide nitrogenBy similarity
Metal bindingi227 – 2271MagnesiumBy similarity
Binding sitei227 – 2271Substrate; via amide nitrogenBy similarity
Binding sitei259 – 2591SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase (EC:2.7.1.40)
Short name:
PK
Gene namesi
Ordered Locus Names:MJ0108
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
ProteomesiUP000000805 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 447447Pyruvate kinasePRO_0000112128Add
BLAST

Proteomic databases

PRIDEiQ57572.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi243232.MJ_0108.

Structurei

3D structure databases

ProteinModelPortaliQ57572.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Phylogenomic databases

eggNOGiCOG0469.
InParanoidiQ57572.
KOiK00873.
OMAiFIEHMCL.
PhylomeDBiQ57572.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q57572-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVGMMRKTKI LVTLGPSLEN KLDKAINLID GVRFNMSHAT TDYCEKFLNI
60 70 80 90 100
LEKNNIAKVM DLKGIKIRIK EVKLKNKILK MGEKVVIGED IKLNYNIDTI
110 120 130 140 150
EEGHFILIND GKIKLRVVEK TDKIIAVVEV GGEIKEGMGV NLPDTRIELP
160 170 180 190 200
IIDETDLKNI KFAVEKDFEY IALSFVRNKE DVKELKDIIS EYKGDCEVIS
210 220 230 240 250
KIETKEGLKN IKEIARESDG VMVARGDLGV EVPIENIPIE QKNILRIANR
260 270 280 290 300
YGILSITATQ ILDSMINNPF PTRAEVTDIA NAIYDGTDCL MLSNETTIGK
310 320 330 340 350
YPIEAIKVLN KVAKVADEHY EEFGDRVCLE VESIDEGLVY AVYELYKKLN
360 370 380 390 400
TKLVITPTYS GRTAKLISKL RINSKIIAPT PNIRTLKRLR LVWGVESCLM
410 420 430 440
EEFDDMEKII NTCREMAKKE IGKGIYLITL GHPIGQKKTN TIKVESI
Length:447
Mass (Da):50,490
Last modified:November 1, 1997 - v1
Checksum:iEA6C4DC031B8C1A4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB98090.1.
PIRiD64313.
RefSeqiNP_247072.1. NC_000909.1.
WP_010869600.1. NC_000909.1.

Genome annotation databases

EnsemblBacteriaiAAB98090; AAB98090; MJ_0108.
GeneIDi1450949.
KEGGimja:MJ_0108.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB98090.1.
PIRiD64313.
RefSeqiNP_247072.1. NC_000909.1.
WP_010869600.1. NC_000909.1.

3D structure databases

ProteinModelPortaliQ57572.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243232.MJ_0108.

Proteomic databases

PRIDEiQ57572.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB98090; AAB98090; MJ_0108.
GeneIDi1450949.
KEGGimja:MJ_0108.

Phylogenomic databases

eggNOGiCOG0469.
InParanoidiQ57572.
KOiK00873.
OMAiFIEHMCL.
PhylomeDBiQ57572.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Entry informationi

Entry nameiKPYK_METJA
AccessioniPrimary (citable) accession number: Q57572
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: July 22, 2015
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.