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Protein

Signal recognition particle 54 kDa protein

Gene

srp54

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi107 – 1148GTPUniRule annotation
Nucleotide bindingi188 – 1925GTPUniRule annotation
Nucleotide bindingi247 – 2504GTPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Ligandi

GTP-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Signal recognition particle 54 kDa proteinUniRule annotation
Short name:
SRP54UniRule annotation
Gene namesi
Name:srp54UniRule annotation
Ordered Locus Names:MJ0101
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
Proteomesi
  • UP000000805 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

  • Note: The SRP-RNC complex is targeted to the cytoplasmic membrane.UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Signal recognition particle

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 451451Signal recognition particle 54 kDa proteinPRO_0000101177Add
BLAST

Proteomic databases

PRIDEiQ57565.

Interactioni

Subunit structurei

Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY. Archaeal SRP consists of a 7S RNA molecule of 300 nucleotides and two protein subunits: SRP54 and SRP19.UniRule annotation

Protein-protein interaction databases

DIPiDIP-46442N.
STRINGi243232.MJ_0101.

Structurei

Secondary structure

1
451
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 1512Combined sources
Beta strandi18 – 203Combined sources
Helixi28 – 4013Combined sources
Helixi45 – 517Combined sources
Helixi53 – 597Combined sources
Beta strandi60 – 623Combined sources
Helixi70 – 8617Combined sources
Beta strandi97 – 993Combined sources
Beta strandi103 – 1064Combined sources
Beta strandi109 – 1124Combined sources
Helixi115 – 12814Combined sources
Beta strandi131 – 1344Combined sources
Helixi143 – 1453Combined sources
Helixi148 – 1536Combined sources
Beta strandi158 – 1603Combined sources
Beta strandi162 – 1643Combined sources
Beta strandi167 – 1693Combined sources
Helixi172 – 1776Combined sources
Beta strandi183 – 1886Combined sources
Helixi196 – 20510Combined sources
Beta strandi208 – 2103Combined sources
Beta strandi213 – 2208Combined sources
Helixi221 – 2266Combined sources
Helixi227 – 2359Combined sources
Beta strandi242 – 2476Combined sources
Beta strandi249 – 2513Combined sources
Helixi256 – 2649Combined sources
Beta strandi269 – 2724Combined sources
Beta strandi275 – 2806Combined sources
Beta strandi281 – 2833Combined sources
Helixi286 – 2938Combined sources
Turni305 – 3073Combined sources
Turni308 – 3114Combined sources
Beta strandi312 – 3154Combined sources
Helixi318 – 3214Combined sources
Helixi327 – 3348Combined sources
Turni335 – 3373Combined sources
Helixi345 – 3484Combined sources
Helixi351 – 3555Combined sources
Helixi360 – 3634Combined sources
Helixi367 – 37711Combined sources
Helixi382 – 3854Combined sources
Helixi388 – 3903Combined sources
Helixi393 – 40210Combined sources
Helixi407 – 42317Combined sources
Turni424 – 4274Combined sources
Beta strandi434 – 4363Combined sources
Helixi437 – 4459Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2V3CX-ray2.50C/D3-427[»]
3NDBX-ray3.00B3-431[»]
4XCOX-ray2.90C/D304-451[»]
ProteinModelPortaliQ57565.
SMRiQ57565. Positions 2-427.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ57565.

Family & Domainsi

Domaini

Composed of three domains: the N-terminal N domain, which is responsible for interactions with the ribosome, the central G domain, which binds GTP, and the C-terminal M domain, which binds the RNA and the signal sequence of the RNC.UniRule annotation

Sequence similaritiesi

Belongs to the GTP-binding SRP family. SRP54 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01228. Archaea.
COG0541. LUCA.
InParanoidiQ57565.
KOiK03106.
OMAiVICADTF.
PhylomeDBiQ57565.

Family and domain databases

Gene3Di1.10.260.30. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00306. SRP54.
InterProiIPR003593. AAA+_ATPase.
IPR027417. P-loop_NTPase.
IPR013822. Signal_recog_particl_SRP54_hlx.
IPR004125. Signal_recog_particle_SRP54_M.
IPR022941. SRP54.
IPR000897. SRP54_GTPase_dom.
[Graphical view]
PfamiPF00448. SRP54. 1 hit.
PF02881. SRP54_N. 1 hit.
PF02978. SRP_SPB. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00962. SRP54. 1 hit.
SM00963. SRP54_N. 1 hit.
[Graphical view]
SUPFAMiSSF47364. SSF47364. 1 hit.
SSF47446. SSF47446. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00300. SRP54. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q57565-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLDKLGENLN KALNKLKAAA FVDKKLIKEV IKDIQRALIQ ADVNVKLVLK
60 70 80 90 100
MSKEIERRAL EEKTPKGLSK KEHIIKIVYE ELVKLLGEEA KKLELNPKKQ
110 120 130 140 150
NVILLVGIQG SGKTTTAAKL ARYIQKRGLK PALIAADTYR PAAYEQLKQL
160 170 180 190 200
AEKIHVPIYG DETRTKSPVD IVKEGMEKFK KADVLIIDTA GRHKEEKGLL
210 220 230 240 250
EEMKQIKEIT NPDEIILVID GTIGQQAGIQ AKAFKEAVGE IGSIIVTKLD
260 270 280 290 300
GSAKGGGALS AVAETKAPIK FIGIGEGIDD LEPFDPKKFI SRLLGMGDLE
310 320 330 340 350
SLLEKAEDMV DEKTEESIDA IMRGKFTLNE LMTQLEAIEN MGSMKKILSM
360 370 380 390 400
IPGFGGAMPK ELSHLTEAKI KKYKVIISSM TKEERENPKI IKASRIRRIA
410 420 430 440 450
RGSGTTENDV REVLRYYETT KNAIDKLRKG KMLRIGGPLG QIMRQLMFKE

G
Length:451
Mass (Da):50,124
Last modified:November 1, 1997 - v1
Checksum:i5EEAF505FD227914
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB98081.1.
PIRiE64312.

Genome annotation databases

EnsemblBacteriaiAAB98081; AAB98081; MJ_0101.
KEGGimja:MJ_0101.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB98081.1.
PIRiE64312.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2V3CX-ray2.50C/D3-427[»]
3NDBX-ray3.00B3-431[»]
4XCOX-ray2.90C/D304-451[»]
ProteinModelPortaliQ57565.
SMRiQ57565. Positions 2-427.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-46442N.
STRINGi243232.MJ_0101.

Proteomic databases

PRIDEiQ57565.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB98081; AAB98081; MJ_0101.
KEGGimja:MJ_0101.

Phylogenomic databases

eggNOGiarCOG01228. Archaea.
COG0541. LUCA.
InParanoidiQ57565.
KOiK03106.
OMAiVICADTF.
PhylomeDBiQ57565.

Miscellaneous databases

EvolutionaryTraceiQ57565.

Family and domain databases

Gene3Di1.10.260.30. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00306. SRP54.
InterProiIPR003593. AAA+_ATPase.
IPR027417. P-loop_NTPase.
IPR013822. Signal_recog_particl_SRP54_hlx.
IPR004125. Signal_recog_particle_SRP54_M.
IPR022941. SRP54.
IPR000897. SRP54_GTPase_dom.
[Graphical view]
PfamiPF00448. SRP54. 1 hit.
PF02881. SRP54_N. 1 hit.
PF02978. SRP_SPB. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00962. SRP54. 1 hit.
SM00963. SRP54_N. 1 hit.
[Graphical view]
SUPFAMiSSF47364. SSF47364. 1 hit.
SSF47446. SSF47446. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00300. SRP54. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Entry informationi

Entry nameiSRP54_METJA
AccessioniPrimary (citable) accession number: Q57565
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: June 8, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.