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Protein

Thiol peroxidase

Gene

tpx

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides.UniRule annotation1 Publication

Miscellaneous

The active site is a conserved redox-active cysteine residue, the peroxidatic cysteine (C(P)), which makes the nucleophilic attack on the peroxide substrate. The peroxide oxidizes the C(P)-SH to cysteine sulfenic acid (C(P)-SOH), which then reacts with another cysteine residue, the resolving cysteine (C(R)), to form a disulfide bridge. The disulfide is subsequently reduced by an appropriate electron donor to complete the catalytic cycle. In this atypical 2-Cys peroxiredoxin, C(R) is present in the same subunit to form an intramolecular disulfide. The disulfide is subsequently reduced by thioredoxin.UniRule annotation

Catalytic activityi

2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH.UniRule annotation1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei59Cysteine sulfenic acid (-SOH) intermediateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAntioxidant, Oxidoreductase, Peroxidase

Names & Taxonomyi

Protein namesi
Recommended name:
Thiol peroxidaseUniRule annotation (EC:1.11.1.15UniRule annotation1 Publication)
Short name:
TpxUniRule annotation
Alternative name(s):
Peroxiredoxin tpxUniRule annotation
Short name:
PrxUniRule annotation
Scavengase p201 Publication
Thioredoxin peroxidaseUniRule annotation
Gene namesi
Name:tpxUniRule annotation
Ordered Locus Names:HI_0751
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
Proteomesi
  • UP000000579 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001878802 – 165Thiol peroxidaseAdd BLAST164

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi59 ↔ 93Combined sources1 Publication
Disulfide bondi59 ↔ 93Redox-activeUniRule annotation

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi71421.HI0751.

Structurei

Secondary structure

1165
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 5Combined sources3
Beta strandi8 – 10Combined sources3
Beta strandi12 – 14Combined sources3
Beta strandi27 – 29Combined sources3
Beta strandi35 – 37Combined sources3
Helixi38 – 41Combined sources4
Beta strandi44 – 50Combined sources7
Helixi61 – 72Combined sources12
Beta strandi76 – 84Combined sources9
Helixi86 – 89Combined sources4
Turni93 – 97Combined sources5
Beta strandi101 – 105Combined sources5
Helixi111 – 115Combined sources5
Turni124 – 127Combined sources4
Beta strandi132 – 136Combined sources5
Beta strandi140 – 147Combined sources8
Helixi157 – 162Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Q98X-ray1.90A/B1-165[»]
ProteinModelPortaliQ57549.
SMRiQ57549.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ57549.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini17 – 165ThioredoxinUniRule annotationAdd BLAST149

Sequence similaritiesi

Belongs to the peroxiredoxin family. Tpx subfamily.UniRule annotation

Keywords - Domaini

Redox-active center

Phylogenomic databases

eggNOGiENOG4108V1J. Bacteria.
COG2077. LUCA.
KOiK11065.
OMAiKFNAQAN.
PhylomeDBiQ57549.

Family and domain databases

CDDicd03014. PRX_Atyp2cys. 1 hit.
HAMAPiMF_00269. Tpx. 1 hit.
InterProiView protein in InterPro
IPR002065. Put_TPX.
IPR013740. Redoxin.
IPR012336. Thioredoxin-like_fold.
IPR013766. Thioredoxin_domain.
IPR018219. Tpx_CS.
PfamiView protein in Pfam
PF08534. Redoxin. 1 hit.
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiView protein in PROSITE
PS51352. THIOREDOXIN_2. 1 hit.
PS01265. TPX. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q57549-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVTLAGNPI EVGGHFPQVG EIVENFILVG NDLADVALND FASKRKVLNI
60 70 80 90 100
FPSIDTGVCA TSVRKFNQQA AKLSNTIVLC ISADLPFAQA RFCGAEGIEN
110 120 130 140 150
AKTVSTFRNH ALHSQLGVDI QTGPLAGLTS RAVIVLDEQN NVLHSQLVEE
160
IKEEPNYEAA LAVLA
Length:165
Mass (Da):17,626
Last modified:November 1, 1996 - v1
Checksum:i221DA969C76106CB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC22410.1.
PIRiG64090.
RefSeqiNP_438910.1. NC_000907.1.
WP_005693150.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC22410; AAC22410; HI_0751.
GeneIDi949774.
KEGGihin:HI0751.
PATRICifig|71421.8.peg.788.

Similar proteinsi

Entry informationi

Entry nameiTPX_HAEIN
AccessioniPrimary (citable) accession number: Q57549
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: August 30, 2017
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families