Q57532 (GYRA_MYCLE) Reviewed, UniProtKB/Swiss-Prot
Last modified
May 29, 2013.
Version 102.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: DNA gyrase subunit A EC=5.99.1.3 Cleaved into the following chain: | ||||
| Gene names |
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| Organism | Mycobacterium leprae (strain TN) [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 272631 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › ![]() |
Protein attributes
| Sequence length | 1273 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Inferred from homology |
General annotation (Comments)
| Function | DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings By similarity. HAMAP-Rule MF_01897 |
| Catalytic activity | ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP-Rule MF_01897 |
| Subunit structure | Heterotetramer, composed of two GyrA and two GyrB chains. Within the heterotetramer, GyrA contains the active site tyrosine that forms a covalent intermediate with the DNA, while GyrB contributes the cofactor binding sites and catalyzes ATP hydrolysis By similarity. |
| Subcellular location | Cytoplasm Potential HAMAP-Rule MF_01897. |
| Post-translational modification | This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential. HAMAP-Rule MF_01897 |
| Sequence similarities | Belongs to the topoisomerase GyrA/ParC subunit family. Contains 1 DOD-type homing endonuclease domain. |
| Sequence caution | The sequence CAC29514.1 differs from that shown. Reason: Frameshift at position 1247. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cytoplasm |
| Ligand | ATP-binding DNA-binding Nucleotide-binding |
| Molecular function | Isomerase Topoisomerase |
| PTM | Autocatalytic cleavage Protein splicing |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological_process | DNA topological change Inferred from electronic annotation. Source: InterPro intein-mediated protein splicingInferred from electronic annotation. Source: InterPro |
| Cellular_component | chromosome Inferred from electronic annotation. Source: InterPro cytoplasmInferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular_function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type II (ATP-hydrolyzing) activityInferred from electronic annotation. Source: EC endonuclease activityInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 130 | 130 | DNA gyrase subunit A, 1st part By similarity | PRO_0000034807 | |||||
| Chain | 131 – 550 | 420 | Mle gyrA intein By similarity | PRO_0000034808 | |||||
| Chain | 551 – 1273 | 723 | DNA gyrase subunit A, 2nd part By similarity | PRO_0000034809 | |||||
Regions | |||||||||
| Domain | 256 – 396 | 141 | DOD-type homing endonuclease | ||||||
Sites | |||||||||
| Active site | 130 | 1 | O-(5'-phospho-DNA)-tyrosine intermediate By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 267 | 1 | F → S in CAA94713. Ref.1 | ||||||
| Sequence conflict | 267 | 1 | F → S in CAA92430. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Homing events in the gyrA gene of some mycobacteria." Fsihi H., Vincent V., Cole S.T. Proc. Natl. Acad. Sci. U.S.A. 93:3410-3415(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [2] | "Massive gene decay in the leprosy bacillus." Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R., Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E., Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K., Duthoy S. Barrell B.G.Nature 409:1007-1011(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TN. |
| [3] | "Sequences of conserved region in the A subunit of DNA gyrase from nine species of the genus Mycobacterium: phylogenetic analysis and implication for intrinsic susceptibility to quinolones." Guillemin I., Cambau E., Jarlier V. Antimicrob. Agents Chemother. 39:2145-2149(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 75-114. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | Z70722 Genomic DNA. Translation: CAA94713.1. Z68206 Genomic DNA. Translation: CAA92430.1. AL583917 Genomic DNA. Translation: CAC29514.1. Frameshift. X87124 Genomic DNA. Translation: CAA60608.1. |
| PIR | F86909. T10006. |
| RefSeq | NP_301134.1. NC_002677.1. |
3D structure databases | |
| ProteinModelPortal | Q57532. |
| SMR | Q57532. Positions 38-130, 132-237, 494-550. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | 272631.ML0006. |
Protein family/group databases | |
| MEROPS | N10.001. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblBacteria | CAC29514; CAC29514; CAC29514. |
| GeneID | 908154. |
| KEGG | mle:ML0006. |
| PATRIC | 18050092. VBIMycLep78757_0006. |
Organism-specific databases | |
| Leproma | ML0006. |
| CMR | Search... |
Phylogenomic databases | |
| eggNOG | COG1372. |
| HOGENOM | HOG000076278. |
| KO | K02469. |
| ProtClustDB | CLSK701787. |
Family and domain databases | |
| Gene3D | 1.10.268.10. 1 hit. 3.10.28.10. 1 hit. 3.30.1360.40. 1 hit. 3.90.199.10. 2 hits. |
| HAMAP | MF_01897. GyrA. Divergent sequence. |
| InterPro | IPR024946. Arg_repress_C-like. IPR005743. GyrA. IPR006691. GyrA/parC_pinwhl. IPR003586. Hint_dom_C. IPR003587. Hint_dom_N. IPR027434. Homing_endonucl. IPR006142. INTEIN. IPR004042. Intein_endonuc. IPR006141. Intein_splice_site. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013757. Topo_IIA_A_a. IPR013760. Topo_IIA_like_dom. [Graphical view] |
| Pfam | PF03989. DNA_gyraseA_C. 6 hits. PF00521. DNA_topoisoIV. 2 hits. [Graphical view] |
| PRINTS | PR00379. INTEIN. |
| SMART | SM00305. HintC. 1 hit. SM00306. HintN. 1 hit. SM00434. TOP4c. 1 hit. [Graphical view] |
| SUPFAM | SSF55608. SSF55608. 1 hit. SSF56719. Topo_IIA_cen. 2 hits. |
| TIGRFAMs | TIGR01063. gyrA. 1 hit. TIGR01443. intein_Cterm. 1 hit. TIGR01445. intein_Nterm. 1 hit. |
| PROSITE | PS50818. INTEIN_C_TER. 1 hit. PS50819. INTEIN_ENDONUCLEASE. 1 hit. PS50817. INTEIN_N_TER. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | GYRA_MYCLE | ||||||||
| Accession | Primary (citable) accession number: Q57532 Secondary accession number(s): Q50209, Q9CDF2 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| Intein-containing proteins List of intein-containing protein entries |
| SIMILARITY comments Index of protein domains and families |

Clusters with
