Q57532 (GYRA_MYCLE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA gyrase subunit A
EC=5.99.1.3

Cleaved into the following chain:

1. Mle gyrA intein

Gene names

Name:	gyrA
Ordered Locus Names:	ML0006

Organism

Mycobacterium leprae (strain TN) [Complete proteome] [HAMAP]

Taxonomic identifier

272631 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium leprae

Protein attributes

Sequence length	1273 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings By similarity. HAMAP-Rule MF_01897
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP-Rule MF_01897
Subunit structure	Heterotetramer, composed of two GyrA and two GyrB chains. Within the heterotetramer, GyrA contains the active site tyrosine that forms a covalent intermediate with the DNA, while GyrB contributes the cofactor binding sites and catalyzes ATP hydrolysis By similarity.
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_01897.
Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential. HAMAP-Rule MF_01897
Sequence similarities	Belongs to the topoisomerase GyrA/ParC subunit family. Contains 1 DOD-type homing endonuclease domain.
Sequence caution	The sequence CAC29514.1 differs from that shown. Reason: Frameshift at position 1247.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
PTM	Autocatalytic cleavage Protein splicing
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA topological change Inferred from electronic annotation. Source: InterPro intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro
Cellular_component	chromosome Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type II (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC endonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 130	130	DNA gyrase subunit A, 1st part By similarity		PRO_0000034807
	Chain	131 – 550	420	Mle gyrA intein By similarity		PRO_0000034808
	Chain	551 – 1273	723	DNA gyrase subunit A, 2nd part By similarity		PRO_0000034809
Regions
	Domain	256 – 396	141	DOD-type homing endonuclease
Sites
	Active site	130	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Experimental info
	Sequence conflict	267	1	F → S in CAA94713. Ref.1
	Sequence conflict	267	1	F → S in CAA92430. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q57532 [UniParc]. Last modified April 27, 2001. Version 2. Checksum: 4B6FFBB5BD7AEF53	FASTA	1,273	141,218
	10 20 30 40 50 60 MTDITLPPGD GSIQRVEPVD IQQEMQRSYI DYAMSVIVGR ALPEVRDGLK PVHRRVLYAM 70 80 90 100 110 120 LDSGFRPDRS HAKSARSVAE TMGNYHPHGD ASIYDTLVRM AQPWSLRYPL VDGQGNFGSP 130 140 150 160 170 180 GNDPPAAMRY CVSGNSLVRL LFGKSIRIGD IVTGAQFNSD NPIDLKVLDR HGNPVVADYL 190 200 210 220 230 240 FHSGEHQTYT VRTTEGYEIT GTSNHPLLCL VNVGGIPTLL WKLIGEIRSG DYVVLQRIPP 250 260 270 280 290 300 VEFGPADWYS TMEALLFGAF ISGGFVFQDH AGFNSLDRDY FTMVVNAYDT VVGGLRCISS 310 320 330 340 350 360 RITVSGSTLL ELDVYNLIEF KKTRLSGLCG QRSADKLVPD WLWHSPSTVK RAFLQALFEG 370 380 390 400 410 420 EGFSSILSRN IIEISYSTLS ERLAADVQQM LLEFGVVSER YCHTVNEYKV VIANRAQVEM 430 440 450 460 470 480 FFTQVGFGVT KQAKLIRDVV SMSPCVGMDI NCVPGLATFI RKHCDNRWVE EDSFNQHNVD 490 500 510 520 530 540 CVQHWHHHSA EIVGHIADPD IRAIVTDLTD GRFYYARVAS VTDTGIQPVF SLHVDTEDHS 550 560 570 580 590 600 FLTNGFISHN TEARLTPLAM EMLREIDEET VDFISNYDGR VQEPMVLPSR FPNLLANGSG 610 620 630 640 650 660 GIAVGMATNI PPHNLYELAD AVFWCLENHD ADEETMLVAV MERVKGPDFP TAGLIVGSQG 670 680 690 700 710 720 IADAYKTGRG SIRIRGVVEV EEDSRGRTSL VITELPYQVN HDNFITSIAE QVRTGRLAGI 730 740 750 760 770 780 SNVEDQGSDR VGVRIVIEIK RDAVAKVVLN NLYKHTQLQT SFGANMLSIV DGVPRTLRLD 790 800 810 820 830 840 QMICYYVEHQ LDVIVRRTTY RLRKANERAH ILRGLVKALD ALDEVITLIR ASQTVDIARV 850 860 870 880 890 900 GVVELLDIDD IQAQAILDMQ LRRLAALERQ RIIDDLAKIE VEIADLGDIL AKPERRRGII 910 920 930 940 950 960 RNELTEIAEK YGDDRRTRII AVDGDVNDED LIAREEVVVT ITETGYAKRT KTDLYRSQKR 970 980 990 1000 1010 1020 GGKGVQGAGL KQDDIVRHFF VCSTHDWILF FTTQGRVYRA KAYELPEASR TARGQHVANL 1030 1040 1050 1060 1070 1080 LAFQPEERIA QVIQIRSYED APYLVLATRA GLVKKSKLTD FDSNRSGGIV AINLRDNDEL 1090 1100 1110 1120 1130 1140 VGAVLCAADG DLLLVSANGQ SIRFSATDEA LRPMGRATSG VQGMRFNADD RLLSLNVVRE 1150 1160 1170 1180 1190 1200 DTYLLVATSG GYAKRTSIEE YPMQGRGGKG VLTVMYDRRR GSLVGAIVVD EDSELYAITS 1210 1220 1230 1240 1250 1260 GGGVIRTTAR QVRQAGRQTK GVRLMNLGEG DTLLAIARNA EESADGVSVK VMISRSRVLS 1270 FFGSDSNTSP DRT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Homing events in the gyrA gene of some mycobacteria." Fsihi H., Vincent V., Cole S.T. Proc. Natl. Acad. Sci. U.S.A. 93:3410-3415(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Massive gene decay in the leprosy bacillus." Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R., Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E., Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K., Duthoy S. , Feltwell T., Fraser A., Hamlin N., Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S., Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R., Barrell B.G. Nature 409:1007-1011(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TN.
[3]	"Sequences of conserved region in the A subunit of DNA gyrase from nine species of the genus Mycobacterium: phylogenetic analysis and implication for intrinsic susceptibility to quinolones." Guillemin I., Cambau E., Jarlier V. Antimicrob. Agents Chemother. 39:2145-2149(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 75-114.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z70722 Genomic DNA. Translation: CAA94713.1. Z68206 Genomic DNA. Translation: CAA92430.1. AL583917 Genomic DNA. Translation: CAC29514.1. Frameshift. X87124 Genomic DNA. Translation: CAA60608.1.
PIR	F86909. T10006.
RefSeq	NP_301134.1. NC_002677.1.
3D structure databases
ProteinModelPortal	Q57532.
SMR	Q57532. Positions 38-130, 132-237, 494-550.
ModBase	Search...
Protein-protein interaction databases
STRING	272631.ML0006.
Protein family/group databases
MEROPS	N10.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAC29514; CAC29514; CAC29514.
GeneID	908154.
KEGG	mle:ML0006.
PATRIC	18050092. VBIMycLep78757_0006.
Organism-specific databases
Leproma	ML0006.
CMR	Search...
Phylogenomic databases
eggNOG	COG1372.
HOGENOM	HOG000076278.
KO	K02469.
ProtClustDB	CLSK701787.
Family and domain databases
Gene3D	1.10.268.10. 1 hit. 3.10.28.10. 1 hit. 3.30.1360.40. 1 hit. 3.90.199.10. 2 hits.
HAMAP	MF_01897. GyrA. Divergent sequence.
InterPro	IPR024946. Arg_repress_C-like. IPR005743. GyrA. IPR006691. GyrA/parC_pinwhl. IPR003586. Hint_dom_C. IPR003587. Hint_dom_N. IPR027434. Homing_endonucl. IPR006142. INTEIN. IPR004042. Intein_endonuc. IPR006141. Intein_splice_site. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013757. Topo_IIA_A_a. IPR013760. Topo_IIA_like_dom. [Graphical view]
Pfam	PF03989. DNA_gyraseA_C. 6 hits. PF00521. DNA_topoisoIV. 2 hits. [Graphical view]
PRINTS	PR00379. INTEIN.
SMART	SM00305. HintC. 1 hit. SM00306. HintN. 1 hit. SM00434. TOP4c. 1 hit. [Graphical view]
SUPFAM	SSF55608. SSF55608. 1 hit. SSF56719. Topo_IIA_cen. 2 hits.
TIGRFAMs	TIGR01063. gyrA. 1 hit. TIGR01443. intein_Cterm. 1 hit. TIGR01445. intein_Nterm. 1 hit.
PROSITE	PS50818. INTEIN_C_TER. 1 hit. PS50819. INTEIN_ENDONUCLEASE. 1 hit. PS50817. INTEIN_N_TER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GYRA_MYCLE

Accession

Primary (citable) accession number: Q57532
Secondary accession number(s): Q50209, Q9CDF2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	April 27, 2001
Last modified:	May 29, 2013
This is version 102 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program