DNA gyrase subunit A - Mycobacterium leprae

Names and origin

Protein names

Recommended name:
    DNA gyrase subunit A
    EC=5.99.1.3
Cleaved into the following chain:
    1- Recommended name:
            Mle gyrA intein

Gene names

Name:	gyrA
Ordered Locus Names:	ML0006

Organism

Mycobacterium leprae [Complete proteome] [HAMAP]

Taxonomic identifier

1769 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

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Protein attributes

Sequence length	1273 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

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General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings.
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA.
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential.
Sequence similarities	Belongs to the topoisomerase gyrA/parC subunit family. Contains 1 DOD-type homing endonuclease domain.
Sequence caution	The sequence CAC29514.1 differs from that shown. Reason: Frameshift at position 1247.

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Ontologies

Keywords
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
PTM	Autocatalytic cleavage Protein splicing
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC endonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 130

130

DNA gyrase subunit A, 1st part By similarity

PRO_0000034807

Chain

131 – 550

420

Mle gyrA intein By similarity

PRO_0000034808

Chain

551 – 1273

723

DNA gyrase subunit A, 2nd part By similarity

PRO_0000034809

Regions

Domain

256 – 396

141

DOD-type homing endonuclease

Sites

Active site

130

1

O-(5'-phospho-DNA)-tyrosine intermediate By similarity

Experimental info

Sequence conflict

267

1

F → S in CAA94713. Ref.1

Sequence conflict

267

1

F → S in CAA92430. Ref.1

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Sequences

Sequence

Length

Mass (Da)

Tools

Q57532-1 [UniParc].

Last modified April 27, 2001. Version 2.
Checksum: 4B6FFBB5BD7AEF53
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FASTA

1,273

141,218

        10         20         30         40         50         60 
MTDITLPPGD GSIQRVEPVD IQQEMQRSYI DYAMSVIVGR ALPEVRDGLK PVHRRVLYAM 

        70         80         90        100        110        120 
LDSGFRPDRS HAKSARSVAE TMGNYHPHGD ASIYDTLVRM AQPWSLRYPL VDGQGNFGSP 

       130        140        150        160        170        180 
GNDPPAAMRY CVSGNSLVRL LFGKSIRIGD IVTGAQFNSD NPIDLKVLDR HGNPVVADYL 

       190        200        210        220        230        240 
FHSGEHQTYT VRTTEGYEIT GTSNHPLLCL VNVGGIPTLL WKLIGEIRSG DYVVLQRIPP 

       250        260        270        280        290        300 
VEFGPADWYS TMEALLFGAF ISGGFVFQDH AGFNSLDRDY FTMVVNAYDT VVGGLRCISS 

       310        320        330        340        350        360 
RITVSGSTLL ELDVYNLIEF KKTRLSGLCG QRSADKLVPD WLWHSPSTVK RAFLQALFEG 

       370        380        390        400        410        420 
EGFSSILSRN IIEISYSTLS ERLAADVQQM LLEFGVVSER YCHTVNEYKV VIANRAQVEM 

       430        440        450        460        470        480 
FFTQVGFGVT KQAKLIRDVV SMSPCVGMDI NCVPGLATFI RKHCDNRWVE EDSFNQHNVD 

       490        500        510        520        530        540 
CVQHWHHHSA EIVGHIADPD IRAIVTDLTD GRFYYARVAS VTDTGIQPVF SLHVDTEDHS 

       550        560        570        580        590        600 
FLTNGFISHN TEARLTPLAM EMLREIDEET VDFISNYDGR VQEPMVLPSR FPNLLANGSG 

       610        620        630        640        650        660 
GIAVGMATNI PPHNLYELAD AVFWCLENHD ADEETMLVAV MERVKGPDFP TAGLIVGSQG 

       670        680        690        700        710        720 
IADAYKTGRG SIRIRGVVEV EEDSRGRTSL VITELPYQVN HDNFITSIAE QVRTGRLAGI 

       730        740        750        760        770        780 
SNVEDQGSDR VGVRIVIEIK RDAVAKVVLN NLYKHTQLQT SFGANMLSIV DGVPRTLRLD 

       790        800        810        820        830        840 
QMICYYVEHQ LDVIVRRTTY RLRKANERAH ILRGLVKALD ALDEVITLIR ASQTVDIARV 

       850        860        870        880        890        900 
GVVELLDIDD IQAQAILDMQ LRRLAALERQ RIIDDLAKIE VEIADLGDIL AKPERRRGII 

       910        920        930        940        950        960 
RNELTEIAEK YGDDRRTRII AVDGDVNDED LIAREEVVVT ITETGYAKRT KTDLYRSQKR 

       970        980        990       1000       1010       1020 
GGKGVQGAGL KQDDIVRHFF VCSTHDWILF FTTQGRVYRA KAYELPEASR TARGQHVANL 

      1030       1040       1050       1060       1070       1080 
LAFQPEERIA QVIQIRSYED APYLVLATRA GLVKKSKLTD FDSNRSGGIV AINLRDNDEL 

      1090       1100       1110       1120       1130       1140 
VGAVLCAADG DLLLVSANGQ SIRFSATDEA LRPMGRATSG VQGMRFNADD RLLSLNVVRE 

      1150       1160       1170       1180       1190       1200 
DTYLLVATSG GYAKRTSIEE YPMQGRGGKG VLTVMYDRRR GSLVGAIVVD EDSELYAITS 

      1210       1220       1230       1240       1250       1260 
GGGVIRTTAR QVRQAGRQTK GVRLMNLGEG DTLLAIARNA EESADGVSVK VMISRSRVLS 

      1270 
FFGSDSNTSP DRT

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Homing events in the gyrA gene of some mycobacteria." Fsihi H., Vincent V., Cole S.T. Proc. Natl. Acad. Sci. U.S.A. 93:3410-3415(1996) [PubMed: 8622949] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Massive gene decay in the leprosy bacillus." Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R., Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E., Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K., Duthoy S. Barrell B.G. Nature 409:1007-1011(2001) [PubMed: 11234002] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TN.
[3]	"Sequences of conserved region in the A subunit of DNA gyrase from nine species of the genus Mycobacterium: phylogenetic analysis and implication for intrinsic susceptibility to quinolones." Guillemin I., Cambau E., Jarlier V. Antimicrob. Agents Chemother. 39:2145-2149(1995) [PubMed: 8540734] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 75-114.

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Cross-references

Sequence databases
EMBL GenBank DDBJ	Z70722 Genomic DNA. Translation: CAA94713.1. Z68206 Genomic DNA. Translation: CAA92430.1. AL583917 Genomic DNA. Translation: CAC29514.1. Frameshift. X87124 Genomic DNA. Translation: CAA60608.1.
PIR	F86909. T10006.
RefSeq	NP_301134.1.
3D structure databases
SMR	Q57532. Positions 38-130, 131-550, 560-919, 934-1241.
ModBase	Search...
Genome annotation databases
GeneID	908154.
KEGG	mle:ML0006.
Organism-specific databases
Leproma	ML0006.
CMR	Search...
Phylogenomic databases
HOGENOM	HBG635183.
Enzyme and pathway databases
BioCyc	MLEP272631:ML0006-MONOMER.
BRENDA	5.99.1.3. 808.
Family and domain databases
InterPro	IPR005743. GyrA. IPR006691. GyrA/parC_pinwhl. IPR003586. Hedgehog_hint_C. IPR003587. Hedgehog_hint_N. IPR006142. INTEIN. IPR004042. Intein_endonuc. IPR006141. Intein_splicing_site. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013757. Topo_IIA_A_a. IPR013760. Topo_IIA_cen. [Graphical view]
Gene3D	G3DSA:3.90.199.10. Topo_IIA_A/C_ab. 1 hit. G3DSA:1.10.268.10. Topo_IIA_A_a. 1 hit.
Pfam	PF03989. DNA_gyraseA_C. 6 hits. PF00521. DNA_topoisoIV. 2 hits. [Graphical view]
PRINTS	PR00379. INTEIN.
SMART	SM00305. HintC. 1 hit. SM00306. HintN. 1 hit. SM00434. TOP4c. 1 hit. [Graphical view]
TIGRFAMs	TIGR01063. gyrA. 1 hit. TIGR01443. intein_Cterm. 1 hit. TIGR01445. intein_Nterm. 1 hit.
PROSITE	PS50818. INTEIN_C_TER. 1 hit. PS50819. INTEIN_ENDONUCLEASE. 1 hit. PS50817. INTEIN_N_TER. 1 hit. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

GYRA_MYCLE

Accession

Primary (citable) accession number: Q57532
Secondary accession number(s): Q50209, Q9CDF2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	April 27, 2001
Last modified:	February 9, 2010
This is version 74 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Intein-containing proteins

List of intein-containing protein entries

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents