Reviewed, UniProtKB/Swiss-Prot Q57532 (GYRA_MYCLE)

Names and origin

Protein names

Recommended name:
    DNA gyrase subunit A
    EC=5.99.1.3
Cleaved into the following chain:
    1- Recommended name:
            Mle gyrA intein

Gene names

Name:	gyrA
Ordered Locus Names:	ML0006

Organism

Mycobacterium leprae [Complete proteome] [HAMAP]

Taxonomic identifier

1769 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	1273 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings.
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA.
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential.
Sequence similarities	Belongs to the topoisomerase gyrA/parC subunit family. Contains 1 DOD-type homing endonuclease domain.
Sequence caution	The sequence CAC29514.1 differs from that shown. Reason: Frameshift at position 1247.

Ontologies

Keywords
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
PTM	Autocatalytic cleavage Protein splicing
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC endonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 130	130	DNA gyrase subunit A, 1st part By similarity		PRO_0000034807
	Chain	131 – 550	420	Mle gyrA intein By similarity		PRO_0000034808
	Chain	551 – 1273	723	DNA gyrase subunit A, 2nd part By similarity		PRO_0000034809
Regions
	Domain	256 – 396	141	DOD-type homing endonuclease
Sites
	Active site	130	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Experimental info
	Sequence conflict	267	1	F → S in CAA94713. Ref.1
	Sequence conflict	267	1	F → S in CAA92430. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q57532-1 [UniParc]. Last modified April 27, 2001. Version 2. Checksum: 4B6FFBB5BD7AEF53	FASTA	1,273	141,218
	10 20 30 40 50 60 MTDITLPPGD GSIQRVEPVD IQQEMQRSYI DYAMSVIVGR ALPEVRDGLK PVHRRVLYAM 70 80 90 100 110 120 LDSGFRPDRS HAKSARSVAE TMGNYHPHGD ASIYDTLVRM AQPWSLRYPL VDGQGNFGSP 130 140 150 160 170 180 GNDPPAAMRY CVSGNSLVRL LFGKSIRIGD IVTGAQFNSD NPIDLKVLDR HGNPVVADYL 190 200 210 220 230 240 FHSGEHQTYT VRTTEGYEIT GTSNHPLLCL VNVGGIPTLL WKLIGEIRSG DYVVLQRIPP 250 260 270 280 290 300 VEFGPADWYS TMEALLFGAF ISGGFVFQDH AGFNSLDRDY FTMVVNAYDT VVGGLRCISS 310 320 330 340 350 360 RITVSGSTLL ELDVYNLIEF KKTRLSGLCG QRSADKLVPD WLWHSPSTVK RAFLQALFEG 370 380 390 400 410 420 EGFSSILSRN IIEISYSTLS ERLAADVQQM LLEFGVVSER YCHTVNEYKV VIANRAQVEM 430 440 450 460 470 480 FFTQVGFGVT KQAKLIRDVV SMSPCVGMDI NCVPGLATFI RKHCDNRWVE EDSFNQHNVD 490 500 510 520 530 540 CVQHWHHHSA EIVGHIADPD IRAIVTDLTD GRFYYARVAS VTDTGIQPVF SLHVDTEDHS 550 560 570 580 590 600 FLTNGFISHN TEARLTPLAM EMLREIDEET VDFISNYDGR VQEPMVLPSR FPNLLANGSG 610 620 630 640 650 660 GIAVGMATNI PPHNLYELAD AVFWCLENHD ADEETMLVAV MERVKGPDFP TAGLIVGSQG 670 680 690 700 710 720 IADAYKTGRG SIRIRGVVEV EEDSRGRTSL VITELPYQVN HDNFITSIAE QVRTGRLAGI 730 740 750 760 770 780 SNVEDQGSDR VGVRIVIEIK RDAVAKVVLN NLYKHTQLQT SFGANMLSIV DGVPRTLRLD 790 800 810 820 830 840 QMICYYVEHQ LDVIVRRTTY RLRKANERAH ILRGLVKALD ALDEVITLIR ASQTVDIARV 850 860 870 880 890 900 GVVELLDIDD IQAQAILDMQ LRRLAALERQ RIIDDLAKIE VEIADLGDIL AKPERRRGII 910 920 930 940 950 960 RNELTEIAEK YGDDRRTRII AVDGDVNDED LIAREEVVVT ITETGYAKRT KTDLYRSQKR 970 980 990 1000 1010 1020 GGKGVQGAGL KQDDIVRHFF VCSTHDWILF FTTQGRVYRA KAYELPEASR TARGQHVANL 1030 1040 1050 1060 1070 1080 LAFQPEERIA QVIQIRSYED APYLVLATRA GLVKKSKLTD FDSNRSGGIV AINLRDNDEL 1090 1100 1110 1120 1130 1140 VGAVLCAADG DLLLVSANGQ SIRFSATDEA LRPMGRATSG VQGMRFNADD RLLSLNVVRE 1150 1160 1170 1180 1190 1200 DTYLLVATSG GYAKRTSIEE YPMQGRGGKG VLTVMYDRRR GSLVGAIVVD EDSELYAITS 1210 1220 1230 1240 1250 1260 GGGVIRTTAR QVRQAGRQTK GVRLMNLGEG DTLLAIARNA EESADGVSVK VMISRSRVLS 1270 FFGSDSNTSP DRT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Homing events in the gyrA gene of some mycobacteria." Fsihi H., Vincent V., Cole S.T. Proc. Natl. Acad. Sci. U.S.A. 93:3410-3415(1996) [PubMed: 8622949] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Massive gene decay in the leprosy bacillus." Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R., Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E., Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K., Duthoy S. , Feltwell T., Fraser A., Hamlin N., Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S., Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R., Barrell B.G. Nature 409:1007-1011(2001) [PubMed: 11234002] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TN.
[3]	"Sequences of conserved region in the A subunit of DNA gyrase from nine species of the genus Mycobacterium: phylogenetic analysis and implication for intrinsic susceptibility to quinolones." Guillemin I., Cambau E., Jarlier V. Antimicrob. Agents Chemother. 39:2145-2149(1995) [PubMed: 8540734] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 75-114.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z70722 Genomic DNA. Translation: CAA94713.1. Z68206 Genomic DNA. Translation: CAA92430.1. AL583917 Genomic DNA. Translation: CAC29514.1. Frameshift. X87124 Genomic DNA. Translation: CAA60608.1.
PIR	F86909. T10006.
RefSeq	NP_301134.1.
3D structure databases
HSSP	HSSP built from PDB template 1AM2 based on UniProtKB P72065.
SMR	Q57532. Positions 494-550.
ModBase	Search...
Genome annotation databases
GeneID	908154.
GenomeReviews	Gene locus ML0006 in contig AL450380_GR.
KEGG	mle:ML0006.
Organism-specific databases
Leproma	ML0006.
CMR	Search...
Phylogenomic databases
HOGENOM	Q57532.
Enzyme and pathway databases
BioCyc	MLEP272631:ML0006-MON.
BRENDA	5.99.1.3. 808.
Family and domain databases
InterPro	IPR005743. GyrA. IPR006691. GyrA/parC_pinwhl. IPR003586. Hedgehog_hint_C. IPR003587. Hedgehog_hint_N. IPR006142. INTEIN. IPR004042. Intein_endonuc. IPR006141. Intein_splicing_site. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013757. Topo_IIA_A_a. [Graphical view]
Gene3D	G3DSA:3.90.199.10. Topo_IIA_A/C_ab. 1 hit. G3DSA:1.10.268.10. Topo_IIA_A_a. 1 hit.
Pfam	PF03989. DNA_gyraseA_C. 6 hits. PF00521. DNA_topoisoIV. 2 hits. [Graphical view]
PRINTS	PR00379. INTEIN.
ProDom	PD000742. DNA_topoisoIV. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00305. HintC. 1 hit. SM00306. HintN. 1 hit. SM00434. TOP4c. 1 hit. [Graphical view]
TIGRFAMs	TIGR01063. gyrA. 1 hit. TIGR01443. intein_Cterm. 1 hit. TIGR01445. intein_Nterm. 1 hit.
PROSITE	PS50818. INTEIN_C_TER. 1 hit. PS50819. INTEIN_ENDONUCLEASE. 1 hit. PS50817. INTEIN_N_TER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GYRA_MYCLE

Accession

Primary (citable) accession number: Q57532
Secondary accession number(s): Q50209, Q9CDF2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	April 27, 2001
Last modified:	July 28, 2009
This is version 70 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)