Reviewed, UniProtKB/Swiss-Prot Q57471 (TRAM_RHIRD)

Names and origin

Protein names

Recommended name:
    Transcriptional repressor traM

Gene names

Name:

traM

Encoded on

Plasmid pTiA6NC

Organism

Rhizobium radiobacter (Agrobacterium tumefaciens) (Agrobacterium radiobacter)

Taxonomic identifier

358 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	102 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function

Negatively regulates conjugation and the expression of tra genes by antagonizing traR/AAI-dependent activation. TraM may either bind or modify traR or AAI making them unavailable. Alternatively, traM may bind tra promoters preventing traR activation.

Ontologies

Keywords
Biological process	Conjugation Transcription Transcription regulation
Molecular function	Repressor
Technical term	3D-structure Plasmid
Gene Ontology (GO)
Biological process	conjugation Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 102

102

Transcriptional repressor traM

PRO_0000065607

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

102

Helix Strand Turn

Details...

Beta strand

3 – 7

5

Helix

16 – 21

6

Helix

26 – 52

27

Helix

56 – 59

4

Helix

67 – 93

27

Sequences

	Sequence		Length	Mass (Da)
	Q57471-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 78991A239468D7A7	FASTA	102	11,401
	10 20 30 40 50 60 MELEDANVTK KVELRPLIGL TRGLPPTDLE TITIDAIRTH RRLVEKADEL FQALPETYKT 70 80 90 100 GQACGGPQHI RYIEASIEMH AQMSALNTLY SILGFIPKVV VN

References

[1]	"Activity of the Agrobacterium Ti plasmid conjugal transfer regulator TraR is inhibited by the product of the traM gene." Fuqua C., Burbea M., Winans S.C. J. Bacteriol. 177:1367-1373(1995) [PubMed: 7868612] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF242881 Genomic DNA. Translation: AAC28120.1.
U16786 Genomic DNA. Translation: AAA64838.1.

PIR

I39703.

RefSeq

NP_059700.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1RFY	X-ray	1.60	A/B	1-102	[»]
1UPG	X-ray	1.80	A/B	1-102	[»]
1US6	X-ray	1.65	A/B	1-102	[»]

ModBase

Search...

Genome annotation databases

GeneID

1224219.

Family and domain databases

InterPro

IPR015309. Transcript_repress_TraM.
[Graphical view]

Pfam

PF09228. Prok-TraM. 1 hit.
[Graphical view]

PIRSF

PIRSF017930. Transcript_repress_TraM. 1 hit.

ProtoNet

Search...

Entry information

Entry name

TRAM_RHIRD

Accession

Primary (citable) accession number: Q57471

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	November 24, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)