Q57408 (HGP4_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable hemoglobin and hemoglobin-haptoglobin-binding protein 4

Gene names

Ordered Locus Names:

HI_1565/HI_1567

Organism

Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) [Reference proteome] [HAMAP]

Taxonomic identifier

71421 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus › Haemophilus influenzae

Protein attributes

Sequence length	999 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Acts as a receptor for hemoglobin or the hemoglobin/haptoglobin complex of the human host and is required for heme uptake By similarity.
Subcellular location	Cell outer membrane; Peripheral membrane protein By similarity.
Miscellaneous	This protein is subject to phase-variable expression associated with alteration in the length of the CCAA repeat region. This mechanism is called slipped-strand mispairing. Addition or loss of CCAA repeat units would change the reading frame and result in introduction of stop codons downstream of the repeat region. This may be a mechanism of regulation and a way to avoid the immunological response of the host By similarity.
Sequence similarities	Belongs to the TonB-dependent receptor family. Hemoglobin/haptoglobin binding protein subfamily.
Sequence caution	The sequence AAC23213.1 differs from that shown. Reason: Frameshift at positions 49 and 289. The first frameshift is found in the repeats region. The sequence AAC23214.1 differs from that shown. Reason: Frameshift at positions 49 and 289. The first frameshift is found in the repeats region.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell membrane Cell outer membrane Membrane
Domain	Repeat Signal TonB box Transmembrane Transmembrane beta strand
Molecular function	Receptor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cell outer membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	receptor activity Inferred from electronic annotation. Source: InterPro transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 999	975	Probable hemoglobin and hemoglobin-haptoglobin-binding protein 4		PRO_0000034788
Regions
	Repeat	26 – 29	4	1
	Repeat	30 – 33	4	2
	Repeat	34 – 37	4	3
	Repeat	38 – 41	4	4
	Repeat	42 – 45	4	5
	Repeat	46 – 49	4	6
	Region	26 – 49	24	6 X 4 AA tandem repeats of P-T-N-Q
	Motif	58 – 65	8	TonB box
	Motif	982 – 999	18	TonB C-terminal box

Sequences

	Sequence		Length	Mass (Da)
	Q57408 [UniParc]. Last modified August 29, 2001. Version 3. Checksum: DAFCD4EB7000A876	FASTA	999	114,315
	10 20 30 40 50 60 MTNFRLNVLA YSVMLGLTAS VAYAEPTNQP TNQPTNQPTN QPTNQPTNQN SNASEQLEQI 70 80 90 100 110 120 NVLGSDNNND NTPPKIAETV KTASQLKRQQ VQDSRDLVRY ETGVTVVEAG RFGSSGYAIR 130 140 150 160 170 180 GVDENRVAIT VDGLHQAETL SSQGFKELFE GYGNFNNTRN SVEIETLKVA KIAKGADSVK 190 200 210 220 230 240 VGSGSLGGAV LFETKDARDF LTEKDWHIGY KAGYSTADNQ GLNAVTLAGR YQMFDALIMH 250 260 270 280 290 300 SKRHGHELEN YDYKNGRDIQ GKEREKADPY TITKESTLVK FSFSPTENHR FTVASDTYLQ 310 320 330 340 350 360 HSRGHDFSYN LVKTTYINKD EEELRHTNDL TKRKNVSFTY ENYTVTPFWD TLKLSYSQQR 370 380 390 400 410 420 ITTRARTEDY CDGNEKCDSY KNPLGLQLKE GKVVDRNGDP VELKLVEDEQ GQKRHQVVDK 430 440 450 460 470 480 YNNPFSVASG TNNDAFVGKQ LSPSEFWLDC SIFNCDKPVR VYKYQYSNQE PESKEVELNR 490 500 510 520 530 540 TMEINGKKFA TYESNNYRDR YHMILPNSKG YLPLDYKERD LNTKTKQINL DLTKAFTLFE 550 560 570 580 590 600 IENELSYGGV YAKTTKEMVN KAGYYGRNPT WWAERTLGKS LLNGLRTCKE DSSYNGLLCP 610 620 630 640 650 660 RHEPKTSFLI PVETTTKSLY FADNIKLHNM LSVDLGYRYD DIKYQPEYIP GVTPKIADDM 670 680 690 700 710 720 VRELFVPLPP ANGKDWQGNP VYTPEQIRKN AEENIAYIAQ EKRFKKHSYS LGATFDPLNF 730 740 750 760 770 780 LRVQVKYSKG FRTPTSDELY FTFKHPDFTI LPNPNMKPEE AKNQEIALTF HHDWGFFSTN 790 800 810 820 830 840 VFQTKYRQFI DLAYLGSRNL SNSVGGQAQA RDFQVYQNVN VDRAKVKGVE INSRLNIGYF 850 860 870 880 890 900 FEKLDGFNVS YKFTYQRGRL DGNRPMNAIQ PKTSVIGLGY DHKEQRFGAD LYVTHVSAKK 910 920 930 940 950 960 AKDTYNMFYK EQGYKDSAVR WRSDDYTLVD FVTYIKPVKN VTLQFGVYNL TDRKYLTWES 970 980 990 ARSIKPFGTS NLINQGTGAG INRFYSPGRN YKLSAEITF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
[2]	White O., Clayton R.A., Kerlavage A.R., Fleischmann R.D., Peterson J., Hickey E., Dodson R., Gwinn M. Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L42023 Genomic DNA. Translation: AAC23213.1. Sequence problems. L42023 Genomic DNA. Translation: AAC23214.1. Sequence problems.
PIR	A64130.
3D structure databases
ProteinModelPortal	Q57408.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC23213; AAC23213; HI_1565. AAC23214; AAC23214; HI_1567.
Family and domain databases
Gene3D	2.170.130.10. 1 hit. 2.40.170.20. 3 hits.
InterPro	IPR012910. Plug. IPR006970. PT. IPR000531. TonB-dep_rcpt_b-brl. IPR010949. TonB_Hb/transfer/lactofer_rcpt. IPR010917. TonB_rcpt_CS. [Graphical view]
Pfam	PF07715. Plug. 1 hit. PF04886. PT. 1 hit. PF00593. TonB_dep_Rec. 1 hit. [Graphical view]
TIGRFAMs	TIGR01786. TonB-hemlactrns. 1 hit.
PROSITE	PS00430. TONB_DEPENDENT_REC_1. False negative. PS01156. TONB_DEPENDENT_REC_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HGP4_HAEIN

Accession

Primary (citable) accession number: Q57408
Secondary accession number(s): O86244, P96344

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	August 29, 2001
Last modified:	April 3, 2013
This is version 89 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program