Reviewed, UniProtKB/Swiss-Prot Q57346 (UDG_STRPN)

Names and origin

Protein names

Recommended name:
    UDP-glucose 6-dehydrogenase
      Short name=UDP-Glc dehydrogenase
      Short name=UDP-GlcDH
      Short name=UDPGDH
    EC=1.1.1.22

Gene names

Name:	cap3A
Synonyms:	cps3D

Organism

Streptococcus pneumoniae

Taxonomic identifier

1313 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	394 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of UDP-glucuronic acid which is required for capsular hyaluronic acid synthesis. Directly responsible for the transformation of some unencapsulated serotype-3 SP mutants to the encapsulated phenotype.
Catalytic activity	UDP-glucose + 2 NAD+ + H2O = UDP-glucuronate + 2 NADH.
Pathway	Nucleotide-sugar biosynthesis; UDP-glucuronic acid biosynthesis; UDP-glucuronic acid from UDP-glucose: step 1/1.
Sequence similarities	Belongs to the UDP-glucose/GDP-mannose dehydrogenase family.

Ontologies

Keywords
Biological process	Capsule biogenesis/degradation
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro UDP-glucose 6-dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 394	394	UDP-glucose 6-dehydrogenase		PRO_0000074053
Regions
	Nucleotide binding	2 – 19	18	NAD Potential
Sites
	Active site	259	1	By similarity
Experimental info
	Sequence conflict	215	1	R → A in AAC43311. Ref.4
	Sequence conflict	247	1	Y → D in AAC43311. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q57346-1 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: C68671ED88140FC3	FASTA	394	44,697
	10 20 30 40 50 60 MKIAIAGSGY VGLSLAVLLA QHHEVKVIDV IKDKVESINN RKSPIKDEAI EKYLVEKELN 70 80 90 100 110 120 LEASLDPAHV YKDVEYAIIA TPTNYDVDLN QFDTSSVEAA IKTCMEYNDT CTIVIKSTIP 130 140 150 160 170 180 EGYTKEVREK FNTDRIIFSP EFLRESKALY DNLYPSRIVV GTDLDDSELT KRAWQFADLL 190 200 210 220 230 240 KGGAIKEEVP ILVVAFNEAE VAKLFSNTYL ATRVRYFNEI DTYSEVKGLN PKTIIDIVCY 250 260 270 280 290 300 DPRIGSYYNN PSFGYGGYCL PKDTKQLKAS FRDVPENLIT AVVQSNKTRK DYIAGAILAK 310 320 330 340 350 360 QPSVVGIYRL IMKSDSDNFR SSAVKGVMER LDNYGKEIVI YEPTIECDTF MGYRVIKSLD 370 380 390 EFKNISDIVV ANRMNDDLRD IQEKLYTRDL FGRE

References

[1]	"Molecular characterization of cap3A, a gene from the operon required for the synthesis of the capsule of Streptococcus pneumoniae type 3: sequencing of mutations responsible for the unencapsulated phenotype and localization of the capsular cluster on the pneumococcal chromosome." Arrecubieta C., Lopez R., Garcia E. J. Bacteriol. 176:6375-6383(1994) [PubMed: 7929009] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 406 / Type 3.
[2]	"Sequence and transcriptional analysis of a DNA region involved in the production of capsular polysaccharide in Streptococcus pneumoniae type 3." Arrecubieta C., Garcia E., Lopez R. Gene 167:1-7(1995) [PubMed: 8566758] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 406 / Type 3.
[3]	Garcia E., Arrecubieta C., Munoz R., Mollerach M., Lopez R. Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 129 AND 228.
[4]	"Characterization of the cassette containing genes for type 3 capsular polysaccharide biosynthesis in Streptococcus pneumoniae." Dillard J.P., Vandersea M.W., Yother J. J. Exp. Med. 181:973-983(1995) [PubMed: 7869055] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: WU2 / Serotype 3.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z47210 Genomic DNA. Translation: CAA87403.1. Z12159 Genomic DNA. Translation: CAA78147.1. U15171 Genomic DNA. Translation: AAC43311.1.
3D structure databases
HSSP	HSSP built from PDB template 1DLJ based on UniProtKB Q07172.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.1.1.22. 600.
Family and domain databases
InterPro	IPR016040. NAD(P)-bd_dom. IPR017476. Nucleotide_sugar_DH. IPR014027. UDP-Glc/GDP-Man_DH_C. IPR014026. UDP-Glc/GDP-Man_DH_dimer. IPR014028. UDP-Glc/GDP-Man_DH_dimer-bd. IPR001732. UDP-Glc/GDP-Man_DH_N. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:3.40.50.1870. UDP-Glc/GDP-Man_DH_C. 1 hit.
PANTHER	PTHR11374. UDPG_MGDP_DH_Creg. 1 hit.
Pfam	PF00984. UDPG_MGDP_dh. 1 hit. PF03720. UDPG_MGDP_dh_C. 1 hit. PF03721. UDPG_MGDP_dh_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR03026. NDP-sugDHase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

UDG_STRPN

Accession

Primary (citable) accession number: Q57346
Secondary accession number(s): P72519, Q54610

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	May 30, 2000
Last modified:	June 16, 2009
This is version 53 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)