Q57307 (CHOD_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cholesterol oxidase
EC=1.1.3.6

Alternative name(s):
Cholesterol isomerase
EC=5.3.3.1

Gene names

Name:	choD
Ordered Locus Names:	Rv3409c, MT3517

Organism

Mycobacterium tuberculosis [Reference proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	578 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the oxidation and isomerization of cholesterol to cholestenone (4-cholesten-3-one), which is an initial step in the cholesterol degradation process. Required for virulence. Ref.4
Catalytic activity	Cholesterol + O2 = cholest-5-en-3-one + H2O2. Ref.4 A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid. Ref.4
Cofactor	FAD By similarity.
Pathway	Lipid metabolism; steroid biosynthesis.
Disruption phenotype	Cells lacking this gene show attenuated pathogenicity in peritoneal macrophages. Ref.4
Sequence similarities	Belongs to the GMC oxidoreductase family. Highly divergent.

Ontologies

Keywords
Biological process	Cholesterol metabolism Lipid metabolism Steroid metabolism Sterol metabolism Virulence
Ligand	FAD Flavoprotein
Molecular function	Isomerase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cholesterol metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from mutant phenotype Ref.4. Source: MTBBASE steroid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	cholesterol oxidase activity Inferred from direct assay Ref.4. Source: UniProtKB steroid delta-isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 578	578	Cholesterol oxidase		PRO_0000405326
Regions
	Nucleotide binding	11 – 27	17	FAD By similarity
Sites
	Active site	470	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q57307 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: E72695DF493397C5	FASTA	578	63,024
	10 20 30 40 50 60 MKPDYDVLII GSGFGGSVTA LRLTEKGYRV GVLEAGRRFS DEEFAKTSWD LRKFLWAPRL 70 80 90 100 110 120 GCYGIQRIHP LRNVMILAGA GVGGGSLNYA NTLYVPPEPF FADQQWSHIT DWRGELMPHY 130 140 150 160 170 180 QQAQRMLGVV QNPTFTDADR IVKEVADEMG FGDTWVPTPV GVFFGPDGTK TPGKTVPDPY 190 200 210 220 230 240 FGGAGPARTG CLECGCCMTG CRHGAKNTLV KNYLGLAESA GAQVIPMTTV KGFERRSDGL 250 260 270 280 290 300 WEVRTVRTGS WLRRDRRTFT ATQLVLAAGT WGTQHLLFKM RDRGRLPGLS KRLGVLTRTN 310 320 330 340 350 360 SESIVGAATL KVNPDLDLTH GVAITSSIHP TADTHIEPVR YGKGSNAMGL LQTLMTDGSG 370 380 390 400 410 420 PQGTDVPRWR QLLQTASQDP RGTIRMLNPR QWSERTVIAL VMQHLDNSIT TFTKRGKLGI 430 440 450 460 470 480 RWYSSKQGHG EPNPTWIPIG NQVTRRIAAK IDGVAGGTWG ELFNIPLTAH FLGGAVIGDD 490 500 510 520 530 540 PEHGVIDPYH RVYGYPTLYV VDGAAISANL GVNPSLSIAA QAERAASLWP NKGETDRRPP 550 560 570 QGEPYRRLAP IQPAHPVVPA DAPGALRWLP IDPVSNAG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"M.tuberculosis cholesterol oxidase." Av-Gay Y., Lim W., Davies J.E. Submitted (JUL-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[3]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
[4]	"Cholesterol oxidase is required for virulence of Mycobacterium tuberculosis." Brzostek A., Dziadek B., Rumijowska-Galewicz A., Pawelczyk J., Dziadek J. FEMS Microbiol. Lett. 275:106-112(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN CHOLESTEROL DEGRADATION, CATALYTIC ACTIVITY, DISRUPTION PHENOTYPE. Strain: ATCC 25618 / H37Rv.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000516 Genomic DNA. Translation: AAK47855.1. X99343 Genomic DNA. Translation: CAA67723.1. AL123456 Genomic DNA. Translation: CCP46231.1.
PIR	F70736.
RefSeq	NP_217926.1. NC_000962.3. NP_338041.1. NC_002755.2. YP_006516895.1. NC_018143.1.
3D structure databases
ProteinModelPortal	Q57307.
SMR	Q57307. Positions 6-38, 472-524.
ModBase	Search...
Protein-protein interaction databases
STRING	83332.Rv3409c.
Proteomic databases
PRIDE	Q57307.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAK47855; AAK47855; MT3517.
GeneID	13317013. 887502. 922932.
KEGG	mtc:MT3517. mtu:Rv3409c.
PATRIC	18129473. VBIMycTub22151_3832.
Organism-specific databases
TubercuList	Rv3409c.
Phylogenomic databases
eggNOG	COG2303.
HOGENOM	HOG000241700.
KO	K03333.
OMA	DPYHRVW.
ProtClustDB	CLSK872179.
Enzyme and pathway databases
UniPathway	UPA00062.
Family and domain databases
InterPro	IPR006076. FAD-dep_OxRdtase. IPR007867. GMC_OxRtase_C. [Graphical view]
Pfam	PF01266. DAO. 1 hit. PF05199. GMC_oxred_C. 1 hit. [Graphical view]
PROSITE	PS00623. GMC_OXRED_1. False negative. PS00624. GMC_OXRED_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHOD_MYCTU

Accession

Primary (citable) accession number: Q57307
Secondary accession number(s): L0TFA8, Q799Z3, Q7D5K5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 8, 2011
Last sequence update:	November 1, 1996
Last modified:	May 29, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program