Afimbrial adhesin AFA-III precursor - Escherichia coli

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Reviewed, UniProtKB/Swiss-Prot Q57254 (AFAE3_ECOLX)

Last modified February 9, 2010. Version 55. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein names

Recommended name:
Afimbrial adhesin AFA-III

Gene names

Name:	afaE3
Synonyms:	afaE-3

Encoded on

Plasmid pIL1055

Organism

Escherichia coli

Taxonomic identifier

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	160 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Hemagglutinins of uropathogenic E.coli mediate adherence to the upper urinary tract. These adhesins bind to the Dr blood group antigen and also agglutinate human erythrocytes in the presence of D-mannose (mannose-resistant hemagglutination (MRHA)).
Subcellular location	Fimbrium.
Sequence similarities	Belongs to the Dr-adhesin family.

Ontologies

Keywords
Cellular component	Fimbrium
Domain	Signal
Technical term	3D-structure Plasmid
Gene Ontology (GO)
Cellular component	pilus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

21

Potential

Chain

139

Afimbrial adhesin AFA-III

PRO_0000000873

Regions

Region

54

Receptor-binding By similarity

Natural variations

Natural variant

1

A → T in strain: AL847.

Natural variant

1

N → D in strain: AL845 and AL847; chloramphenicol-sensitive.

Secondary structure

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160

Helix Strand Turn

Sequences

Sequence

Length

Mass (Da)

Tools

Q57254-1 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: D5228F367A9D0275
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160

17,075

        10         20         30         40         50         60 
MKKLAIMAAA SMVFAVSSAH AGFTPSGTTG TTKLTVTEEC QVRVGDLTVA KTRGQLTDAA 

        70         80         90        100        110        120 
PIGPVTVQAL GCNARQVALK ADTDNFEQGK FFLISDNNRD KLYVNIRPMD NSAWTTDNGV 

       130        140        150        160 
FYKNDVGSWG GTIGIYVDGQ QTNTPPGNYT LTLTGGYWAK

References

[1]	"Nucleotide sequence of the afimbrial-adhesin-encoding afa-3 gene cluster and its translocation via flanking IS1 insertion sequences." Garcia M.-I., Labigne A., le Bouguenec C.L. J. Bacteriol. 176:7601-7613(1994) [PubMed: 8002584] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: A30 / UPEC.
[2]	"Characterization of plasmid-borne afa-3 gene clusters encoding afimbrial adhesins expressed by Escherichia coli strains associated with intestinal or urinary tract infections." le Bouguenec C.L., Garcia M.-I., Ouin V., Desperrier J.-M., Gounon P., Labigne A. Infect. Immun. 61:5106-5114(1993) [PubMed: 7901162] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION. Strain: A30 / UPEC, AL845 and AL847.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X76688 Genomic DNA. Translation: CAA54121.1.
X69102 Genomic DNA. Translation: CAA48847.1.

PIR

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1RXL	NMR	-	A	22-160	[»]
1USZ	X-ray	3.28	A	23-160	[»]
1UT2	X-ray	3.30	A/B/C/D/E/F/G/H/I	23-160	[»]
2IXQ	NMR	-	B	22-160	[»]
2VER	NMR	-	A	22-160	[»]

ModBase

Family and domain databases

InterPro

IPR021020. Adehsin_Dr_signal_peptide.
IPR006713. Adhesin_Dr.
IPR008966. Adhesion_bac.
[Graphical view]

Gene3D

G3DSA:2.60.40.1570. Adhesin_Dr. 1 hit.

Pfam

PF04619. Adhesin_Dr. 1 hit.
PF12393. Dr_adhesin. 1 hit.
[Graphical view]

ProDom

PD008337. Adhesin_Dr. 1 hit.
[Graphical view] [Entries sharing at least one domain]

ProtoNet

Entry information

Entry name

AFAE3_ECOLX

Accession

Primary (citable) accession number: Q57254

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	February 9, 2010
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents