Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Afimbrial adhesin AFA-III

Gene

afaE3

Organism
Escherichia coli
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Hemagglutinins of uropathogenic E.coli mediate adherence to the upper urinary tract. These adhesins bind to the Dr blood group antigen and also agglutinate human erythrocytes in the presence of D-mannose (mannose-resistant hemagglutination (MRHA)).

Names & Taxonomyi

Protein namesi
Recommended name:
Afimbrial adhesin AFA-III
Gene namesi
Name:afaE3
Synonyms:afaE-3
Encoded oniPlasmid pIL10550 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

Subcellular locationi

GO - Cellular componenti

  • pilus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Fimbrium

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000000087322 – 160Afimbrial adhesin AFA-IIIAdd BLAST139

Interactioni

Protein-protein interaction databases

IntActiQ57254. 1 interactor.

Structurei

Secondary structure

1160
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi22 – 37Combined sources16
Beta strandi39 – 46Combined sources8
Beta strandi50 – 52Combined sources3
Helixi53 – 55Combined sources3
Beta strandi61 – 68Combined sources8
Beta strandi75 – 81Combined sources7
Helixi83 – 85Combined sources3
Beta strandi86 – 89Combined sources4
Beta strandi91 – 95Combined sources5
Beta strandi101 – 111Combined sources11
Beta strandi114 – 117Combined sources4
Beta strandi120 – 125Combined sources6
Beta strandi130 – 139Combined sources10
Beta strandi146 – 159Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1RXLNMR-A38-160[»]
1USZX-ray3.28A21-160[»]
1UT2X-ray3.30A/B/C/D/E/F/G/H/I21-160[»]
2IXQNMR-B38-160[»]
2VERNMR-A38-160[»]
ProteinModelPortaliQ57254.
SMRiQ57254.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ57254.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni22 – 75Receptor-bindingBy similarityAdd BLAST54

Sequence similaritiesi

Belongs to the Dr-adhesin family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.60.40.1570. 1 hit.
InterProiIPR021020. Adehsin_Dr_signal_peptide.
IPR006713. Adhesin_Dr.
IPR008966. Adhesion_dom.
[Graphical view]
PfamiPF04619. Adhesin_Dr. 1 hit.
PF12393. Dr_adhesin. 1 hit.
[Graphical view]
ProDomiPD008337. Adhesin_Dr. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF49401. SSF49401. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q57254-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKLAIMAAA SMVFAVSSAH AGFTPSGTTG TTKLTVTEEC QVRVGDLTVA
60 70 80 90 100
KTRGQLTDAA PIGPVTVQAL GCNARQVALK ADTDNFEQGK FFLISDNNRD
110 120 130 140 150
KLYVNIRPMD NSAWTTDNGV FYKNDVGSWG GTIGIYVDGQ QTNTPPGNYT
160
LTLTGGYWAK
Length:160
Mass (Da):17,075
Last modified:November 1, 1997 - v1
Checksum:iD5228F367A9D0275
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti59A → T in strain: AL847. 1
Natural varianti73N → D in strain: AL845 and AL847; chloramphenicol-sensitive. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76688 Genomic DNA. Translation: CAA54121.1.
X69102 Genomic DNA. Translation: CAA48847.1.
PIRiH55545.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76688 Genomic DNA. Translation: CAA54121.1.
X69102 Genomic DNA. Translation: CAA48847.1.
PIRiH55545.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1RXLNMR-A38-160[»]
1USZX-ray3.28A21-160[»]
1UT2X-ray3.30A/B/C/D/E/F/G/H/I21-160[»]
2IXQNMR-B38-160[»]
2VERNMR-A38-160[»]
ProteinModelPortaliQ57254.
SMRiQ57254.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ57254. 1 interactor.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiQ57254.

Family and domain databases

Gene3Di2.60.40.1570. 1 hit.
InterProiIPR021020. Adehsin_Dr_signal_peptide.
IPR006713. Adhesin_Dr.
IPR008966. Adhesion_dom.
[Graphical view]
PfamiPF04619. Adhesin_Dr. 1 hit.
PF12393. Dr_adhesin. 1 hit.
[Graphical view]
ProDomiPD008337. Adhesin_Dr. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF49401. SSF49401. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAFAE3_ECOLX
AccessioniPrimary (citable) accession number: Q57254
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.