Q57233 (THIM_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hydroxyethylthiazole kinase
EC=2.7.1.50

Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinase
Short name=TH kinase
Short name=Thz kinase

Gene names

Name:	thiM
Ordered Locus Names:	HI_0415

Organism

Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

Taxonomic identifier

71421 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	265 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole. HAMAP MF_00228
Cofactor	Magnesium By similarity. HAMAP MF_00228
Pathway	Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole: step 1/1. HAMAP MF_00228
Sequence similarities	Belongs to the Thz kinase family.

Ontologies

Keywords
Biological process	Thiamine biosynthesis
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW hydroxyethylthiazole kinase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 265	265	Hydroxyethylthiazole kinase HAMAP MF_00228		PRO_0000156936
Sites
	Binding site	43	1	Substrate; via amide nitrogen By similarity
	Binding site	119	1	ATP By similarity
	Binding site	165	1	ATP By similarity
	Binding site	192	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q57233 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: C43B7ABED91A8BD6	FASTA	265	27,733
	10 20 30 40 50 60 MLMQSIYLSK IREQNPLIHN ITNIVAANFS ANGLLALGAS PLMSANVEEM QEVPKISQAL 70 80 90 100 110 120 VINIGTLIGK DREAMLQAGK TANEVGIPVV LDPVGVGATS YRRETIRELL AEVKFALIRG 130 140 150 160 170 180 NAGELAAIAG ETWQAKGVDA GQGEVDLKAV AEKVAQRYGC TVLISGAVDI VSDGTQTATV 190 200 210 220 230 240 HNGTSLFPKV TASGCLLSAV CAAFLAVSEG NYFSATLEAC VAYTIAGECA AQGLTTQVGQ 250 260 FQIRLLDELA ALSPETIGQR GRINE

References

[1]

"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed: 7542800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L42023 Genomic DNA. Translation: AAC22073.1.
PIR	H64151.
RefSeq	NP_438577.1. NC_000907.1.
3D structure databases
ProteinModelPortal	Q57233.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	949516.
GenomeReviews	Gene locus HI_0415 in contig L42023_GR.
KEGG	hin:HI0415.
NMPDR	fig|71421.1.peg.390.
PATRIC	20189383. VBIHaeInf48452_0435.
TIGR	HI_0415.
Phylogenomic databases
HOGENOM	HBG351126.
OMA	AIRGNAG.
ProtClustDB	PRK09355.
Enzyme and pathway databases
BioCyc	HINF71421:HI_0415-MONOMER.
Family and domain databases
HAMAP	MF_00228. Thz_kinase. [Tree]
InterPro	IPR000417. Hyethyz_kinase. [Graphical view]
KO	K00878.
PANTHER	PTHR20857:SF14. Hyethyz_kinase. 1 hit.
Pfam	PF02110. HK. 1 hit. [Graphical view]
PIRSF	PIRSF000513. Thz_kinase. 1 hit.
PRINTS	PR01099. HYETHTZKNASE.
TIGRFAMs	TIGR00694. ThiM. 1 hit.
ProtoNet	Search...

Entry information

Entry name

THIM_HAEIN

Accession

Primary (citable) accession number: Q57233

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	January 25, 2012
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program