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Q571F8

- GLSL_MOUSE

UniProt

Q571F8 - GLSL_MOUSE

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Protein

Glutaminase liver isoform, mitochondrial

Gene
Gls2, Kiaa4146
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH and glutathione production By similarity.UniRule annotation

Catalytic activityi

L-glutamine + H2O = L-glutamate + NH3.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei219 – 2191Substrate By similarity
Binding sitei268 – 2681Substrate By similarity
Binding sitei314 – 3141Substrate By similarity
Binding sitei321 – 3211Substrate By similarity
Binding sitei347 – 3471Substrate By similarity
Binding sitei399 – 3991Substrate By similarity
Binding sitei417 – 4171Substrate; via amide nitrogen By similarity

GO - Molecular functioni

  1. glutaminase activity Source: UniProtKB-EC

GO - Biological processi

  1. glutamine metabolic process Source: InterPro
  2. reactive oxygen species metabolic process Source: Ensembl
  3. regulation of apoptotic process Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Glutaminase liver isoform, mitochondrial (EC:3.5.1.2)
Short name:
GLS
Alternative name(s):
L-glutaminase
L-glutamine amidohydrolase
Gene namesi
Name:Gls2
Synonyms:Kiaa4146
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 10

Organism-specific databases

MGIiMGI:2143539. Gls2.

Subcellular locationi

Mitochondrion By similarity UniRule annotation

GO - Cellular componenti

  1. mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 1414Mitochondrion Reviewed predictionAdd
BLAST
Chaini15 – 602588Glutaminase liver isoform, mitochondrialUniRule annotationPRO_0000011626Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei253 – 2531N6-succinyllysine1 Publication
Modified residuei279 – 2791N6-acetyllysine1 Publication
Modified residuei284 – 2841N6-acetyllysine1 Publication
Modified residuei329 – 3291N6-acetyllysine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ571F8.
PaxDbiQ571F8.
PRIDEiQ571F8.

PTM databases

PhosphoSiteiQ571F8.

Expressioni

Gene expression databases

ArrayExpressiQ571F8.
BgeeiQ571F8.
CleanExiMM_GLS2.
GenevestigatoriQ571F8.

Interactioni

Subunit structurei

Interacts with the PDZ domain of the syntrophin SNTA1. Interacts with the PDZ domain of TAX1BP3 By similarity.

Protein-protein interaction databases

IntActiQ571F8. 1 interaction.
STRINGi10090.ENSMUSP00000047376.

Structurei

3D structure databases

ProteinModelPortaliQ571F8.
SMRiQ571F8. Positions 70-479, 490-572.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati518 – 55134ANK 1UniRule annotationAdd
BLAST
Repeati552 – 58534ANK 2UniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the glutaminase family.
Contains 2 ANK repeats.

Keywords - Domaini

ANK repeat, Repeat, Transit peptide

Phylogenomic databases

eggNOGiCOG2066.
GeneTreeiENSGT00390000010463.
HOGENOMiHOG000216891.
HOVERGENiHBG005856.
InParanoidiQ3TMJ6.
KOiK01425.
OMAiCVKPLTY.
OrthoDBiEOG7S4X5F.
PhylomeDBiQ571F8.
TreeFamiTF313359.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
3.40.710.10. 1 hit.
HAMAPiMF_00313. Glutaminase.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR012338. Beta-lactam/transpept-like.
IPR015868. Glutaminase.
[Graphical view]
PANTHERiPTHR12544. PTHR12544. 1 hit.
PfamiPF12796. Ank_2. 1 hit.
PF04960. Glutaminase. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 2 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF56601. SSF56601. 1 hit.
TIGRFAMsiTIGR03814. Gln_ase. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q571F8-1 [UniParc]FASTAAdd to Basket

« Hide

MRSMRALQNA LSRAGSHGRR GGWGHPSRGP LLGRGVRYYL GEAAAQGRGT    50
PHSHQPQHSD HDASHSGMLP RLGDLLFYTI AEGQERIPIH KFTTALKATG 100
LQTSDPRLQD CMSKMQRMVQ ESSSGGLLDR ELFQKCVSSN IVLLTQAFRK 150
KFVIPDFEEF TGHVDRIFED AKEPTGGKVA AYIPHLAKSN PDLWGVSLCT 200
VDGQRHSVGH TKIPFCLQSC VKPLTYAISV STLGTDYVHK FVGKEPSGLR 250
YNKLSLNEEG IPHNPMVNAG AIVVSSLIKM DCNKAEKFDF VLQYLNKMAG 300
NEFMGFSNAT FQSEKETGDR NYAIGYYLKE KKCFPKGVDM MAALDLYFQL 350
CSVEVTCESG SVMAATLANG GICPITGESV LSAEAVRNTL SLMHSCGMYD 400
FSGQFAFHVG LPAKSAVSGA ILLVVPNVMG MMCLSPPLDK LGNSQRGINF 450
CQKLVSLFNF HNYDNLRHCA RKLDPRREGG EVRNKTVVNL LFAAYSGDVS 500
ALRRFALSAM DMEQKDYDSR TALHVAAAEG HIEVVKFLIE ACKVNPFVKD 550
RWGNIPLDDA VQFNHLEVVK LLQDYHDSYL LSETQAEAAA ETLSKENLES 600
MV 602
Length:602
Mass (Da):66,366
Last modified:August 30, 2005 - v2
Checksum:iBA07D22ADB082F76
GO

Sequence cautioni

The sequence BAD90156.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti312 – 3121Q → K in BAE38445. 1 Publication
Sequence conflicti584 – 5841T → S in AAI41116. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK220231 mRNA. Translation: BAD90156.1. Different initiation.
AK165896 mRNA. Translation: BAE38445.1.
BC141115 mRNA. Translation: AAI41116.1.
CCDSiCCDS24263.1.
RefSeqiNP_001028436.2. NM_001033264.3.
UniGeneiMm.30102.
Mm.447148.

Genome annotation databases

EnsembliENSMUST00000044776; ENSMUSP00000047376; ENSMUSG00000044005.
GeneIDi216456.
KEGGimmu:216456.
UCSCiuc007hlo.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK220231 mRNA. Translation: BAD90156.1 . Different initiation.
AK165896 mRNA. Translation: BAE38445.1 .
BC141115 mRNA. Translation: AAI41116.1 .
CCDSi CCDS24263.1.
RefSeqi NP_001028436.2. NM_001033264.3.
UniGenei Mm.30102.
Mm.447148.

3D structure databases

ProteinModelPortali Q571F8.
SMRi Q571F8. Positions 70-479, 490-572.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q571F8. 1 interaction.
STRINGi 10090.ENSMUSP00000047376.

Chemistry

ChEMBLi CHEMBL2176860.

PTM databases

PhosphoSitei Q571F8.

Proteomic databases

MaxQBi Q571F8.
PaxDbi Q571F8.
PRIDEi Q571F8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000044776 ; ENSMUSP00000047376 ; ENSMUSG00000044005 .
GeneIDi 216456.
KEGGi mmu:216456.
UCSCi uc007hlo.2. mouse.

Organism-specific databases

CTDi 27165.
MGIi MGI:2143539. Gls2.
Rougei Search...

Phylogenomic databases

eggNOGi COG2066.
GeneTreei ENSGT00390000010463.
HOGENOMi HOG000216891.
HOVERGENi HBG005856.
InParanoidi Q3TMJ6.
KOi K01425.
OMAi CVKPLTY.
OrthoDBi EOG7S4X5F.
PhylomeDBi Q571F8.
TreeFami TF313359.

Miscellaneous databases

NextBioi 375178.
PROi Q571F8.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q571F8.
Bgeei Q571F8.
CleanExi MM_GLS2.
Genevestigatori Q571F8.

Family and domain databases

Gene3Di 1.25.40.20. 1 hit.
3.40.710.10. 1 hit.
HAMAPi MF_00313. Glutaminase.
InterProi IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR012338. Beta-lactam/transpept-like.
IPR015868. Glutaminase.
[Graphical view ]
PANTHERi PTHR12544. PTHR12544. 1 hit.
Pfami PF12796. Ank_2. 1 hit.
PF04960. Glutaminase. 1 hit.
[Graphical view ]
SMARTi SM00248. ANK. 2 hits.
[Graphical view ]
SUPFAMi SSF48403. SSF48403. 1 hit.
SSF56601. SSF56601. 1 hit.
TIGRFAMsi TIGR03814. Gln_ase. 1 hit.
PROSITEi PS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O., Koga H.
    Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Spleen.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.
  4. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-253, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  5. "Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
    Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
    Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-279; LYS-284 AND LYS-329, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiGLSL_MOUSE
AccessioniPrimary (citable) accession number: Q571F8
Secondary accession number(s): B2RUF2, Q3TMJ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: August 30, 2005
Last modified: July 9, 2014
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi