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Protein

Glutaminase liver isoform, mitochondrial

Gene

Gls2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH and glutathione production (By similarity).By similarity

Catalytic activityi

L-glutamine + H2O = L-glutamate + NH3.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei219 – 2191SubstrateBy similarity
Binding sitei268 – 2681SubstrateBy similarity
Binding sitei314 – 3141SubstrateBy similarity
Binding sitei321 – 3211SubstrateBy similarity
Binding sitei347 – 3471SubstrateBy similarity
Binding sitei399 – 3991SubstrateBy similarity
Binding sitei417 – 4171Substrate; via amide nitrogenBy similarity

GO - Molecular functioni

  1. glutaminase activity Source: UniProtKB-EC

GO - Biological processi

  1. glutamine metabolic process Source: InterPro
  2. reactive oxygen species metabolic process Source: MGI
  3. regulation of apoptotic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

ReactomeiREACT_229514. Glutamate Neurotransmitter Release Cycle.
REACT_239550. Amino acid synthesis and interconversion (transamination).

Names & Taxonomyi

Protein namesi
Recommended name:
Glutaminase liver isoform, mitochondrial (EC:3.5.1.2)
Short name:
GLS
Alternative name(s):
L-glutaminase
L-glutamine amidohydrolase
Gene namesi
Name:Gls2
Synonyms:Kiaa4146
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 10

Organism-specific databases

MGIiMGI:2143539. Gls2.

Subcellular locationi

Mitochondrion By similarity

GO - Cellular componenti

  1. mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 1414MitochondrionSequence AnalysisAdd
BLAST
Chaini15 – 602588Glutaminase liver isoform, mitochondrialPRO_0000011626Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei97 – 971N6-succinyllysineBy similarity
Modified residuei244 – 2441N6-acetyllysineBy similarity
Modified residuei253 – 2531N6-succinyllysine1 Publication
Modified residuei279 – 2791N6-acetyllysine1 Publication
Modified residuei284 – 2841N6-acetyllysine1 Publication
Modified residuei329 – 3291N6-acetyllysine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ571F8.
PaxDbiQ571F8.
PRIDEiQ571F8.

PTM databases

PhosphoSiteiQ571F8.

Expressioni

Gene expression databases

BgeeiQ571F8.
CleanExiMM_GLS2.
ExpressionAtlasiQ571F8. baseline and differential.
GenevestigatoriQ571F8.

Interactioni

Subunit structurei

Interacts with the PDZ domain of the syntrophin SNTA1. Interacts with the PDZ domain of TAX1BP3 (By similarity).By similarity

Protein-protein interaction databases

IntActiQ571F8. 1 interaction.
STRINGi10090.ENSMUSP00000047376.

Structurei

3D structure databases

ProteinModelPortaliQ571F8.
SMRiQ571F8. Positions 70-479, 490-572.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati518 – 55134ANK 1Add
BLAST
Repeati552 – 58534ANK 2Add
BLAST

Sequence similaritiesi

Belongs to the glutaminase family.Curated
Contains 2 ANK repeats.Curated

Keywords - Domaini

ANK repeat, Repeat, Transit peptide

Phylogenomic databases

eggNOGiCOG2066.
GeneTreeiENSGT00390000010463.
HOGENOMiHOG000216891.
HOVERGENiHBG005856.
InParanoidiQ571F8.
KOiK01425.
OMAiCVKPLTY.
OrthoDBiEOG7S4X5F.
PhylomeDBiQ571F8.
TreeFamiTF313359.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
3.40.710.10. 1 hit.
HAMAPiMF_00313. Glutaminase.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR012338. Beta-lactam/transpept-like.
IPR015868. Glutaminase.
[Graphical view]
PANTHERiPTHR12544. PTHR12544. 1 hit.
PfamiPF12796. Ank_2. 1 hit.
PF04960. Glutaminase. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 2 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF56601. SSF56601. 1 hit.
TIGRFAMsiTIGR03814. Gln_ase. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q571F8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRSMRALQNA LSRAGSHGRR GGWGHPSRGP LLGRGVRYYL GEAAAQGRGT
60 70 80 90 100
PHSHQPQHSD HDASHSGMLP RLGDLLFYTI AEGQERIPIH KFTTALKATG
110 120 130 140 150
LQTSDPRLQD CMSKMQRMVQ ESSSGGLLDR ELFQKCVSSN IVLLTQAFRK
160 170 180 190 200
KFVIPDFEEF TGHVDRIFED AKEPTGGKVA AYIPHLAKSN PDLWGVSLCT
210 220 230 240 250
VDGQRHSVGH TKIPFCLQSC VKPLTYAISV STLGTDYVHK FVGKEPSGLR
260 270 280 290 300
YNKLSLNEEG IPHNPMVNAG AIVVSSLIKM DCNKAEKFDF VLQYLNKMAG
310 320 330 340 350
NEFMGFSNAT FQSEKETGDR NYAIGYYLKE KKCFPKGVDM MAALDLYFQL
360 370 380 390 400
CSVEVTCESG SVMAATLANG GICPITGESV LSAEAVRNTL SLMHSCGMYD
410 420 430 440 450
FSGQFAFHVG LPAKSAVSGA ILLVVPNVMG MMCLSPPLDK LGNSQRGINF
460 470 480 490 500
CQKLVSLFNF HNYDNLRHCA RKLDPRREGG EVRNKTVVNL LFAAYSGDVS
510 520 530 540 550
ALRRFALSAM DMEQKDYDSR TALHVAAAEG HIEVVKFLIE ACKVNPFVKD
560 570 580 590 600
RWGNIPLDDA VQFNHLEVVK LLQDYHDSYL LSETQAEAAA ETLSKENLES

MV
Length:602
Mass (Da):66,366
Last modified:August 30, 2005 - v2
Checksum:iBA07D22ADB082F76
GO

Sequence cautioni

The sequence BAD90156.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti312 – 3121Q → K in BAE38445 (PubMed:16141072).Curated
Sequence conflicti584 – 5841T → S in AAI41116 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK220231 mRNA. Translation: BAD90156.1. Different initiation.
AK165896 mRNA. Translation: BAE38445.1.
BC141115 mRNA. Translation: AAI41116.1.
CCDSiCCDS24263.1.
RefSeqiNP_001028436.2. NM_001033264.3.
UniGeneiMm.30102.
Mm.447148.

Genome annotation databases

EnsembliENSMUST00000044776; ENSMUSP00000047376; ENSMUSG00000044005.
GeneIDi216456.
KEGGimmu:216456.
UCSCiuc007hlo.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK220231 mRNA. Translation: BAD90156.1. Different initiation.
AK165896 mRNA. Translation: BAE38445.1.
BC141115 mRNA. Translation: AAI41116.1.
CCDSiCCDS24263.1.
RefSeqiNP_001028436.2. NM_001033264.3.
UniGeneiMm.30102.
Mm.447148.

3D structure databases

ProteinModelPortaliQ571F8.
SMRiQ571F8. Positions 70-479, 490-572.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ571F8. 1 interaction.
STRINGi10090.ENSMUSP00000047376.

Chemistry

BindingDBiQ571F8.
ChEMBLiCHEMBL2176860.

PTM databases

PhosphoSiteiQ571F8.

Proteomic databases

MaxQBiQ571F8.
PaxDbiQ571F8.
PRIDEiQ571F8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000044776; ENSMUSP00000047376; ENSMUSG00000044005.
GeneIDi216456.
KEGGimmu:216456.
UCSCiuc007hlo.2. mouse.

Organism-specific databases

CTDi27165.
MGIiMGI:2143539. Gls2.
RougeiSearch...

Phylogenomic databases

eggNOGiCOG2066.
GeneTreeiENSGT00390000010463.
HOGENOMiHOG000216891.
HOVERGENiHBG005856.
InParanoidiQ571F8.
KOiK01425.
OMAiCVKPLTY.
OrthoDBiEOG7S4X5F.
PhylomeDBiQ571F8.
TreeFamiTF313359.

Enzyme and pathway databases

ReactomeiREACT_229514. Glutamate Neurotransmitter Release Cycle.
REACT_239550. Amino acid synthesis and interconversion (transamination).

Miscellaneous databases

NextBioi375178.
PROiQ571F8.
SOURCEiSearch...

Gene expression databases

BgeeiQ571F8.
CleanExiMM_GLS2.
ExpressionAtlasiQ571F8. baseline and differential.
GenevestigatoriQ571F8.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
3.40.710.10. 1 hit.
HAMAPiMF_00313. Glutaminase.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR012338. Beta-lactam/transpept-like.
IPR015868. Glutaminase.
[Graphical view]
PANTHERiPTHR12544. PTHR12544. 1 hit.
PfamiPF12796. Ank_2. 1 hit.
PF04960. Glutaminase. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 2 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF56601. SSF56601. 1 hit.
TIGRFAMsiTIGR03814. Gln_ase. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O., Koga H.
    Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Spleen.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.
  4. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-253, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  5. "Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
    Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
    Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-279; LYS-284 AND LYS-329, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiGLSL_MOUSE
AccessioniPrimary (citable) accession number: Q571F8
Secondary accession number(s): B2RUF2, Q3TMJ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: August 30, 2005
Last modified: March 4, 2015
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.