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Q571F8 (GLSL_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutaminase liver isoform, mitochondrial

Short name=GLS
EC=3.5.1.2
Alternative name(s):
L-glutaminase
L-glutamine amidohydrolase
Gene names
Name:Gls2
Synonyms:Kiaa4146
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length602 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH and glutathione production By similarity. HAMAP-Rule MF_00313

Catalytic activity

L-glutamine + H2O = L-glutamate + NH3. HAMAP-Rule MF_00313

Subunit structure

Interacts with the PDZ domain of the syntrophin SNTA1. Interacts with the PDZ domain of TAX1BP3 By similarity.

Subcellular location

Mitochondrion By similarity HAMAP-Rule MF_00313.

Sequence similarities

Belongs to the glutaminase family.

Contains 2 ANK repeats.

Sequence caution

The sequence BAD90156.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 1414Mitochondrion Potential
Chain15 – 602588Glutaminase liver isoform, mitochondrial HAMAP-Rule MF_00313
PRO_0000011626

Regions

Repeat518 – 55134ANK 1 HAMAP-Rule MF_00313
Repeat552 – 58534ANK 2 HAMAP-Rule MF_00313

Sites

Binding site2191Substrate By similarity
Binding site2681Substrate By similarity
Binding site3141Substrate By similarity
Binding site3211Substrate By similarity
Binding site3471Substrate By similarity
Binding site3991Substrate By similarity
Binding site4171Substrate; via amide nitrogen By similarity

Amino acid modifications

Modified residue2531N6-succinyllysine Ref.4
Modified residue2791N6-acetyllysine Ref.5
Modified residue2841N6-acetyllysine Ref.5
Modified residue3291N6-acetyllysine Ref.5

Experimental info

Sequence conflict3121Q → K in BAE38445. Ref.2
Sequence conflict5841T → S in AAI41116. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q571F8 [UniParc].

Last modified August 30, 2005. Version 2.
Checksum: BA07D22ADB082F76

FASTA60266,366
        10         20         30         40         50         60 
MRSMRALQNA LSRAGSHGRR GGWGHPSRGP LLGRGVRYYL GEAAAQGRGT PHSHQPQHSD 

        70         80         90        100        110        120 
HDASHSGMLP RLGDLLFYTI AEGQERIPIH KFTTALKATG LQTSDPRLQD CMSKMQRMVQ 

       130        140        150        160        170        180 
ESSSGGLLDR ELFQKCVSSN IVLLTQAFRK KFVIPDFEEF TGHVDRIFED AKEPTGGKVA 

       190        200        210        220        230        240 
AYIPHLAKSN PDLWGVSLCT VDGQRHSVGH TKIPFCLQSC VKPLTYAISV STLGTDYVHK 

       250        260        270        280        290        300 
FVGKEPSGLR YNKLSLNEEG IPHNPMVNAG AIVVSSLIKM DCNKAEKFDF VLQYLNKMAG 

       310        320        330        340        350        360 
NEFMGFSNAT FQSEKETGDR NYAIGYYLKE KKCFPKGVDM MAALDLYFQL CSVEVTCESG 

       370        380        390        400        410        420 
SVMAATLANG GICPITGESV LSAEAVRNTL SLMHSCGMYD FSGQFAFHVG LPAKSAVSGA 

       430        440        450        460        470        480 
ILLVVPNVMG MMCLSPPLDK LGNSQRGINF CQKLVSLFNF HNYDNLRHCA RKLDPRREGG 

       490        500        510        520        530        540 
EVRNKTVVNL LFAAYSGDVS ALRRFALSAM DMEQKDYDSR TALHVAAAEG HIEVVKFLIE 

       550        560        570        580        590        600 
ACKVNPFVKD RWGNIPLDDA VQFNHLEVVK LLQDYHDSYL LSETQAEAAA ETLSKENLES 


MV 

« Hide

References

[1]"Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O., Koga H.
Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Spleen.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Lung.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Lung.
[4]"SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-253, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[5]"Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-279; LYS-284 AND LYS-329, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK220231 mRNA. Translation: BAD90156.1. Different initiation.
AK165896 mRNA. Translation: BAE38445.1.
BC141115 mRNA. Translation: AAI41116.1.
RefSeqNP_001028436.2. NM_001033264.3.
UniGeneMm.30102.
Mm.447148.

3D structure databases

ProteinModelPortalQ571F8.
SMRQ571F8. Positions 70-582.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ571F8. 1 interaction.
STRING10090.ENSMUSP00000047376.

Chemistry

ChEMBLCHEMBL2176860.

PTM databases

PhosphoSiteQ571F8.

Proteomic databases

PaxDbQ571F8.
PRIDEQ571F8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000044776; ENSMUSP00000047376; ENSMUSG00000044005.
GeneID216456.
KEGGmmu:216456.
UCSCuc007hlo.2. mouse.

Organism-specific databases

CTD27165.
MGIMGI:2143539. Gls2.
RougeSearch...

Phylogenomic databases

eggNOGCOG2066.
GeneTreeENSGT00390000010463.
HOGENOMHOG000216891.
HOVERGENHBG005856.
InParanoidQ3TMJ6.
KOK01425.
OMAEGGEVRN.
OrthoDBEOG7S4X5F.
PhylomeDBQ571F8.
TreeFamTF313359.

Gene expression databases

ArrayExpressQ571F8.
BgeeQ571F8.
CleanExMM_GLS2.
GenevestigatorQ571F8.

Family and domain databases

Gene3D1.25.40.20. 1 hit.
3.40.710.10. 1 hit.
HAMAPMF_00313. Glutaminase.
InterProIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR012338. Beta-lactam/transpept-like.
IPR015868. Glutaminase.
[Graphical view]
PANTHERPTHR12544. PTHR12544. 1 hit.
PfamPF12796. Ank_2. 1 hit.
PF04960. Glutaminase. 1 hit.
[Graphical view]
SMARTSM00248. ANK. 2 hits.
[Graphical view]
SUPFAMSSF48403. SSF48403. 1 hit.
SSF56601. SSF56601. 1 hit.
TIGRFAMsTIGR03814. Gln_ase. 1 hit.
PROSITEPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio375178.
PROQ571F8.
SOURCESearch...

Entry information

Entry nameGLSL_MOUSE
AccessionPrimary (citable) accession number: Q571F8
Secondary accession number(s): B2RUF2, Q3TMJ6
Entry history
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: August 30, 2005
Last modified: April 16, 2014
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot