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Protein

N-acetylgalactosamine-6-sulfatase

Gene

Galns

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of the 6-sulfate groups of the N-acetyl-D-galactosamine 6-sulfate units of chondroitin sulfate and of the D-galactose 6-sulfate units of keratan sulfate.

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi36CalciumBy similarity1
Metal bindingi37CalciumBy similarity1
Metal bindingi76Calcium; via 3-oxoalanineBy similarity1
Metal bindingi286CalciumBy similarity1
Metal bindingi287CalciumBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
LigandCalcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.1.6.4. 3474.
ReactomeiR-MMU-2022857. Keratan sulfate degradation.
R-MMU-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetylgalactosamine-6-sulfatase (EC:3.1.6.4)
Alternative name(s):
Chondroitinsulfatase
Short name:
Chondroitinase
Galactose-6-sulfate sulfatase
N-acetylgalactosamine-6-sulfate sulfatase
Short name:
GalNAc6S sulfatase
Gene namesi
Name:Galns
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1355303. Galns.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Lysosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23By similarityAdd BLAST23
ChainiPRO_000027314824 – 520N-acetylgalactosamine-6-sulfataseAdd BLAST497

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei763-oxoalanine (Cys)By similarity1
Glycosylationi201N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi306 ↔ 417By similarity
Glycosylationi421N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi487 ↔ 516By similarity
Disulfide bondi499 ↔ 505By similarity

Post-translational modificationi

The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiQ571E4.
MaxQBiQ571E4.
PaxDbiQ571E4.
PeptideAtlasiQ571E4.
PRIDEiQ571E4.

PTM databases

PhosphoSitePlusiQ571E4.

Expressioni

Tissue specificityi

Widely expressed. Higher expression in liver and kidney.1 Publication

Gene expression databases

BgeeiENSMUSG00000015027.
CleanExiMM_GALNS.
ExpressionAtlasiQ571E4. baseline and differential.
GenevisibleiQ571E4. MM.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000015171.

Structurei

3D structure databases

ProteinModelPortaliQ571E4.
SMRiQ571E4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni24 – 377Catalytic domainBy similarityAdd BLAST354

Sequence similaritiesi

Belongs to the sulfatase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3867. Eukaryota.
COG3119. LUCA.
GeneTreeiENSGT00760000119062.
HOGENOMiHOG000135352.
HOVERGENiHBG004283.
InParanoidiQ571E4.
KOiK01132.
OMAiWEEFRQG.
OrthoDBiEOG091G07AL.
PhylomeDBiQ571E4.
TreeFamiTF314186.

Family and domain databases

Gene3Di3.40.720.10. 2 hits.
InterProiView protein in InterPro
IPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR024607. Sulfatase_CS.
IPR000917. Sulfatase_N.
PfamiView protein in Pfam
PF00884. Sulfatase. 1 hit.
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiView protein in PROSITE
PS00523. SULFATASE_1. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q571E4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAACTAAQQL LLVLSALGLL AAGAPQPPNI VLLLMDDMGW GDLGVNGEPS
60 70 80 90 100
RETPNLDRMA AEGMLFPSFY SANPLCSPSR AALLTGRLPI RNGFYTTNAH
110 120 130 140 150
ARNAYTPQEI MGGIPNSEHL LPELLKKAGY TNKIVGKWHL GHRPQFHPLK
160 170 180 190 200
HGFDEWFGSP NCHFGPYDNK AKPNIPVYRD WEMVGRFYEE FPINRKTGEA
210 220 230 240 250
NLTQLYTQEA LDFIQTQHAR QSPFFLYWAI DATHAPVYAS RQFLGTSLRG
260 270 280 290 300
RYGDAVREID DSVGKILSLL QNLGISKNTF VFFTSDNGAA LISAPNEGGS
310 320 330 340 350
NGPFLCGKQT TFEGGMREPA IAWWPGHIAA GQVSHQLGSI MDLFTTSLSL
360 370 380 390 400
AGLKPPSDRV IDGLDLLPTM LKGQMMDRPI FYYRGNTLMA VTLGQYKAHL
410 420 430 440 450
WTWTNSWEEF TQGTDFCPGQ NVSGVTTHTQ EEHTELPLIF HLGRDPGERF
460 470 480 490 500
PLSFHSDEYQ DALSRTTQVV QEHQKSLVPG QPQLNVCNQA VMNWAPPGCE
510 520
KLGKCLTPPE SVPEKCFWAH
Length:520
Mass (Da):57,673
Last modified:January 23, 2007 - v2
Checksum:i63428DFA8843A21D
GO

Sequence cautioni

The sequence BAD90170 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti22A → V in AAH04002 (PubMed:15489334).Curated1
Sequence conflicti154D → N in AAF63858 (PubMed:10699374).Curated1
Sequence conflicti154D → N in AAF63155 (PubMed:10699374).Curated1
Sequence conflicti207T → L in AAH04002 (PubMed:15489334).Curated1
Sequence conflicti215Q → R in AAH04002 (PubMed:15489334).Curated1
Sequence conflicti222S → G in AAH04002 (PubMed:15489334).Curated1
Sequence conflicti268S → N in AAH04002 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF111346 mRNA. Translation: AAF63155.1.
AF112242
, AF112230, AF112231, AF112233, AF112232, AF112234, AF112235, AF112236, AF112237, AF112238, AF112239, AF112240, AF112241 Genomic DNA. Translation: AAF63858.1.
AK220245 mRNA. Translation: BAD90170.1. Different initiation.
AK159592 mRNA. Translation: BAE35212.1.
BC004002 mRNA. Translation: AAH04002.1.
CCDSiCCDS40504.1.
RefSeqiNP_001180574.1. NM_001193645.1.
NP_057931.3. NM_016722.4.
UniGeneiMm.1786.
Mm.34702.

Genome annotation databases

EnsembliENSMUST00000015171; ENSMUSP00000015171; ENSMUSG00000015027.
GeneIDi50917.
KEGGimmu:50917.
UCSCiuc012gmh.1. mouse.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiGALNS_MOUSE
AccessioniPrimary (citable) accession number: Q571E4
Secondary accession number(s): Q3TWQ4, Q99KU8, Q9JHK9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: January 23, 2007
Last modified: June 7, 2017
This is version 94 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families