Reviewed, UniProtKB/Swiss-Prot Q57179 (ILVC_CORGL)

Names and origin

Protein names

Recommended name:
    Ketol-acid reductoisomerase
    EC=1.1.1.86
Alternative name(s):
    Acetohydroxy-acid isomeroreductase
    Alpha-keto-beta-hydroxylacil reductoisomerase

Gene names

Name:	ilvC
Ordered Locus Names:	Cgl1273, cg1437

Organism

Corynebacterium glutamicum (Brevibacterium flavum) [Complete proteome] [HAMAP]

Taxonomic identifier

1718 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	338 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. HAMAP MF_00435 (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH. HAMAP MF_00435
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. HAMAP MF_00435 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. HAMAP MF_00435
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 338	338	Ketol-acid reductoisomerase HAMAP MF_00435		PRO_0000151306
Sites
	Active site	109	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q57179-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 9BA1697FA7D2A082	FASTA	338	36,159
	10 20 30 40 50 60 MAIELLYDAD ADLSLIQGRK VAIVGYGSQG HAHSQNLRDS GVEVVIGLRE GSKSAEKAKE 70 80 90 100 110 120 AGFEVKTTAE AAAWADVIML LAPDTSQAEI FTNDIEPNLN AGDALLFGHG LNIHFDLIKP 130 140 150 160 170 180 ADDIIVGMVA PKGPGHLVRR QFVDGKGVPC LIAVDQDPTG TAQALTLSYA AAIGGARAGV 190 200 210 220 230 240 IPTTFEAETV TDLFGEQAVL CGGTEELVKV GFEVLTEAGY EPEMAYFEVL HELKLIVDLM 250 260 270 280 290 300 FEGGISNMNY SVSDTAEFGG YLSGPRVIDA DTKSRMKDIL TDIQDGTFTK RLIANVENGN 310 320 330 TELEGLRASY NNHPIEETGA KLRDLMSWVK VDARAETA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isoleucine synthesis in Corynebacterium glutamicum: molecular analysis of the ilvB-ilvN-ilvC operon." Keilhauer C., Eggeling L., Sahm H. J. Bacteriol. 175:5595-5603(1993) [PubMed: 8366043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[2]	"Identification and sequence determination of the acetohydroxy acid isomeroreductase gene from Brevibacterium flavum MJ233." Inui M., Vertes A.A., Kobayashi M., Kurusu Y., Yukawa H. DNA Seq. 4:95-103(1993) [PubMed: 8173081] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: MJ233.
[3]	"Complete genomic sequence of Corynebacterium glutamicum ATCC 13032." Nakagawa S. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[4]	"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins." Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A. J. Biotechnol. 104:5-25(2003) [PubMed: 12948626] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L09232 Genomic DNA. Translation: AAA62431.1. D14551 Genomic DNA. Translation: BAA03414.1. BA000036 Genomic DNA. Translation: BAB98666.1. BX927151 Genomic DNA. Translation: CAF19976.1.
PIR	C48648.
RefSeq	NP_600495.1. YP_225562.1.
3D structure databases
HSSP	HSSP built from PDB template 1NP3 based on UniProtKB Q9HVA2.
ModBase	Search...
2-D gel databases
World-2DPAGE	0001:Q57179.
Proteomic databases
PRIDE	Q57179.
Genome annotation databases
GeneID	1019254. 3343961.
GenomeReviews	Gene locus Cgl1273 in contig BA000036_GR. Gene locus cg1437 in contig BX927147_GR.
KEGG	cgb:cg1437. cgl:NCgl1224.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q57179.
OMA	Q57179. AHGFNIR.
Enzyme and pathway databases
BioCyc	CGLU196627-1:CG1437-MON.
BRENDA	1.1.1.86. 812.
Family and domain databases
HAMAP	MF_00435. [Tree]
InterPro	IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013116. IlvN. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
TIGRFAMs	TIGR00465. ilvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC_CORGL

Accession

Primary (citable) accession number: Q57179

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)