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Protein

Ketol-acid reductoisomerase (NADP(+))

Gene

ilvC

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate.UniRule annotation

Catalytic activityi

(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH.UniRule annotation
(2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 2 magnesium ions per subunit.UniRule annotation

Pathwayi: L-isoleucine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-isoleucine from 2-oxobutanoate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Acetolactate synthase large subunit (ilvB)
  2. Ketol-acid reductoisomerase (NADP(+)) (ilvC)
  3. Dihydroxy-acid dehydratase (ilvD)
  4. no protein annotated in this organism
This subpathway is part of the pathway L-isoleucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-isoleucine from 2-oxobutanoate, the pathway L-isoleucine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: L-valine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-valine from pyruvate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Acetolactate synthase large subunit (ilvB)
  2. Ketol-acid reductoisomerase (NADP(+)) (ilvC)
  3. Dihydroxy-acid dehydratase (ilvD)
  4. no protein annotated in this organism
This subpathway is part of the pathway L-valine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-valine from pyruvate, the pathway L-valine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei49NADPUniRule annotation1
Binding sitei52NADPUniRule annotation1
Binding sitei54NADPUniRule annotation1
Active sitei109UniRule annotation1
Binding sitei135NADP; via amide nitrogenUniRule annotation1
Metal bindingi192Magnesium 1UniRule annotation1
Metal bindingi192Magnesium 2UniRule annotation1
Metal bindingi196Magnesium 1UniRule annotation1
Metal bindingi228Magnesium 2UniRule annotation1
Metal bindingi232Magnesium 2UniRule annotation1
Binding sitei253SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi26 – 29NADPUniRule annotation4
Nucleotide bindingi84 – 87NADPUniRule annotation4

GO - Molecular functioni

  • 2-dehydropantoate 2-reductase activity Source: CACAO
  • ketol-acid reductoisomerase activity Source: UniProtKB-EC
  • metal ion binding Source: UniProtKB-KW
  • NADP binding Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processAmino-acid biosynthesis, Branched-chain amino acid biosynthesis
LigandMagnesium, Metal-binding, NADP

Enzyme and pathway databases

BioCyciCORYNE:G18NG-10846-MONOMER
UniPathwayiUPA00047; UER00056
UPA00049; UER00060

Names & Taxonomyi

Protein namesi
Recommended name:
Ketol-acid reductoisomerase (NADP(+))UniRule annotation (EC:1.1.1.86UniRule annotation)
Short name:
KARIUniRule annotation
Alternative name(s):
Acetohydroxy-acid isomeroreductaseUniRule annotation
Short name:
AHIRUniRule annotation
Alpha-keto-beta-hydroxylacyl reductoisomeraseUniRule annotation
Ketol-acid reductoisomerase type 1UniRule annotation
Ketol-acid reductoisomerase type IUniRule annotation
Gene namesi
Name:ilvCUniRule annotation
Ordered Locus Names:Cgl1273, cg1437
OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Taxonomic identifieri196627 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
Proteomesi
  • UP000000582 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001513061 – 338Ketol-acid reductoisomerase (NADP(+))Add BLAST338

2D gel databases

World-2DPAGE0001:Q57179

Interactioni

Protein-protein interaction databases

STRINGi196627.cg1437

Structurei

3D structure databases

ProteinModelPortaliQ57179
SMRiQ57179
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 183KARI N-terminal RossmannPROSITE-ProRule annotationAdd BLAST181
Domaini184 – 329KARI C-terminal knottedPROSITE-ProRule annotationAdd BLAST146

Sequence similaritiesi

Belongs to the ketol-acid reductoisomerase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0059 LUCA
HOGENOMiHOG000016230
KOiK00053
OMAiRAMFSWL

Family and domain databases

HAMAPiMF_00435 IlvC, 1 hit
InterProiView protein in InterPro
IPR008927 6-PGluconate_DH-like_C_sf
IPR013023 KARI
IPR000506 KARI_C
IPR013116 KARI_N
IPR014359 KARI_prok
IPR036291 NAD(P)-bd_dom_sf
PANTHERiPTHR21371 PTHR21371, 1 hit
PfamiView protein in Pfam
PF01450 IlvC, 1 hit
PF07991 IlvN, 1 hit
PIRSFiPIRSF000116 IlvC_gammaproteo, 1 hit
SUPFAMiSSF48179 SSF48179, 1 hit
SSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR00465 ilvC, 1 hit
PROSITEiView protein in PROSITE
PS51851 KARI_C, 1 hit
PS51850 KARI_N, 1 hit

Sequencei

Sequence statusi: Complete.

Q57179-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIELLYDAD ADLSLIQGRK VAIVGYGSQG HAHSQNLRDS GVEVVIGLRE
60 70 80 90 100
GSKSAEKAKE AGFEVKTTAE AAAWADVIML LAPDTSQAEI FTNDIEPNLN
110 120 130 140 150
AGDALLFGHG LNIHFDLIKP ADDIIVGMVA PKGPGHLVRR QFVDGKGVPC
160 170 180 190 200
LIAVDQDPTG TAQALTLSYA AAIGGARAGV IPTTFEAETV TDLFGEQAVL
210 220 230 240 250
CGGTEELVKV GFEVLTEAGY EPEMAYFEVL HELKLIVDLM FEGGISNMNY
260 270 280 290 300
SVSDTAEFGG YLSGPRVIDA DTKSRMKDIL TDIQDGTFTK RLIANVENGN
310 320 330
TELEGLRASY NNHPIEETGA KLRDLMSWVK VDARAETA
Length:338
Mass (Da):36,159
Last modified:November 1, 1996 - v1
Checksum:i9BA1697FA7D2A082
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L09232 Genomic DNA Translation: AAA62431.1
D14551 Genomic DNA Translation: BAA03414.1
BA000036 Genomic DNA Translation: BAB98666.1
BX927151 Genomic DNA Translation: CAF19976.1
PIRiC48648
RefSeqiNP_600495.1, NC_003450.3
WP_003854117.1, NC_006958.1

Genome annotation databases

EnsemblBacteriaiBAB98666; BAB98666; BAB98666
CAF19976; CAF19976; cg1437
GeneIDi1019254
KEGGicgb:cg1437
cgl:NCgl1224
PATRICifig|196627.13.peg.1250

Similar proteinsi

Entry informationi

Entry nameiILVC_CORGL
AccessioniPrimary (citable) accession number: Q57179
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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