Q57168 (T1MH_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative type I restriction enzyme HindVIIP M protein
Short name=M.HindVIIP
EC=2.1.1.72

Gene names

Ordered Locus Names:

HI_1287

Organism

Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) [Reference proteome] [HAMAP]

Taxonomic identifier

71421 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus › Haemophilus influenzae

Protein attributes

Sequence length	443 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Methylation of specific adenine residues; required for both restriction and modification activities By similarity.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Subunit structure	The type I restriction/modification system is composed of three polypeptides R, M and S.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA methylation on adenine Inferred from electronic annotation. Source: GOC DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro N-methyltransferase activity Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 443	443	Putative type I restriction enzyme HindVIIP M protein		PRO_0000088027
Regions
	Region	117 – 122	6	S-adenosyl-L-methionine binding By similarity
	Region	146 – 148	3	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	173	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q57168 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 70573FBFFA78594C	FASTA	443	49,732
	10 20 30 40 50 60 MPASARWQAL QEVSILNTGA ELPWGGKFSG VAKLIDDAFD AIEKDNEKLK GVLQRISGYA 70 80 90 100 110 120 VNEDTLRGLI ILFSDTHFTR PTYNGEPVHL GAKDILGHVY EYFLSRFAQA EGKRSGQYFT 130 140 150 160 170 180 PKSIVSLIVE MLEPYSGRVY DPAMGSGGFF VQTERFITAH QGNINNVSIY GQEFNPTTWK 190 200 210 220 230 240 LAAMNMAIRG IDYDFGKYNA DSFTQPQHID KKMDFIMANP HFNDKEWWNE SLADDPRWAY 250 260 270 280 290 300 GTPPKGNANF AWLQHMIYHL SPNGKIALLL ANGSMSSQTN NEGEIRKAII NADLVECMVA 310 320 330 340 350 360 LPGQLFTNTK IPACIWFLNR NKKRKGEVLF IDARQIGYMK DRVLRDFTAD DIAKIADTLH 370 380 390 400 410 420 AWQTSDGYED QAAFCKSATL EEIKNNDFVL TPGRYVGTAE QEDDGVPFAE KMQNLTALLK 430 440 EQFAKSAELE AEIKKNLGGL GYE

References

[1]

"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L42023 Genomic DNA. Translation: AAC22936.1.
PIR	G64114.
RefSeq	NP_439439.2. NC_000907.1.
3D structure databases
ProteinModelPortal	Q57168.
ModBase	Search...
Protein-protein interaction databases
STRING	71421.HI1287.
Protein family/group databases
REBASE	3426. M.HindI.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC22936; AAC22936; HI_1287.
GeneID	950217.
KEGG	hin:HI1287.
PATRIC	20191257. VBIHaeInf48452_1339.
Phylogenomic databases
eggNOG	COG0286.
KO	K03427.
OMA	GYMKDRV.
ProtClustDB	CLSK824767.
Family and domain databases
InterPro	IPR003356. DNA_methylase_A-5. IPR002296. N12N6_MeTrfase. [Graphical view]
Pfam	PF02384. N6_Mtase. 1 hit. [Graphical view]
PRINTS	PR00507. N12N6MTFRASE.
PROSITE	PS00092. N6_MTASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T1MH_HAEIN

Accession

Primary (citable) accession number: Q57168
Secondary accession number(s): O05053

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program