Reviewed, UniProtKB/Swiss-Prot Q57163 (MIAB_HAEIN)

Names and origin

Protein names

Recommended name:
    (Dimethylallyl)adenosine tRNA methylthiotransferase miaB
    EC=2.-.-.-
Alternative name(s):
    tRNA-i(6)A37 methylthiotransferase

Gene names

Name:	miaB
Ordered Locus Names:	HI0019

Organism

Haemophilus influenzae [Complete proteome] [HAMAP]

Taxonomic identifier

727 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	474 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that read codons beginning with uridine By similarity.
Cofactor	Binds 2 4Fe-4S clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the methylthiotransferase family. MiaB subfamily. Contains 1 MTTase N-terminal domain. Contains 1 TRAM domain.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA modification Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW transferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 474	474	(Dimethylallyl)adenosine tRNA methylthiotransferase miaB HAMAP MF_01864		PRO_0000141737
Regions
	Domain	3 – 120	118	MTTase N-terminal
	Domain	378 – 441	64	TRAM
Sites
	Metal binding	12	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	49	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	83	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	157	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
	Metal binding	161	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
	Metal binding	164	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q57163-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: A6ECDFBBAE16923D	FASTA	474	53,640
	10 20 30 40 50 60 MTQKLHIKTW GCQMNEYDSS KMADLLLSTH GLELTEAPEE ADVLLLNTCS IREKAQEKVF 70 80 90 100 110 120 HQLGRWKELK KNNPNLVIGV GGCVASQEGE HIRHRAPYVD IIFGPQTLHR LPEMINQIRG 130 140 150 160 170 180 GKSSVVDVSF PEIEKFDRLP EPRAEGPTAF VSIMEGCNKY CTFCVVPYTR GEEVSRPVDD 190 200 210 220 230 240 VLFEIAQLAE QGVREVNLLG QNVNAYRGPT HDGQICSFAE LLRLVASIDG IDRLRFTTSH 250 260 270 280 290 300 PIEFTDDIID VYRDTPELVS FLHLPVQAGS DRVLTMMKRA HTALEYKSII RKLRAVRPDI 310 320 330 340 350 360 QISSDFIVGF PGETAEDFEQ TMNLIAQVNF DMSFSFVYSA RPGTPAADMP DDVTEDEKKQ 370 380 390 400 410 420 RLYVLQERIN QQAAQFSRRM LGTEQRVLVE GPSKKDIMEL TGRTETNRIV NFQGSPEMIG 430 440 450 460 470 KFVDVKITDV YTNSLRGEVV RTEDEMGLRI AQSPQEVMNR TRKEDELGVG RYHG

References

[1]

"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed: 7542800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L42023 Genomic DNA. Translation: AAC21697.1.
PIR	D64140.
RefSeq	NP_438192.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	950916.
GenomeReviews	Gene locus HI0019 in contig L42023_GR.
KEGG	hin:HI0019.
TIGR	HI0019.
Phylogenomic databases
HOGENOM	Q57163.
OMA	AANLHDD
Enzyme and pathway databases
BioCyc	HINF71421:HI_0019-MON.
Family and domain databases
HAMAP	MF_01864. [Tree]
InterPro	IPR006638. Elp3/MiaB/NifB. IPR005839. Methylthiotransferase. IPR020612. Methylthiotransferase_CS. IPR013848. Methylthiotransferase_N. IPR006463. MiaB_methiolase. IPR007197. Radical_SAM. IPR002792. TRAM. [Graphical view]
PANTHER	PTHR11918. UPF0004. 1 hit.
Pfam	PF04055. Radical_SAM. 1 hit. PF01938. TRAM. 1 hit. PF00919. UPF0004. 1 hit. [Graphical view]
SMART	SM00729. Elp3. 1 hit. [Graphical view]
TIGRFAMs	TIGR01574. miaB-methiolase. 1 hit. TIGR00089. UPF0004. 1 hit.
PROSITE	PS51449. MTTASE_N. 1 hit. PS01278. MTTASE_RADICAL. 1 hit. PS50926. TRAM. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MIAB_HAEIN

Accession

Primary (citable) accession number: Q57163

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	November 24, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)