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Q57163 (MIAB_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB
EC=2.-.-.-
Alternative name(s):
tRNA-i(6)A37 methylthiotransferase
Gene names
Name:miaB
Ordered Locus Names:HI_0019
OrganismHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifier71421 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length474 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that read codons beginning with uridine By similarity. HAMAP MF_01864

Cofactor

Binds 2 4Fe-4S clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Subunit structure

Monomer By similarity. HAMAP MF_01864

Subcellular location

Cytoplasm Potential HAMAP MF_01864.

Sequence similarities

Belongs to the methylthiotransferase family. MiaB subfamily.

Contains 1 MTTase N-terminal domain.

Contains 1 TRAM domain.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentCytoplasm
   Ligand4Fe-4S
Iron
Iron-sulfur
Metal-binding
S-adenosyl-L-methionine
   Molecular functionTransferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processtRNA modification

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular function4 iron, 4 sulfur cluster binding

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

transferase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 474474(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB HAMAP MF_01864
PRO_0000141737

Regions

Domain3 – 120118MTTase N-terminal
Domain378 – 44164TRAM

Sites

Metal binding121Iron-sulfur (4Fe-4S) By similarity
Metal binding491Iron-sulfur (4Fe-4S) By similarity
Metal binding831Iron-sulfur (4Fe-4S) By similarity
Metal binding1571Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal binding1611Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal binding1641Iron-sulfur (4Fe-4S-S-AdoMet) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q57163 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: A6ECDFBBAE16923D

FASTA47453,640
        10         20         30         40         50         60 
MTQKLHIKTW GCQMNEYDSS KMADLLLSTH GLELTEAPEE ADVLLLNTCS IREKAQEKVF 

        70         80         90        100        110        120 
HQLGRWKELK KNNPNLVIGV GGCVASQEGE HIRHRAPYVD IIFGPQTLHR LPEMINQIRG 

       130        140        150        160        170        180 
GKSSVVDVSF PEIEKFDRLP EPRAEGPTAF VSIMEGCNKY CTFCVVPYTR GEEVSRPVDD 

       190        200        210        220        230        240 
VLFEIAQLAE QGVREVNLLG QNVNAYRGPT HDGQICSFAE LLRLVASIDG IDRLRFTTSH 

       250        260        270        280        290        300 
PIEFTDDIID VYRDTPELVS FLHLPVQAGS DRVLTMMKRA HTALEYKSII RKLRAVRPDI 

       310        320        330        340        350        360 
QISSDFIVGF PGETAEDFEQ TMNLIAQVNF DMSFSFVYSA RPGTPAADMP DDVTEDEKKQ 

       370        380        390        400        410        420 
RLYVLQERIN QQAAQFSRRM LGTEQRVLVE GPSKKDIMEL TGRTETNRIV NFQGSPEMIG 

       430        440        450        460        470 
KFVDVKITDV YTNSLRGEVV RTEDEMGLRI AQSPQEVMNR TRKEDELGVG RYHG

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		L42023 Genomic DNA. Translation: AAC21697.1.
PIRD64140.
RefSeqNP_438192.1. NC_000907.1.

3D structure databases

ProteinModelPortalQ57163.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID950916.
GenomeReviewsGene locus HI_0019 in contig L42023_GR.
KEGGhin:HI0019.
PATRIC20188489. VBIHaeInf48452_0019.
TIGRHI_0019.

Phylogenomic databases

HOGENOMHBG457663.
OMAANLHDDT.
PhylomeDBQ57163.
ProtClustDBPRK14325.

Enzyme and pathway databases

BioCycHINF71421:HI_0019-MONOMER.

Family and domain databases

HAMAPMF_01864. tRNA_metthiotr_MiaB.
[Tree]
InterProIPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/B12-bd/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR006463. MiaB_methiolase.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM.
[Graphical view]
Gene3DG3DSA:3.80.30.20. G3DSA:3.80.30.20. 1 hit.
KOK06168.
PANTHERPTHR11918. UPF0004. 1 hit.
PfamPF04055. Radical_SAM. 1 hit.
PF01938. TRAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view]
SMARTSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR01574. MiaB-methiolase. 1 hit.
TIGR00089. TIGR00089. 1 hit.
PROSITEPS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMIAB_HAEIN
AccessionPrimary (citable) accession number: Q57163
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: January 25, 2012
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Haemophilus influenzae

Haemophilus influenzae (strain Rd): entries and gene names

SIMILARITY comments

Index of protein domains and families

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