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Protein

4-hydroxyphenylacetate 3-monooxygenase oxygenase component

Gene

hpaB

Organism
Escherichia coli
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Utilizes FADH2 supplied by HpaC or by another flavin reductase, to catalyze the hydroxylation of 4-hydroxyphenylacetic acid, leading to the production of 3,4-DHPA. Can also oxidize phenol to catechol, and hydroxylate other phenol derivatives.1 Publication

Miscellaneous

E.coli K12 lacks the oxygenase component HpaB.

Catalytic activityi

4-hydroxyphenylacetate + FADH2 + O2 = 3,4-dihydroxyphenylacetate + FAD + H2O.1 Publication

Pathwayi: 4-hydroxyphenylacetate degradation

This protein is involved in step 1 of the subpathway that synthesizes pyruvate and succinate semialdehyde from 4-hydroxyphenylacetate.
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. 4-hydroxyphenylacetate 3-monooxygenase reductase component (hpaC), 4-hydroxyphenylacetate 3-monooxygenase oxygenase component (hpaB)
  2. 3,4-dihydroxyphenylacetate 2,3-dioxygenase (hpcB)
  3. 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase (hpcC)
  4. 4-hydroxyphenylacetate degradation bifunctional isomerase/decarboxylase (hpaG), 5-carboxymethyl-2-hydroxymuconate Delta-isomerase (hpcD), Homoprotocatechuate catabolism bifunctional isomerase/decarboxylase (hpcE)
  5. 4-hydroxyphenylacetate degradation bifunctional isomerase/decarboxylase (hpaG), Homoprotocatechuate catabolism bifunctional isomerase/decarboxylase (hpcE)
  6. 2-oxo-hept-4-ene-1,7-dioate hydratase (hpcG)
  7. 4-hydroxy-2-oxo-heptane-1,7-dioate aldolase (hpaI), 4-hydroxy-2-oxo-heptane-1,7-dioate aldolase (hpaI), 4-hydroxy-2-oxo-heptane-1,7-dioate aldolase (hpaI), 4-hydroxy-2-oxo-heptane-1,7-dioate aldolase (hpcH), 4-hydroxy-2-oxo-heptane-1,7-dioate aldolase (hpcH), 4-hydroxy-2-oxo-heptane-1,7-dioate aldolase (hpcH), 4-hydroxy-2-oxo-heptane-1,7-dioate aldolase (hpcH), 4-hydroxy-2-oxo-heptane-1,7-dioate aldolase (hpcH), 4-hydroxy-2-oxo-heptane-1,7-dioate aldolase (hpcH), 4-hydroxy-2-oxo-heptane-1,7-dioate aldolase (hpcH), 4-hydroxy-2-oxo-heptane-1,7-dioate aldolase (hpcH), 4-hydroxy-2-oxo-heptane-1,7-dioate aldolase (hpcH), 4-hydroxy-2-oxo-heptane-1,7-dioate aldolase (hpcH), 4-hydroxy-2-oxo-heptane-1,7-dioate aldolase (hpaI), 4-hydroxy-2-oxo-heptane-1,7-dioate aldolase (hpcH), 4-hydroxy-2-oxo-heptane-1,7-dioate aldolase (hpcH), 4-hydroxy-2-oxo-heptane-1,7-dioate aldolase (hpcH), 4-hydroxy-2-oxo-heptane-1,7-dioate aldolase (hpaI), 4-hydroxy-2-oxo-heptane-1,7-dioate aldolase (hpaI), 4-hydroxy-2-oxo-heptane-1,7-dioate aldolase (yfaU), 4-hydroxy-2-oxo-heptane-1,7-dioate aldolase (hpaI), 4-hydroxy-2-oxo-heptane-1,7-dioate aldolase (hpcH), 4-hydroxy-2-oxo-heptane-1,7-dioate aldolase (hpaI), 4-hydroxy-2-oxo-heptane-1,7-dioate aldolase (hpaI), 4-hydroxy-2-oxo-heptane-1,7-dioate aldolase (hpaI), 4-hydroxy-2-oxo-heptane-1,7-dioate aldolase (hpaI)
This subpathway is part of the pathway 4-hydroxyphenylacetate degradation, which is itself part of Aromatic compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate and succinate semialdehyde from 4-hydroxyphenylacetate, the pathway 4-hydroxyphenylacetate degradation and in Aromatic compound metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
Biological processAromatic hydrocarbons catabolism
LigandFAD, Flavoprotein

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-2841
BRENDAi1.14.14.9 2026
UniPathwayiUPA00208; UER00416

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxyphenylacetate 3-monooxygenase oxygenase component (EC:1.14.14.9)
Alternative name(s):
4-HPA 3-hydroxylase
4-HPA 3-monooxygenase large component
Gene namesi
Name:hpaB
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000840321 – 5204-hydroxyphenylacetate 3-monooxygenase oxygenase componentAdd BLAST520

Proteomic databases

PRIDEiQ57160

Expressioni

Inductioni

By 4-hydroxyphenylacetic acid.

Interactioni

Subunit structurei

4-HPA 3-monooxygenase consists of a reductase component HpaC and an oxygenase component HpaB.

Structurei

3D structure databases

SMRiQ57160
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105EFA Bacteria
COG2368 LUCA

Family and domain databases

InterProiView protein in InterPro
IPR036250 AcylCo_DH-like_C
IPR009100 AcylCoA_DH/oxidase_NM_dom
IPR024677 HpaB/PvcC
IPR004925 HpaB/PvcC/4-BUDH
IPR024719 HpaB/PvcC/4-BUDH_C
IPR024674 HpaB/PvcC/4-BUDH_N
IPR012688 HpaB_gammaproteobact
PANTHERiPTHR36117 PTHR36117, 1 hit
PfamiView protein in Pfam
PF03241 HpaB, 1 hit
PF11794 HpaB_N, 1 hit
PIRSFiPIRSF500125 4_HPA_large, 1 hit
PIRSF000331 HpaA_HpaB, 1 hit
SUPFAMiSSF47203 SSF47203, 1 hit
SSF56645 SSF56645, 1 hit
TIGRFAMsiTIGR02310 HpaB-2, 1 hit

Sequencei

Sequence statusi: Complete.

Q57160-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKPEDFRAST QRPFTGEEYL KSLQDGREIY IYGERVKDVT THPAFRNAAA
60 70 80 90 100
SVAQLYDALH KPEMQDSLCW NTDTGSGGYT HKFFRVAKSA DDLRHERDAI
110 120 130 140 150
AEWSRLSYGW MGRTPDYKAA FGCALGGTPG FYGQFEQNAR NWYTRIQETG
160 170 180 190 200
LYFNHAIVNP PIDRHLPTDK VKDVYIKLEK ETDAGIIVSG AKVVATNSAL
210 220 230 240 250
THYNMIGFGS AQVMGENPDF ALMFVAPMDA DGVKLISRAS YEMVAGATGS
260 270 280 290 300
PYDYPLSSRF DENDAILVMD NVLIPWENVL LYRDFDRCRR WTMEGGFARM
310 320 330 340 350
YPLQACVRLA VKLDFITALL KKSLECTGTL EFRGVQADLG EVVAWRNTFW
360 370 380 390 400
ALSDSMCSEA TPWVNGAYLP DHAALQTYRV LAPMAYAKIK NIIERNVTSG
410 420 430 440 450
LIYLPSSARD LNNPQIDQYL AKYVRGSNGM DHVQRIKILK LMWDAIGSEF
460 470 480 490 500
GGRHELYEIN YSGSQDEIRL QCLRQAQSSG NMDKMMAMVD RCLSEYDQNG
510 520
WTVPHLHNND DINMLDKLLK
Length:520
Mass (Da):58,848
Last modified:November 1, 1996 - v1
Checksum:i1E93BBCC29BBB55C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z37980 Genomic DNA Translation: CAA86048.1
Z29081 Genomic DNA Translation: CAA82321.1
PIRiB55349

Similar proteinsi

Entry informationi

Entry nameiHPAB_ECOLX
AccessioniPrimary (citable) accession number: Q57160
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 2, 2002
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 83 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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