Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Pyrophosphate-energized membrane proton pump 2

Gene

AVPL1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Caution

It is uncertain whether Met-1, Met-2 or Met-3 is the initiator.Curated

Catalytic activityi

Diphosphate + H2O = 2 phosphate.

Enzyme regulationi

Activated by Mg+ but not by K+. Inhibited by Ca2+.1 Publication

Kineticsi

  1. KM=90 µM for PPi (at pH 8 and 37 degrees Celsius)1 Publication
  1. Vmax=0.5 µmol/min/mg enzyme (at pH 8 and 37 degrees Celsius)1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei273SubstrateBy similarity1
Metal bindingi276Magnesium 1By similarity1
Metal bindingi276Magnesium 2By similarity1
Metal bindingi280Magnesium 1By similarity1
Metal bindingi306Magnesium 3By similarity1
Sitei310Important for proton transportBy similarity1
Sitei317Important for proton transportBy similarity1
Metal bindingi541Magnesium 3By similarity1
Metal bindingi568Magnesium 4By similarity1
Metal bindingi743Magnesium 4By similarity1
Metal bindingi773Magnesium 2By similarity1
Binding sitei776SubstrateBy similarity1
Sitei777Important for proton transportBy similarity1
Sitei788Important for proton transportBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processHydrogen ion transport, Ion transport, Transport
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT1G78920-MONOMER
BRENDAi3.6.1.1 399

Protein family/group databases

TCDBi3.A.10.2.3 the h(+), na(+)-translocating pyrophosphatase (m(+)-ppase) family

Names & Taxonomyi

Protein namesi
Recommended name:
Pyrophosphate-energized membrane proton pump 2 (EC:3.6.1.1)
Alternative name(s):
AVP1-like protein 1
Pyrophosphate-energized inorganic pyrophosphatase 2
Short name:
H(+)-PPase 2
Vacuolar proton pyrophosphatase 2
Gene namesi
Name:AVPL1
Synonyms:AVP2
Ordered Locus Names:At1g78920
ORF Names:F9K20.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G78920
TAIRilocus:2037543 AT1G78920

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei45 – 65HelicalSequence analysisAdd BLAST21
Transmembranei66 – 86HelicalSequence analysisAdd BLAST21
Transmembranei118 – 138HelicalSequence analysisAdd BLAST21
Transmembranei160 – 180HelicalSequence analysisAdd BLAST21
Transmembranei206 – 226HelicalSequence analysisAdd BLAST21
Transmembranei246 – 266HelicalSequence analysisAdd BLAST21
Transmembranei348 – 368HelicalSequence analysisAdd BLAST21
Transmembranei386 – 406HelicalSequence analysisAdd BLAST21
Transmembranei421 – 441HelicalSequence analysisAdd BLAST21
Transmembranei468 – 488HelicalSequence analysisAdd BLAST21
Transmembranei511 – 531HelicalSequence analysisAdd BLAST21
Transmembranei577 – 597HelicalSequence analysisAdd BLAST21
Transmembranei615 – 635HelicalSequence analysisAdd BLAST21
Transmembranei686 – 706HelicalSequence analysisAdd BLAST21
Transmembranei716 – 736HelicalSequence analysisAdd BLAST21
Transmembranei782 – 802HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002170401 – 802Pyrophosphate-energized membrane proton pump 2Add BLAST802

Proteomic databases

PaxDbiQ56ZN6
PRIDEiQ56ZN6

PTM databases

SwissPalmiQ56ZN6

Expressioni

Tissue specificityi

Ubiquitous. Mostly expressed in cotyledons, roots and flowers. Especially high levels in trichomes, sepals and stamen filaments.2 Publications

Gene expression databases

ExpressionAtlasiQ56ZN6 baseline and differential
GenevisibleiQ56ZN6 AT

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi29450, 29 interactors
STRINGi3702.AT1G78920.1

Structurei

3D structure databases

ProteinModelPortaliQ56ZN6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IFIU Eukaryota
COG3808 LUCA
HOGENOMiHOG000007098
InParanoidiQ56ZN6
OMAiSIIAMFD
OrthoDBiEOG093603VQ
PhylomeDBiQ56ZN6

Family and domain databases

HAMAPiMF_01129 PPase_energized_pump, 1 hit
InterProiView protein in InterPro
IPR004131 PPase-energised_H-pump
PANTHERiPTHR31998 PTHR31998, 1 hit
PfamiView protein in Pfam
PF03030 H_PPase, 1 hit
PIRSFiPIRSF001265 H+-PPase, 1 hit
TIGRFAMsiTIGR01104 V_PPase, 1 hit

Sequencei

Sequence statusi: Complete.

Q56ZN6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMMDEDVEQA SLMSFNDRPR AFPNMRSKTY SPLIFRIIRK LNVRVLSIIL
60 70 80 90 100
LFCFGAIFYM GASTSPIIVF VFTVCIISFL LSIYLTKWVL AKDEGPPEMV
110 120 130 140 150
EISDAIRDGA EGFFRTQYST ISKMAILLAF VILCIYLFRS LTPQQEAAGL
160 170 180 190 200
GRAMSAYITV AAFLLGALCS GIAGYVGMWV SVRANVRVSS AARRSAREAL
210 220 230 240 250
QIAVRAGGFS ALVVVGMAVI GIAILYSTFY VWLGVGSPGS MNVTDLPLLL
260 270 280 290 300
VGYGFGASFV ALFAQLGGGI YTKGADVGAD LVGKVEQGIP EDDPRNPAVI
310 320 330 340 350
ADLVGDNVGD CAARGADLFE SIAAEIISAM ILGGTMAKKC KIEDPSGFIL
360 370 380 390 400
FPLVVHSFDL IISSIGILSI KGTRDASVKS PVEDPMAVLQ KGYSLTIILA
410 420 430 440 450
VITFGASTRW LLYTEQAPSA WFNFALCGLV GIITAYIFVW ISKYYTDYKH
460 470 480 490 500
EPVRTLALAS STGHGTNIIA GVSLGLESTA LPVLTISVAI ISAYWLGNTS
510 520 530 540 550
GLVDENGIPT GGLFGTAVAT MGMLSTAAYV LTMDMFGPIA DNAGGIVEMS
560 570 580 590 600
QQPESVREIT DLLDAVGNTT KATTKGFAIG SAALASFLLF SAYMDEVSAF
610 620 630 640 650
ANVSFKEVDI AIPEVFVGGL LGAMLIFLFS AWACAAVGRT AQEVVNEVRR
660 670 680 690 700
QFIERPGIME YKEKPDYSRC VAIVASAALR EMIKPGALAI ASPIVVGLVF
710 720 730 740 750
RILGYYTGQP LLGAKVVASM LMFATVCGIL MALFLNTAGG AWDNAKKYIE
760 770 780 790 800
TGALGGKGSE AHKAAVTGDT VGDPFKDTAG PSIHVLIKML ATITLVMAPV

FL
Length:802
Mass (Da):85,133
Last modified:December 6, 2005 - v2
Checksum:iE9C4891A33DD65BD
GO

Sequence cautioni

The sequence AAC83018 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAF31163 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAF31164 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAD94402 differs from that shown. Reason: Frameshift at position 301.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF182813 mRNA Translation: AAF31163.1 Different initiation.
AF184917 Genomic DNA Translation: AAF31164.1 Different initiation.
AB034696 mRNA Translation: BAA92151.1
AC005679 Genomic DNA Translation: AAC83018.1 Sequence problems.
CP002684 Genomic DNA Translation: AEE36177.1
CP002684 Genomic DNA Translation: AEE36178.1
CP002684 Genomic DNA Translation: ANM60408.1
AY054485 mRNA Translation: AAK96676.1
BT010353 mRNA Translation: AAQ56796.1
AK220927 mRNA Translation: BAD94402.1 Sequence problems.
PIRiH96818
RefSeqiNP_001117619.1, NM_001124147.1
NP_001322698.1, NM_001334854.1
NP_565195.1, NM_106541.4
UniGeneiAt.474
At.70012

Genome annotation databases

EnsemblPlantsiAT1G78920.1; AT1G78920.1; AT1G78920
AT1G78920.2; AT1G78920.2; AT1G78920
AT1G78920.3; AT1G78920.3; AT1G78920
GeneIDi844231
GrameneiAT1G78920.1; AT1G78920.1; AT1G78920
AT1G78920.2; AT1G78920.2; AT1G78920
AT1G78920.3; AT1G78920.3; AT1G78920
KEGGiath:AT1G78920

Similar proteinsi

Entry informationi

Entry nameiAVP2_ARATH
AccessioniPrimary (citable) accession number: Q56ZN6
Secondary accession number(s): Q9LDJ4, Q9MB70, Q9ZVB1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: May 23, 2018
This is version 102 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health