Q56YU0 (AL2C4_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aldehyde dehydrogenase family 2 member C4
EC=1.2.1.3

Alternative name(s):
ALDH1a
Protein REDUCED EPIDERMAL FLUORESCENCE 1

Gene names

Name:	ALDH2C4
Synonyms:	REF1
Ordered Locus Names:	At3g24503
ORF Names:	MOB24.3

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	501 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in ferulic acid and sinapic acid biosynthesis by oxidation of conyferylaldehyde and sinapaldehyde, respectively. Can oxidize L-lactaldehyde. Possesses activity on acetaldehyde and glycolaldehyde in vitro. Ref.1 Ref.6
Catalytic activity	An aldehyde + NAD+ + H2O = a carboxylate + NADH.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm › cytosol Potential.
Post-translational modification	Ubiquitinated.
Sequence similarities	Belongs to the aldehyde dehydrogenase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	phenylpropanoid biosynthetic process Inferred from direct assay Ref.6. Source: TAIR
Cellular_component	cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	aldehyde dehydrogenase (NAD) activity Inferred from sequence or structural similarity Ref.1. Source: TAIR coniferyl-aldehyde dehydrogenase activity Inferred from direct assay Ref.6. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 501	501	Aldehyde dehydrogenase family 2 member C4		PRO_0000256058
Regions
	Nucleotide binding	245 – 250	6	NAD By similarity
Sites
	Active site	268	1	Proton acceptor By similarity
	Active site	302	1	Nucleophile By similarity
	Site	169	1	Transition state stabilizer By similarity
Experimental info
	Mutagenesis	152	1	G → E in ref1-7; reduced activity on sinapaldehyde. Ref.6
	Mutagenesis	416	1	G → R in ref1-6; reduced activity on sinapaldehyde. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Q56YU0 [UniParc]. Last modified October 31, 2006. Version 2. Checksum: 3E93A166B1D3ECF6	FASTA	501	54,360
	10 20 30 40 50 60 MENGKCNGAT TVKLPEIKFT KLFINGQFID AASGKTFETI DPRNGEVIAT IAEGDKEDVD 70 80 90 100 110 120 LAVNAARYAF DHGPWPRMTG FERAKLINKF ADLIEENIEE LAKLDAVDGG KLFQLGKYAD 130 140 150 160 170 180 IPATAGHFRY NAGAADKIHG ETLKMTRQSL FGYTLKEPIG VVGNIIPWNF PSIMFATKVA 190 200 210 220 230 240 PAMAAGCTMV VKPAEQTSLS ALFYAHLSKE AGIPDGVLNI VTGFGSTAGA AIASHMDVDK 250 260 270 280 290 300 VSFTGSTDVG RKIMQAAAAS NLKKVSLELG GKSPLLIFND ADIDKAADLA LLGCFYNKGE 310 320 330 340 350 360 ICVASSRVFV QEGIYDKVVE KLVEKAKDWT VGDPFDSTAR QGPQVDKRQF EKILSYIEHG 370 380 390 400 410 420 KNEGATLLTG GKAIGDKGYF IQPTIFADVT EDMKIYQDEI FGPVMSLMKF KTVEEGIKCA 430 440 450 460 470 480 NNTKYGLAAG ILSQDIDLIN TVSRSIKAGI IWVNCYFGFD LDCPYGGYKM SGNCRESGMD 490 500 ALDNYLQTKS VVMPLHNSPW M

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the aldehyde dehydrogenase gene families of Zea mays and Arabidopsis." Skibbe D.S., Liu F., Wen T.-J., Yandeau M.D., Cui X., Cao J., Simmons C.R., Schnable P.S. Plant Mol. Biol. 48:751-764(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones." Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S. DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 322-501. Strain: cv. Columbia.
[6]	"The Arabidopsis thaliana REDUCED EPIDERMAL FLUORESCENCE1 gene encodes an aldehyde dehydrogenase involved in ferulic acid and sinapic acid biosynthesis." Nair R.B., Bastress K.L., Ruegger M.O., Denault J.W., Chapple C. Plant Cell 16:544-554(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF GLY-152 AND GLY-416.
[7]	"The ALDH gene superfamily of Arabidopsis." Kirch H.-H., Bartels D., Wei Y., Schnable P.S., Wood A.J. Trends Plant Sci. 9:371-377(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE.
[8]	"Tandem affinity purification and mass spectrometric analysis of ubiquitylated proteins in Arabidopsis." Saracco S.A., Hansson M., Scalf M., Walker J.M., Smith L.M., Vierstra R.D. Plant J. 59:344-358(2009) [PubMed] [Europe PMC] [Abstract] Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF349448 mRNA. Translation: AAM27004.1. AB020746 Genomic DNA. Translation: BAB01998.1. CP002686 Genomic DNA. Translation: AEE76907.1. AY056398 mRNA. Translation: AAL08254.1. AK221230 mRNA. Translation: BAD93825.1.
IPI	IPI00543086.
RefSeq	NP_566749.1. NM_113359.3.
UniGene	At.22894.
3D structure databases
HSSP	HSSP built from PDB template 1ZUM based on UniProtKB P05091.
ProteinModelPortal	Q56YU0.
SMR	Q56YU0. Positions 15-485.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q56YU0. 1 interaction.
STRING	3702.AT3G24503.1-P.
Proteomic databases
PaxDb	Q56YU0.
PRIDE	Q56YU0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G24503.1; AT3G24503.1; AT3G24503.
GeneID	822042.
KEGG	ath:AT3G24503.
Organism-specific databases
TAIR	At3g24503.
Phylogenomic databases
eggNOG	COG1012.
HOGENOM	HOG000271505.
InParanoid	Q56YU0.
KO	K12355.
OMA	EPFMAEV.
PhylomeDB	Q56YU0.
ProtClustDB	PLN02766.
Gene expression databases
Genevestigator	Q56YU0.
Family and domain databases
Gene3D	3.40.309.10. 1 hit. 3.40.605.10. 1 hit.
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH_dom. [Graphical view]
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
SUPFAM	SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AL2C4_ARATH

Accession

Primary (citable) accession number: Q56YU0
Secondary accession number(s): Q9LV57

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	October 31, 2006
Last modified:	May 1, 2013
This is version 58 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program