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Q56YU0 (AL2C4_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Aldehyde dehydrogenase family 2 member C4

EC=1.2.1.3
Alternative name(s):
ALDH1a
Protein REDUCED EPIDERMAL FLUORESCENCE 1
Gene names
Name:ALDH2C4
Synonyms:REF1
Ordered Locus Names:At3g24503
ORF Names:MOB24.3
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length501 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in ferulic acid and sinapic acid biosynthesis by oxidation of conyferylaldehyde and sinapaldehyde, respectively. Can oxidize L-lactaldehyde. Possesses activity on acetaldehyde and glycolaldehyde in vitro. Ref.1 Ref.6

Catalytic activity

An aldehyde + NAD+ + H2O = a carboxylate + NADH.

Subunit structure

Homotetramer By similarity.

Subcellular location

Cytoplasmcytosol Potential.

Post-translational modification

Ubiquitinated.

Sequence similarities

Belongs to the aldehyde dehydrogenase family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandNAD
   Molecular functionOxidoreductase
   PTMUbl conjugation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processphenylpropanoid biosynthetic process

Inferred from direct assay Ref.6. Source: TAIR

   Cellular_componentcytosol

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionaldehyde dehydrogenase (NAD) activity

Inferred from sequence or structural similarity Ref.1. Source: TAIR

coniferyl-aldehyde dehydrogenase activity

Inferred from direct assay Ref.6. Source: TAIR

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 501501Aldehyde dehydrogenase family 2 member C4
PRO_0000256058

Regions

Nucleotide binding245 – 2506NAD By similarity

Sites

Active site2681Proton acceptor By similarity
Active site3021Nucleophile By similarity
Site1691Transition state stabilizer By similarity

Experimental info

Mutagenesis1521G → E in ref1-7; reduced activity on sinapaldehyde. Ref.6
Mutagenesis4161G → R in ref1-6; reduced activity on sinapaldehyde. Ref.6

Sequences

Sequence LengthMass (Da)Tools
Q56YU0 [UniParc].

Last modified October 31, 2006. Version 2.
Checksum: 3E93A166B1D3ECF6

FASTA50154,360
        10         20         30         40         50         60 
MENGKCNGAT TVKLPEIKFT KLFINGQFID AASGKTFETI DPRNGEVIAT IAEGDKEDVD 

        70         80         90        100        110        120 
LAVNAARYAF DHGPWPRMTG FERAKLINKF ADLIEENIEE LAKLDAVDGG KLFQLGKYAD 

       130        140        150        160        170        180 
IPATAGHFRY NAGAADKIHG ETLKMTRQSL FGYTLKEPIG VVGNIIPWNF PSIMFATKVA 

       190        200        210        220        230        240 
PAMAAGCTMV VKPAEQTSLS ALFYAHLSKE AGIPDGVLNI VTGFGSTAGA AIASHMDVDK 

       250        260        270        280        290        300 
VSFTGSTDVG RKIMQAAAAS NLKKVSLELG GKSPLLIFND ADIDKAADLA LLGCFYNKGE 

       310        320        330        340        350        360 
ICVASSRVFV QEGIYDKVVE KLVEKAKDWT VGDPFDSTAR QGPQVDKRQF EKILSYIEHG 

       370        380        390        400        410        420 
KNEGATLLTG GKAIGDKGYF IQPTIFADVT EDMKIYQDEI FGPVMSLMKF KTVEEGIKCA 

       430        440        450        460        470        480 
NNTKYGLAAG ILSQDIDLIN TVSRSIKAGI IWVNCYFGFD LDCPYGGYKM SGNCRESGMD 

       490        500 
ALDNYLQTKS VVMPLHNSPW M 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of the aldehyde dehydrogenase gene families of Zea mays and Arabidopsis."
Skibbe D.S., Liu F., Wen T.-J., Yandeau M.D., Cui X., Cao J., Simmons C.R., Schnable P.S.
Plant Mol. Biol. 48:751-764(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
[2]"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 322-501.
Strain: cv. Columbia.
[6]"The Arabidopsis thaliana REDUCED EPIDERMAL FLUORESCENCE1 gene encodes an aldehyde dehydrogenase involved in ferulic acid and sinapic acid biosynthesis."
Nair R.B., Bastress K.L., Ruegger M.O., Denault J.W., Chapple C.
Plant Cell 16:544-554(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF GLY-152 AND GLY-416.
[7]"The ALDH gene superfamily of Arabidopsis."
Kirch H.-H., Bartels D., Wei Y., Schnable P.S., Wood A.J.
Trends Plant Sci. 9:371-377(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NOMENCLATURE.
[8]"Tandem affinity purification and mass spectrometric analysis of ubiquitylated proteins in Arabidopsis."
Saracco S.A., Hansson M., Scalf M., Walker J.M., Smith L.M., Vierstra R.D.
Plant J. 59:344-358(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF349448 mRNA. Translation: AAM27004.1.
AB020746 Genomic DNA. Translation: BAB01998.1.
CP002686 Genomic DNA. Translation: AEE76907.1.
AY056398 mRNA. Translation: AAL08254.1.
AK221230 mRNA. Translation: BAD93825.1.
RefSeqNP_566749.1. NM_113359.3.
UniGeneAt.22894.

3D structure databases

ProteinModelPortalQ56YU0.
SMRQ56YU0. Positions 8-498.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid7373. 3 interactions.
IntActQ56YU0. 1 interaction.
STRING3702.AT3G24503.1-P.

Proteomic databases

PaxDbQ56YU0.
PRIDEQ56YU0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G24503.1; AT3G24503.1; AT3G24503.
GeneID822042.
KEGGath:AT3G24503.

Organism-specific databases

TAIRAT3G24503.

Phylogenomic databases

eggNOGCOG1012.
HOGENOMHOG000271505.
InParanoidQ56YU0.
KOK12355.
OMAIGREMGH.
PhylomeDBQ56YU0.
ProtClustDBPLN02766.

Gene expression databases

GenevestigatorQ56YU0.

Family and domain databases

Gene3D3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
[Graphical view]
PfamPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMSSF53720. SSF53720. 1 hit.
PROSITEPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAL2C4_ARATH
AccessionPrimary (citable) accession number: Q56YU0
Secondary accession number(s): Q9LV57
Entry history
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: October 31, 2006
Last modified: March 19, 2014
This is version 65 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names