Reviewed, UniProtKB/Swiss-Prot Q56YU0 (AL2C4_ARATH)

Names and origin

Protein names

Recommended name:
    Aldehyde dehydrogenase family 2 member C4
    EC=1.2.1.3
Alternative name(s):
    ALDH1a
    Protein REDUCED EPIDERMAL FLUORESCENCE 1

Gene names

Name:	ALDH2C4
Synonyms:	REF1
Ordered Locus Names:	At3g24503
ORF Names:	MOB24.3

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	501 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in ferulic acid and sinapic acid biosynthesis by oxidation of conyferylaldehyde and sinapaldehyde, respectively. Can oxidize L-lactaldehyde. Possesses activity on acetaldehyde and glycolaldehyde in vitro. Ref.1 Ref.5
Catalytic activity	An aldehyde + NAD+ + H2O = an acid + NADH.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm › cytosol Potential.
Sequence similarities	Belongs to the aldehyde dehydrogenase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW phenylpropanoid biosynthetic process Ref.5 Inferred from direct assay. Source: TAIR
Cellular component	cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aldehyde dehydrogenase (NAD) activity Inferred from electronic annotation. Source: EC coniferyl-aldehyde dehydrogenase activity Ref.5 Inferred from direct assay. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 501	501	Aldehyde dehydrogenase family 2 member C4		PRO_0000256058
Regions
	Nucleotide binding	245 – 250	6	NAD By similarity
Sites
	Active site	268	1	Proton acceptor By similarity
	Active site	302	1	Nucleophile By similarity
	Site	169	1	Transition state stabilizer By similarity
Experimental info
	Mutagenesis	152	1	G → E in ref1-7; reduced activity on sinapaldehyde. Ref.5
	Mutagenesis	416	1	G → R in ref1-6; reduced activity on sinapaldehyde. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q56YU0-1 [UniParc]. Last modified October 31, 2006. Version 2. Checksum: 3E93A166B1D3ECF6	FASTA	501	54,360
	10 20 30 40 50 60 MENGKCNGAT TVKLPEIKFT KLFINGQFID AASGKTFETI DPRNGEVIAT IAEGDKEDVD 70 80 90 100 110 120 LAVNAARYAF DHGPWPRMTG FERAKLINKF ADLIEENIEE LAKLDAVDGG KLFQLGKYAD 130 140 150 160 170 180 IPATAGHFRY NAGAADKIHG ETLKMTRQSL FGYTLKEPIG VVGNIIPWNF PSIMFATKVA 190 200 210 220 230 240 PAMAAGCTMV VKPAEQTSLS ALFYAHLSKE AGIPDGVLNI VTGFGSTAGA AIASHMDVDK 250 260 270 280 290 300 VSFTGSTDVG RKIMQAAAAS NLKKVSLELG GKSPLLIFND ADIDKAADLA LLGCFYNKGE 310 320 330 340 350 360 ICVASSRVFV QEGIYDKVVE KLVEKAKDWT VGDPFDSTAR QGPQVDKRQF EKILSYIEHG 370 380 390 400 410 420 KNEGATLLTG GKAIGDKGYF IQPTIFADVT EDMKIYQDEI FGPVMSLMKF KTVEEGIKCA 430 440 450 460 470 480 NNTKYGLAAG ILSQDIDLIN TVSRSIKAGI IWVNCYFGFD LDCPYGGYKM SGNCRESGMD 490 500 ALDNYLQTKS VVMPLHNSPW M

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the aldehyde dehydrogenase gene families of Zea mays and Arabidopsis." Skibbe D.S., Liu F., Wen T.-J., Yandeau M.D., Cui X., Cao J., Simmons C.R., Schnable P.S. Plant Mol. Biol. 48:751-764(2002) [PubMed: 11999848] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones." Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S. DNA Res. 7:217-221(2000) [PubMed: 10907853] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 322-501. Strain: cv. Columbia.
[5]	"The Arabidopsis thaliana REDUCED EPIDERMAL FLUORESCENCE1 gene encodes an aldehyde dehydrogenase involved in ferulic acid and sinapic acid biosynthesis." Nair R.B., Bastress K.L., Ruegger M.O., Denault J.W., Chapple C. Plant Cell 16:544-554(2004) [PubMed: 14729911] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF GLY-152 AND GLY-416.
[6]	"The ALDH gene superfamily of Arabidopsis." Kirch H.-H., Bartels D., Wei Y., Schnable P.S., Wood A.J. Trends Plant Sci. 9:371-377(2004) [PubMed: 15358267] [Abstract] Cited for: NOMENCLATURE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF349448 mRNA. Translation: AAM27004.1. AB020746 Genomic DNA. Translation: BAB01998.1. AY056398 mRNA. Translation: AAL08254.1. AK221230 mRNA. Translation: BAD93825.1.
IPI	IPI00543086.
RefSeq	NP_566749.1.
UniGene	At.22894
3D structure databases
HSSP	HSSP built from PDB template 1O04 based on UniProtKB P05091.
ModBase	Search...
Proteomic databases
PRIDE	Q56YU0.
ProMEX	Q56YU0.
Genome annotation databases
GeneID	822042.
GenomeReviews	Gene locus AT3G24503 in contig BA000014_GR.
KEGG	ath:AT3G24503.
NMPDR	fig|3702.1.peg.14712.
Organism-specific databases
TAIR	At3g24503.
Phylogenomic databases
OMA	Q56YU0. SALFYAH.
Enzyme and pathway databases
BRENDA	1.2.1.3. 302.
Family and domain databases
InterPro	IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AL2C4_ARATH

Accession

Primary (citable) accession number: Q56YU0
Secondary accession number(s): Q9LV57

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	October 31, 2006
Last modified:	June 16, 2009
This is version 27 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)