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Q56YP2

- PI5K1_ARATH

UniProt

Q56YP2 - PI5K1_ARATH

Protein

Phosphatidylinositol 4-phosphate 5-kinase 1

Gene

PIP5K1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 1 (10 May 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the synthesis of phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 3,4-bisphosphate.1 Publication

    Catalytic activityi

    ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate.

    GO - Molecular functioni

    1. 1-phosphatidylinositol-4-phosphate 5-kinase activity Source: TAIR
    2. actin filament binding Source: TAIR
    3. actin monomer binding Source: TAIR
    4. ATP binding Source: UniProtKB-KW
    5. phosphatidylinositol phosphate kinase activity Source: TAIR

    GO - Biological processi

    1. phosphatidylinositol phosphorylation Source: GOC
    2. response to stress Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Stress response

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT1G21980-MONOMER.
    ReactomeiREACT_187642. Synthesis of PIPs at the plasma membrane.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphatidylinositol 4-phosphate 5-kinase 1 (EC:2.7.1.68)
    Short name:
    AtPIP5K1
    Alternative name(s):
    1-phosphatidylinositol 4-phosphate kinase 1
    Diphosphoinositide kinase 1
    PtdIns(4)P-5-kinase 1
    Gene namesi
    Name:PIP5K1
    Synonyms:P5K1
    Ordered Locus Names:At1g21980
    ORF Names:F2E2.1
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G21980.

    Subcellular locationi

    GO - Cellular componenti

    1. plasma membrane Source: TAIR

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 752752Phosphatidylinositol 4-phosphate 5-kinase 1PRO_0000185473Add
    BLAST

    Post-translational modificationi

    Phosphorylation inactivates the enzyme.1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiQ56YP2.

    Expressioni

    Tissue specificityi

    Expressed in the whole plant, preferentially in roots. Strongly expressed in meristematic tissues, namely procambial cell layers.2 Publications

    Inductioni

    By abscisic acid (ABA), drought and salt treatment.1 Publication

    Gene expression databases

    ArrayExpressiQ56YP2.
    GenevestigatoriQ56YP2.

    Interactioni

    Protein-protein interaction databases

    BioGridi24040. 6 interactions.
    MINTiMINT-8067725.
    STRINGi3702.AT1G21980.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ56YP2.
    SMRiQ56YP2. Positions 410-602.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati81 – 10323MORN 1Add
    BLAST
    Repeati104 – 12623MORN 2Add
    BLAST
    Repeati127 – 14923MORN 3Add
    BLAST
    Repeati150 – 17223MORN 4Add
    BLAST
    Repeati173 – 19523MORN 5Add
    BLAST
    Repeati196 – 21823MORN 6Add
    BLAST
    Repeati219 – 24123MORN 7Add
    BLAST
    Domaini349 – 748400PIPKPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni708 – 72922Activation loopBy similarityAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi5 – 128Poly-Glu

    Sequence similaritiesi

    Contains 7 MORN repeats.Curated
    Contains 1 PIPK domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG4642.
    HOGENOMiHOG000193875.
    InParanoidiQ56YP2.
    KOiK00889.
    OMAiDNTGEKM.
    PhylomeDBiQ56YP2.

    Family and domain databases

    Gene3Di3.30.800.10. 1 hit.
    3.30.810.10. 2 hits.
    InterProiIPR003409. MORN.
    IPR017163. PIno-4-P-5_kinase_pln.
    IPR023610. PInositol-4-P-5-kinase.
    IPR027483. PInositol-4-P-5-kinase_C.
    IPR002498. PInositol-4-P-5-kinase_core.
    IPR027484. PInositol-4-P-5-kinase_N.
    IPR016034. PInositol-4P-5-kinase_core_sub.
    [Graphical view]
    PANTHERiPTHR23086. PTHR23086. 1 hit.
    PfamiPF02493. MORN. 7 hits.
    PF01504. PIP5K. 1 hit.
    [Graphical view]
    PIRSFiPIRSF037274. PIP5K_plant_prd. 1 hit.
    SMARTiSM00698. MORN. 7 hits.
    SM00330. PIPKc. 1 hit.
    [Graphical view]
    PROSITEiPS51455. PIPK. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q56YP2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDSEEDEEE EEASEVILSS VVQKKKKKNL RFGEEVERRD GLVLLAQSTP    50
    MVRSRSQGTT RRVTPTPLVD VEKPLPNGDL YIGSFSGGFP HGSGKYLWKD 100
    GCMYEGDWKR GKASGKGKFS WPSGATYEGE FKSGRMEGFG TFTGADGDTY 150
    RGTWVADRKH GHGQKRYANG DFYEGTWRRN LQDGRGRYVW RNGNQYTGEW 200
    RSGVISGKGL LVWPNGNRYE GLWENGIPKG NGVFTWSDGS SCVGAWNESN 250
    IMRSFFNGVE KNDLIVGNRK RSSVDSGAGS LGGEKVFPRI CIWESDGEAG 300
    DITCDIIDNV EASMIYRDRI SVDRDGFRQF KKNPCWFNGE AKKPGQTISK 350
    GHKKYDLMLN LQLGIRYSVG KHASIVRDLK QTDFDPKEKF WTRFPPEGTK 400
    TTPPHQSVDF RWKDYCPLVF RRLRELFQVD PAKYMLAICG NDALRELSSP 450
    GKSGSFFYLT QDDRFMIKTV KKSEVKVLLR MLPSYYKHVC QYENSLVTRF 500
    YGVHCVKPVG GQKTRFIVMG NLFCSEYRIQ RRFDLKGSSH GRSTAKPEGE 550
    IDETTTLKDL DLNFSFRLQR NWYQELMKQI KRDCEFLEAE RIMDYSLLVG 600
    VHFRDDNTGE KMGLSPFVLR SGRIDSYQNE KFMRGCRFLE AELQDMDRIL 650
    AGRKPSIRLG ANMPAKAERM ARRSDFDQYS SGGASYPSHG EMYEVVLYFG 700
    VIDILQDYDI TKKIEHAYKS LQADPASISA VDPKLYSKRF RDFISRIFIE 750
    EG 752
    Length:752
    Mass (Da):85,945
    Last modified:May 10, 2005 - v1
    Checksum:i8A9E7D10A32CBF53
    GO

    Sequence cautioni

    The sequence BAA33501.1 differs from that shown. Reason: Frameshift at position 91.
    The sequence AAF86542.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti182 – 1821Q → P in AAB82658. 1 PublicationCurated
    Sequence conflicti186 – 1861G → S in AAB82658. 1 PublicationCurated
    Sequence conflicti202 – 2021S → I in BAA33501. (PubMed:9753781)Curated
    Sequence conflicti416 – 4161C → Y in BAA33501. (PubMed:9753781)Curated
    Sequence conflicti419 – 4191V → M in BAA33501. (PubMed:9753781)Curated
    Sequence conflicti456 – 4561F → L in BAA33501. (PubMed:9753781)Curated
    Sequence conflicti588 – 5881E → D in BAA33501. (PubMed:9753781)Curated
    Sequence conflicti602 – 6021H → P in BAA33501. (PubMed:9753781)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF019380 mRNA. Translation: AAB82658.1.
    AB005902 mRNA. Translation: BAA33501.1. Frameshift.
    AC069252 Genomic DNA. Translation: AAF86542.1. Sequence problems.
    CP002684 Genomic DNA. Translation: AEE30181.1.
    AK221279 mRNA. Translation: BAD93975.1.
    PIRiT51821.
    RefSeqiNP_173617.1. NM_102047.3.
    UniGeneiAt.22532.

    Genome annotation databases

    EnsemblPlantsiAT1G21980.1; AT1G21980.1; AT1G21980.
    GeneIDi838801.
    KEGGiath:AT1G21980.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF019380 mRNA. Translation: AAB82658.1 .
    AB005902 mRNA. Translation: BAA33501.1 . Frameshift.
    AC069252 Genomic DNA. Translation: AAF86542.1 . Sequence problems.
    CP002684 Genomic DNA. Translation: AEE30181.1 .
    AK221279 mRNA. Translation: BAD93975.1 .
    PIRi T51821.
    RefSeqi NP_173617.1. NM_102047.3.
    UniGenei At.22532.

    3D structure databases

    ProteinModelPortali Q56YP2.
    SMRi Q56YP2. Positions 410-602.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 24040. 6 interactions.
    MINTi MINT-8067725.
    STRINGi 3702.AT1G21980.1-P.

    Proteomic databases

    PRIDEi Q56YP2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G21980.1 ; AT1G21980.1 ; AT1G21980 .
    GeneIDi 838801.
    KEGGi ath:AT1G21980.

    Organism-specific databases

    GeneFarmi 5099.
    TAIRi AT1G21980.

    Phylogenomic databases

    eggNOGi COG4642.
    HOGENOMi HOG000193875.
    InParanoidi Q56YP2.
    KOi K00889.
    OMAi DNTGEKM.
    PhylomeDBi Q56YP2.

    Enzyme and pathway databases

    BioCyci ARA:AT1G21980-MONOMER.
    Reactomei REACT_187642. Synthesis of PIPs at the plasma membrane.

    Gene expression databases

    ArrayExpressi Q56YP2.
    Genevestigatori Q56YP2.

    Family and domain databases

    Gene3Di 3.30.800.10. 1 hit.
    3.30.810.10. 2 hits.
    InterProi IPR003409. MORN.
    IPR017163. PIno-4-P-5_kinase_pln.
    IPR023610. PInositol-4-P-5-kinase.
    IPR027483. PInositol-4-P-5-kinase_C.
    IPR002498. PInositol-4-P-5-kinase_core.
    IPR027484. PInositol-4-P-5-kinase_N.
    IPR016034. PInositol-4P-5-kinase_core_sub.
    [Graphical view ]
    PANTHERi PTHR23086. PTHR23086. 1 hit.
    Pfami PF02493. MORN. 7 hits.
    PF01504. PIP5K. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF037274. PIP5K_plant_prd. 1 hit.
    SMARTi SM00698. MORN. 7 hits.
    SM00330. PIPKc. 1 hit.
    [Graphical view ]
    PROSITEi PS51455. PIPK. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "An Arabidopsis phosphatidylinositol-4-phosphate 5-kinase homolog with seven novel repeats rich in aromatic and glycine residues."
      Satterlee J.S., Sussman M.R.
      Plant Gene Register PGR97-150
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Columbia.
    2. "A gene encoding phosphatidylinositol-4-phosphate 5-kinase is induced by water stress and abscisic acid in Arabidopsis thaliana."
      Mikami K., Katagiri T., Iuchi S., Yamaguchi-Shinozaki K., Shinozaki K.
      Plant J. 15:563-568(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION.
      Strain: cv. Columbia.
    3. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    6. "AtPIP5K1, an Arabidopsis thaliana phosphatidylinositol phosphate kinase, synthesizes PtdIns(3,4)P(2) and PtdIns(4,5)P(2) in vitro and is inhibited by phosphorylation."
      Westergren T., Dove S.K., Sommarin M., Pical C.
      Biochem. J. 359:583-589(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, PHOSPHORYLATION.
    7. "An Arabidopsis inositol phospholipid kinase strongly expressed in procambial cells: synthesis of PtdIns(4,5)P2 and PtdIns(3,4,5)P3 in insect cells by 5-phosphorylation of precursors."
      Elge S., Brearley C., Xia H.J., Kehr J., Xue H.W., Mueller-Roeber B.
      Plant J. 26:561-571(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION, TISSUE SPECIFICITY.
    8. "Inositol phospholipid metabolism in Arabidopsis. Characterized and putative isoforms of inositol phospholipid kinase and phosphoinositide-specific phospholipase C."
      Mueller-Roeber B., Pical C.
      Plant Physiol. 130:22-46(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.

    Entry informationi

    Entry nameiPI5K1_ARATH
    AccessioniPrimary (citable) accession number: Q56YP2
    Secondary accession number(s): O22503, O82120, Q9LM65
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 25, 2005
    Last sequence update: May 10, 2005
    Last modified: October 1, 2014
    This is version 79 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3