Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Phosphatidylinositol 4-phosphate 5-kinase 1

Gene

PIP5K1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the synthesis of phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 3,4-bisphosphate.1 Publication

Catalytic activityi

ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate.

GO - Molecular functioni

  • 1-phosphatidylinositol-4-phosphate 5-kinase activity Source: TAIR
  • actin filament binding Source: TAIR
  • actin monomer binding Source: TAIR
  • ATP binding Source: UniProtKB-KW
  • phosphatidylinositol phosphate kinase activity Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Stress response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G21980-MONOMER.
BRENDAi2.7.1.68. 399.
ReactomeiR-ATH-1660499. Synthesis of PIPs at the plasma membrane.
R-ATH-6811558. PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling.
R-ATH-8856828. Clathrin-mediated endocytosis.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol 4-phosphate 5-kinase 1 (EC:2.7.1.68)
Short name:
AtPIP5K1
Alternative name(s):
1-phosphatidylinositol 4-phosphate kinase 1
Diphosphoinositide kinase 1
PtdIns(4)P-5-kinase 1
Gene namesi
Name:PIP5K1
Synonyms:P5K1
Ordered Locus Names:At1g21980
ORF Names:F2E2.1
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G21980.

Subcellular locationi

GO - Cellular componenti

  • plasma membrane Source: TAIR
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001854731 – 752Phosphatidylinositol 4-phosphate 5-kinase 1Add BLAST752

Post-translational modificationi

Phosphorylation inactivates the enzyme.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ56YP2.

PTM databases

iPTMnetiQ56YP2.

Expressioni

Tissue specificityi

Expressed in the whole plant, preferentially in roots. Strongly expressed in meristematic tissues, namely procambial cell layers.2 Publications

Inductioni

By abscisic acid (ABA), drought and salt treatment.1 Publication

Gene expression databases

GenevisibleiQ56YP2. AT.

Interactioni

GO - Molecular functioni

  • actin filament binding Source: TAIR
  • actin monomer binding Source: TAIR

Protein-protein interaction databases

BioGridi24040. 7 interactors.
MINTiMINT-8067725.
STRINGi3702.AT1G21980.1.

Structurei

3D structure databases

ProteinModelPortaliQ56YP2.
SMRiQ56YP2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati81 – 103MORN 1Add BLAST23
Repeati104 – 126MORN 2Add BLAST23
Repeati127 – 149MORN 3Add BLAST23
Repeati150 – 172MORN 4Add BLAST23
Repeati173 – 195MORN 5Add BLAST23
Repeati196 – 218MORN 6Add BLAST23
Repeati219 – 241MORN 7Add BLAST23
Domaini349 – 748PIPKPROSITE-ProRule annotationAdd BLAST400

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni708 – 729Activation loopBy similarityAdd BLAST22

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi5 – 12Poly-Glu8

Sequence similaritiesi

Contains 7 MORN repeats.Curated
Contains 1 PIPK domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0229. Eukaryota.
COG4642. LUCA.
COG5253. LUCA.
HOGENOMiHOG000193875.
InParanoidiQ56YP2.
KOiK00889.
OMAiKQIKRDC.
OrthoDBiEOG0936025Q.
PhylomeDBiQ56YP2.

Family and domain databases

Gene3Di3.30.800.10. 1 hit.
3.30.810.10. 2 hits.
InterProiIPR003409. MORN.
IPR023610. PInositol-4-P-5-kinase.
IPR027483. PInositol-4-P-5-kinase_C.
IPR002498. PInositol-4-P-5-kinase_core.
IPR027484. PInositol-4-P-5-kinase_N.
[Graphical view]
PANTHERiPTHR23086. PTHR23086. 1 hit.
PfamiPF02493. MORN. 7 hits.
PF01504. PIP5K. 1 hit.
[Graphical view]
SMARTiSM00698. MORN. 7 hits.
SM00330. PIPKc. 1 hit.
[Graphical view]
PROSITEiPS51455. PIPK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q56YP2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDSEEDEEE EEASEVILSS VVQKKKKKNL RFGEEVERRD GLVLLAQSTP
60 70 80 90 100
MVRSRSQGTT RRVTPTPLVD VEKPLPNGDL YIGSFSGGFP HGSGKYLWKD
110 120 130 140 150
GCMYEGDWKR GKASGKGKFS WPSGATYEGE FKSGRMEGFG TFTGADGDTY
160 170 180 190 200
RGTWVADRKH GHGQKRYANG DFYEGTWRRN LQDGRGRYVW RNGNQYTGEW
210 220 230 240 250
RSGVISGKGL LVWPNGNRYE GLWENGIPKG NGVFTWSDGS SCVGAWNESN
260 270 280 290 300
IMRSFFNGVE KNDLIVGNRK RSSVDSGAGS LGGEKVFPRI CIWESDGEAG
310 320 330 340 350
DITCDIIDNV EASMIYRDRI SVDRDGFRQF KKNPCWFNGE AKKPGQTISK
360 370 380 390 400
GHKKYDLMLN LQLGIRYSVG KHASIVRDLK QTDFDPKEKF WTRFPPEGTK
410 420 430 440 450
TTPPHQSVDF RWKDYCPLVF RRLRELFQVD PAKYMLAICG NDALRELSSP
460 470 480 490 500
GKSGSFFYLT QDDRFMIKTV KKSEVKVLLR MLPSYYKHVC QYENSLVTRF
510 520 530 540 550
YGVHCVKPVG GQKTRFIVMG NLFCSEYRIQ RRFDLKGSSH GRSTAKPEGE
560 570 580 590 600
IDETTTLKDL DLNFSFRLQR NWYQELMKQI KRDCEFLEAE RIMDYSLLVG
610 620 630 640 650
VHFRDDNTGE KMGLSPFVLR SGRIDSYQNE KFMRGCRFLE AELQDMDRIL
660 670 680 690 700
AGRKPSIRLG ANMPAKAERM ARRSDFDQYS SGGASYPSHG EMYEVVLYFG
710 720 730 740 750
VIDILQDYDI TKKIEHAYKS LQADPASISA VDPKLYSKRF RDFISRIFIE

EG
Length:752
Mass (Da):85,945
Last modified:May 10, 2005 - v1
Checksum:i8A9E7D10A32CBF53
GO

Sequence cautioni

The sequence AAF86542 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence BAA33501 differs from that shown. Reason: Frameshift at position 91.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti182Q → P in AAB82658 (Ref. 1) Curated1
Sequence conflicti186G → S in AAB82658 (Ref. 1) Curated1
Sequence conflicti202S → I in BAA33501 (PubMed:9753781).Curated1
Sequence conflicti416C → Y in BAA33501 (PubMed:9753781).Curated1
Sequence conflicti419V → M in BAA33501 (PubMed:9753781).Curated1
Sequence conflicti456F → L in BAA33501 (PubMed:9753781).Curated1
Sequence conflicti588E → D in BAA33501 (PubMed:9753781).Curated1
Sequence conflicti602H → P in BAA33501 (PubMed:9753781).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF019380 mRNA. Translation: AAB82658.1.
AB005902 mRNA. Translation: BAA33501.1. Frameshift.
AC069252 Genomic DNA. Translation: AAF86542.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE30181.1.
AK221279 mRNA. Translation: BAD93975.1.
PIRiT51821.
RefSeqiNP_173617.1. NM_102047.4.
UniGeneiAt.22532.

Genome annotation databases

EnsemblPlantsiAT1G21980.1; AT1G21980.1; AT1G21980.
GeneIDi838801.
GrameneiAT1G21980.1; AT1G21980.1; AT1G21980.
KEGGiath:AT1G21980.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF019380 mRNA. Translation: AAB82658.1.
AB005902 mRNA. Translation: BAA33501.1. Frameshift.
AC069252 Genomic DNA. Translation: AAF86542.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE30181.1.
AK221279 mRNA. Translation: BAD93975.1.
PIRiT51821.
RefSeqiNP_173617.1. NM_102047.4.
UniGeneiAt.22532.

3D structure databases

ProteinModelPortaliQ56YP2.
SMRiQ56YP2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi24040. 7 interactors.
MINTiMINT-8067725.
STRINGi3702.AT1G21980.1.

PTM databases

iPTMnetiQ56YP2.

Proteomic databases

PaxDbiQ56YP2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G21980.1; AT1G21980.1; AT1G21980.
GeneIDi838801.
GrameneiAT1G21980.1; AT1G21980.1; AT1G21980.
KEGGiath:AT1G21980.

Organism-specific databases

TAIRiAT1G21980.

Phylogenomic databases

eggNOGiKOG0229. Eukaryota.
COG4642. LUCA.
COG5253. LUCA.
HOGENOMiHOG000193875.
InParanoidiQ56YP2.
KOiK00889.
OMAiKQIKRDC.
OrthoDBiEOG0936025Q.
PhylomeDBiQ56YP2.

Enzyme and pathway databases

BioCyciARA:AT1G21980-MONOMER.
BRENDAi2.7.1.68. 399.
ReactomeiR-ATH-1660499. Synthesis of PIPs at the plasma membrane.
R-ATH-6811558. PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling.
R-ATH-8856828. Clathrin-mediated endocytosis.

Miscellaneous databases

PROiQ56YP2.

Gene expression databases

GenevisibleiQ56YP2. AT.

Family and domain databases

Gene3Di3.30.800.10. 1 hit.
3.30.810.10. 2 hits.
InterProiIPR003409. MORN.
IPR023610. PInositol-4-P-5-kinase.
IPR027483. PInositol-4-P-5-kinase_C.
IPR002498. PInositol-4-P-5-kinase_core.
IPR027484. PInositol-4-P-5-kinase_N.
[Graphical view]
PANTHERiPTHR23086. PTHR23086. 1 hit.
PfamiPF02493. MORN. 7 hits.
PF01504. PIP5K. 1 hit.
[Graphical view]
SMARTiSM00698. MORN. 7 hits.
SM00330. PIPKc. 1 hit.
[Graphical view]
PROSITEiPS51455. PIPK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPI5K1_ARATH
AccessioniPrimary (citable) accession number: Q56YP2
Secondary accession number(s): O22503, O82120, Q9LM65
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: May 10, 2005
Last modified: November 30, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.